##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934567.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219357
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.690062318503628	31.0	31.0	34.0	30.0	34.0
2	31.877478266022965	31.0	31.0	34.0	30.0	34.0
3	31.338803867667774	31.0	31.0	34.0	28.0	34.0
4	35.35019625541925	37.0	35.0	37.0	33.0	37.0
5	31.73513952141942	37.0	35.0	37.0	0.0	37.0
6	33.75474226945117	37.0	35.0	37.0	17.0	37.0
7	21.13522249119016	32.0	0.0	37.0	0.0	37.0
8	28.344178667651363	35.0	17.0	37.0	17.0	37.0
9	35.001226311446636	37.0	32.0	39.0	32.0	39.0
10	36.22436484816988	37.0	35.0	39.0	32.0	39.0
11	36.59454223024567	38.0	35.0	39.0	32.0	39.0
12	36.9974242900842	39.0	37.0	39.0	34.0	39.0
13	36.791978373154265	39.0	37.0	39.0	33.0	39.0
14	38.18218702845134	40.0	37.0	41.0	33.0	41.0
15	38.36234540042032	40.0	38.0	41.0	34.0	41.0
16	38.4292363589947	40.0	38.0	41.0	34.0	41.0
17	38.420962175813855	40.0	38.0	41.0	34.0	41.0
18	38.27705065258916	40.0	37.0	41.0	34.0	41.0
19	38.14363799650798	40.0	37.0	41.0	34.0	41.0
20	38.02842398464603	40.0	36.0	41.0	34.0	41.0
21	37.89890908427814	40.0	36.0	41.0	33.0	41.0
22	37.85686802791796	39.0	36.0	41.0	33.0	41.0
23	37.766900532009466	39.0	35.0	41.0	33.0	41.0
24	37.61595025460779	39.0	35.0	41.0	33.0	41.0
25	37.4683689146004	39.0	35.0	41.0	33.0	41.0
26	37.34759775161039	39.0	35.0	41.0	33.0	41.0
27	37.318859211240124	39.0	35.0	41.0	33.0	41.0
28	37.24850813969921	39.0	35.0	41.0	33.0	41.0
29	37.26668399002539	39.0	35.0	41.0	33.0	41.0
30	37.1034933920504	39.0	35.0	41.0	33.0	41.0
31	36.89249488277101	39.0	35.0	41.0	32.0	41.0
32	36.71716881613078	39.0	35.0	41.0	31.0	41.0
33	36.43535879867066	39.0	35.0	41.0	30.0	41.0
34	36.10093135847044	39.0	35.0	40.0	30.0	41.0
35	35.77146842817872	39.0	35.0	40.0	27.0	41.0
36	35.47152358939993	39.0	35.0	40.0	25.0	41.0
37	35.303555391439524	39.0	35.0	40.0	24.0	41.0
38	35.19111767575231	39.0	35.0	40.0	23.0	41.0
39	35.14426710795644	39.0	35.0	40.0	23.0	41.0
40	35.05141390518652	39.0	35.0	40.0	23.0	41.0
41	34.96690326727663	38.0	35.0	40.0	22.0	41.0
42	34.87056715764712	38.0	34.0	40.0	22.0	41.0
43	34.77328738084493	38.0	34.0	40.0	21.0	41.0
44	34.60266141495371	38.0	34.0	40.0	20.0	41.0
45	34.535009140351114	38.0	34.0	40.0	20.0	41.0
46	34.38920116522382	38.0	34.0	40.0	20.0	41.0
47	34.374681455344486	38.0	34.0	40.0	20.0	41.0
48	34.257178936619304	38.0	34.0	40.0	19.0	41.0
49	34.113586527897446	38.0	33.0	40.0	18.0	41.0
50	33.92044019566278	38.0	33.0	40.0	18.0	41.0
51	32.26154168775102	36.0	31.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	6.0
10	8.0
11	6.0
12	1.0
13	4.0
14	7.0
15	4.0
16	10.0
17	24.0
18	56.0
19	142.0
20	288.0
21	538.0
22	912.0
23	1588.0
24	2599.0
25	3753.0
26	4395.0
27	4301.0
28	4139.0
29	4134.0
30	4600.0
31	5744.0
32	7441.0
33	10804.0
34	15275.0
35	20096.0
36	23809.0
37	38407.0
38	40248.0
39	26012.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.27947592281076	20.12016940421322	25.61760053246534	18.982754140510675
2	35.7636182114088	23.27165305871251	26.716266177965597	14.248462551913091
3	28.360617623326355	21.877578559152433	35.196050274210535	14.565753543310676
4	24.31242221583993	24.622875039319467	34.938479282630595	16.126223462210003
5	20.223197800845195	35.41259225828216	29.697707390236005	14.666502550636634
6	22.905127258304955	33.046130280775174	31.631541277460943	12.417201183458927
7	46.02497298923672	41.685471628441306	10.110003327908387	2.1795520544135814
8	80.39588433466905	3.794271438796118	13.470279042838843	2.339565183695984
9	76.29252770597702	6.067734332617605	14.705252168838925	2.9344857925664556
10	36.708197139822296	30.219231663452728	21.973312910005152	11.099258286719822
11	25.604835952351646	24.70310954289127	33.67706524068071	16.01498926407637
12	24.33977488751214	23.711575194773815	35.67608966205774	16.272560255656305
13	20.14752207588543	28.291780066284637	35.997027676344956	15.563670181484976
14	17.494312923681488	29.348504948554183	35.08390432035449	18.073277807409838
15	16.464484835222947	27.504934877847525	40.13275163318244	15.897828653747087
16	19.153252460600754	25.28389793806443	37.96322889171533	17.59962070961948
17	19.17923749868935	24.906431068987995	35.19650615207174	20.717825280250914
18	20.329417342505597	25.512292746527347	36.973973932903895	17.184315978063157
19	20.18672757194892	28.666511668193856	33.64515379039648	17.50160696946074
20	21.449965125343617	26.879470452276426	34.67407012313261	16.996494299247345
21	19.238501620645795	28.168237165898514	35.910410882716306	16.682850330739388
22	19.49698436794814	24.704021298613675	34.624379436261435	21.17461489717675
23	16.63908605606386	27.25237854274083	35.636884165994246	20.471651235201065
24	17.525768496104526	26.490606636669906	38.55632598914099	17.42729887808458
25	16.609453995085637	29.156124491126338	35.97833668403561	18.25608482975241
26	16.782231704481735	29.694516245207588	34.630305848457084	18.8929462018536
27	17.31378529064493	29.890087847663853	35.7636182114088	17.032508650282416
28	15.674904379618612	28.83837762186755	37.22653026801059	18.260187730503244
29	16.328633232584323	25.7452463336023	38.038904616675104	19.887215817138273
30	18.531435057919282	27.55827258760833	36.4820817206654	17.42821063380699
31	18.982298262649472	28.796892736498037	33.509302187757854	18.711506813094637
32	19.40535291784625	28.325059150152494	34.38185241410122	17.887735517900044
33	19.9442005497887	28.89490647665677	33.11314432637208	18.047748647182445
34	17.12459597824551	27.887872281258407	35.161403556759076	19.826128183737012
35	18.047748647182445	28.42717579106206	34.11789913246443	19.407176429291066
36	20.457974899364963	28.003665258004073	33.36524478361758	18.17311505901339
37	17.911897044543824	30.245216701541324	34.35997027676345	17.4829159771514
38	18.033616433485143	30.641830440788304	32.17768295518265	19.14687017054391
39	18.456215210820716	29.304740673878655	33.216628600865256	19.022415514435373
40	19.18835505591342	28.91542098041093	32.21506493980133	19.681159023874322
41	17.547650633442288	27.939842357435595	33.36205363858915	21.15045337053297
42	18.218702845133734	28.711187698591793	31.907347383489014	21.16276207278546
43	18.631728187384038	28.99565548398273	33.29276020368623	19.079856124947003
44	18.402877501059915	29.796632886117152	32.165830130791356	19.634659482031573
45	18.303040249456366	30.85199013480308	30.245216701541324	20.599752914199225
46	18.925769407860248	30.645933341539134	30.60946311264286	19.818834137957758
47	17.30330009983725	30.160879297218692	33.09490921192394	19.44091139102012
48	18.592066813459336	28.66058525599821	32.2647556266725	20.482592303869946
49	18.77578559152432	28.33736785240498	32.28116722967583	20.60567932639487
50	18.066439639491787	29.34896082641539	32.59344356460017	19.991155969492652
51	17.83120666311082	28.62502678282435	30.78907898995701	22.754687564107826
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	229.0
1	375.0
2	521.0
3	3849.0
4	7177.0
5	4978.0
6	2779.0
7	2461.5
8	2144.0
9	2118.0
10	2092.0
11	2121.5
12	2151.0
13	2134.0
14	2117.0
15	2002.5
16	1888.0
17	1824.0
18	1760.0
19	1809.0
20	1858.0
21	2017.5
22	2177.0
23	2324.0
24	2471.0
25	2750.5
26	3430.5
27	3831.0
28	4324.5
29	4818.0
30	5448.0
31	6078.0
32	7056.0
33	8034.0
34	8633.0
35	9232.0
36	9780.5
37	10329.0
38	11130.0
39	11931.0
40	12751.0
41	13571.0
42	14613.0
43	15655.0
44	16179.0
45	16703.0
46	17465.0
47	18227.0
48	17951.0
49	17675.0
50	16158.5
51	14642.0
52	12702.5
53	10763.0
54	9141.5
55	7520.0
56	6664.5
57	5809.0
58	5004.0
59	4199.0
60	3576.0
61	2953.0
62	2415.0
63	1877.0
64	1545.0
65	1213.0
66	1012.0
67	811.0
68	645.5
69	480.0
70	405.5
71	331.0
72	245.5
73	160.0
74	114.0
75	43.5
76	19.0
77	17.0
78	15.0
79	11.5
80	8.0
81	7.5
82	7.0
83	3.5
84	0.0
85	1.5
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	219357.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.96362897049486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.42667439306186	36.65700654037639
2	8.648637776310194	8.296401068062957
3	3.710348608533621	5.338853520326954
4	2.2725692698709983	4.360026770793639
5	1.6209932831013896	3.8874360197169686
6	1.2084800540228255	3.4777853357636634
7	1.012723910083349	3.400173971095058
8	0.8639111868140148	3.314905250224583
9	0.7091062693612099	3.0610179003863576
>10	3.4995892358952743	24.6922109594765
>50	0.0159798595231367	0.5025051273585044
>100	0.00798992976156835	0.6701665139890827
>500	9.987412201960437E-4	0.28023403193968077
>1k	0.0019974824403920875	2.0612769904896515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2821	1.2860314464548657	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1482	0.6756109903034778	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	585	0.26668854880400444	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	309	0.14086625911185874	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0656464120132934	0.0
2	0.0	0.0	0.0	0.2489093122170708	0.0
3	0.0	0.0	0.0	0.34282015162497664	0.0
4	0.0	0.0	0.0	0.4408338917837133	0.0
5	0.0	0.0	0.0	0.7006842726696664	0.0
6	0.0	0.0	0.0	0.9395642719402618	0.0
7	0.0	0.0	0.0	1.1921206070469599	0.0
8	0.0	0.0	0.0	1.8467612157350803	0.0
9	0.0	0.0	0.0	2.1221114439019497	0.0
10	0.0	0.0	0.0	2.7101938848543696	0.0
11	0.0	0.0	0.0	3.5011419740423144	0.0
12	0.0	0.0	0.0	4.11794472025055	0.0
13	0.0	0.0	0.0	4.411530062865557	0.0
14	0.0	0.0	0.0	4.535072963251686	0.0
15	0.0	0.0	0.0	4.72198288634509	0.0
16	0.0	0.0	0.0	5.14366990795826	0.0
17	0.0	0.0	0.0	5.698017387181626	0.0
18	0.0	0.0	0.0	6.282452805244419	0.0
19	0.0	0.0	0.0	6.6635666972104834	0.0
20	0.0	0.0	0.0	7.025533719006004	0.0
21	0.0	0.0	0.0	7.484602725237854	0.0
22	0.0	0.0	0.0	8.005215242732167	0.0
23	0.0	0.0	0.0	8.568680279179603	0.0
24	0.0	0.0	0.0	9.005411270212484	0.0
25	0.0	0.0	0.0	9.402480887320669	0.0
26	0.0	0.0	0.0	9.74575691680685	0.0
27	0.0	0.0	0.0	10.097238747794691	0.0
28	0.0	0.0	0.0	10.473793861148721	0.0
29	0.0	0.0	0.0	10.896392638484297	0.0
30	0.0	0.0	0.0	11.341785308880045	0.0
31	0.0	0.0	0.0	11.80814836089115	0.0
32	0.0	0.0	0.0	12.234850038977557	0.0
33	0.0	0.0	0.0	12.620522709555656	0.0
34	0.0	0.0	0.0	13.015768815219026	0.0
35	0.0	0.0	0.0	13.45249980625191	0.0
36	0.0	0.0	0.0	13.842275377580838	0.0
37	0.0	0.0	0.0	14.297697360923062	0.0
38	0.0	0.0	0.0	14.718472626813824	0.0
39	0.0	0.0	0.0	15.193953236048998	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGTC	20	7.0207956E-4	45.0	5
CGGGTAT	25	3.8804552E-5	45.0	6
ACACGTT	20	7.0207956E-4	45.0	17
AGATCGA	20	7.0207956E-4	45.0	15
CCGGGAC	20	7.0207956E-4	45.0	5
GATCGAC	20	7.0207956E-4	45.0	16
GGCGTTA	20	7.0207956E-4	45.0	32
GGGTACG	20	7.0207956E-4	45.0	7
GCGTTAA	20	7.0207956E-4	45.0	33
GGCCCGT	20	7.0207956E-4	45.0	43
CGTTAAT	20	7.0207956E-4	45.0	34
CAGGCGA	20	7.0207956E-4	45.0	6
ACTACCT	20	7.0207956E-4	45.0	29
GATTGCC	20	7.0207956E-4	45.0	9
TACTAAG	20	7.0207956E-4	45.0	2
TAGGCGA	40	6.7720975E-9	45.0	6
CACACGT	20	7.0207956E-4	45.0	16
CACGTTC	20	7.0207956E-4	45.0	18
TAGGATC	50	2.1827873E-11	45.0	6
GGCGATC	40	6.7720975E-9	45.0	8
>>END_MODULE