FastQCFastQC Report
Sat 14 Jan 2017
SRR2934575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235087
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153711.2445277134323331No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90370.7316893465804433No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14800.11982961524167933No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAA207.033491E-445.01
CGTTTTA32700.044.174311
CGTTTTT56300.043.4413831
CGTTATT13000.042.057691
GCGATCA750.042.09
GTTTTAT37300.040.2949072
CGAGGTA356.248669E-638.5714265
ATAACGC356.248669E-638.57142611
AGGCGAT5400.038.3333327
CGGTCTA1050.036.4285731
TATGGGC2850.036.315794
TGCGATA250.002107169236.01
ATAACGG1000.036.02
TCACGAC1150.035.21739225
TATACGG1150.035.2173922
GTTATTT16100.034.5186352
CGGTTTT6250.034.1999971
CGCATCG401.5606609E-533.7521
TAAGGCG1000.033.755
TACGTAG401.5606609E-533.751
Produced by FastQC (version 0.11.5)