##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934575.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1235087
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.808565712374918	31.0	31.0	34.0	30.0	34.0
2	32.000882528923064	33.0	31.0	34.0	30.0	34.0
3	31.525502252068073	31.0	31.0	34.0	28.0	34.0
4	35.51858452076655	37.0	35.0	37.0	33.0	37.0
5	31.99577195776492	37.0	35.0	37.0	10.0	37.0
6	33.87910244379546	37.0	35.0	37.0	19.0	37.0
7	20.888020034216215	32.0	0.0	37.0	0.0	37.0
8	28.153244265383734	35.0	17.0	37.0	17.0	37.0
9	34.823129868584154	37.0	32.0	39.0	32.0	39.0
10	36.2507523761484	37.0	35.0	39.0	32.0	39.0
11	36.68755804246988	38.0	35.0	39.0	33.0	39.0
12	37.03251754734687	39.0	37.0	39.0	34.0	39.0
13	36.840510830411134	39.0	37.0	39.0	33.0	39.0
14	38.14344495569948	40.0	38.0	41.0	33.0	41.0
15	38.314651518476026	40.0	38.0	41.0	34.0	41.0
16	38.37590793199183	40.0	38.0	41.0	34.0	41.0
17	38.35637813368613	40.0	38.0	41.0	34.0	41.0
18	38.19669221682359	40.0	37.0	41.0	34.0	41.0
19	38.090142637725116	40.0	37.0	41.0	34.0	41.0
20	37.97526409070778	40.0	36.0	41.0	34.0	41.0
21	37.844946955153766	40.0	36.0	41.0	33.0	41.0
22	37.79383557595538	39.0	36.0	41.0	33.0	41.0
23	37.691271950882815	39.0	35.0	41.0	33.0	41.0
24	37.50162782055029	39.0	35.0	41.0	33.0	41.0
25	37.327455474796515	39.0	35.0	41.0	33.0	41.0
26	37.152976268068564	39.0	35.0	41.0	32.0	41.0
27	37.0664414733537	39.0	35.0	41.0	32.0	41.0
28	36.91429834497489	39.0	35.0	41.0	32.0	41.0
29	36.83148150697076	39.0	35.0	41.0	31.0	41.0
30	36.626568816609684	39.0	35.0	40.0	31.0	41.0
31	36.39836788825403	39.0	35.0	40.0	30.0	41.0
32	36.21833603624684	38.0	35.0	40.0	30.0	41.0
33	35.92439479971856	38.0	35.0	40.0	29.0	41.0
34	35.59313716361682	38.0	35.0	40.0	26.0	41.0
35	35.33682161661486	38.0	34.0	40.0	25.0	41.0
36	35.12915527408191	38.0	34.0	40.0	24.0	41.0
37	35.00770957835359	38.0	34.0	40.0	23.0	41.0
38	34.83223691934253	38.0	34.0	40.0	23.0	41.0
39	34.69698409909585	38.0	34.0	40.0	22.0	41.0
40	34.569031979123736	38.0	34.0	40.0	21.0	41.0
41	34.397774407794756	38.0	33.0	40.0	21.0	41.0
42	34.24753316972812	38.0	33.0	40.0	20.0	41.0
43	34.15346368312515	38.0	33.0	40.0	20.0	41.0
44	33.96777797839343	38.0	33.0	40.0	18.0	41.0
45	33.8724915734681	37.0	33.0	40.0	19.0	41.0
46	33.759506820167324	37.0	33.0	40.0	18.0	41.0
47	33.677918235719424	37.0	33.0	40.0	18.0	41.0
48	33.55694619083514	37.0	33.0	40.0	18.0	41.0
49	33.42451989212096	37.0	32.0	40.0	18.0	41.0
50	33.229685843993174	37.0	32.0	40.0	17.0	41.0
51	31.28167084585944	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	32.0
9	64.0
10	68.0
11	49.0
12	35.0
13	40.0
14	54.0
15	80.0
16	147.0
17	296.0
18	552.0
19	1074.0
20	1908.0
21	3271.0
22	5520.0
23	9063.0
24	14424.0
25	20330.0
26	25639.0
27	27690.0
28	27578.0
29	28532.0
30	31515.0
31	37331.0
32	47979.0
33	66750.0
34	88067.0
35	111161.0
36	138359.0
37	214473.0
38	205486.0
39	127506.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.638563113367724	18.898182881044008	23.326372959961525	20.136881045626744
2	37.09487671718673	22.039176187588406	24.59089926458622	16.275047830638652
3	29.126450201483784	21.671752678151417	33.133860205799266	16.067936914565532
4	25.752841702649288	24.09652113575805	32.752834415713224	17.39780274587944
5	20.710120015836942	35.340425411327296	28.08984306368701	15.859611509148749
6	24.639235940464115	31.823345238027766	30.208965036471113	13.328453785037006
7	45.654921475167335	42.24908852574758	9.350515388794474	2.745474610290611
8	79.43132750972198	3.5553770706031234	13.926144474033004	3.087150945641886
9	73.74743641541042	5.757084318756492	15.720269098452174	4.775210167380921
10	39.11222448297165	23.81969853135852	22.770703602256358	14.297373383413476
11	28.73028377758004	23.81986046327101	29.799358263830804	17.65049749531814
12	25.587023424260803	21.50334348916311	33.72134918430847	19.188283902267614
13	23.253503599341585	20.67473789295815	35.9474271852914	20.124331322408867
14	19.478627821359954	23.3000590241821	36.754171973310385	20.467141181147564
15	19.494416182827607	23.06185718091114	37.435014699369354	20.008711936891896
16	23.453003715527732	22.863085758331195	34.769453487891944	18.91445703824913
17	23.89588749618448	22.62885124691621	33.170618749934214	20.304642506965095
18	23.752415821719442	22.856932345656624	33.80255803842159	19.58809379420235
19	23.43721535406008	24.25173287387852	32.20898608761974	20.102065684441666
20	24.18639334718931	25.332790321653455	31.413414601562483	19.067401729594756
21	23.735412970908122	23.859614747787	33.63568720260192	18.769285078702957
22	23.698249596991953	21.91780821917808	33.58735052672403	20.796591657105935
23	21.941288346488953	23.118857214107184	33.672040916955645	21.26781352244822
24	21.152598966712468	23.216178293512925	34.38761803824346	21.243604701531147
25	22.82802749927738	24.026404617650414	32.43901036931002	20.70655751376219
26	21.386752512171206	24.450666228370956	32.17360396474094	21.98897729471689
27	20.868327494338455	23.797675791260048	33.70191735480982	21.63207935959167
28	20.44916673886131	23.737113255989254	33.217740936468445	22.59597906868099
29	21.595563713325458	23.315604487781023	33.47294563055072	21.615886168342797
30	21.996992924385083	22.713298739279093	33.35837880246493	21.931329533870894
31	22.142164883931255	22.989635547941155	33.09540137658319	21.7727981915444
32	23.019350053882846	22.682612641862477	32.56604595465745	21.731991349597234
33	22.58415803906931	22.972551731173592	32.604828647698504	21.838461582058592
34	20.59611994944486	23.575748105194208	33.96594733812274	21.862184607238195
35	20.812946780267303	24.481838121525044	32.8741214181673	21.831093680040354
36	22.147832500868358	24.81930422715161	31.203388911064568	21.829474360915466
37	21.6784728525197	24.992004611820867	30.8341841505902	22.495338385069232
38	21.46707074076563	25.13231861399238	31.315688692375517	22.08492195286648
39	21.43063606045566	24.44297446252774	30.48967400677037	23.636715470246227
40	22.76697916826912	23.791684310497967	31.49017032808215	21.951166193150765
41	21.07535744445533	24.369052544476624	31.1337581886944	23.421831822373644
42	21.373959891084596	24.753074074943708	30.97077371877447	22.90219231519723
43	21.63369867871656	24.3046036433061	30.9983831098538	23.06331456812354
44	21.90218178962292	24.474794083331783	30.826249486878254	22.79677464016705
45	21.10126655045353	24.474308287594315	30.502466627856982	23.921958534095168
46	21.52067020379941	24.536975937727465	30.470647007052943	23.471706851420183
47	21.004593198697744	24.479328176881467	31.78456254498671	22.73151607943408
48	21.28028227970985	24.207687393681578	31.351880474816753	23.160149851791818
49	21.656531078377476	24.021951490056974	31.40175550386329	22.919761927702258
50	20.48908295528979	24.53770463133366	32.154576965023516	22.81863544835303
51	20.617009166155906	24.22768598487394	31.403779652769398	23.751525196200753
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	807.0
1	1497.0
2	2187.0
3	22968.5
4	43750.0
5	29310.5
6	14871.0
7	12821.0
8	10771.0
9	10409.5
10	10048.0
11	9532.0
12	9016.0
13	8521.5
14	8027.0
15	7528.5
16	7030.0
17	6623.5
18	6217.0
19	6056.5
20	5896.0
21	6042.0
22	6188.0
23	6471.0
24	6754.0
25	7890.0
26	10367.5
27	11709.0
28	13341.5
29	14974.0
30	17064.0
31	19154.0
32	22560.0
33	25966.0
34	29382.0
35	32798.0
36	35797.5
37	38797.0
38	40954.5
39	43112.0
40	47115.5
41	51119.0
42	54978.0
43	58837.0
44	65400.5
45	71964.0
46	80170.0
47	88376.0
48	90537.5
49	92699.0
50	89814.5
51	86930.0
52	81076.5
53	75223.0
54	71413.0
55	67603.0
56	65662.5
57	63722.0
58	61268.5
59	58815.0
60	54845.5
61	50876.0
62	46237.5
63	41599.0
64	36744.5
65	31890.0
66	27725.0
67	23560.0
68	20328.5
69	17097.0
70	14120.0
71	11143.0
72	9052.0
73	6961.0
74	5562.5
75	3376.5
76	2589.0
77	1964.5
78	1340.0
79	1067.5
80	795.0
81	565.5
82	336.0
83	257.5
84	179.0
85	126.0
86	73.0
87	63.0
88	53.0
89	38.0
90	23.0
91	17.0
92	11.0
93	8.5
94	6.0
95	5.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1235087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.759942629040346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.72967055595927	26.367785744370277
2	7.658409266787738	5.6304537055365635
3	3.6131845138004723	3.984613663063271
4	2.4631053784241423	3.6217444960060874
5	1.9497443907122793	3.5836245971938303
6	1.7095437528323543	3.7705638165571003
7	1.4309232590932353	3.682045983757873
8	1.3223919762203133	3.88888425431696
9	1.177769507364834	3.8965265568867964
>10	6.906094894967809	37.517296749408565
>50	0.023731601394101312	0.5764067402243586
>100	0.013127783033736797	0.9002168540147724
>500	0.0016121835867735337	0.38809307129278753
>1k	2.3031194096764768E-4	0.1253005553039541
>5k	2.3031194096764768E-4	0.7650953501904278
>10k+	2.3031194096764768E-4	1.3013478618764043
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15371	1.2445277134323331	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9037	0.7316893465804433	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1480	0.11982961524167933	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.096595624437793E-5	0.0	0.0	0.03279121227897306	0.0
2	8.096595624437793E-5	0.0	0.0	0.13764212561544248	0.0
3	8.096595624437793E-5	0.0	0.0	0.29835954876053267	0.0
4	8.096595624437793E-5	0.0	0.0	0.3751962412364473	0.0
5	8.096595624437793E-5	0.0	0.0	0.6505614584235766	0.0
6	8.096595624437793E-5	0.0	0.0	1.117896957866126	0.0
7	8.096595624437793E-5	0.0	0.0	1.4929312671900845	0.0
8	1.6193191248875587E-4	0.0	0.0	2.290203038328474	0.0
9	1.6193191248875587E-4	0.0	0.0	2.8224732346790145	0.0
10	2.4289786873313377E-4	0.0	0.0	3.4844508929330487	0.0
11	2.4289786873313377E-4	0.0	0.0	4.091047837115928	0.0
12	2.4289786873313377E-4	0.0	0.0	4.511423081936738	0.0
13	2.4289786873313377E-4	0.0	0.0	4.753268393238695	0.0
14	2.4289786873313377E-4	0.0	0.0	4.942161969156829	0.0
15	2.4289786873313377E-4	0.0	0.0	5.096968877496079	0.0
16	2.4289786873313377E-4	0.0	0.0	5.300031495756979	0.0
17	2.4289786873313377E-4	0.0	0.0	5.528922254059835	0.0
18	2.4289786873313377E-4	0.002267046774842582	0.0	5.796514739447504	0.0
19	2.4289786873313377E-4	0.002267046774842582	0.0	5.986056043015593	0.0
20	2.4289786873313377E-4	0.002267046774842582	0.0	6.185637125157985	0.0
21	2.4289786873313377E-4	0.002267046774842582	0.0	6.4265108449850095	0.0
22	2.4289786873313377E-4	0.002267046774842582	0.0	6.680986845461089	0.0
23	2.4289786873313377E-4	0.002267046774842582	0.0	6.928418807743908	0.0
24	2.4289786873313377E-4	0.002267046774842582	0.0	7.151803881022147	0.0
25	2.4289786873313377E-4	0.002267046774842582	0.0	7.3542997375893355	0.0
26	2.4289786873313377E-4	0.002267046774842582	0.0	7.546674849625978	0.0
27	2.4289786873313377E-4	0.002267046774842582	0.0	7.7735414590227245	0.0
28	2.4289786873313377E-4	0.002267046774842582	0.0	7.979275953839689	0.0
29	2.4289786873313377E-4	0.002267046774842582	0.0	8.20565676749897	0.0
30	2.4289786873313377E-4	0.002267046774842582	0.0	8.477378516655103	0.0
31	2.4289786873313377E-4	0.002267046774842582	0.0	8.696958189989855	0.0
32	2.4289786873313377E-4	0.002267046774842582	0.0	8.934512305610859	0.0
33	3.2386382497751173E-4	0.002267046774842582	0.0	9.151905898127014	0.0
34	3.2386382497751173E-4	0.002267046774842582	0.0	9.376100630967697	0.0
35	3.2386382497751173E-4	0.002267046774842582	0.0	9.617945942269653	0.0
36	3.2386382497751173E-4	0.002267046774842582	0.0	9.841331015547892	0.0
37	3.2386382497751173E-4	0.002267046774842582	0.0	10.083905020456049	0.0
38	3.2386382497751173E-4	0.002267046774842582	0.0	10.37206285872979	0.0
39	3.2386382497751173E-4	0.002267046774842582	0.0	10.814460843649071	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCAA	20	7.033491E-4	45.0	1
CGTTTTA	3270	0.0	44.17431	1
CGTTTTT	5630	0.0	43.441383	1
CGTTATT	1300	0.0	42.05769	1
GCGATCA	75	0.0	42.0	9
GTTTTAT	3730	0.0	40.294907	2
CGAGGTA	35	6.248669E-6	38.571426	5
ATAACGC	35	6.248669E-6	38.571426	11
AGGCGAT	540	0.0	38.333332	7
CGGTCTA	105	0.0	36.42857	31
TATGGGC	285	0.0	36.31579	4
TGCGATA	25	0.0021071692	36.0	1
ATAACGG	100	0.0	36.0	2
TCACGAC	115	0.0	35.217392	25
TATACGG	115	0.0	35.217392	2
GTTATTT	1610	0.0	34.518635	2
CGGTTTT	625	0.0	34.199997	1
CGCATCG	40	1.5606609E-5	33.75	21
TAAGGCG	100	0.0	33.75	5
TACGTAG	40	1.5606609E-5	33.75	1
>>END_MODULE