FastQCFastQC Report
Sat 14 Jan 2017
SRR2934576.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934576.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694750
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145542.0948542641237857No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83501.2018711766822598No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31100.44764303706369196No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.2112990284274919No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAA302.1642572E-645.0000041
TCACGAC453.8380676E-1045.025
GCGATCG207.0313574E-445.09
CGTTTTT62550.044.100721
CGTTTTA31250.043.921
CGTTATT14400.043.906251
CGAAGGA1850.041.3513534
GTTTTAT33650.041.1887052
CGGTTTT6350.040.748031
CACGACG501.0804797E-940.50000426
GCGCAAA451.9264917E-840.01
AGGCGAT2850.039.4736867
GGACGTA403.4555887E-739.3758
GTTATTT16350.039.082572
ACAACGA1400.038.5714313
TTGCACG356.2447216E-638.571431
TACGTAG356.2447216E-638.571432
CGGTTTA2100.038.5714261
GGCACCG1200.037.5000048
CGCGAAG301.13945694E-437.5000042
Produced by FastQC (version 0.11.5)