##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934572.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 255649 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.774206040313086 31.0 31.0 34.0 30.0 34.0 2 31.962362457901264 33.0 31.0 34.0 30.0 34.0 3 31.704716232021248 33.0 31.0 34.0 30.0 34.0 4 35.5697381957293 37.0 35.0 37.0 33.0 37.0 5 31.879256324100623 37.0 35.0 37.0 0.0 37.0 6 33.82194336766426 37.0 35.0 37.0 18.0 37.0 7 21.014077895865032 32.0 0.0 37.0 0.0 37.0 8 28.25764231426683 35.0 17.0 37.0 17.0 37.0 9 34.89273183153464 37.0 32.0 39.0 32.0 39.0 10 36.220517193495766 37.0 35.0 39.0 32.0 39.0 11 36.61755375534424 38.0 35.0 39.0 32.0 39.0 12 36.94270269001639 39.0 37.0 39.0 33.0 39.0 13 36.62299872090249 39.0 35.0 39.0 33.0 39.0 14 37.94474846371392 40.0 37.0 41.0 33.0 41.0 15 38.17100790537025 40.0 37.0 41.0 33.0 41.0 16 38.25705166067538 40.0 37.0 41.0 34.0 41.0 17 38.246556802490915 40.0 37.0 41.0 34.0 41.0 18 38.140978450922944 40.0 37.0 41.0 34.0 41.0 19 38.075634952610805 40.0 37.0 41.0 34.0 41.0 20 38.01204385700707 40.0 36.0 41.0 34.0 41.0 21 37.895235263975216 40.0 36.0 41.0 33.0 41.0 22 37.88859725639451 39.0 36.0 41.0 33.0 41.0 23 37.8110417017082 39.0 36.0 41.0 33.0 41.0 24 37.65504656775501 39.0 35.0 41.0 33.0 41.0 25 37.53800718954504 39.0 35.0 41.0 33.0 41.0 26 37.39107917496255 39.0 35.0 41.0 33.0 41.0 27 37.33683683487907 39.0 35.0 41.0 33.0 41.0 28 37.27364863543374 39.0 35.0 41.0 33.0 41.0 29 37.32248121447766 39.0 35.0 41.0 33.0 41.0 30 37.15254117950784 39.0 35.0 41.0 32.0 41.0 31 36.973217184499056 39.0 35.0 41.0 32.0 41.0 32 36.835837417709435 39.0 35.0 41.0 31.0 41.0 33 36.643276523671126 39.0 35.0 41.0 31.0 41.0 34 36.438347108731115 39.0 35.0 40.0 30.0 41.0 35 36.253449064928866 39.0 35.0 40.0 30.0 41.0 36 36.01360459066924 39.0 35.0 40.0 29.0 41.0 37 35.87497310765933 39.0 35.0 40.0 28.0 41.0 38 35.77414736611526 39.0 35.0 40.0 27.0 41.0 39 35.73070107843176 39.0 35.0 40.0 27.0 41.0 40 35.63101361632551 39.0 35.0 40.0 26.0 41.0 41 35.5799514177642 39.0 35.0 40.0 26.0 41.0 42 35.446217274466164 38.0 35.0 40.0 26.0 41.0 43 35.312408810517546 38.0 35.0 40.0 25.0 41.0 44 35.122840300568356 38.0 34.0 40.0 24.0 41.0 45 35.02658723484152 38.0 34.0 40.0 24.0 41.0 46 34.9224757382192 38.0 34.0 40.0 23.0 41.0 47 34.87826668596396 38.0 34.0 40.0 23.0 41.0 48 34.74820163583664 38.0 34.0 40.0 23.0 41.0 49 34.62137931304249 38.0 34.0 40.0 23.0 41.0 50 34.419090237004646 38.0 34.0 40.0 22.0 41.0 51 32.5466831475969 35.0 31.0 39.0 18.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 8.0 10 7.0 11 4.0 12 3.0 13 7.0 14 4.0 15 9.0 16 24.0 17 36.0 18 86.0 19 153.0 20 284.0 21 501.0 22 892.0 23 1434.0 24 2224.0 25 2922.0 26 3809.0 27 3984.0 28 4288.0 29 4754.0 30 5669.0 31 7132.0 32 9408.0 33 13787.0 34 18922.0 35 24496.0 36 28472.0 37 46073.0 38 46719.0 39 29529.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.92777597408948 20.504676333566728 27.567485106532786 16.000062585811015 2 32.0067749140423 24.61343482665686 27.17162985186721 16.20816040743363 3 29.15951167420956 23.30930298964596 31.213499759435788 16.31768557670869 4 26.81254376117255 26.04782338284132 29.801798559744025 17.337834296242114 5 20.69712770243576 36.92171688526065 25.841681367812903 16.53947404449069 6 25.30187874781439 33.31872997742999 27.52563084541696 13.853760429338664 7 48.574021412170595 42.07057332514502 6.854319790024604 2.5010854726597795 8 83.50355370058166 4.187773079495715 9.526733920336087 2.781939299586542 9 77.30247331301902 7.4950420302837095 11.398440830983105 3.804043825714163 10 36.73552409749305 35.89296261671276 16.99478581961987 10.376727466174325 11 29.762291266541236 24.647857022714735 29.953960312772594 15.635891397971438 12 27.49159981067792 22.848906117371865 31.917198972028054 17.74229509992216 13 21.995783280982913 27.983289588459176 33.027314794894565 16.993612335663354 14 17.928096726370924 30.712030948683545 32.692089544649114 18.66778278029642 15 17.41724004396653 26.76990717741904 38.07838090506906 17.734471873545367 16 20.478859686523318 25.917566663667763 35.51197149216308 18.091602157645834 17 20.14676372682858 25.06913776310488 31.727485732390896 23.056612777675642 18 20.868065198768623 24.681888057453776 34.955740096773305 19.494306647004294 19 22.329835047271846 26.44446096014457 32.31735700120086 18.908346991382714 20 24.108445564035065 25.99892821798638 31.507261909884253 18.385364308094303 21 21.56863512081017 26.687372139143907 32.73746425763449 19.00652848241143 22 21.88625811170785 23.684817855731882 31.96100903973808 22.46791499282219 23 19.389084252236465 26.86730634581008 31.93636587665119 21.80724352530227 24 19.437588255772564 25.11607712136562 35.59294188516286 19.853392737698954 25 19.0299981615418 27.134860687896296 33.134101834937745 20.701039315624158 26 18.823464985194544 29.009696889094034 32.01850975360748 20.148328372103936 27 18.51914147913741 29.21779471071665 33.37505720734288 18.888006602803063 28 17.04876608161972 28.078732950256015 34.78793189099116 20.0845690771331 29 18.907173507426197 25.986802217102355 33.98722467132671 21.118799604144744 30 19.606178784192387 26.472624575101015 33.441163470226755 20.48003317047984 31 22.345872661344266 28.53052427351564 30.635754491509843 18.48784857363025 32 22.520721770865524 28.661172154008035 29.971953733439204 18.846152341687237 33 22.315362078474784 28.19295205535715 29.046466053064947 20.445219813103122 34 19.563151039120044 28.301694901994534 31.341409510696305 20.793744548189117 35 20.097477400654803 28.37327742334216 30.611893651060633 20.9173515249424 36 23.020234775023567 27.155201076475947 30.354509503264243 19.470054645236242 37 20.33530348250922 29.452882663339185 30.934601739103222 19.277212115048368 38 19.1864626890776 30.869668960175865 29.76268242786007 20.181185922886456 39 20.31261612601653 28.2488881239512 30.62167268403162 20.81682306600065 40 21.222457353637214 27.008124420592296 30.793001341683322 20.976416884087165 41 19.821708670872955 26.456978122347437 30.485548545075474 23.235764661704135 42 21.816631396954417 26.21054649147855 29.415722338049434 22.557099773517596 43 21.83853643080943 26.356840824724525 29.748209459063013 22.056413285403035 44 19.86434525462646 28.971363079847762 29.349224913846722 21.815066751679062 45 19.40238373707701 29.63007874077348 28.354892841356705 22.61264468079281 46 20.868456360087464 28.990921145789734 29.396164272107463 20.744458222015343 47 20.176100825741543 27.38833322250429 30.987017355827717 21.448548595926447 48 20.929868687145266 26.9240247370418 31.10788620334912 21.038220372463808 49 21.11997308810126 25.4039718520315 31.929324972912077 21.54673008695516 50 20.114297337364903 27.62498582040219 30.732371337263203 21.528345504969703 51 19.691451951699403 27.378945350852145 29.052333472847536 23.877269224600916 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 383.0 1 556.0 2 729.0 3 2946.5 4 5164.0 5 3580.0 6 1996.0 7 1816.5 8 1637.0 9 1653.0 10 1669.0 11 1639.0 12 1609.0 13 1525.0 14 1441.0 15 1399.0 16 1357.0 17 1354.0 18 1351.0 19 1274.0 20 1197.0 21 1238.0 22 1279.0 23 1443.5 24 1608.0 25 1918.5 26 2700.5 27 3172.0 28 3668.0 29 4164.0 30 5085.5 31 6007.0 32 7151.0 33 8295.0 34 8755.0 35 9215.0 36 9932.5 37 10650.0 38 11616.5 39 12583.0 40 13946.0 41 15309.0 42 17080.5 43 18852.0 44 19656.0 45 20460.0 46 21719.0 47 22978.0 48 22398.0 49 21818.0 50 20177.0 51 18536.0 52 16725.0 53 14914.0 54 13313.0 55 11712.0 56 10431.5 57 9151.0 58 8166.5 59 7182.0 60 6345.0 61 5508.0 62 4786.0 63 4064.0 64 3406.0 65 2748.0 66 2261.0 67 1774.0 68 1438.0 69 1102.0 70 924.0 71 746.0 72 602.0 73 458.0 74 372.0 75 225.5 76 165.0 77 122.0 78 79.0 79 55.0 80 31.0 81 22.0 82 13.0 83 10.5 84 8.0 85 6.0 86 4.0 87 6.5 88 9.0 89 6.0 90 3.0 91 2.5 92 2.0 93 2.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 255649.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.31100311522592 #Duplication Level Percentage of deduplicated Percentage of total 1 72.7102042504298 29.31021270047789 2 9.01790363095911 7.270414827207997 3 3.909782830164606 4.728218035398668 4 2.4044536535690364 3.8770375487775115 5 1.811742980454794 3.6516588464550948 6 1.4469897227298767 3.4997764332398376 7 1.2321392646292142 3.4768138814402305 8 0.9943053758396423 3.206515768236616 9 0.8444100906790599 3.0635116016302604 >10 5.594091936550207 35.344182492622636 >50 0.019986037643910833 0.5119951669480546 >100 0.011991622586346498 0.8717211182341386 >500 0.0 0.0 >1k 0.0019986037643910833 1.187941579331088 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1888 0.7385125699689808 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1061 0.4150221592887122 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 446 0.17445794820241817 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06415045628967843 0.0 2 0.0 0.0 0.0 0.30901744188320707 0.0 3 0.0 0.0 0.0 0.41384867533219377 0.0 4 0.0 0.0 0.0 0.530805909665205 0.0 5 0.0 0.0 0.0 0.8507758684759181 0.0 6 0.0 0.0 0.0 1.0620029806492495 0.0 7 0.0 0.0 0.0 1.288876545576161 0.0 8 0.0 0.0 0.0 1.9468098838642045 0.0 9 0.0 0.0 0.0 2.180724352530227 0.0 10 0.0 0.0 0.0 2.7541668459489377 0.0 11 0.0 0.0 0.0 3.579517228700288 0.0 12 0.0 0.0 0.0 4.206939984118851 0.0 13 0.0 0.0 0.0 4.481926391263021 0.0 14 0.0 0.0 0.0 4.583628334161292 0.0 15 0.0 0.0 0.0 4.770603444566573 0.0 16 0.0 0.0 0.0 5.2118334122175325 0.0 17 0.0 0.0 0.0 5.8032693263028605 0.0 18 0.0 0.0 0.0 6.4584645353590275 0.0 19 0.0 0.0 0.0 6.861751855082555 0.0 20 0.0 0.0 0.0 7.229834656110526 0.0 21 0.0 0.0 0.0 7.716048175428028 0.0 22 0.0 0.0 0.0 8.248418730368591 0.0 23 0.0 0.0 0.0 8.787830189048265 0.0 24 0.0 0.0 0.0 9.226713188786187 0.0 25 0.0 0.0 0.0 9.58658160211853 0.0 26 0.0 0.0 0.0 9.953882080508823 0.0 27 0.0 0.0 0.0 10.27033158744998 0.0 28 0.0 0.0 0.0 10.63254696869536 0.0 29 0.0 0.0 0.0 11.039745901607281 0.0 30 0.0 0.0 0.0 11.508357161577006 0.0 31 0.0 0.0 0.0 11.948804806590285 0.0 32 0.0 0.0 0.0 12.334881028284874 0.0 33 0.0 0.0 0.0 12.722521895254822 0.0 34 0.0 0.0 0.0 13.15631979784783 0.0 35 0.0 0.0 0.0 13.66052673783195 0.0 36 0.0 0.0 0.0 14.10801528658434 0.0 37 0.0 0.0 0.0 14.535554608075916 0.0 38 0.0 0.0 0.0 14.968570188031245 0.0 39 0.0 0.0 0.0 15.439919577232846 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAAAAT 20 7.0229836E-4 45.000004 41 CGACGAA 20 7.0229836E-4 45.000004 38 CGGTTTA 40 6.7793735E-9 45.000004 1 TGTATAC 20 7.0229836E-4 45.000004 25 GACCAAT 20 7.0229836E-4 45.000004 9 CACTAGG 30 2.1588567E-6 45.000004 3 TCGAGAT 20 7.0229836E-4 45.000004 17 CGACTAG 20 7.0229836E-4 45.000004 2 TATACGG 20 7.0229836E-4 45.000004 2 CTCTCGA 20 7.0229836E-4 45.000004 14 CGTCAGT 20 7.0229836E-4 45.000004 20 TGGTCTC 20 7.0229836E-4 45.000004 19 TCAACGG 20 7.0229836E-4 45.000004 2 AGTCGTG 20 7.0229836E-4 45.000004 33 TGCCGAA 20 7.0229836E-4 45.000004 36 GCATATC 20 7.0229836E-4 45.000004 28 AACGTGG 25 3.8822676E-5 45.0 2 TACGGGA 105 0.0 45.0 4 CGTCAGG 25 3.8822676E-5 45.0 3 CGTTTTA 335 0.0 43.656715 1 >>END_MODULE