##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934578.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240054
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.651424262874187	31.0	31.0	34.0	30.0	34.0
2	31.836640922459114	31.0	31.0	34.0	30.0	34.0
3	31.217071992135104	31.0	31.0	34.0	28.0	34.0
4	35.292425870845726	37.0	35.0	37.0	33.0	37.0
5	31.820532046956103	37.0	35.0	37.0	0.0	37.0
6	33.790263857298775	37.0	35.0	37.0	19.0	37.0
7	21.08690128054521	32.0	0.0	37.0	0.0	37.0
8	28.311129995750957	35.0	17.0	37.0	17.0	37.0
9	34.93057395419364	37.0	32.0	39.0	32.0	39.0
10	36.102751880826816	37.0	35.0	39.0	32.0	39.0
11	36.56554358602648	38.0	35.0	39.0	32.0	39.0
12	36.996225849183936	39.0	37.0	39.0	34.0	39.0
13	36.836024394511234	39.0	37.0	39.0	33.0	39.0
14	38.25415531505411	40.0	38.0	41.0	34.0	41.0
15	38.41346530364002	40.0	38.0	41.0	34.0	41.0
16	38.470489973089386	40.0	38.0	41.0	34.0	41.0
17	38.45398951902489	40.0	38.0	41.0	34.0	41.0
18	38.27954543561032	40.0	37.0	41.0	34.0	41.0
19	38.118794104659784	40.0	37.0	41.0	34.0	41.0
20	37.97236038557991	40.0	36.0	41.0	34.0	41.0
21	37.88145583910287	39.0	36.0	41.0	33.0	41.0
22	37.80521049430545	39.0	36.0	41.0	33.0	41.0
23	37.68747031917818	39.0	35.0	41.0	33.0	41.0
24	37.52458613478634	39.0	35.0	41.0	33.0	41.0
25	37.38409691152824	39.0	35.0	41.0	33.0	41.0
26	37.245536420971945	39.0	35.0	41.0	33.0	41.0
27	37.19752222416623	39.0	35.0	41.0	33.0	41.0
28	37.13064560473893	39.0	35.0	41.0	33.0	41.0
29	37.11133744907396	39.0	35.0	41.0	33.0	41.0
30	36.94343772651153	39.0	35.0	40.0	32.0	41.0
31	36.72331225474268	39.0	35.0	40.0	31.0	41.0
32	36.46254592716639	39.0	35.0	40.0	30.0	41.0
33	36.11521157739509	39.0	35.0	40.0	30.0	41.0
34	35.72287068742866	39.0	35.0	40.0	26.0	41.0
35	35.35482016546277	39.0	35.0	40.0	24.0	41.0
36	35.0701425512593	39.0	35.0	40.0	22.0	41.0
37	34.892132603497544	39.0	34.0	40.0	21.0	41.0
38	34.790222200005	39.0	34.0	40.0	21.0	41.0
39	34.708961317037	39.0	34.0	40.0	20.0	41.0
40	34.635094603714165	38.0	34.0	40.0	19.0	41.0
41	34.57411249135611	38.0	34.0	40.0	18.0	41.0
42	34.42979912852942	38.0	34.0	40.0	18.0	41.0
43	34.32312729635832	38.0	34.0	40.0	18.0	41.0
44	34.17294858656802	38.0	33.0	40.0	17.0	41.0
45	34.11692785789864	38.0	33.0	40.0	18.0	41.0
46	33.99866696659918	38.0	33.0	40.0	17.0	41.0
47	33.981254217800995	38.0	33.0	40.0	17.0	41.0
48	33.864409674489906	38.0	33.0	40.0	15.0	41.0
49	33.739912686312245	38.0	33.0	40.0	15.0	41.0
50	33.533471635548665	38.0	33.0	40.0	14.0	41.0
51	31.860872970248362	36.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	14.0
10	11.0
11	7.0
12	4.0
13	4.0
14	4.0
15	8.0
16	22.0
17	20.0
18	88.0
19	169.0
20	351.0
21	670.0
22	1263.0
23	2110.0
24	3393.0
25	4818.0
26	5504.0
27	5299.0
28	4852.0
29	4770.0
30	5248.0
31	6278.0
32	7977.0
33	11678.0
34	15844.0
35	20826.0
36	25757.0
37	42357.0
38	43034.0
39	27670.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.452439867696434	21.339365309472036	22.456613928532747	20.751580894298783
2	38.12892099277663	23.452223249768803	23.152290734584717	15.266565022869855
3	27.260116473793396	22.948169995084438	34.63845634732185	15.153257183800312
4	23.877127646279586	24.048755696634924	35.45993818057604	16.61417847650945
5	19.983420397077325	35.08793854716023	30.728502753547122	14.200138302215334
6	22.811117498562826	31.59372474526565	33.31125496763228	12.283902788539246
7	45.461021270214204	41.46067134894649	10.94753680421905	2.1307705766202605
8	79.2250910211869	3.4971298124588635	15.099102701892075	2.178676464462163
9	74.14415089938096	6.390228865172003	16.355486682163182	3.1101335532838443
10	34.70594116323827	26.957684520982777	26.017895973405984	12.318478342372966
11	25.681721612637155	24.58321877577545	33.30292350887717	16.432136102710224
12	23.01357194631208	22.624076249510527	36.994176310330175	17.368175493847218
13	19.586009814458414	26.571521407683274	37.60653852883101	16.2359302490273
14	17.644779924516985	28.52524848575737	37.22495771784682	16.605013871878825
15	16.91577728344456	26.744399176851875	40.97703016821215	15.362793371491415
16	19.752222416622928	24.272871937147475	39.47236871703866	16.50253692919093
17	20.248777358427684	24.497821323535536	35.925666724986876	19.327734593049897
18	21.087755255067613	24.648204154065333	37.23037316603764	17.033667424829414
19	20.2608579736226	27.494230464812087	35.22207503311755	17.022836528447765
20	20.228781857415417	26.537778999725063	35.951077674189975	17.28236146866955
21	19.042382130687262	26.52694810334341	37.960625525923334	16.47004424004599
22	19.56226515700634	24.0874969798462	36.17810992526682	20.172127937880642
23	17.20154631874495	27.01100585701551	36.65550251193481	19.131945312304733
24	17.803494213801898	26.040390912044792	37.91521907570797	18.24089579844535
25	17.319436460129804	28.00078315712298	36.435135427862065	18.24464495488515
26	17.331100502386963	29.400468227982035	35.57157972789456	17.696851541736443
27	17.43149458038608	28.846842793704752	36.687578628142006	17.03408399776717
28	16.454631041349028	27.914135986069798	38.18640805818691	17.44482491439426
29	17.146975263898955	27.50214535062944	37.626534029843285	17.72434535562832
30	17.805993651428427	27.964541311538238	36.510118556658085	17.719346480375247
31	19.062377631699533	27.987036250177045	35.169170270022576	17.781415848100842
32	18.540411740691678	28.002449448874007	35.37579044714939	18.081348363284928
33	19.452706474376598	28.028276971014854	34.50307014255126	18.01594641205729
34	17.289443208611395	28.175743790980363	35.15417364426337	19.38063935614487
35	18.453347996700742	28.421938397193962	34.5280645188166	18.596649087288693
36	20.588284302698558	29.002224499487617	32.486857123813806	17.922634074000015
37	18.284635956909696	30.2202837694852	33.71074841493997	17.784331858665134
38	19.390220533713247	29.83537037499896	31.95822606580186	18.816183025485934
39	19.636831712864605	28.939321985886508	32.47061077924134	18.953235522007546
40	20.037574878985563	28.254059503278427	31.916568772026295	19.791796845709715
41	19.200679847034415	28.60648020861973	31.52332391878494	20.669516025560917
42	20.00216617927633	29.05179667908054	31.155073441808927	19.790963699834204
43	19.487698601148075	29.499196014230133	31.33586609679489	19.677239287826907
44	18.661634465578576	29.923267264865405	31.573312671315623	19.841785598240396
45	18.60373082723054	30.523132295233573	30.45189832287735	20.421238554658537
46	19.61225390953702	30.357752838944567	30.563956443133627	19.46603680838478
47	18.462096028393614	29.64916227182217	31.692035958575985	20.19670574120823
48	19.3831387937714	28.533163371574727	32.251076841044096	19.83262099360977
49	19.973839219508942	27.11931482083198	32.49143942612912	20.415406533529957
50	18.322544094245462	28.653136377648362	32.19400634857157	20.830313179534603
51	18.911161655294226	28.471094003849135	30.671432261074592	21.94631207978205
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	284.0
1	425.0
2	566.0
3	4490.5
4	8415.0
5	6016.5
6	3618.0
7	3198.0
8	2778.0
9	2679.5
10	2581.0
11	2499.0
12	2417.0
13	2325.5
14	2234.0
15	2196.0
16	2158.0
17	2036.5
18	1915.0
19	1890.5
20	1866.0
21	1910.5
22	1955.0
23	2088.0
24	2221.0
25	2464.5
26	3102.5
27	3497.0
28	4058.5
29	4620.0
30	5461.5
31	6303.0
32	7142.5
33	7982.0
34	8671.0
35	9360.0
36	10164.5
37	10969.0
38	12072.5
39	13176.0
40	14329.0
41	15482.0
42	16309.5
43	17137.0
44	17842.0
45	18547.0
46	19188.0
47	19829.0
48	19710.5
49	19592.0
50	18310.5
51	17029.0
52	14629.0
53	12229.0
54	10613.0
55	8997.0
56	7869.0
57	6741.0
58	5709.5
59	4678.0
60	3960.5
61	3243.0
62	2664.5
63	2086.0
64	1678.0
65	1270.0
66	985.5
67	701.0
68	553.0
69	405.0
70	329.0
71	253.0
72	188.0
73	123.0
74	81.5
75	31.5
76	23.0
77	18.5
78	14.0
79	9.0
80	4.0
81	3.5
82	3.0
83	3.0
84	3.0
85	1.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	240054.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.25600315117718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.34230824067306	44.072251392577975
2	8.917153694660959	10.032868526927563
3	4.040331775387392	6.8187875126398305
4	2.344422582949789	5.27551376856457
5	1.4652502050346972	4.121456007584746
6	1.0595877283479251	3.576490235093372
7	0.8167238622538464	3.2161934118015787
8	0.6310462970731665	2.8400113981349397
9	0.5065934635363544	2.564903113296022
>10	1.8579579193843885	14.504309896085447
>50	0.013409446102890502	0.4823724891082948
>100	0.0029798769117534447	0.3524546162815603
>500	7.449692279383612E-4	0.2753420724102082
>1k	0.0014899384558767223	1.8670455594938775
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2878	1.198896914860823	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1577	0.6569355228406941	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	657	0.2736884201054763	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	359	0.1495496846542861	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03874128321127746	0.0
2	0.0	0.0	0.0	0.19703899955843268	0.0
3	0.0	0.0	0.0	0.30159880693510627	0.0
4	0.0	0.0	0.0	0.4011597390587118	0.0
5	0.0	0.0	0.0	0.6835961908570571	0.0
6	0.0	0.0	0.0	0.9131278795604323	0.0
7	0.0	0.0	0.0	1.1401601306372733	0.0
8	0.0	0.0	0.0	1.6867038249727144	0.0
9	0.0	0.0	0.0	1.8883251268464596	0.0
10	0.0	0.0	0.0	2.3973772567838902	0.0
11	0.0	0.0	0.0	3.193031567897223	0.0
12	0.0	0.0	0.0	3.8053937863980605	0.0
13	0.0	0.0	0.0	4.086163946445383	0.0
14	0.0	0.0	0.0	4.215301557149641	0.0
15	0.0	0.0	0.0	4.391511909820291	0.0
16	0.0	0.0	0.0	4.80641855582494	0.0
17	0.0	0.0	0.0	5.327551300957285	0.0
18	0.0	0.0	0.0	5.903255100935623	0.0
19	0.0	0.0	0.0	6.283169620168795	0.0
20	0.0	0.0	0.0	6.63975605488765	0.0
21	0.0	0.0	0.0	7.125480100310764	0.0
22	0.0	0.0	0.0	7.6062052704808085	0.0
23	0.0	0.0	0.0	8.167329017637698	0.0
24	0.0	0.0	0.0	8.572654486073967	0.0
25	0.0	0.0	0.0	8.930490639606088	0.0
26	0.0	0.0	0.0	9.282494772009631	0.0
27	0.0	0.0	0.0	9.617419413965191	0.0
28	0.0	0.0	0.0	10.012747131895324	0.0
29	0.0	0.0	0.0	10.402242828696876	0.0
30	0.0	0.0	0.0	10.814233464137235	0.0
31	0.0	0.0	0.0	11.282461446174612	0.0
32	0.0	0.0	0.0	11.7223624684446	0.0
33	0.0	0.0	0.0	12.140601697951295	0.0
34	0.0	0.0	0.0	12.553425479267165	0.0
35	0.0	0.0	0.0	12.99374307447491	0.0
36	0.0	0.0	0.0	13.41239887691936	0.0
37	0.0	0.0	0.0	13.82897181467503	0.0
38	0.0	0.0	0.0	14.253876211185817	0.0
39	0.0	0.0	0.0	14.703358411024187	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	25	3.881557E-5	45.000004	2
CGCATGG	25	3.881557E-5	45.000004	2
TCGATGG	25	3.881557E-5	45.000004	2
ACAACGG	20	7.0221274E-4	45.0	2
TAGGTAA	20	7.0221274E-4	45.0	6
TGGCGAA	20	7.0221274E-4	45.0	1
ACGGGCC	20	7.0221274E-4	45.0	5
AGTGCGG	20	7.0221274E-4	45.0	2
CGAGGAT	20	7.0221274E-4	45.0	5
CGTTTAG	20	7.0221274E-4	45.0	2
GTAACGG	20	7.0221274E-4	45.0	2
AAAGCCG	20	7.0221274E-4	45.0	45
CGCTTAG	20	7.0221274E-4	45.0	2
TCACGAC	30	2.1583037E-6	44.999996	25
CGTTGTT	30	2.1583037E-6	44.999996	1
CACGACG	30	2.1583037E-6	44.999996	26
GACGGTC	30	2.1583037E-6	44.999996	29
ACGGTCT	30	2.1583037E-6	44.999996	30
CGTTTTA	775	0.0	44.419353	1
CGTTATT	245	0.0	44.081635	1
>>END_MODULE