##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934574.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	46678
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78013196795064	34.0	31.0	34.0	31.0	34.0
2	32.960516731650884	34.0	31.0	34.0	31.0	34.0
3	32.60289643943614	34.0	31.0	34.0	31.0	34.0
4	36.21249839324735	37.0	37.0	37.0	35.0	37.0
5	35.24799691503492	37.0	37.0	37.0	35.0	37.0
6	35.854642443977895	37.0	37.0	37.0	35.0	37.0
7	19.620806375594498	32.0	0.0	37.0	0.0	37.0
8	27.55619349586529	35.0	17.0	37.0	17.0	37.0
9	35.06774069154634	35.0	32.0	39.0	32.0	39.0
10	37.0192167616436	37.0	37.0	39.0	34.0	39.0
11	37.73465015639059	39.0	37.0	39.0	35.0	39.0
12	37.8546638673465	39.0	37.0	39.0	35.0	39.0
13	37.70673550709113	39.0	37.0	39.0	35.0	39.0
14	39.06489138352114	40.0	39.0	41.0	35.0	41.0
15	39.202707913792366	40.0	39.0	41.0	35.0	41.0
16	39.30101975234586	41.0	39.0	41.0	35.0	41.0
17	39.25990830798235	40.0	39.0	41.0	35.0	41.0
18	39.122391704871674	40.0	39.0	41.0	35.0	41.0
19	39.02684348086893	40.0	38.0	41.0	35.0	41.0
20	38.975641629889886	40.0	38.0	41.0	35.0	41.0
21	38.91882685633489	40.0	38.0	41.0	35.0	41.0
22	38.84517331505206	40.0	38.0	41.0	35.0	41.0
23	38.78885127897511	40.0	38.0	41.0	35.0	41.0
24	38.68014910664553	40.0	38.0	41.0	35.0	41.0
25	38.562106345601784	40.0	37.0	41.0	35.0	41.0
26	38.40239513261065	40.0	37.0	41.0	34.0	41.0
27	38.33478298127598	40.0	37.0	41.0	34.0	41.0
28	38.235635631346675	40.0	37.0	41.0	34.0	41.0
29	38.14053729808475	40.0	37.0	41.0	34.0	41.0
30	37.978340974334806	40.0	36.0	41.0	34.0	41.0
31	37.795492523244356	40.0	36.0	41.0	33.0	41.0
32	37.62007798106174	40.0	36.0	41.0	33.0	41.0
33	37.432923432880585	40.0	36.0	41.0	33.0	41.0
34	37.15030635417113	40.0	35.0	41.0	32.0	41.0
35	36.91105017352928	40.0	35.0	41.0	31.0	41.0
36	36.69968721881828	40.0	35.0	41.0	31.0	41.0
37	36.55814302240884	40.0	35.0	41.0	30.0	41.0
38	36.324071296970736	40.0	35.0	41.0	29.0	41.0
39	36.15733321907537	40.0	35.0	41.0	27.0	41.0
40	35.918998243283774	40.0	35.0	41.0	25.0	41.0
41	35.75650199237328	40.0	35.0	41.0	24.0	41.0
42	35.68441235699901	40.0	35.0	41.0	24.0	41.0
43	35.57401773854921	39.0	35.0	41.0	24.0	41.0
44	35.42148335404259	39.0	35.0	41.0	23.0	41.0
45	35.31972235314281	39.0	35.0	41.0	23.0	41.0
46	35.205364411500064	39.0	35.0	41.0	23.0	41.0
47	35.06116371738292	39.0	35.0	41.0	22.0	41.0
48	34.98935258580059	39.0	35.0	41.0	22.0	41.0
49	34.88047902652213	39.0	35.0	40.0	22.0	41.0
50	34.75386691803419	38.0	34.0	40.0	22.0	41.0
51	32.79808475084622	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	2.0
9	5.0
10	2.0
11	2.0
12	3.0
13	1.0
14	6.0
15	5.0
16	4.0
17	19.0
18	12.0
19	27.0
20	48.0
21	83.0
22	154.0
23	251.0
24	334.0
25	577.0
26	640.0
27	725.0
28	689.0
29	658.0
30	696.0
31	728.0
32	941.0
33	1609.0
34	2365.0
35	3167.0
36	3864.0
37	8463.0
38	11682.0
39	8910.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.71631175286002	20.03299198766014	25.54308239427567	15.707613865204165
2	34.45734607309653	24.654012596940746	24.49547966922319	16.393161660739537
3	30.67612151334676	23.342902437979347	29.424996786494706	16.555979262179186
4	27.186254766699513	24.99464415784738	29.33716097519174	18.481940100261365
5	24.369081794421355	31.070311495779595	26.862761900681264	17.697844809117786
6	26.87561592184755	33.38831997943357	26.601396803633403	13.134667295085478
7	43.333047688418525	48.08475084622306	6.662667637859377	1.919533827499036
8	82.81631603753374	5.182312866875187	9.601953811217276	2.399417284373795
9	75.99511547195681	7.817387205964266	11.455075196023824	4.732422126055101
10	40.357341788422815	30.275504520330777	17.580016281760145	11.787137409486267
11	33.39046231629462	23.771369810188954	26.86490423754231	15.973263635974119
12	30.5754316808775	21.335532799177344	29.547109987574444	18.54192553237071
13	25.74231972235314	23.617121556193496	31.323107245383262	19.317451476070097
14	20.81280260508162	25.17888512789751	32.784180984618025	21.224131282402844
15	21.322678778011053	24.08200865504092	35.196452290158106	19.39886027678992
16	25.498093320193664	23.26577831098162	31.98294699858606	19.253181370238657
17	25.234585886284762	23.435022923004414	30.472599511547195	20.857791679163633
18	25.528086036248336	23.171515489095505	31.269548823857065	20.030849650799095
19	25.515232015082052	25.082479969150352	28.694459916877328	20.707828098890268
20	26.24791122156048	25.393118814002314	29.763486010540298	18.59548395389691
21	25.498093320193664	24.628304554608167	31.479497836239773	18.394104288958395
22	25.20245083336904	22.40670122970136	31.048888127169118	21.341959809760485
23	22.67663567419341	25.33313338189297	32.34714426496422	19.6430866789494
24	23.259351300398475	23.40503020694974	32.3685676335747	20.967050859077084
25	23.270062984703717	25.50452033077681	30.429752774326236	20.795663910193237
26	21.8689746775783	26.194352800034277	29.787051716011824	22.149620806375594
27	20.99061656454861	25.641629889883887	31.16671665452676	22.201036891040747
28	19.61952097347787	25.832297870517163	32.60208235142894	21.94609880457603
29	22.12391276404302	25.069625947984058	31.7172972278161	21.089164060156822
30	22.417412914006597	24.65829727066284	31.670165816873048	21.254123998457516
31	24.02630789665367	24.878957967350786	30.592570375765888	20.502163760229656
32	25.84086721796135	24.634731565191313	29.324306954025452	20.200094262821885
33	24.12485539226188	24.780410471742577	29.840610137538025	21.254123998457516
34	22.365996829341444	25.684476627104846	31.59732636359741	20.352200179956295
35	21.88397103560564	26.26076524272677	31.466643815073482	20.388619906594112
36	23.48215433394747	27.145550366339606	29.268606195638203	20.103689104074725
37	22.003941899824326	28.169587385920565	29.23861347958353	20.58785723467158
38	23.533570418612623	27.475470242941004	29.24075581644458	19.7502035220018
39	22.721624748275417	26.39573246497279	28.861562192039074	22.021080594712714
40	25.30956767642144	25.18316980161961	28.27884656583401	21.22841595612494
41	21.048459659796904	25.320279360726683	29.686361883542567	23.944899095933845
42	21.691160718111316	25.086764642872446	30.189811045888852	23.032263593127382
43	21.905394404216118	24.752560092548954	30.41261407943785	22.929431423797077
44	22.295299712926862	25.12746904323236	29.669223188654183	22.9080080551866
45	21.526200779810615	26.59496979305026	28.439521830412613	23.439307596726508
46	21.712584086721794	27.66828056043532	27.66828056043532	22.950854792407558
47	20.73782081494494	26.275761600754105	31.203136381164576	21.783281203136383
48	21.778996529414286	26.762072068212007	30.23908479369296	21.219846608680747
49	22.34028878700887	26.479283602553664	30.02485110758816	21.155576502849307
50	21.586186211919962	26.809203479155062	29.907022580230514	21.697587728694458
51	21.14272248168302	26.61639316166074	28.998671751146148	23.24221260551009
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43.0
1	99.5
2	156.0
3	617.5
4	1079.0
5	715.0
6	351.0
7	292.0
8	233.0
9	241.0
10	249.0
11	235.5
12	222.0
13	216.5
14	211.0
15	204.5
16	198.0
17	200.5
18	203.0
19	199.0
20	195.0
21	220.0
22	245.0
23	262.0
24	279.0
25	357.5
26	500.5
27	565.0
28	627.0
29	689.0
30	830.5
31	972.0
32	1137.0
33	1302.0
34	1444.5
35	1587.0
36	1676.0
37	1765.0
38	1827.5
39	1890.0
40	2054.5
41	2219.0
42	2375.0
43	2531.0
44	2652.0
45	2773.0
46	2854.5
47	2936.0
48	2851.5
49	2767.0
50	2749.5
51	2732.0
52	2708.5
53	2685.0
54	2668.5
55	2652.0
56	2589.0
57	2526.0
58	2452.0
59	2378.0
60	2187.0
61	1996.0
62	1822.0
63	1648.0
64	1436.5
65	1225.0
66	1057.5
67	890.0
68	768.0
69	646.0
70	567.5
71	489.0
72	375.5
73	262.0
74	214.0
75	150.0
76	134.0
77	96.0
78	58.0
79	46.5
80	35.0
81	26.5
82	18.0
83	17.0
84	16.0
85	13.5
86	11.0
87	7.5
88	4.0
89	4.5
90	5.0
91	4.5
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	46678.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.49715069197481
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.47398490104061	56.941171429795624
2	15.568251377269945	22.88444234971507
3	4.6987495263357335	10.36034106002828
4	1.4632582271839567	4.301812416984447
5	0.4896959804121608	1.799562963280346
6	0.12533885212930304	0.5527229101503921
7	0.05829714052525723	0.29992716054672436
8	0.029148570262628615	0.1713869488838425
9	0.020403999183840033	0.13496722224602598
>10	0.05829714052525723	0.8740734393075967
>50	0.008744571078788585	0.36848194010026136
>100	0.005829714052525724	1.3111101589613952
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	378	0.8098033334761559	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	234	0.5013068254852393	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68	0.14567890655126614	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	53	0.11354385363554564	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	51	0.10925917991344959	No Hit
GATACTAAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	49	0.10497450619135353	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	49	0.10497450619135353	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.03641972663781653	0.0
3	0.0	0.0	0.0	0.04927374780410472	0.0
4	0.0	0.0	0.0	0.08783581130296927	0.0
5	0.0	0.0	0.0	0.24636873902052359	0.0
6	0.0	0.0	0.0	0.2956424868246283	0.0
7	0.0	0.0	0.0	0.37490895068340546	0.0
8	0.0	0.0	0.0	0.47774112001371094	0.0
9	0.0	0.0	0.0	0.5312995415399118	0.0
10	0.0	0.0	0.0	1.0583144093577275	0.0
11	0.0	0.0	0.0	1.169715926132225	0.0
12	0.0	0.0	0.0	1.508205150177814	0.0
13	0.0	0.0	0.0	1.576759929731351	0.0
14	0.0	0.0	0.0	1.6088949826470715	0.0
15	0.0	0.0	0.0	1.6346030249796477	0.0
16	0.0	0.0	0.0	1.711727151977377	0.0
17	0.0	0.0	0.0	1.7952782895582502	0.0
18	0.0	0.0	0.0	1.8938257851664595	0.0
19	0.0	0.0	0.0	1.9623805647199966	0.0
20	0.0	0.0	0.0	2.04593170230087	0.0
21	0.0	0.0	0.0	2.1037747975491667	0.0
22	0.0	0.0	0.0	2.174471913963752	0.0
23	0.0	0.0	0.0	2.283731093877201	0.0
24	0.0	0.0	0.0	2.356570547152834	0.0
25	0.0	0.0	0.0	2.4251253267063713	0.0
26	0.0	0.0	0.0	2.5086764642872446	0.0
27	0.0	0.0	0.0	2.6329320022280305	0.0
28	0.0	0.0	0.0	2.7229101503920474	0.0
29	0.0	0.0	0.0	2.8064612879729207	0.0
30	0.0	0.0	0.0	2.902866446720082	0.0
31	0.0	0.0	0.0	2.9842752474399075	0.0
32	0.0	0.0	0.0	3.0678263850207808	0.0
33	0.0	0.0	0.0	3.162089206906894	0.0
34	0.0	0.0	0.0	3.2670637130982474	0.0
35	0.0	0.0	0.0	3.357041861262265	0.0
36	0.0	0.0	0.0	3.4705857148978105	0.0
37	0.0	0.0	0.0	3.571275547367068	0.0
38	0.0	0.0	0.0	3.729808475084622	0.0
39	0.0	0.0	0.0	3.9397574874673293	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGAGG	30	2.1208907E-6	45.000004	3
CCTATGC	20	6.9638406E-4	45.0	35
TATACTG	20	6.9638406E-4	45.0	18
TACAGGA	20	6.9638406E-4	45.0	4
AGCTACG	20	6.9638406E-4	45.0	9
TGATTCA	20	6.9638406E-4	45.0	44
CGAAGGA	20	6.9638406E-4	45.0	4
CACGACG	20	6.9638406E-4	45.0	26
GCTACGA	20	6.9638406E-4	45.0	10
TCACGAC	20	6.9638406E-4	45.0	25
AACTAAC	20	6.9638406E-4	45.0	13
CGAATAT	20	6.9638406E-4	45.0	14
GTCTCAC	20	6.9638406E-4	45.0	22
AAAGGAT	20	6.9638406E-4	45.0	5
TACGAAT	20	6.9638406E-4	45.0	12
ACGGGAG	20	6.9638406E-4	45.0	5
CTAAAGC	20	6.9638406E-4	45.0	5
CGACGGT	20	6.9638406E-4	45.0	28
CTACGAA	20	6.9638406E-4	45.0	11
ACAAGCG	40	6.5756467E-9	45.0	14
>>END_MODULE