FastQCFastQC Report
Sat 14 Jan 2017
SRR2934573.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934573.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461107
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19030.4127024746967623No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11550.25048416094312165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT253.8871593E-545.028
GTCATAC207.028888E-445.039
CGACGGT207.028888E-445.028
TGTAGCG207.028888E-445.01
CGTTTAG253.8871593E-545.02
CGTTTTA2850.043.4210551
CGTTATT1150.041.086961
GCATTAG2150.039.7674416
AGGCGAT2450.039.4897967
TTGTACG403.4522236E-739.3751
CGTTTTT7550.039.0397341
CATTAGA2150.038.72093217
CGTATAG356.2401523E-638.5714262
CTAACGG356.2401523E-638.5714262
AATAGGC356.2401523E-638.5714264
TAGGCGA659.094947E-1238.076926
ACGCCTC301.13886206E-437.49999622
ATAGGAT1950.036.9230775
TGCATTA2400.036.562515
TTGACCC2350.036.382988
Produced by FastQC (version 0.11.5)