##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934576.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	694750
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.710498740554158	31.0	31.0	34.0	30.0	34.0
2	31.893669665347247	33.0	31.0	34.0	30.0	34.0
3	30.986305865419215	31.0	31.0	34.0	27.0	34.0
4	35.19797768981648	37.0	35.0	37.0	32.0	37.0
5	31.719476070528966	37.0	35.0	37.0	0.0	37.0
6	33.74630154731918	37.0	35.0	37.0	17.0	37.0
7	21.272238934868657	32.0	0.0	37.0	0.0	37.0
8	28.40056135300468	35.0	17.0	37.0	17.0	37.0
9	35.012738395106155	37.0	32.0	39.0	32.0	39.0
10	36.43097373155811	37.0	35.0	39.0	33.0	39.0
11	36.8275480388629	39.0	37.0	39.0	33.0	39.0
12	37.19806549118388	39.0	37.0	39.0	34.0	39.0
13	37.071300467794174	39.0	37.0	39.0	34.0	39.0
14	38.486275638718965	40.0	38.0	41.0	34.0	41.0
15	38.63791723641598	40.0	38.0	41.0	35.0	41.0
16	38.67376322418136	40.0	38.0	41.0	35.0	41.0
17	38.64651169485426	40.0	38.0	41.0	35.0	41.0
18	38.416434688736956	40.0	38.0	41.0	34.0	41.0
19	38.22939474631162	40.0	37.0	41.0	34.0	41.0
20	38.01275854623965	40.0	36.0	41.0	34.0	41.0
21	37.88953580424613	40.0	36.0	41.0	33.0	41.0
22	37.76572436128104	39.0	35.0	41.0	33.0	41.0
23	37.62880892407341	39.0	35.0	41.0	33.0	41.0
24	37.40109535804246	39.0	35.0	41.0	33.0	41.0
25	37.25407412738395	39.0	35.0	41.0	33.0	41.0
26	37.14288449082404	39.0	35.0	41.0	33.0	41.0
27	37.084495142137456	39.0	35.0	41.0	33.0	41.0
28	36.999974091399785	39.0	35.0	41.0	33.0	41.0
29	36.949229219143575	39.0	35.0	41.0	33.0	41.0
30	36.735671824397265	39.0	35.0	41.0	32.0	41.0
31	36.49830586541921	39.0	35.0	40.0	31.0	41.0
32	36.17757898524649	38.0	35.0	40.0	30.0	41.0
33	35.70807340770061	38.0	35.0	40.0	26.0	41.0
34	35.14034256926952	38.0	34.0	40.0	23.0	41.0
35	34.65169197553077	38.0	34.0	40.0	20.0	41.0
36	34.276929830874415	38.0	33.0	40.0	18.0	41.0
37	34.08496581504138	38.0	33.0	40.0	15.0	41.0
38	33.85341345807844	38.0	33.0	40.0	15.0	41.0
39	33.72456135300468	38.0	33.0	40.0	13.0	41.0
40	33.62615473191796	38.0	33.0	40.0	12.0	41.0
41	33.46220942785175	38.0	33.0	40.0	10.0	41.0
42	33.33907160849226	38.0	33.0	40.0	10.0	41.0
43	33.22998920474991	38.0	33.0	40.0	10.0	41.0
44	33.09157682619647	38.0	33.0	40.0	10.0	41.0
45	33.008447643037066	38.0	32.0	40.0	10.0	41.0
46	32.89904569989205	38.0	32.0	40.0	10.0	41.0
47	32.86258078445484	38.0	32.0	40.0	10.0	41.0
48	32.75019503418496	37.0	32.0	40.0	10.0	41.0
49	32.65573515653113	37.0	31.0	40.0	10.0	41.0
50	32.501229219143575	37.0	31.0	40.0	10.0	41.0
51	31.020637639438647	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	17.0
9	31.0
10	48.0
11	26.0
12	22.0
13	23.0
14	22.0
15	53.0
16	68.0
17	142.0
18	317.0
19	625.0
20	1158.0
21	2154.0
22	3960.0
23	6900.0
24	11733.0
25	17453.0
26	20690.0
27	20655.0
28	18514.0
29	16897.0
30	16529.0
31	18505.0
32	22997.0
33	31150.0
34	41582.0
35	54659.0
36	68890.0
37	116241.0
38	121703.0
39	80976.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.883411299028424	18.674199352284994	20.684994602374953	26.757394746311626
2	42.84375674703131	20.1270960777258	22.768621806405182	14.260525368837712
3	27.855631522130263	20.30082763584023	37.76970133141418	14.07383951061533
4	23.43821518531846	22.569845268082044	38.390356243252974	15.601583303346528
5	19.667938107232818	33.680604534005035	32.92263404102195	13.728823317740193
6	22.569845268082044	29.954084202950703	35.93853904282116	11.537531486146095
7	42.48866498740554	41.00626124505218	14.403742353364521	2.101331414177762
8	74.17574667146457	3.3898524649154376	20.4	2.0344008636200073
9	68.69953220582944	6.502338970852825	21.690104354084202	3.1080244692335373
10	32.03540842029507	31.575098956459158	26.982511694854267	9.406980928391508
11	23.10975170924793	22.87974091399784	39.36739834472832	14.643109032025908
12	21.586326016552718	21.81576106513134	40.28326736236056	16.31464555595538
13	20.339402662828356	21.73429291111911	41.654839870457	16.27146455559554
14	17.1273119827276	24.276502338970854	41.659157970492984	16.937027707808564
15	17.025692695214108	23.961856783015474	42.783015473191796	16.229435048578626
16	20.72169845268082	23.672112270600937	40.49715725080964	15.1090320259086
17	20.831234256926955	22.866210867218424	39.55797049298309	16.744584382871537
18	20.590716084922633	22.91284634760705	40.62482907520691	15.871608492263404
19	19.957682619647354	24.08420295070169	39.09982007916516	16.858294350485785
20	20.35235696293631	24.634184958618206	39.57207628643397	15.441381792011516
21	20.277941705649514	23.730694494422455	40.58740554156171	15.403958258366318
22	20.463907880532567	21.35098956459158	40.854839870457	17.330262684418855
23	18.286721842389348	23.64692335372436	40.74933429291112	17.31702051097517
24	17.61237855343649	23.86541921554516	41.71529327096078	16.806908960057573
25	18.788197193234975	24.958618207988483	39.5651673263764	16.688017272400142
26	18.448218783735157	25.42626844188557	38.64800287873336	17.477509895645916
27	17.911910759265922	24.56250449802087	40.16538323137819	17.360201511335013
28	17.00122346167686	24.89960417416337	40.40834832673624	17.690824037423532
29	18.397984886649873	24.03771140698093	40.18985246491544	17.374451241453762
30	18.623533645196115	23.474919035624325	40.105649514213745	17.795897804965815
31	19.539114789492622	23.66275638718964	39.108888089240736	17.689240734077003
32	19.420510975170924	24.791651673263765	38.66038143216984	17.127455919395466
33	19.412018711766823	24.941489744512417	37.99712126664267	17.649370277078084
34	18.20985966174883	25.776034544800286	38.618495861820804	17.395609931630084
35	18.470672903922274	26.378409499820076	37.579704929830875	17.57121266642677
36	19.454767902123066	27.925584742713205	35.039654551997124	17.579992803166608
37	19.419215545160128	27.73342929111191	34.78762144656351	18.05973371716445
38	19.00798848506657	28.771932349766104	34.693342929111196	17.526736236056134
39	19.15163727959698	27.813889888449083	34.24267722202231	18.79179560993163
40	20.704857862540482	26.99877653832314	34.09211946743433	18.20424613170205
41	18.90550557754588	27.558834112990283	33.939690536164086	19.59596977329975
42	19.56977329974811	27.637423533645194	33.92256207268802	18.870241093918676
43	19.69355883411299	27.79186757826556	33.55883411299028	18.955739474631162
44	19.91694854264124	27.514789492623247	33.4809643756747	19.087297589060814
45	19.511910759265923	27.515509175962578	33.12400143936668	19.848578625404823
46	19.22302986685858	27.958402302986684	32.904066210867214	19.914501619287513
47	18.855127743792732	28.168261964735514	33.64980208708168	19.32680820439007
48	19.11536523929471	27.641885570349046	33.857790572148254	19.38495861820799
49	19.61568909679741	26.98452680820439	33.88614609571788	19.513637999280316
50	18.65721482547679	27.769125584742717	33.949478229578986	19.62418136020151
51	19.101979129183157	27.394746311622885	33.34652752788773	20.156747031306224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	608.0
1	959.0
2	1310.0
3	21309.0
4	41308.0
5	28320.5
6	15333.0
7	13332.0
8	11331.0
9	10782.5
10	10234.0
11	9816.0
12	9398.0
13	9027.5
14	8657.0
15	8283.5
16	7910.0
17	7396.5
18	6883.0
19	6481.5
20	6080.0
21	6448.0
22	6816.0
23	7051.5
24	7287.0
25	7968.5
26	9581.5
27	10513.0
28	11991.5
29	13470.0
30	14853.5
31	16237.0
32	18737.0
33	21237.0
34	23444.0
35	25651.0
36	27318.0
37	28985.0
38	30316.0
39	31647.0
40	33471.5
41	35296.0
42	37249.5
43	39203.0
44	40387.0
45	41571.0
46	42523.5
47	43476.0
48	42406.5
49	41337.0
50	40086.0
51	38835.0
52	36509.5
53	34184.0
54	32037.5
55	29891.0
56	27764.0
57	25637.0
58	23105.0
59	20573.0
60	18523.0
61	16473.0
62	14515.5
63	12558.0
64	10762.0
65	8966.0
66	7521.5
67	6077.0
68	5151.5
69	4226.0
70	3427.0
71	2628.0
72	2208.0
73	1788.0
74	1473.0
75	921.0
76	684.0
77	477.0
78	270.0
79	213.0
80	156.0
81	121.0
82	86.0
83	72.5
84	59.0
85	46.5
86	34.0
87	26.5
88	19.0
89	14.0
90	9.0
91	7.5
92	6.0
93	5.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	694750.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.80977271805709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5423936393112	32.65685323198168
2	8.059923659923639	7.062068473322909
3	3.885194421368307	5.106284536968266
4	2.549609856462088	4.467913133252889
5	1.9423759027692982	4.254752341667696
6	1.6036936492673242	4.215447257027581
7	1.332474800374806	4.086279270987111
8	1.1428472775290326	4.005430358399778
9	0.926392537360791	3.6526541858532413
>10	3.9774368767128614	24.38264879815461
>50	0.023897952407466096	0.7209773805769221
>100	0.011948975656198084	0.9264043457684668
>500	3.620901713999394E-4	0.10279281096882945
>1k	7.241803427998788E-4	0.7262121737890451
>5k	3.620901713999394E-4	1.3245678573915523
>10k+	3.620901713999394E-4	2.3087138438894197
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14554	2.0948542641237857	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8350	1.2018711766822598	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3110	0.44764303706369196	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1468	0.2112990284274919	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01827995681899964	0.0
2	0.0	0.0	0.0	0.061460957178841306	0.0
3	0.0	0.0	0.0	0.11414177761784815	0.0
4	0.0	0.0	0.0	0.15041381792011516	0.0
5	0.0	0.0	0.0	0.27880532565671107	0.0
6	0.0	0.0	0.0	0.47383951061532925	0.0
7	0.0	0.0	0.0	0.646707448722562	0.0
8	0.0	0.0	0.0	0.9764663548038863	0.0
9	0.0	0.0	0.0	1.1933789132781576	0.0
10	0.0	0.0	0.0	1.468297948902483	0.0
11	0.0	0.0	0.0	1.7242173443684778	0.0
12	0.0	0.0	0.0	1.9126304426052536	0.0
13	0.0	0.0	0.0	2.024901043540842	0.0
14	0.0	0.0	0.0	2.0906801007556677	0.0
15	0.0	0.0	0.0	2.1560273479668948	0.0
16	0.0	0.0	0.0	2.2530406621086723	0.0
17	0.0	0.0	0.0	2.3753868297948904	0.0
18	0.0	0.0	0.0	2.520187117668226	0.0
19	0.0	0.0	0.0	2.62367758186398	0.0
20	0.0	0.0	0.0	2.7300467794170564	0.0
21	0.0	0.0	0.0	2.8587261604893848	0.0
22	0.0	0.0	0.0	2.995897804965815	0.0
23	0.0	0.0	0.0	3.1467434328895285	0.0
24	0.0	0.0	0.0	3.276718243972652	0.0
25	0.0	0.0	0.0	3.4041021950341848	0.0
26	0.0	0.0	0.0	3.520115149334293	0.0
27	0.0	0.0	0.0	3.6469233537243615	0.0
28	0.0	0.0	0.0	3.7774739114789493	0.0
29	0.0	0.0	0.0	3.922418136020151	0.0
30	0.0	0.0	0.0	4.100899604174163	0.0
31	0.0	0.0	0.0	4.26469953220583	0.0
32	0.0	0.0	0.0	4.4272040302267	0.0
33	0.0	0.0	0.0	4.58826916156891	0.0
34	0.0	0.0	0.0	4.753220582943505	0.0
35	0.0	0.0	0.0	4.919323497661029	0.0
36	0.0	0.0	0.0	5.079668945663908	0.0
37	0.0	0.0	0.0	5.247067290392227	0.0
38	1.439366678661389E-4	0.0	0.0	5.4471392587261604	0.0
39	1.439366678661389E-4	0.0	0.0	5.715437207628644	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAA	30	2.1642572E-6	45.000004	1
TCACGAC	45	3.8380676E-10	45.0	25
GCGATCG	20	7.0313574E-4	45.0	9
CGTTTTT	6255	0.0	44.10072	1
CGTTTTA	3125	0.0	43.92	1
CGTTATT	1440	0.0	43.90625	1
CGAAGGA	185	0.0	41.351353	4
GTTTTAT	3365	0.0	41.188705	2
CGGTTTT	635	0.0	40.74803	1
CACGACG	50	1.0804797E-9	40.500004	26
GCGCAAA	45	1.9264917E-8	40.0	1
AGGCGAT	285	0.0	39.473686	7
GGACGTA	40	3.4555887E-7	39.375	8
GTTATTT	1635	0.0	39.08257	2
ACAACGA	140	0.0	38.57143	13
TTGCACG	35	6.2447216E-6	38.57143	1
TACGTAG	35	6.2447216E-6	38.57143	2
CGGTTTA	210	0.0	38.571426	1
GGCACCG	120	0.0	37.500004	8
CGCGAAG	30	1.13945694E-4	37.500004	2
>>END_MODULE