##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934571.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316763
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.053702610469024	33.0	31.0	34.0	30.0	34.0
2	32.24333018692209	33.0	31.0	34.0	30.0	34.0
3	32.12426325044276	33.0	31.0	34.0	30.0	34.0
4	35.87385521667619	37.0	35.0	37.0	35.0	37.0
5	32.1440004040876	37.0	35.0	37.0	0.0	37.0
6	34.01791875945107	37.0	35.0	37.0	19.0	37.0
7	20.71965160072357	32.0	0.0	37.0	0.0	37.0
8	28.0621916069743	35.0	17.0	37.0	17.0	37.0
9	34.8548630995413	37.0	32.0	39.0	32.0	39.0
10	36.333631137475024	37.0	35.0	39.0	32.0	39.0
11	36.763132057721386	39.0	37.0	39.0	33.0	39.0
12	37.080987994178614	39.0	37.0	39.0	34.0	39.0
13	36.84968888411841	39.0	37.0	39.0	33.0	39.0
14	38.25774790616328	40.0	38.0	41.0	34.0	41.0
15	38.43532862108264	40.0	38.0	41.0	34.0	41.0
16	38.50876207132777	40.0	38.0	41.0	34.0	41.0
17	38.49751707112257	40.0	38.0	41.0	34.0	41.0
18	38.3990238758946	40.0	38.0	41.0	34.0	41.0
19	38.36499528038313	40.0	37.0	41.0	34.0	41.0
20	38.34961153922649	40.0	37.0	41.0	34.0	41.0
21	38.26689354501631	40.0	37.0	41.0	34.0	41.0
22	38.21909440180829	40.0	37.0	41.0	34.0	41.0
23	38.167544820575635	40.0	37.0	41.0	34.0	41.0
24	38.03466313931867	40.0	37.0	41.0	34.0	41.0
25	37.88582631178515	40.0	36.0	41.0	33.0	41.0
26	37.75249003197975	40.0	36.0	41.0	33.0	41.0
27	37.68733090670312	40.0	36.0	41.0	33.0	41.0
28	37.62107948213649	40.0	36.0	41.0	33.0	41.0
29	37.57879550326269	40.0	36.0	41.0	33.0	41.0
30	37.425545281488056	40.0	36.0	41.0	33.0	41.0
31	37.337451028055675	40.0	36.0	41.0	33.0	41.0
32	37.214406354277486	40.0	35.0	41.0	32.0	41.0
33	37.076704034246426	40.0	35.0	41.0	32.0	41.0
34	36.93678870322607	39.0	35.0	41.0	31.0	41.0
35	36.79109302538491	39.0	35.0	41.0	31.0	41.0
36	36.61916953684616	39.0	35.0	41.0	30.0	41.0
37	36.52795623226198	39.0	35.0	41.0	30.0	41.0
38	36.4275941318904	39.0	35.0	41.0	30.0	41.0
39	36.35131628378314	39.0	35.0	41.0	30.0	41.0
40	36.21918910983922	39.0	35.0	41.0	30.0	41.0
41	36.09639699081016	39.0	35.0	40.0	29.0	41.0
42	35.99874354012306	39.0	35.0	40.0	29.0	41.0
43	35.91221828306968	39.0	35.0	40.0	28.0	41.0
44	35.75715913790437	39.0	35.0	40.0	28.0	41.0
45	35.701445560245354	39.0	35.0	40.0	27.0	41.0
46	35.52794991839325	38.0	35.0	40.0	27.0	41.0
47	35.491667271745754	38.0	35.0	40.0	27.0	41.0
48	35.37581409444916	38.0	34.0	40.0	26.0	41.0
49	35.24315339859769	38.0	34.0	40.0	26.0	41.0
50	35.10910049469162	38.0	34.0	40.0	26.0	41.0
51	33.287208417649786	36.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	11.0
10	19.0
11	11.0
12	9.0
13	15.0
14	10.0
15	13.0
16	28.0
17	64.0
18	117.0
19	198.0
20	368.0
21	562.0
22	893.0
23	1383.0
24	2130.0
25	2695.0
26	3466.0
27	3885.0
28	4307.0
29	5022.0
30	6097.0
31	7860.0
32	10364.0
33	15358.0
34	21381.0
35	27697.0
36	35667.0
37	61022.0
38	64196.0
39	41899.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.89461206012066	19.540476633950306	26.06933259250607	13.49557871342297
2	31.693411162288527	25.937688429519863	26.147624564737676	16.221275843453938
3	31.60785824101931	23.980704817166146	28.54531621433059	15.866120727483954
4	28.647285194293527	25.742589885813683	27.634224956828923	17.975899963063867
5	22.980588010594673	38.35012296259349	22.479898220436098	16.189390806375744
6	27.789546127546462	36.5055262136045	22.822425598949373	12.882502059899673
7	49.174619510485755	43.35481101012429	4.819060306917159	2.651509172472795
8	85.37392309076502	5.548312145042193	6.626720923845272	2.4510438403475154
9	78.29923318064294	9.130169874638137	8.47068628596143	4.099910658757494
10	40.35982737882897	33.957248794840304	14.085925439524186	11.59699838680654
11	33.5803108317575	25.6219949931021	24.767728554155628	16.029965620984772
12	31.421914806969248	23.27134166553543	26.952327134166552	18.354416393328766
13	24.864330745699466	26.39765376638054	28.67443482982545	20.063580658094537
14	20.355597086780968	28.75241110862064	29.433361850973753	21.458629953624634
15	19.980237590880247	27.218456701066728	32.90662103844199	19.894684669611035
16	23.530525976834415	25.31008987792135	31.63627065029691	19.523113494947328
17	22.490316103837884	25.0177577557985	29.27898776056547	23.212938379798146
18	22.811060635238334	26.191190258963324	29.12903337826703	21.86871572753131
19	24.93473038202063	26.94853881292954	27.318847213847576	20.797883591202254
20	26.718082604344573	25.736907403958163	28.385575335503198	19.159434656194062
21	25.817409230244692	26.368925663666527	27.668635541398455	20.14502956469032
22	24.774673809756823	25.90580339244167	27.475746851747207	21.843775946054304
23	24.24178328908364	26.40838734321875	27.990327153108158	21.359502214589458
24	22.522832527788914	25.195493160501698	30.57490931706039	21.706764994648996
25	22.10011901642553	27.899091749983423	28.290235917705036	21.71055331588601
26	22.61690917184141	29.22216294201027	26.39765376638054	21.763274119767775
27	22.00667375924587	28.834807095525676	28.177533360903894	20.980985784324556
28	21.446317909604343	28.3625297146447	29.94794215233471	20.243210223416245
29	21.599429226266956	26.524562527820482	30.037599088277357	21.8384091576352
30	22.256071574015905	27.33115925786787	28.9667669519483	21.446002216167923
31	24.385739496090135	27.503843567588387	27.06124136973068	21.049175566590794
32	24.755732203571757	28.006111824929047	27.28885633738789	19.949299634111306
33	23.984808831839576	28.454712198078692	26.125841717624848	21.434637252456884
34	22.210927412608164	28.724945779652295	27.842898318301064	21.221228489438477
35	24.753206656080415	25.976834415635665	27.734299776173355	21.53565915211057
36	23.99301686118644	28.500172052922846	26.53655887840436	20.970252207486354
37	23.07813728244776	29.621199445642326	27.868469486650906	19.43219378525901
38	22.680679245997794	27.820484084315407	27.122485896395727	22.376350773291072
39	23.694370870335234	27.165735897184963	27.37882896676695	21.76106426571285
40	23.648595322054657	27.107016918011258	28.98539286469695	20.258994895237134
41	20.792201109346735	27.238976774433883	29.5372249915552	22.431597124664183
42	21.97857704340469	27.532887363738823	27.81795853682406	22.67057705603243
43	23.34205699529301	26.62400596029208	29.15681440067179	20.877122643743114
44	21.662883606986927	28.695270596629026	27.8009110912575	21.840934705126546
45	21.912912808629795	29.173861846238353	26.412491357892176	22.50073398723967
46	21.486410976029397	30.111155658962694	26.724080779636513	21.678352585371396
47	22.593547857546493	27.966018758503992	28.327172049765913	21.1132613341836
48	22.752973042937462	27.12943115199692	28.711055268449915	21.406540536615704
49	21.316252213800226	27.558774225525077	28.195212193343288	22.929761367331412
50	21.453263165205534	28.22678153698506	28.016214014894413	22.303741282914988
51	21.904704779282934	27.506053421643312	26.991473120282354	23.5977686787914
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	281.0
1	717.5
2	1154.0
3	2251.0
4	3348.0
5	2360.0
6	1372.0
7	1205.0
8	1038.0
9	1057.5
10	1077.0
11	1097.0
12	1117.0
13	1038.5
14	960.0
15	1004.0
16	1048.0
17	1054.5
18	1061.0
19	1184.5
20	1308.0
21	1475.5
22	1643.0
23	1798.5
24	1954.0
25	2406.0
26	3496.5
27	4135.0
28	4791.5
29	5448.0
30	6607.0
31	7766.0
32	8845.0
33	9924.0
34	10671.5
35	11419.0
36	11830.0
37	12241.0
38	13021.0
39	13801.0
40	15594.0
41	17387.0
42	18642.5
43	19898.0
44	20464.0
45	21030.0
46	21387.0
47	21744.0
48	20980.5
49	20217.0
50	19318.0
51	18419.0
52	17862.0
53	17305.0
54	16963.0
55	16621.0
56	16451.0
57	16281.0
58	15639.5
59	14998.0
60	14484.5
61	13971.0
62	12395.5
63	10820.0
64	9032.5
65	7245.0
66	6282.0
67	5319.0
68	4515.0
69	3711.0
70	3104.5
71	2498.0
72	2139.5
73	1781.0
74	1441.5
75	848.0
76	594.0
77	494.5
78	395.0
79	311.0
80	227.0
81	178.5
82	130.0
83	97.5
84	65.0
85	47.0
86	29.0
87	19.0
88	9.0
89	6.0
90	3.0
91	4.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	316763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.708484863060864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.65599992072023	20.81852279655283
2	7.977999990366548	5.059405838640748
3	4.731999994286099	4.501336505724747
4	3.2720001154987646	4.15000664536904
5	2.549999998206927	4.042831817197479
6	2.0499999975389005	3.900143633474222
7	1.812999997810973	4.024123809112303
8	1.598999998069206	4.0561493787849425
9	1.5199999981645966	4.3377207240289195
>10	8.764999989416243	42.51473645305538
>50	0.03899999995290741	0.8929109326656021
>100	0.023999999971019946	1.2109470354580734
>500	0.0019999999975849956	0.4911644299357307
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	985	0.310958034871497	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	564	0.17805109813961859	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	465	0.14679744793426	No Hit
GATACTAAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	324	0.10228467339935535	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019257299621483568	0.0
2	0.0	0.0	0.0	0.08744708188772048	0.0
3	0.0	0.0	0.0	0.11143978305547049	0.0
4	0.0	0.0	0.0	0.15342701009903303	0.0
5	0.0	0.0	0.0	0.30780110050731935	0.0
6	0.0	0.0	0.0	0.38041059088340495	0.0
7	0.0	0.0	0.0	0.46438504497053	0.0
8	0.0	0.0	0.0	0.6717956326970006	0.0
9	0.0	0.0	0.0	0.7437737362002507	0.0
10	0.0	0.0	0.0	1.0231624274299713	0.0
11	0.0	0.0	0.0	1.2662463734716491	0.0
12	0.0	0.0	0.0	1.557315721848827	0.0
13	0.0	0.0	0.0	1.642868643118041	0.0
14	0.0	0.0	0.0	1.6807518554881726	0.0
15	0.0	0.0	0.0	1.742943462462472	0.0
16	0.0	0.0	0.0	1.8755347057579326	0.0
17	0.0	0.0	0.0	2.027067555238459	0.0
18	0.0	0.0	0.0	2.1880712078115185	0.0
19	0.0	0.0	0.0	2.2865675599738604	0.0
20	0.0	0.0	0.0	2.391693474300976	0.0
21	0.0	0.0	0.0	2.5400693894173245	0.0
22	0.0	0.0	0.0	2.691602238897851	0.0
23	0.0	0.0	0.0	2.8387153802685288	0.0
24	0.0	0.0	0.0	2.9738321710553315	0.0
25	0.0	0.0	0.0	3.0874818081657263	0.0
26	0.0	0.0	0.0	3.2008157518397033	0.0
27	0.0	0.0	0.0	3.3087829070945785	0.0
28	0.0	0.0	0.0	3.425589478569151	0.0
29	0.0	0.0	0.0	3.559127802173865	0.0
30	0.0	0.0	0.0	3.7008741551254407	0.0
31	0.0	0.0	0.0	3.8388321868400035	0.0
32	0.0	0.0	0.0	3.9761588316817305	0.0
33	0.0	0.0	0.0	4.112538396214204	0.0
34	0.0	0.0	0.0	4.256810296657123	0.0
35	0.0	0.0	0.0	4.404870518337053	0.0
36	0.0	0.0	0.0	4.578501908366823	0.0
37	0.0	0.0	0.0	4.740136947812718	0.0
38	0.0	0.0	0.0	4.898930746330853	0.0
39	0.0	0.0	0.0	5.070352282305699	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCACT	25	3.8843857E-5	45.000004	21
ATAACGG	25	3.8843857E-5	45.000004	2
TATGGGT	20	7.02554E-4	45.0	4
CCTCGTG	20	7.02554E-4	45.0	15
TAGTTCG	20	7.02554E-4	45.0	20
TAGTTAG	40	6.7884685E-9	45.0	2
CACCTCG	20	7.02554E-4	45.0	34
AATGCGT	20	7.02554E-4	45.0	41
TCTCGCC	20	7.02554E-4	45.0	16
CAACTCC	20	7.02554E-4	45.0	35
GCGCTAA	20	7.02554E-4	45.0	1
ATTCTCG	20	7.02554E-4	45.0	13
CGTTATT	135	0.0	41.666664	1
CGTTTTA	190	0.0	41.447372	1
GGATTCC	105	0.0	40.714287	8
AAGGCGA	140	0.0	40.17857	6
CTCGTCC	45	1.920671E-8	40.000004	37
TAACGGG	80	0.0	39.375	3
ATGGGAC	110	0.0	38.863632	5
CTACGAA	110	0.0	38.863632	11
>>END_MODULE