##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934577.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	671618
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77525915029079	31.0	31.0	34.0	30.0	34.0
2	31.958628267854643	33.0	31.0	34.0	30.0	34.0
3	31.170217296141555	31.0	31.0	34.0	27.0	34.0
4	35.31926779806378	37.0	35.0	37.0	33.0	37.0
5	31.793130916681804	37.0	35.0	37.0	0.0	37.0
6	33.7859423064897	37.0	35.0	37.0	17.0	37.0
7	21.18898987221903	32.0	0.0	37.0	0.0	37.0
8	28.318761557909408	35.0	17.0	37.0	17.0	37.0
9	34.950632651298804	37.0	32.0	39.0	32.0	39.0
10	36.383889353769554	37.0	35.0	39.0	32.0	39.0
11	36.832379418062054	39.0	37.0	39.0	33.0	39.0
12	37.15980363837777	39.0	37.0	39.0	34.0	39.0
13	37.00424497258859	39.0	37.0	39.0	33.0	39.0
14	38.43529357462129	40.0	38.0	41.0	34.0	41.0
15	38.59392243805258	40.0	38.0	41.0	34.0	41.0
16	38.635387973520665	40.0	38.0	41.0	35.0	41.0
17	38.60830561420331	40.0	38.0	41.0	34.0	41.0
18	38.39684165701336	40.0	38.0	41.0	34.0	41.0
19	38.22042291898073	40.0	37.0	41.0	34.0	41.0
20	38.03333293628223	40.0	36.0	41.0	34.0	41.0
21	37.91913558004699	40.0	36.0	41.0	33.0	41.0
22	37.809443761185676	39.0	35.0	41.0	33.0	41.0
23	37.66568793570154	39.0	35.0	41.0	33.0	41.0
24	37.44529181767017	39.0	35.0	41.0	33.0	41.0
25	37.26612300444598	39.0	35.0	41.0	33.0	41.0
26	37.14787721591738	39.0	35.0	41.0	33.0	41.0
27	37.09295015916786	39.0	35.0	41.0	33.0	41.0
28	36.985123984169576	39.0	35.0	41.0	33.0	41.0
29	36.93767439228847	39.0	35.0	41.0	33.0	41.0
30	36.73236125297416	39.0	35.0	41.0	32.0	41.0
31	36.518835111625954	39.0	35.0	40.0	31.0	41.0
32	36.23443385972383	39.0	35.0	40.0	30.0	41.0
33	35.80362646623527	38.0	35.0	40.0	27.0	41.0
34	35.30744857940079	38.0	34.0	40.0	24.0	41.0
35	34.83113912968384	38.0	34.0	40.0	21.0	41.0
36	34.493791113400775	38.0	34.0	40.0	19.0	41.0
37	34.311665262098394	38.0	34.0	40.0	18.0	41.0
38	34.11107504563606	38.0	33.0	40.0	16.0	41.0
39	33.970416218743395	38.0	33.0	40.0	15.0	41.0
40	33.819214196165085	38.0	33.0	40.0	15.0	41.0
41	33.656303136604436	38.0	33.0	40.0	15.0	41.0
42	33.51809808551885	38.0	33.0	40.0	13.0	41.0
43	33.399597687971436	38.0	33.0	40.0	12.0	41.0
44	33.236144653657284	38.0	33.0	40.0	12.0	41.0
45	33.13060400406183	37.0	33.0	40.0	11.0	41.0
46	32.99562400054793	37.0	32.0	40.0	10.0	41.0
47	32.94848857535087	37.0	32.0	40.0	10.0	41.0
48	32.82855134913001	37.0	32.0	40.0	10.0	41.0
49	32.71588462489093	37.0	31.0	40.0	10.0	41.0
50	32.564591776873165	36.0	31.0	40.0	10.0	41.0
51	30.976290093475754	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	22.0
9	36.0
10	37.0
11	39.0
12	19.0
13	21.0
14	33.0
15	48.0
16	80.0
17	162.0
18	327.0
19	605.0
20	1143.0
21	1989.0
22	3584.0
23	6191.0
24	10564.0
25	15343.0
26	18690.0
27	18408.0
28	16974.0
29	15907.0
30	16103.0
31	18624.0
32	23191.0
33	32167.0
34	42673.0
35	55463.0
36	68923.0
37	113284.0
38	115642.0
39	75309.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.24756632490493	17.99341292222662	22.37149689257882	24.38752386028963
2	40.699474999181085	21.20416665425882	23.02856683412297	15.067791512437129
3	27.99612279599415	21.304670214318257	36.40685627842017	14.292350711267417
4	23.793287255553007	22.58977573561161	37.13211974664169	16.484817262193687
5	20.283554044114364	33.88533362715115	31.388974089437742	14.442138239296742
6	22.62149019234148	30.742177845144113	34.186546519003365	12.449785443511043
7	43.35068446646754	41.3228948598757	13.111173315783676	2.2152473578730767
8	75.2001882022221	3.899091447817063	18.661352137673497	2.2393682122873417
9	69.92114565124818	6.2021863618902415	19.97936326900113	3.8973047178604503
10	34.49386407154067	27.75759434678642	26.088639673147533	11.65990190852538
11	25.979798039957235	23.424923096164786	35.201855816848266	15.393423047029712
12	23.99369879901968	20.640006670458504	38.5638860185403	16.80240851198151
13	20.867963634089616	21.66365404143427	39.33217989988356	18.13620242459255
14	17.965867502062185	23.051347641069775	39.98642085232974	18.996364004538293
15	17.567426721737654	22.499992555291847	41.15658007974771	18.776000643222783
16	20.80140794320581	22.633699513711665	38.77740024835546	17.787492294727063
17	21.615710120931837	22.11093210723953	37.44479748904884	18.828560282779794
18	21.530542659666654	22.264441989345134	38.167083074009334	18.03793227697888
19	21.477983020109644	23.49847681271199	36.03238745834686	18.991152708831507
20	22.649928977484223	23.386955084586774	37.01613119362494	16.946984744304054
21	21.97186495894988	23.136068419845802	37.48336107727905	17.40870554392527
22	22.70159525206293	20.85396758276282	37.39670467438336	19.047732490790896
23	21.185405989714393	22.613747695862827	37.878675080179505	18.322171234243275
24	19.853547701223018	22.480636314095214	39.10064947633923	18.56516650834254
25	19.764657885881558	24.362062958407904	37.20984249975432	18.663436655956215
26	19.82183324449315	24.340324410602456	36.451077844846324	19.386764500058067
27	19.49635060406362	23.93548118126673	37.20835355812381	19.359814656545833
28	18.420143593530845	23.986998561682384	37.813012754274006	19.77984509051276
29	19.689913016029944	22.973624887957143	37.7175715957583	19.61889050025461
30	20.177839188348138	23.418818435479693	37.564806184467955	18.838536191704215
31	21.24064572420632	23.946201561006404	36.08569156871913	18.72746114606815
32	21.716809257643483	23.613423106587376	35.805174965531	18.864592670238142
33	20.990652424443656	24.08511981513301	35.25113978481815	19.67308797560518
34	20.100265329398557	24.258134832598294	36.601163161201754	19.040436676801395
35	20.61886369930526	25.715659794704727	34.81636882870917	18.849107677280834
36	21.382988544083094	26.4708509896995	33.099916917057016	19.046243549160387
37	20.73261883987624	26.92155362125494	33.268167321304674	19.07766021756415
38	20.63807104633884	27.381636585082592	32.77488095911664	19.205411409461924
39	21.274891381708056	26.317490001756948	32.59754801092288	19.81007060561212
40	21.99539023671194	25.849664541450647	32.684353307981624	19.47059191385579
41	20.02745608366661	26.099806735376358	33.03529685029287	20.83744033066416
42	20.88761766361235	25.976373474207065	32.568662543290976	20.567346318889605
43	20.9014648207761	26.16666021458627	32.496299980048185	20.435574984589454
44	21.256428505489726	25.847728917330983	32.00971385519745	20.88612872198184
45	20.903996021547965	26.297984866397268	31.635542823450237	21.162476288604534
46	21.18064137649676	26.32627475737696	31.439746999038142	21.053336867088138
47	20.234716758633628	26.45804609167711	32.96338096953923	20.343856180150024
48	20.491112507407486	26.08402395409295	32.93375103109208	20.491112507407486
49	20.873770506448604	25.688561057029442	32.813444547346855	20.6242238891751
50	20.309759416811342	26.217433124186666	33.11733753413399	20.355469924868004
51	20.228909886274636	25.48755393691057	32.27504325375436	22.00849292306043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	545.0
1	984.0
2	1423.0
3	18031.0
4	34639.0
5	23567.0
6	12495.0
7	10981.0
8	9467.0
9	9204.0
10	8941.0
11	8572.0
12	8203.0
13	7896.0
14	7589.0
15	7130.0
16	6671.0
17	6261.0
18	5851.0
19	5734.0
20	5617.0
21	5318.0
22	5019.0
23	5222.5
24	5426.0
25	5975.5
26	7304.0
27	8083.0
28	9469.0
29	10855.0
30	12476.5
31	14098.0
32	15889.0
33	17680.0
34	19594.5
35	21509.0
36	23174.5
37	24840.0
38	25922.0
39	27004.0
40	28333.0
41	29662.0
42	31699.0
43	33736.0
44	35014.0
45	36292.0
46	37595.5
47	38899.0
48	38584.0
49	38269.0
50	37362.5
51	36456.0
52	35162.5
53	33869.0
54	33345.0
55	32821.0
56	31749.0
57	30677.0
58	28878.0
59	27079.0
60	25337.5
61	23596.0
62	21610.0
63	19624.0
64	17479.0
65	15334.0
66	13185.5
67	11037.0
68	9481.0
69	7925.0
70	6762.0
71	5599.0
72	4677.5
73	3756.0
74	3011.0
75	1678.0
76	1090.0
77	809.5
78	529.0
79	402.0
80	275.0
81	213.0
82	151.0
83	115.5
84	80.0
85	65.5
86	51.0
87	38.0
88	25.0
89	22.5
90	20.0
91	16.0
92	12.0
93	10.0
94	8.0
95	4.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	671618.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.50940388592445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51582226861068	31.2511378082221
2	7.942075705751579	6.752258077367647
3	4.03753817270084	5.149000226645323
4	2.676232087050485	4.550601227235984
5	2.0229344966420273	4.299686977626261
6	1.7056027721251126	4.350249426553128
7	1.3835509265774706	4.1169747594238695
8	1.2301509548175327	4.183438702319528
9	1.016523725529162	3.8890635847329387
>10	4.432774129014259	26.270357126855764
>50	0.023877451457392115	0.7361363581234668
>100	0.011351575211546257	0.8897199566732024
>500	3.9143362798435365E-4	0.08419642754378912
>1k	3.9143362798435365E-4	0.20017451054383062
>5k	3.9143362798435365E-4	1.2125283942916822
>10k+	3.9143362798435365E-4	2.064476435841485
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12407	1.8473298809740062	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7287	1.0849917661527833	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1203	0.17911967815037716	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4889416305102006E-4	0.0	0.0	0.013549368837642827	0.0
2	1.4889416305102006E-4	0.0	0.0	0.05687757028548967	0.0
3	1.4889416305102006E-4	0.0	0.0	0.10065245422248957	0.0
4	1.4889416305102006E-4	0.0	0.0	0.14472512648559152	0.0
5	1.4889416305102006E-4	0.0	0.0	0.2958527019823769	0.0
6	1.4889416305102006E-4	0.0	0.0	0.4548716681208663	0.0
7	1.4889416305102006E-4	0.0	0.0	0.585749637442713	0.0
8	1.4889416305102006E-4	0.0	0.0	0.8634372515328654	0.0
9	1.4889416305102006E-4	0.0	0.0	1.024242947627967	0.0
10	2.977883261020401E-4	0.0	0.0	1.320095649610344	0.0
11	2.977883261020401E-4	0.0	0.0	1.5306319961644863	0.0
12	2.977883261020401E-4	0.0	0.0	1.7401260835772716	0.0
13	2.977883261020401E-4	0.0	0.0	1.8354183479299244	0.0
14	2.977883261020401E-4	0.0	0.0	1.8924448123784652	0.0
15	2.977883261020401E-4	0.0	0.0	1.9536403133924345	0.0
16	2.977883261020401E-4	0.0	0.0	2.045061329505761	0.0
17	2.977883261020401E-4	0.0	0.0	2.1533073860438523	0.0
18	2.977883261020401E-4	0.0	0.0	2.277485118028403	0.0
19	2.977883261020401E-4	0.0	0.0	2.3544634003257805	0.0
20	4.4668248915306024E-4	0.0	0.0	2.4445443689716475	0.0
21	4.4668248915306024E-4	0.0	0.0	2.538198797530739	0.0
22	4.4668248915306024E-4	0.0	0.0	2.6583563871129123	0.0
23	4.4668248915306024E-4	0.0	0.0	2.7883409914564528	0.0
24	4.4668248915306024E-4	0.0	0.0	2.8965870479945446	0.0
25	4.4668248915306024E-4	0.0	0.0	2.988752534923126	0.0
26	4.4668248915306024E-4	0.0	0.0	3.084491481764932	0.0
27	4.4668248915306024E-4	0.0	0.0	3.1949709507487887	0.0
28	4.4668248915306024E-4	0.0	0.0	3.2983034999061966	0.0
29	4.4668248915306024E-4	0.0	0.0	3.4154832062273495	0.0
30	4.4668248915306024E-4	0.0	0.0	3.560357226875992	0.0
31	4.4668248915306024E-4	0.0	0.0	3.6817059697625734	0.0
32	4.4668248915306024E-4	0.0	0.0	3.80379918346441	0.0
33	4.4668248915306024E-4	0.0	0.0	3.9220211489269197	0.0
34	4.4668248915306024E-4	0.0	0.0	4.057365943140297	0.0
35	4.4668248915306024E-4	0.0	0.0	4.203877799582501	0.0
36	4.4668248915306024E-4	0.0	0.0	4.339222593795878	0.0
37	4.4668248915306024E-4	0.0	0.0	4.487521180194694	0.0
38	4.4668248915306024E-4	0.0	0.0	4.648922452942	0.0
39	4.4668248915306024E-4	0.0	0.0	4.858863222843938	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	30	2.164148E-6	45.000004	2
TTGGCGA	20	7.0311903E-4	45.0	1
GCGCAAA	20	7.0311903E-4	45.0	1
TTACGCG	20	7.0311903E-4	45.0	1
ACCCGTT	20	7.0311903E-4	45.0	43
CGTTTAG	20	7.0311903E-4	45.0	2
CGTTTTA	2820	0.0	44.521275	1
CGGTTTA	230	0.0	44.021736	1
CGTTATT	1195	0.0	43.870293	1
CGTTTTT	5325	0.0	43.394367	1
GTTTTAT	3105	0.0	41.666668	2
CACGACC	50	1.0804797E-9	40.5	27
ACGGGCT	130	0.0	39.807693	5
TAGACGG	40	3.4553523E-7	39.375	2
TACGGGT	40	3.4553523E-7	39.375	4
TTGACGG	80	0.0	39.375	2
AGGCGAT	320	0.0	38.671875	7
ACGCGAG	35	6.2444087E-6	38.57143	2
TTTGACG	35	6.2444087E-6	38.57143	1
TTACACG	245	0.0	38.57143	34
>>END_MODULE