##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934565.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406271
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.958599063186888	33.0	31.0	34.0	30.0	34.0
2	32.14218588085293	33.0	31.0	34.0	30.0	34.0
3	32.02423997774884	33.0	31.0	34.0	30.0	34.0
4	35.78679994387982	37.0	35.0	37.0	35.0	37.0
5	32.04535642465251	37.0	35.0	37.0	0.0	37.0
6	33.93811027614573	37.0	35.0	37.0	19.0	37.0
7	20.792766404690465	32.0	0.0	37.0	0.0	37.0
8	28.1290714818434	35.0	17.0	37.0	17.0	37.0
9	34.90673712866535	37.0	32.0	39.0	32.0	39.0
10	36.23544382936513	37.0	35.0	39.0	32.0	39.0
11	36.66927740350653	38.0	35.0	39.0	33.0	39.0
12	37.00051443494613	39.0	37.0	39.0	33.0	39.0
13	36.752647863125844	39.0	37.0	39.0	33.0	39.0
14	38.048238737197586	40.0	37.0	41.0	33.0	41.0
15	38.24729306300474	40.0	38.0	41.0	33.0	41.0
16	38.34096452860283	40.0	38.0	41.0	34.0	41.0
17	38.336127855544696	40.0	37.0	41.0	34.0	41.0
18	38.244856265891485	40.0	37.0	41.0	34.0	41.0
19	38.19355553312936	40.0	37.0	41.0	34.0	41.0
20	38.15014116193378	40.0	37.0	41.0	34.0	41.0
21	38.07606991392445	40.0	37.0	41.0	34.0	41.0
22	38.0735469674183	40.0	37.0	41.0	34.0	41.0
23	38.00321952588297	40.0	37.0	41.0	34.0	41.0
24	37.90353237124973	40.0	36.0	41.0	33.0	41.0
25	37.74532762614117	40.0	36.0	41.0	33.0	41.0
26	37.60401800768452	40.0	36.0	41.0	33.0	41.0
27	37.56966162979883	40.0	36.0	41.0	33.0	41.0
28	37.47981027442274	40.0	36.0	41.0	33.0	41.0
29	37.47594585879868	40.0	36.0	41.0	33.0	41.0
30	37.32838671724046	40.0	36.0	41.0	32.0	41.0
31	37.1895262029532	39.0	35.0	41.0	32.0	41.0
32	37.09058731733252	39.0	35.0	41.0	31.0	41.0
33	36.939414824095245	39.0	35.0	41.0	31.0	41.0
34	36.773833229543825	39.0	35.0	41.0	31.0	41.0
35	36.65522028399763	39.0	35.0	41.0	30.0	41.0
36	36.4780799023312	39.0	35.0	41.0	30.0	41.0
37	36.39118470183695	39.0	35.0	41.0	30.0	41.0
38	36.255565866133686	39.0	35.0	41.0	30.0	41.0
39	36.142838154827686	39.0	35.0	41.0	29.0	41.0
40	36.042380578480866	39.0	35.0	40.0	29.0	41.0
41	35.88914296122539	39.0	35.0	40.0	28.0	41.0
42	35.79295593335483	39.0	35.0	40.0	27.0	41.0
43	35.70271813641633	39.0	35.0	40.0	27.0	41.0
44	35.57961803820603	38.0	35.0	40.0	27.0	41.0
45	35.479401680159306	38.0	34.0	40.0	26.0	41.0
46	35.35379832673265	38.0	34.0	40.0	26.0	41.0
47	35.30762963637572	38.0	34.0	40.0	26.0	41.0
48	35.18151923223661	38.0	34.0	40.0	26.0	41.0
49	35.07053420007827	38.0	34.0	40.0	25.0	41.0
50	34.91122181991823	38.0	34.0	40.0	24.0	41.0
51	33.08405473194001	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	15.0
9	24.0
10	22.0
11	20.0
12	12.0
13	10.0
14	12.0
15	20.0
16	47.0
17	97.0
18	153.0
19	274.0
20	489.0
21	852.0
22	1289.0
23	1967.0
24	2850.0
25	3913.0
26	4772.0
27	5387.0
28	6135.0
29	6943.0
30	8521.0
31	10705.0
32	14367.0
33	20292.0
34	27736.0
35	35638.0
36	45694.0
37	76527.0
38	79446.0
39	52033.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.24333511375412	19.450563786241204	26.736094872634276	13.570006227370401
2	30.241636740008516	24.36427901573088	28.75322137194139	16.640862872319214
3	30.82302207147446	23.5813041048955	30.12373514230649	15.47193868132355
4	27.443996741091535	26.105481316658093	29.104464753821958	17.346057188428414
5	21.738937802599743	37.8776727849145	24.686231604027853	15.6971578084579
6	26.31519355307172	34.867859138358384	25.236849295174896	13.580098013395
7	49.86449931203556	42.92282737384652	4.6936650659289	2.5190082481890164
8	87.19007755906772	4.1890757646989325	6.376285779688927	2.244560896544425
9	81.99034634517353	6.593874532024191	7.773382791289558	3.6423963315127095
10	40.640853026674314	32.125354750892875	14.72760792672871	12.506184295704099
11	31.43492890213675	27.256683346830073	24.51984020518324	16.788547545849937
12	28.468436093149645	23.98866766271774	28.904105880065277	18.638790364067333
13	23.886026814613892	26.06831400715286	30.660076648345562	19.385582529887685
14	20.446943050328475	27.91535699077709	31.31185834086115	20.325841618033284
15	20.140005070507126	26.173416266482224	34.290412064853264	19.39616659815739
16	24.65251027023834	24.596143953174114	31.52058601278457	19.23075976380298
17	25.132485459213182	23.80184655070138	28.902382892207417	22.163285097878017
18	25.026398635393615	24.731521570577275	30.103797711379844	20.138282082649265
19	25.371488489210403	25.964934735681354	27.82354635206549	20.840030423042748
20	25.333336615214968	27.018172599077957	28.935119661506732	18.713371124200346
21	25.109840475938473	26.200245648840305	29.692249754474233	18.99766412074699
22	24.92892675086334	23.725050520465405	29.29596254716679	22.050060181504463
23	22.68756568891208	25.899215055960184	29.883009124451416	21.530210130676323
24	22.08476607978418	25.3906874967694	31.66384014610937	20.860706277337048
25	22.88250945797263	26.548781478372813	29.42174066079046	21.146968402864097
26	22.007231626180555	27.645340179338422	28.30401382328544	22.04341437119558
27	21.339943042944242	27.276620777756722	30.139242033027212	21.244194146271823
28	20.21261670165973	27.318957050835525	30.75951766185625	21.708908585648494
29	20.88975092979809	26.398881534739125	30.983998365622945	21.727369169839836
30	22.341491270605086	26.629515766569607	29.636375719655106	21.3926172431702
31	23.968484090668543	27.307388418075618	28.697101195999714	20.02702629525612
32	25.055935570099763	26.70409652670262	27.912895579551577	20.32707232364604
33	24.202318157092186	26.420541953523635	28.070918180229455	21.306221709154727
34	22.233189176682558	27.3750772267772	29.42986331783465	20.961870278705593
35	23.01001055945416	27.262836874893853	29.074927819115814	20.65222474653618
36	24.085647265002915	27.257667911320276	28.29761415409911	20.359070669577694
37	22.989334705159855	28.475820326826184	28.014305722042675	20.520539245971285
38	22.58024815947976	27.723613056309702	28.64467313689631	21.05146564731423
39	22.610523517553556	27.371138968816382	27.979353682640408	22.038983830989658
40	23.801108127333727	26.039269354691818	29.04859071900283	21.11103179897162
41	21.500673195970176	25.57873931439852	29.29423955930893	23.62634793032237
42	22.320323134065685	26.4852770687546	28.597414041366477	22.596985755813236
43	22.245003950565017	26.605393936559587	28.928966133442945	22.220635979432448
44	21.964403070856644	26.843904684311703	28.703747006308593	22.487945238523054
45	21.884407206027504	27.342340457477892	27.968277332125602	22.804975004369005
46	22.345183387443356	28.135407154337866	27.87203615320808	21.647373305010696
47	21.52553344934785	26.527859482955957	30.088290820659118	21.858316247037077
48	21.974740998003796	26.25415055467902	29.70283382274393	22.068274624573252
49	22.591816792239662	26.290333299694048	29.115787245459313	22.00206266260698
50	20.8200929921161	27.98058438825316	29.463338510501615	21.735984109129127
51	21.594945245907287	26.972636491405982	28.352749765550584	23.07966849713615
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	245.0
1	566.0
2	887.0
3	2402.5
4	3918.0
5	2740.0
6	1562.0
7	1437.0
8	1312.0
9	1309.0
10	1306.0
11	1328.0
12	1350.0
13	1323.0
14	1296.0
15	1356.5
16	1417.0
17	1364.5
18	1312.0
19	1390.0
20	1468.0
21	1501.5
22	1535.0
23	1891.5
24	2248.0
25	2904.5
26	4386.0
27	5211.0
28	6296.0
29	7381.0
30	8334.0
31	9287.0
32	10894.5
33	12502.0
34	14390.0
35	16278.0
36	16888.5
37	17499.0
38	18357.0
39	19215.0
40	21104.0
41	22993.0
42	24325.5
43	25658.0
44	26640.0
45	27622.0
46	28205.0
47	28788.0
48	28411.0
49	28034.0
50	27387.5
51	26741.0
52	25101.0
53	23461.0
54	22252.0
55	21043.0
56	20320.5
57	19598.0
58	18921.5
59	18245.0
60	16649.5
61	15054.0
62	13449.5
63	11845.0
64	10246.0
65	8647.0
66	7333.5
67	6020.0
68	5124.0
69	4228.0
70	3634.0
71	3040.0
72	2503.0
73	1966.0
74	1504.5
75	856.5
76	670.0
77	509.5
78	349.0
79	289.5
80	230.0
81	163.0
82	96.0
83	70.5
84	45.0
85	33.0
86	21.0
87	17.0
88	13.0
89	11.0
90	9.0
91	10.5
92	12.0
93	8.5
94	5.0
95	3.5
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	406271.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.66161645989706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.82054488935546	25.59734037754576
2	8.071951056781737	5.918615474535857
3	4.200632044820321	4.620058827490673
4	2.8282080570389585	4.147467162238119
5	2.2858278305596826	4.190107160866882
6	1.8619430862954458	4.095710597995239
7	1.5346448946380973	3.938379377055274
8	1.3603632630358855	3.9898492956444556
9	1.1571734010087822	3.818146266484077
>10	6.853612976200097	38.471963567315974
>50	0.019671797505422618	0.45261510665566423
>100	0.004070027070087439	0.2663465819935255
>500	6.783378450145731E-4	0.17458011256718475
>1k	6.783378450145731E-4	0.31882009161129754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1282	0.3155529191106429	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	702	0.17279106803094485	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02239884215215952	0.0
2	0.0	0.0	0.0	0.11420948086375843	0.0
3	0.0	0.0	0.0	0.15162293149154135	0.0
4	0.0	0.0	0.0	0.1964206157958604	0.0
5	0.0	0.0	0.0	0.3145683546204381	0.0
6	0.0	0.0	0.0	0.42459343640082603	0.0
7	0.0	0.0	0.0	0.5287111312399851	0.0
8	0.0	0.0	0.0	0.8324492764681702	0.0
9	0.0	0.0	0.0	0.9491201685574407	0.0
10	0.0	0.0	0.0	1.2243059435696861	0.0
11	0.0	0.0	0.0	1.5620115637099374	0.0
12	0.0	0.0	0.0	1.8386741854574902	0.0
13	0.0	0.0	0.0	1.970359686022384	0.0
14	0.0	0.0	0.0	2.0284489909444683	0.0
15	0.0	0.0	0.0	2.1246901698619887	0.0
16	0.0	0.0	0.0	2.308803729530289	0.0
17	0.0	0.0	0.0	2.537468832380357	0.0
18	0.0	0.0	0.0	2.8028089624905546	0.0
19	0.0	0.0	0.0	2.9640313977615924	0.0
20	0.0	0.0	0.0	3.153313921003468	0.0
21	0.0	0.0	0.0	3.3837020117113945	0.0
22	0.0	0.0	0.0	3.639442638042095	0.0
23	0.0	0.0	0.0	3.8855837605932986	0.0
24	0.0	0.0	0.0	4.097265125987334	0.0
25	0.0	0.0	0.0	4.275717439836956	0.0
26	0.0	0.0	0.0	4.461800128485666	0.0
27	0.0	0.0	0.0	4.662897425609999	0.0
28	0.0	0.0	0.0	4.846764844155748	0.0
29	0.0	0.0	0.0	5.067307289961627	0.0
30	0.0	0.0	0.0	5.308279448939254	0.0
31	2.461411225512035E-4	0.0	0.0	5.543344220975654	0.0
32	2.461411225512035E-4	0.0	0.0	5.747395211570602	0.0
33	2.461411225512035E-4	0.0	0.0	5.960799564822495	0.0
34	2.461411225512035E-4	0.0	0.0	6.189218526550012	0.0
35	2.461411225512035E-4	0.0	0.0	6.451604963189595	0.0
36	2.461411225512035E-4	0.0	0.0	6.675839525833742	0.0
37	2.461411225512035E-4	0.0	0.0	6.9109042978701405	0.0
38	2.461411225512035E-4	0.0	0.0	7.199873975745254	0.0
39	2.461411225512035E-4	0.0	0.0	7.601084005503716	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATGCA	25	3.886337E-5	45.000004	10
GTGCGAA	25	3.886337E-5	45.000004	1
AACAACG	20	7.027895E-4	45.0	37
TAGCCGT	20	7.027895E-4	45.0	44
AATAGGC	20	7.027895E-4	45.0	4
GCTCGAT	20	7.027895E-4	45.0	19
CGTCAAA	20	7.027895E-4	45.0	20
CGTTTTA	295	0.0	41.94915	1
TACGAAT	100	0.0	40.500004	12
CGAATAT	100	0.0	40.500004	14
CGTTATT	130	0.0	39.80769	1
TACGGGT	40	3.4508594E-7	39.375	4
TTTACGG	35	6.2383224E-6	38.571426	2
TACTGAC	125	0.0	37.800003	20
GTAAGGA	215	0.0	37.67442	4
ACACGCG	60	1.546141E-10	37.499996	36
CGCATAG	30	1.1386231E-4	37.499996	2
TGAACGG	60	1.546141E-10	37.499996	2
AATACGG	30	1.1386231E-4	37.499996	2
CGCCAGA	30	1.1386231E-4	37.499996	39
>>END_MODULE