##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934573.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	461107
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09280058641487	33.0	31.0	34.0	30.0	34.0
2	32.29494021994895	33.0	31.0	34.0	30.0	34.0
3	32.13410770168312	33.0	31.0	34.0	30.0	34.0
4	35.90746182556327	37.0	35.0	37.0	35.0	37.0
5	32.159832750316085	37.0	35.0	37.0	0.0	37.0
6	34.0413353082907	37.0	35.0	37.0	19.0	37.0
7	20.561156087415718	32.0	0.0	37.0	0.0	37.0
8	27.99786817376444	35.0	17.0	37.0	17.0	37.0
9	34.90775459925787	37.0	32.0	39.0	32.0	39.0
10	36.35087517647748	37.0	35.0	39.0	32.0	39.0
11	36.76912950790164	38.0	37.0	39.0	33.0	39.0
12	37.09550928526351	39.0	37.0	39.0	34.0	39.0
13	36.89035950441004	39.0	37.0	39.0	33.0	39.0
14	38.267608169036684	40.0	38.0	41.0	34.0	41.0
15	38.45425898977894	40.0	38.0	41.0	34.0	41.0
16	38.53724840438336	40.0	38.0	41.0	34.0	41.0
17	38.51526869034736	40.0	38.0	41.0	34.0	41.0
18	38.40128863799509	40.0	38.0	41.0	34.0	41.0
19	38.34766117191888	40.0	37.0	41.0	34.0	41.0
20	38.31037481538992	40.0	37.0	41.0	34.0	41.0
21	38.231688089749234	40.0	37.0	41.0	34.0	41.0
22	38.211642850791684	40.0	37.0	41.0	34.0	41.0
23	38.13885063553579	40.0	37.0	41.0	34.0	41.0
24	38.025672999976145	40.0	36.0	41.0	34.0	41.0
25	37.87258488810623	40.0	36.0	41.0	33.0	41.0
26	37.74152203284704	40.0	36.0	41.0	33.0	41.0
27	37.69420980379825	40.0	36.0	41.0	33.0	41.0
28	37.606210705974966	40.0	36.0	41.0	33.0	41.0
29	37.5756191079294	40.0	36.0	41.0	33.0	41.0
30	37.41251162962176	40.0	36.0	41.0	33.0	41.0
31	37.29523299364356	40.0	35.0	41.0	32.0	41.0
32	37.21457709381987	40.0	35.0	41.0	32.0	41.0
33	37.03813865328438	40.0	35.0	41.0	31.0	41.0
34	36.87426562598269	39.0	35.0	41.0	31.0	41.0
35	36.70915427438751	39.0	35.0	41.0	31.0	41.0
36	36.538180942818045	39.0	35.0	41.0	30.0	41.0
37	36.480495850203965	39.0	35.0	41.0	30.0	41.0
38	36.343698967918506	39.0	35.0	41.0	30.0	41.0
39	36.255404927706586	39.0	35.0	41.0	30.0	41.0
40	36.136606037210456	39.0	35.0	41.0	29.0	41.0
41	35.99927782488663	39.0	35.0	40.0	29.0	41.0
42	35.876085160277334	39.0	35.0	40.0	28.0	41.0
43	35.79905748557276	39.0	35.0	40.0	28.0	41.0
44	35.664982314300154	38.0	35.0	40.0	27.0	41.0
45	35.58046830778973	38.0	35.0	40.0	27.0	41.0
46	35.46559692218943	38.0	34.0	40.0	27.0	41.0
47	35.43277156928869	38.0	34.0	40.0	27.0	41.0
48	35.31647318301392	38.0	34.0	40.0	26.0	41.0
49	35.20842884623309	38.0	34.0	40.0	26.0	41.0
50	35.05410674745775	38.0	34.0	40.0	26.0	41.0
51	33.277278375734916	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	23.0
9	21.0
10	22.0
11	19.0
12	15.0
13	15.0
14	23.0
15	39.0
16	55.0
17	97.0
18	155.0
19	291.0
20	499.0
21	800.0
22	1378.0
23	2121.0
24	3034.0
25	4292.0
26	5078.0
27	5625.0
28	6170.0
29	7051.0
30	8552.0
31	10985.0
32	15201.0
33	22684.0
34	31719.0
35	41311.0
36	54072.0
37	89719.0
38	91706.0
39	58322.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.28804594161442	19.97952752831772	25.977267749134146	13.75515878093371
2	31.728644327672317	23.62943958777464	27.462389423712935	17.179526660840107
3	31.685270446989527	23.14148343009323	29.28842980045846	15.884816322458779
4	27.223833079957583	26.317752712494062	28.17003428705268	18.288379920495675
5	22.51342963780641	37.541828686183464	23.600162218313752	16.34457945769637
6	26.675587228127096	35.09424059925353	24.827209302829928	13.402962869789443
7	49.162558798717	43.55106298538083	4.803874155022587	2.4825040608795788
8	87.16545183655853	3.742515294714676	6.878013129273683	2.2140197394530987
9	82.19957623718574	6.031571847749004	8.408243639762572	3.3606082753026953
10	43.55995463092081	28.458470593593244	15.423318231993877	12.558256543492075
11	32.30660128777052	26.402331779825506	24.196553077702138	17.094513854701837
12	29.316405953498865	23.256207344499217	27.996321894918097	19.43106480708382
13	25.470227083952313	24.218240018043534	29.21859785255917	21.09293504544498
14	22.17034224160553	25.38890105767208	29.901953342716546	22.538803358005843
15	21.320864788433052	25.18612816548003	32.82470229252647	20.668304753560456
16	25.87815843177397	23.39999175896267	29.711542006519092	21.010307802744265
17	26.156401876354078	22.923963418469032	28.54109783629396	22.37853686888293
18	25.82676038316486	24.194167514264585	29.417683964893182	20.561388137677373
19	25.595143860318753	25.810928916715643	27.202796747826426	21.391130475139175
20	26.51401952258369	25.511866009407797	27.790946569885083	20.18316789812343
21	26.275246309424926	25.834567681687766	28.12232301830161	19.767862990585698
22	26.857540657591407	22.613189563376828	29.038162508918752	21.491107270113012
23	24.23558957031665	24.820486351324096	29.190838568922178	21.753085509437074
24	23.801417024681907	24.40388022736588	30.75511757574706	21.03958517220515
25	23.977298110850626	25.677554233616057	28.470398410781012	21.874749244752305
26	23.621849158655152	25.810495177908816	27.69942768164439	22.868227981791645
27	22.344271503143524	26.148377708427763	28.81175085175458	22.695599936674135
28	21.745711949721	26.23100495112848	29.26045364741806	22.76282945173246
29	23.183989833162368	25.107404572040764	29.240067923497147	22.46853767129972
30	23.126953180064497	25.890086248961737	29.674891077342135	21.308069493631628
31	24.821570698341166	26.195221499565175	27.806994905737714	21.176212896355942
32	25.612276543188457	25.405383132331544	27.525932158913225	21.456408165566778
33	25.053187221187272	25.632011658899128	27.590776110533998	21.724025009379602
34	23.423413654531377	25.765603211402127	29.02211417306612	21.788868961000375
35	23.759561229823014	26.734792575259103	28.366951705352555	21.138694489565328
36	24.6775694144743	27.34441246825574	27.00631306833338	20.97170504893658
37	23.602330912347895	28.09217817122707	26.92628825847363	21.379202657951407
38	23.676283378912053	27.41597937138235	27.373689837716626	21.534047411988976
39	23.941297789883908	26.68122583261586	27.218194475468817	22.159281902031417
40	25.13017585939923	25.359406818807784	27.591860457551064	21.91855686424192
41	22.660033354514244	25.61618019244991	28.337891205295087	23.385895247740763
42	22.685190205310267	25.13342890045044	27.935815331365603	24.245565562873693
43	22.820082974233745	26.160088656212118	28.129262839210856	22.89056553034328
44	22.418440839111096	25.400828874859847	28.338108074698496	23.842622211330557
45	22.604731656643686	26.823709030658826	27.44959412891151	23.121965183785974
46	22.669792477667876	26.7781664559419	27.490582446156747	23.061458620233484
47	22.112438110894	26.224281999622644	29.47146757693984	22.19181231254351
48	22.280945637346647	25.87056800265448	29.753831540184816	22.094654819814057
49	23.251869956430937	25.635915308160577	29.147247818835975	21.96496691657251
50	22.02634095773866	26.386066574569462	29.488817129212958	22.09877533847892
51	21.805784774466662	26.301704376641432	28.292565499981563	23.59994534891034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	272.0
1	702.5
2	1133.0
3	3530.0
4	5927.0
5	3986.5
6	2046.0
7	1802.5
8	1559.0
9	1519.5
10	1480.0
11	1457.0
12	1434.0
13	1411.5
14	1389.0
15	1369.0
16	1349.0
17	1293.0
18	1237.0
19	1300.5
20	1364.0
21	1546.5
22	1729.0
23	2010.5
24	2292.0
25	2871.5
26	4168.5
27	4886.0
28	5897.0
29	6908.0
30	8163.5
31	9419.0
32	10377.0
33	11335.0
34	13066.0
35	14797.0
36	15914.0
37	17031.0
38	18302.0
39	19573.0
40	21016.0
41	22459.0
42	23635.0
43	24811.0
44	25959.5
45	27108.0
46	28049.5
47	28991.0
48	29459.5
49	29928.0
50	29941.0
51	29954.0
52	29388.0
53	28822.0
54	27753.5
55	26685.0
56	25997.0
57	25309.0
58	24689.0
59	24069.0
60	22674.0
61	21279.0
62	19647.5
63	18016.0
64	16147.5
65	14279.0
66	12158.0
67	10037.0
68	8465.0
69	6893.0
70	5755.5
71	4618.0
72	3795.5
73	2973.0
74	2402.5
75	1471.5
76	1111.0
77	859.5
78	608.0
79	457.0
80	306.0
81	239.5
82	173.0
83	142.5
84	112.0
85	82.5
86	53.0
87	47.5
88	42.0
89	26.0
90	10.0
91	10.5
92	11.0
93	7.5
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	461107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.51755424460355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.41946096166099	21.890846839076506
2	8.90732753711869	6.149183228738908
3	5.287681125446951	5.475534602273445
4	3.9709371083283194	5.482681481545278
5	3.0496032663518577	5.263242308541021
6	2.704575222090459	5.601319316227088
7	2.1818958825545676	5.271959663750811
8	1.9055708670323044	5.262051661577904
9	1.5759740562223055	4.895889297636717
>10	6.975911859998964	33.23013411103694
>50	0.014041408796392041	0.32495261702507755
>100	0.0057442126894331055	0.47850649774811405
>500	0.0	0.0
>1k	0.0012764917087629123	0.6736983748221607
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1903	0.4127024746967623	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1155	0.25048416094312165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0075904291194885355	0.0
2	0.0	0.0	0.0	0.03491597394964726	0.0
3	0.0	0.0	0.0	0.048795615768140586	0.0
4	0.0	0.0	0.0	0.06809699267198285	0.0
5	0.0	0.0	0.0	0.13033851145178885	0.0
6	0.0	0.0	0.0	0.17674856378237588	0.0
7	0.0	0.0	0.0	0.22814661239148398	0.0
8	0.0	0.0	0.0	0.35631642980913325	0.0
9	2.1686940341395815E-4	0.0	0.0	0.41378682171383213	0.0
10	2.1686940341395815E-4	0.0	0.0	0.5671134899275005	0.0
11	2.1686940341395815E-4	0.0	0.0	0.7158859006694759	0.0
12	2.1686940341395815E-4	0.0	0.0	0.8642245726046233	0.0
13	2.1686940341395815E-4	0.0	0.0	0.9255986137707733	0.0
14	2.1686940341395815E-4	0.0	0.0	0.9516229421804484	0.0
15	2.1686940341395815E-4	0.0	0.0	1.0088764646817332	0.0
16	2.1686940341395815E-4	0.0	0.0	1.0889012745414839	0.0
17	2.1686940341395815E-4	0.0	0.0	1.1819382486060719	0.0
18	2.1686940341395815E-4	0.0	0.0	1.2866861704550137	0.0
19	2.1686940341395815E-4	0.0	0.0	1.352831338496271	0.0
20	4.337388068279163E-4	0.0	0.0	1.430687454321882	0.0
21	4.337388068279163E-4	0.0	0.0	1.5102785253748046	0.0
22	4.337388068279163E-4	0.0	0.0	1.6102553203486392	0.0
23	4.337388068279163E-4	0.0	0.0	1.7271479287887628	0.0
24	4.337388068279163E-4	0.0	0.0	1.808473955068997	0.0
25	4.337388068279163E-4	0.0	0.0	1.9038964925711386	0.0
26	4.337388068279163E-4	0.0	0.0	1.9871743434820985	0.0
27	4.337388068279163E-4	0.0	0.0	2.077175145898891	0.0
28	4.337388068279163E-4	0.0	0.0	2.1678265565259256	0.0
29	4.337388068279163E-4	0.0	0.0	2.2530562320676113	0.0
30	4.337388068279163E-4	0.0	0.0	2.3649608442292136	0.0
31	4.337388068279163E-4	0.0	0.0	2.472961807129365	0.0
32	6.506082102418745E-4	0.0	0.0	2.589854415569488	0.0
33	6.506082102418745E-4	0.0	0.0	2.6928673821911184	0.0
34	6.506082102418745E-4	0.0	0.0	2.813446770489279	0.0
35	6.506082102418745E-4	0.0	0.0	2.927303207281607	0.0
36	6.506082102418745E-4	0.0	0.0	3.04636450975587	0.0
37	6.506082102418745E-4	0.0	0.0	3.1678113756676867	0.0
38	6.506082102418745E-4	0.0	0.0	3.3189693498472157	0.0
39	6.506082102418745E-4	0.0	0.0	3.5464653540284576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGT	25	3.8871593E-5	45.0	28
GTCATAC	20	7.028888E-4	45.0	39
CGACGGT	20	7.028888E-4	45.0	28
TGTAGCG	20	7.028888E-4	45.0	1
CGTTTAG	25	3.8871593E-5	45.0	2
CGTTTTA	285	0.0	43.421055	1
CGTTATT	115	0.0	41.08696	1
GCATTAG	215	0.0	39.76744	16
AGGCGAT	245	0.0	39.489796	7
TTGTACG	40	3.4522236E-7	39.375	1
CGTTTTT	755	0.0	39.039734	1
CATTAGA	215	0.0	38.720932	17
CGTATAG	35	6.2401523E-6	38.571426	2
CTAACGG	35	6.2401523E-6	38.571426	2
AATAGGC	35	6.2401523E-6	38.571426	4
TAGGCGA	65	9.094947E-12	38.07692	6
ACGCCTC	30	1.13886206E-4	37.499996	22
ATAGGAT	195	0.0	36.923077	5
TGCATTA	240	0.0	36.5625	15
TTGACCC	235	0.0	36.38298	8
>>END_MODULE