##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934569.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	239231
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.454982840852566	33.0	31.0	34.0	31.0	34.0
2	32.66874276327064	34.0	31.0	34.0	31.0	34.0
3	32.53827472192149	34.0	31.0	34.0	31.0	34.0
4	36.21179529408814	37.0	35.0	37.0	35.0	37.0
5	32.65455981875259	37.0	35.0	37.0	16.0	37.0
6	34.42522917180466	37.0	35.0	37.0	25.0	37.0
7	20.030593861163478	32.0	0.0	37.0	0.0	37.0
8	27.810037160735856	35.0	17.0	37.0	17.0	37.0
9	35.06221601715497	37.0	32.0	39.0	32.0	39.0
10	36.70205366361383	37.0	35.0	39.0	34.0	39.0
11	37.20241523882775	39.0	37.0	39.0	34.0	39.0
12	37.46999343730537	39.0	37.0	39.0	35.0	39.0
13	37.18469596331579	39.0	37.0	39.0	34.0	39.0
14	38.49556286601653	40.0	38.0	41.0	35.0	41.0
15	38.72256521939046	40.0	38.0	41.0	35.0	41.0
16	38.85200078585133	40.0	38.0	41.0	35.0	41.0
17	38.8550271494915	40.0	38.0	41.0	35.0	41.0
18	38.79054553966668	40.0	38.0	41.0	35.0	41.0
19	38.73069125656792	40.0	38.0	41.0	35.0	41.0
20	38.674966873022306	40.0	38.0	41.0	35.0	41.0
21	38.57574478223976	40.0	38.0	41.0	35.0	41.0
22	38.57096697334376	40.0	38.0	41.0	35.0	41.0
23	38.515150628472064	40.0	37.0	41.0	35.0	41.0
24	38.41187387922134	40.0	37.0	41.0	34.0	41.0
25	38.318792296984924	40.0	37.0	41.0	34.0	41.0
26	38.19196926819685	40.0	37.0	41.0	34.0	41.0
27	38.146323009977806	40.0	37.0	41.0	34.0	41.0
28	38.079834971220286	40.0	37.0	41.0	34.0	41.0
29	38.02100898294953	40.0	36.0	41.0	34.0	41.0
30	37.88006571054755	40.0	36.0	41.0	34.0	41.0
31	37.73396006370412	40.0	36.0	41.0	33.0	41.0
32	37.65633634437009	40.0	36.0	41.0	33.0	41.0
33	37.50491366085499	40.0	36.0	41.0	33.0	41.0
34	37.34705786457441	40.0	36.0	41.0	33.0	41.0
35	37.164911738027264	40.0	35.0	41.0	32.0	41.0
36	37.01176686967826	40.0	35.0	41.0	32.0	41.0
37	36.92256856343869	40.0	35.0	41.0	31.0	41.0
38	36.77817674130861	40.0	35.0	41.0	31.0	41.0
39	36.624856310427994	40.0	35.0	41.0	31.0	41.0
40	36.45084458117886	39.0	35.0	41.0	30.0	41.0
41	36.299940225138045	39.0	35.0	41.0	30.0	41.0
42	36.21500976044074	39.0	35.0	41.0	30.0	41.0
43	36.15100049742717	39.0	35.0	41.0	29.0	41.0
44	35.988379432431415	39.0	35.0	41.0	29.0	41.0
45	35.93081582236416	39.0	35.0	41.0	29.0	41.0
46	35.848263811964166	39.0	35.0	40.0	28.0	41.0
47	35.743720504449676	39.0	35.0	40.0	28.0	41.0
48	35.63092993800971	38.0	35.0	40.0	28.0	41.0
49	35.52788309207419	38.0	35.0	40.0	27.0	41.0
50	35.372723434671926	38.0	35.0	40.0	27.0	41.0
51	33.50763906015525	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	18.0
10	14.0
11	10.0
12	7.0
13	12.0
14	2.0
15	12.0
16	23.0
17	32.0
18	61.0
19	119.0
20	207.0
21	350.0
22	592.0
23	877.0
24	1332.0
25	1945.0
26	2377.0
27	2598.0
28	2870.0
29	3010.0
30	3509.0
31	4468.0
32	6277.0
33	10375.0
34	15190.0
35	19749.0
36	25723.0
37	48305.0
38	53756.0
39	35396.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.67266365980998	21.09634620931234	26.702225046085164	13.528765084792523
2	32.04517809146808	24.396921803612408	26.832224920683355	16.72567518423616
3	31.081674197741933	23.674607387838535	28.528075374846907	16.71564303957263
4	28.130551642554686	25.70695269425785	28.284377860728753	17.87811780245871
5	23.20017054645928	36.974305169480544	23.31804824625571	16.507476037804462
6	26.89785186702392	34.48716930498138	24.434542346100628	14.180436481894068
7	47.89513064778394	45.52461846499827	4.371507037131475	2.2087438500863183
8	87.59023705121828	3.9175524911069215	6.294334764307301	2.1978756933674983
9	82.26107820474772	6.392148174776681	7.742725650103874	3.6040479703717327
10	44.07748159728463	27.874731953634775	15.199953183324904	12.847833265755693
11	34.223825507563824	24.41865811705005	23.885700431800228	17.471815943585906
12	31.76260601677876	22.176473784751977	27.638140541986616	18.422779656482646
13	25.92222579849601	25.785955833483122	28.66685337602568	19.624964991995185
14	20.649079759730135	26.760745889955732	31.10299250515192	21.487181845162205
15	20.55335637939899	24.825795987978147	34.190803031379716	20.43004460124315
16	24.898947042816356	24.016118312426066	30.481417542040955	20.60351710271662
17	25.292708720859753	24.170362536627778	28.01601799097943	22.520910751533037
18	25.70151861589844	24.272774013401275	28.93479523974736	21.09091213095293
19	26.443897320999373	26.014605130605982	26.30261128365471	21.238886264739936
20	26.507016231174052	25.43483077025971	28.102127232674697	19.956025765891543
21	26.081486095029486	25.197403346556257	29.15132236206846	19.56978819634579
22	25.86412296065309	22.84361140487646	29.11495583766318	22.177309796807272
23	23.729366177460278	24.49013714777767	29.40547002687779	22.375026647884262
24	22.03644176549026	24.930297494889878	31.692799010161725	21.340461729458138
25	23.966375595136082	26.338977808059994	27.74598609711952	21.948660499684404
26	21.619271749898633	26.90621198757686	28.073284816767057	23.401231445757446
27	21.27274475297934	27.580455710171343	29.206917163745498	21.93988237310382
28	20.476443270311957	26.82888087246218	30.054215381785802	22.640460475440054
29	21.31036529546756	25.57402677746613	30.102704080992847	23.01290384607346
30	22.773804398259422	25.936019997408362	30.157462870614594	21.13271273371762
31	24.52859370232119	26.804218516831014	28.368815078313432	20.29837270253437
32	26.119106637517714	25.658045989023158	26.624475924942836	21.59837144851629
33	24.886406861986952	26.563865050934034	26.772868064757493	21.77686002232152
34	22.059014090983194	25.532644180729086	29.612801016590662	22.79554071169706
35	22.433547491754833	27.42579347994198	29.052254933516142	21.088404094787048
36	25.11589217116511	27.32547203330672	27.12900920031267	20.429626595215503
37	22.910492369299966	28.56026183897572	27.376050762652	21.153195029072318
38	22.99785562907817	27.521516860273127	28.063252672103534	21.41737483854517
39	22.513386643035393	27.049588055059754	27.103510832626203	23.333514469278647
40	25.31235500415916	25.497949680434388	27.83167733278714	21.35801798261931
41	21.267310674619928	26.0664378780342	28.75505264785918	23.911198799486687
42	22.04271185590496	25.128850358022163	28.828621708725038	23.999816077347834
43	22.52968887811362	25.84196864118781	28.786821105960346	22.841521374738225
44	21.97081481914969	26.853961234120998	28.37048710242402	22.804736844305296
45	21.216313939247	28.399747524359302	26.84225706534688	23.541681471046815
46	22.088274512918478	27.63605051184838	27.677015102557778	22.598659872675363
47	21.632229936755685	26.919588180461563	29.55846023299656	21.88972164978619
48	21.84374098674503	26.49238602020641	29.686370077456516	21.977502915592044
49	22.57901358937596	26.55341490024286	28.58994026693865	22.27763124344253
50	21.607985587152168	26.81257863738395	29.094891548336125	22.484544227127756
51	21.552808791502773	27.81412107962597	27.729683862041295	22.903386266829965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	180.0
1	472.0
2	764.0
3	1488.0
4	2212.0
5	1540.0
6	868.0
7	789.0
8	710.0
9	720.5
10	731.0
11	750.5
12	770.0
13	729.5
14	689.0
15	666.0
16	643.0
17	719.0
18	795.0
19	829.0
20	863.0
21	888.0
22	913.0
23	1013.0
24	1113.0
25	1387.0
26	2001.5
27	2342.0
28	2932.0
29	3522.0
30	4235.0
31	4948.0
32	5957.0
33	6966.0
34	7847.0
35	8728.0
36	9174.0
37	9620.0
38	10252.0
39	10884.0
40	11829.0
41	12774.0
42	13626.0
43	14478.0
44	14737.0
45	14996.0
46	15413.0
47	15830.0
48	15609.0
49	15388.0
50	15414.5
51	15441.0
52	14800.5
53	14160.0
54	13679.5
55	13199.0
56	13080.5
57	12962.0
58	12374.0
59	11786.0
60	10926.5
61	10067.0
62	9040.5
63	8014.0
64	7176.5
65	6339.0
66	5492.0
67	4645.0
68	3956.5
69	3268.0
70	2729.5
71	2191.0
72	1845.0
73	1499.0
74	1230.0
75	754.0
76	547.0
77	447.0
78	347.0
79	277.0
80	207.0
81	150.5
82	94.0
83	77.5
84	61.0
85	42.5
86	24.0
87	21.0
88	18.0
89	12.5
90	7.0
91	4.5
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	239231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.79986707408321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.81028209976888	24.12020181331015
2	9.7922126751374	7.40288674962693
3	5.656371296818499	6.414302494241967
4	4.044056663238563	6.114592172419126
5	3.2445343860929574	6.132148425580297
6	2.662862577270566	6.039351087442681
7	2.17076380364706	5.743820825896309
8	1.8511760607769632	5.5979367222475345
9	1.5138948788552344	5.150252266637685
>10	5.2372579592829736	26.39917067604115
>50	0.011058399407269793	0.2616717733069711
>100	0.0044233597629079165	0.35530512349988086
>500	0.0011058399407269791	0.2683598697493218
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	642	0.2683598697493218	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	423	0.17681654969464658	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008778126580585292	0.0
2	0.0	0.0	0.0	0.04765268715174873	0.0
3	0.0	0.0	0.0	0.06646295839586007	0.0
4	0.0	0.0	0.0	0.09070730799938136	0.0
5	0.0	0.0	0.0	0.17807056777758734	0.0
6	0.0	0.0	0.0	0.23826343575874365	0.0
7	0.0	0.0	0.0	0.30514440018225064	0.0
8	0.0	0.0	0.0	0.4773628835727811	0.0
9	0.0	0.0	0.0	0.549677926355698	0.0
10	0.0	0.0	0.0	0.7499028135985721	0.0
11	0.0	0.0	0.0	1.0145006290990717	0.0
12	0.0	0.0	0.0	1.2214136127842963	0.0
13	0.0	0.0	0.0	1.3188090172260285	0.0
14	0.0	0.0	0.0	1.3589375958801326	0.0
15	0.0	0.0	0.0	1.4162044216677605	0.0
16	0.0	0.0	0.0	1.5508023625700682	0.0
17	0.0	0.0	0.0	1.696268460191196	0.0
18	0.0	0.0	0.0	1.8835351605770154	0.0
19	0.0	0.0	0.0	1.9968147940693304	0.0
20	0.0	0.0	0.0	2.1301587168886975	0.0
21	0.0	0.0	0.0	2.288164995339233	0.0
22	0.0	0.0	0.0	2.4532773762597655	0.0
23	0.0	0.0	0.0	2.621733805401474	0.0
24	0.0	0.0	0.0	2.750897667944372	0.0
25	0.0	0.0	0.0	2.885077602819033	0.0
26	0.0	0.0	0.0	3.0205115557766344	0.0
27	0.0	0.0	0.0	3.194820069305399	0.0
28	0.0	0.0	0.0	3.3273279800694726	0.0
29	0.0	0.0	0.0	3.491186342907065	0.0
30	0.0	0.0	0.0	3.6525366695787755	0.0
31	0.0	0.0	0.0	3.793822706923434	0.0
32	0.0	0.0	0.0	3.9338547261851518	0.0
33	0.0	0.0	0.0	4.0676166550321655	0.0
34	0.0	0.0	0.0	4.228966981703876	0.0
35	0.0	0.0	0.0	4.433371929223219	0.0
36	0.0	0.0	0.0	4.6026643704202215	0.0
37	0.0	0.0	0.0	4.788259046695454	0.0
38	0.0	0.0	0.0	4.993082000242444	0.0
39	0.0	0.0	0.0	5.32581479824939	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTAC	20	7.022075E-4	45.000004	36
CGCATAG	20	7.022075E-4	45.000004	2
TCAATAG	20	7.022075E-4	45.000004	2
TACGAAT	40	6.7757355E-9	45.000004	12
CGAATAT	40	6.7757355E-9	45.000004	14
CGATGAT	20	7.022075E-4	45.000004	10
AATACGG	20	7.022075E-4	45.000004	2
TACACGG	20	7.022075E-4	45.000004	2
GCGTAAG	20	7.022075E-4	45.000004	2
TGCGTTG	20	7.022075E-4	45.000004	1
TACGTAG	20	7.022075E-4	45.000004	2
CGCGAAG	25	3.8815157E-5	45.0	2
CGACAAG	25	3.8815157E-5	45.0	2
CGTTTTA	145	0.0	41.89655	1
CGATTAT	45	1.9173967E-8	40.000004	10
TACGAAG	45	1.9173967E-8	40.000004	2
GCTACGA	45	1.9173967E-8	40.000004	10
CGAAGGA	85	0.0	39.705883	4
TTAATCG	40	3.4429286E-7	39.375004	20
ATCGTTG	40	3.4429286E-7	39.375004	23
>>END_MODULE