FastQCFastQC Report
Sat 14 Jan 2017
SRR2934578.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934578.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240054
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28781.198896914860823No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15770.6569355228406941No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.2736884201054763No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.1495496846542861No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG253.881557E-545.0000042
CGCATGG253.881557E-545.0000042
TCGATGG253.881557E-545.0000042
ACAACGG207.0221274E-445.02
TAGGTAA207.0221274E-445.06
TGGCGAA207.0221274E-445.01
ACGGGCC207.0221274E-445.05
AGTGCGG207.0221274E-445.02
CGAGGAT207.0221274E-445.05
CGTTTAG207.0221274E-445.02
GTAACGG207.0221274E-445.02
AAAGCCG207.0221274E-445.045
CGCTTAG207.0221274E-445.02
TCACGAC302.1583037E-644.99999625
CGTTGTT302.1583037E-644.9999961
CACGACG302.1583037E-644.99999626
GACGGTC302.1583037E-644.99999629
ACGGTCT302.1583037E-644.99999630
CGTTTTA7750.044.4193531
CGTTATT2450.044.0816351
Produced by FastQC (version 0.11.5)