##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934564.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	274792
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.900532038778422	31.0	31.0	34.0	30.0	34.0
2	32.106960901336286	33.0	31.0	34.0	30.0	34.0
3	31.860479926635417	33.0	31.0	34.0	30.0	34.0
4	35.68916489563015	37.0	35.0	37.0	35.0	37.0
5	32.03346531194504	37.0	35.0	37.0	0.0	37.0
6	33.92427363242016	37.0	35.0	37.0	19.0	37.0
7	20.83695304084544	32.0	0.0	37.0	0.0	37.0
8	28.155848787446505	35.0	17.0	37.0	17.0	37.0
9	34.883191650411945	37.0	32.0	39.0	32.0	39.0
10	36.16718099507992	37.0	35.0	39.0	32.0	39.0
11	36.63065154735218	38.0	35.0	39.0	33.0	39.0
12	36.96498442458296	39.0	37.0	39.0	33.0	39.0
13	36.73475210340912	39.0	35.0	39.0	33.0	39.0
14	38.08374697953361	40.0	37.0	41.0	33.0	41.0
15	38.28950260560715	40.0	38.0	41.0	34.0	41.0
16	38.37535663338088	40.0	38.0	41.0	34.0	41.0
17	38.35315074674663	40.0	37.0	41.0	34.0	41.0
18	38.238016390579055	40.0	37.0	41.0	34.0	41.0
19	38.16545241491747	40.0	37.0	41.0	34.0	41.0
20	38.113023668811316	40.0	37.0	41.0	34.0	41.0
21	38.037195405979794	40.0	37.0	41.0	34.0	41.0
22	37.98930099857347	40.0	36.0	41.0	34.0	41.0
23	37.929866953914235	40.0	36.0	41.0	34.0	41.0
24	37.77087760924626	40.0	36.0	41.0	33.0	41.0
25	37.65193673760517	39.0	35.0	41.0	33.0	41.0
26	37.52053189321378	39.0	35.0	41.0	33.0	41.0
27	37.48398424990538	39.0	35.0	41.0	33.0	41.0
28	37.397828175492734	39.0	35.0	41.0	33.0	41.0
29	37.39736236862791	39.0	35.0	41.0	33.0	41.0
30	37.247616378933884	39.0	35.0	41.0	32.0	41.0
31	37.10873679000844	39.0	35.0	41.0	32.0	41.0
32	36.973983958776095	39.0	35.0	41.0	31.0	41.0
33	36.79932821916213	39.0	35.0	41.0	31.0	41.0
34	36.592593670849226	39.0	35.0	41.0	30.0	41.0
35	36.43790940056479	39.0	35.0	41.0	30.0	41.0
36	36.236800925791144	39.0	35.0	41.0	30.0	41.0
37	36.11494876124487	39.0	35.0	41.0	29.0	41.0
38	36.00197967917552	39.0	35.0	40.0	29.0	41.0
39	35.92486680834959	39.0	35.0	40.0	28.0	41.0
40	35.848747416227546	39.0	35.0	40.0	27.0	41.0
41	35.75039666365833	39.0	35.0	40.0	27.0	41.0
42	35.627139800285306	39.0	35.0	40.0	27.0	41.0
43	35.516306879385134	39.0	35.0	40.0	26.0	41.0
44	35.38780604966666	38.0	35.0	40.0	26.0	41.0
45	35.252412733995165	38.0	34.0	40.0	25.0	41.0
46	35.13966199889371	38.0	34.0	40.0	24.0	41.0
47	35.1120847768494	38.0	34.0	40.0	24.0	41.0
48	35.00378831989286	38.0	34.0	40.0	24.0	41.0
49	34.85523596028997	38.0	34.0	40.0	24.0	41.0
50	34.69817898628781	38.0	34.0	40.0	23.0	41.0
51	32.812101516783606	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	20.0
10	14.0
11	8.0
12	5.0
13	8.0
14	7.0
15	11.0
16	29.0
17	46.0
18	91.0
19	183.0
20	334.0
21	550.0
22	969.0
23	1540.0
24	2286.0
25	3024.0
26	3741.0
27	3966.0
28	4167.0
29	4666.0
30	5766.0
31	7179.0
32	9578.0
33	13855.0
34	19248.0
35	24996.0
36	31395.0
37	51290.0
38	52281.0
39	33530.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.22048676817375	21.296471512998924	27.539739148155697	14.943302570671635
2	33.263340999737984	24.210675711083294	26.47711723776529	16.048866051413434
3	30.891729016856385	23.27505895368133	29.83893272002096	15.994279309441323
4	26.774069114093567	25.69798247401671	29.656613001834113	17.871335410055604
5	22.039215115432764	36.40644560249206	25.1612128446243	16.39312643745087
6	25.340257358292817	34.69569710908614	26.390142362223063	13.573903170397974
7	48.993784389647445	42.62423942472852	5.996171649829689	2.3858045357943465
8	85.79725756208332	3.6864246411831494	8.210937727444758	2.305380069288771
9	79.89169990392733	7.210908614515707	9.677137616815628	3.2202538647413315
10	38.81881568604617	31.170121400914148	17.778901860316164	12.232161052723514
11	31.31532213455996	25.444336079652974	26.785350374101135	16.454991411685928
12	27.907653789047714	23.137136452298463	30.590046289557193	18.365163469096625
13	22.538501848670993	26.67035430434656	32.24256892485953	18.548574922122913
14	19.855381524935222	28.83053363998952	32.03441148213922	19.27967335293604
15	19.30223587295118	26.2522198608402	35.51013129931002	18.9354129668986
16	23.39551369763312	24.327855250516755	33.42637340242802	18.850257649422108
17	23.49631721447495	24.179379312352616	30.4004483391074	21.923855134065036
18	24.42902267897173	24.458135608023525	31.46852892369501	19.64431278930973
19	23.90608169087892	27.113962560773242	28.856371364522985	20.12358438382486
20	24.895557367026697	26.069172319427057	29.958659640746454	19.07661067279979
21	23.894072607645057	26.597935893330227	30.133701126670353	19.374290372354363
22	23.884246994090073	23.42207924539288	31.237808960959562	21.455864799557485
23	21.42966316341087	26.273326734402747	31.09588343183208	21.201126670354302
24	21.22150572069056	25.505473230661735	33.10867856415034	20.164342484497368
25	21.268450318786574	27.38835191708638	30.409910041049233	20.933287723077818
26	20.684736091298145	29.337826428716994	29.15950973827477	20.817927741710093
27	20.79318175201607	28.044120643977994	30.433928207516956	20.728769396488982
28	19.78551049521092	27.666744301144135	32.259672770677454	20.28807243296748
29	20.385600745290983	26.065897114908733	32.10646598154241	21.44203615825788
30	21.181111531631196	27.255524178287576	30.680660281230892	20.882704008850332
31	23.29034324143352	27.748624414102302	28.694066785059245	20.26696555940493
32	23.178258464584122	27.29591836734694	29.205726513144487	20.320096654924452
33	22.603642027424378	27.68712335148039	28.509563597193516	21.199671023901715
34	20.150149931584615	28.566697720457658	30.088939998253224	21.194212349704504
35	21.454409153104894	27.583408541733384	30.15771929313808	20.80446301202364
36	22.817985967568198	28.453521208768812	28.029564179452095	20.69892864421089
37	21.420565373082187	29.011761623336923	29.14276980406999	20.4249031995109
38	21.590876008035167	28.584165477888728	28.520117033974792	21.304841480101313
39	21.19166496841247	27.738798800547322	29.246484613816996	21.823051617223207
40	22.329616582724388	27.504075810067253	28.362543305481964	21.803764301726396
41	20.065358525721273	27.167093656292764	29.40842528166759	23.35912253631838
42	21.38926897435151	26.98368220326647	29.0154007394684	22.61164808291362
43	21.773195726222013	26.922908963870857	29.558720777897463	21.745174532009663
44	21.473696468601705	27.587411569478004	28.50192145331742	22.436970508602872
45	20.59594165769018	29.123118576960028	27.683848146962063	22.597091618387726
46	21.73898803458616	28.885484293574777	28.07068619173775	21.304841480101313
47	20.795365221694954	27.95459838714373	29.822920609042473	21.42711578211884
48	21.579958659640745	27.385804535794346	29.41242830941221	21.6218084951527
49	21.742627150717635	27.010975574252527	29.05433928207517	22.192057992954673
50	20.478762118256718	28.057585373664445	29.42698477393811	22.03666773414073
51	19.80843692683921	28.315598707385952	28.07760051238755	23.79836385338729
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	294.0
1	483.5
2	673.0
3	2416.5
4	4160.0
5	2995.5
6	1831.0
7	1601.0
8	1371.0
9	1360.0
10	1349.0
11	1303.5
12	1258.0
13	1245.5
14	1233.0
15	1156.5
16	1080.0
17	1125.5
18	1171.0
19	1203.0
20	1235.0
21	1219.0
22	1203.0
23	1385.5
24	1568.0
25	1963.5
26	3026.0
27	3693.0
28	4339.5
29	4986.0
30	5646.0
31	6306.0
32	7368.0
33	8430.0
34	9155.0
35	9880.0
36	10344.0
37	10808.0
38	11780.0
39	12752.0
40	14329.0
41	15906.0
42	17161.5
43	18417.0
44	19406.5
45	20396.0
46	20496.5
47	20597.0
48	20540.0
49	20483.0
50	19927.5
51	19372.0
52	17727.0
53	16082.0
54	14796.0
55	13510.0
56	12782.0
57	12054.0
58	11177.0
59	10300.0
60	9354.0
61	8408.0
62	7534.5
63	6661.0
64	5819.5
65	4978.0
66	4210.0
67	3442.0
68	2856.0
69	2270.0
70	1964.0
71	1658.0
72	1393.0
73	1128.0
74	894.5
75	522.0
76	383.0
77	296.5
78	210.0
79	163.0
80	116.0
81	96.0
82	76.0
83	47.0
84	18.0
85	16.0
86	14.0
87	9.5
88	5.0
89	4.5
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	274792.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.751280096868705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.67939036636416	32.79423865824319
2	9.37467980801208	8.578072034296401
3	4.683150951699562	6.427804527813717
4	3.0513988987942007	5.584216228240407
5	2.2926478807051556	5.244578767681699
6	1.7503284616833412	4.804786062719752
7	1.4195526956292739	4.546244709299994
8	1.202732458707668	4.402123967994407
9	0.9228230250426597	3.7998301228709757
>10	3.6049031001292997	22.290281360183105
>50	0.011995012977772154	0.33329675856272933
>100	0.0047980051911088615	0.412687973280745
>500	7.99667531851477E-4	0.2711008760647447
>1k	7.99667531851477E-4	0.510737952748156
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1396	0.5080206119537687	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	741	0.2696585053422225	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	279	0.10153134006812427	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03821071938047687	0.0
2	0.0	0.0	0.0	0.1484759381641387	0.0
3	0.0	0.0	0.0	0.20924917755975428	0.0
4	0.0	0.0	0.0	0.2703863285685173	0.0
5	0.0	0.0	0.0	0.4421526099740895	0.0
6	0.0	0.0	0.0	0.5946315758828495	0.0
7	0.0	0.0	0.0	0.7525692159888207	0.0
8	0.0	0.0	0.0	1.1157530059099245	0.0
9	0.0	0.0	0.0	1.264956767300358	0.0
10	0.0	0.0	0.0	1.6139480043087135	0.0
11	0.0	0.0	0.0	2.1361611691752307	0.0
12	0.0	0.0	0.0	2.499708870709482	0.0
13	0.0	0.0	0.0	2.666744301144138	0.0
14	0.0	0.0	0.0	2.74061835861306	0.0
15	0.0	0.0	0.0	2.8567061632070803	0.0
16	0.0	0.0	0.0	3.157661067279979	0.0
17	0.0	0.0	0.0	3.4913680165361436	0.0
18	0.0	0.0	0.0	3.8534600716178056	0.0
19	0.0	0.0	0.0	4.084180034353256	0.0
20	0.0	0.0	0.0	4.3214504061253605	0.0
21	0.0	0.0	0.0	4.629683542461207	0.0
22	0.0	0.0	0.0	4.937552767183907	0.0
23	0.0	0.0	0.0	5.304011761623337	0.0
24	0.0	0.0	0.0	5.550743835337273	0.0
25	0.0	0.0	0.0	5.808757169058779	0.0
26	0.0	0.0	0.0	6.051486215028094	0.0
27	0.0	0.0	0.0	6.297490465515735	0.0
28	0.0	0.0	0.0	6.550045125040031	0.0
29	0.0	0.0	0.0	6.829165332324085	0.0
30	0.0	0.0	0.0	7.133031529302163	0.0
31	0.0	0.0	0.0	7.423432996593787	0.0
32	0.0	0.0	0.0	7.7014614690384	0.0
33	0.0	0.0	0.0	7.9864042621328135	0.0
34	0.0	0.0	0.0	8.25824623715392	0.0
35	0.0	0.0	0.0	8.557381583161082	0.0
36	3.6391161314739876E-4	0.0	0.0	8.863795161431192	0.0
37	3.6391161314739876E-4	0.0	0.0	9.17312003260648	0.0
38	3.6391161314739876E-4	0.0	0.0	9.499548749599697	0.0
39	3.6391161314739876E-4	0.0	0.0	9.867463390491718	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTT	20	7.023907E-4	45.0	27
AACGTAT	20	7.023907E-4	45.0	30
AGCCGTT	25	3.8830334E-5	45.0	45
CGAAACA	25	3.8830334E-5	45.0	18
CGGTTTA	40	6.7830115E-9	45.0	1
CGCGCGA	20	7.023907E-4	45.0	14
AAGGTAC	25	3.8830334E-5	45.0	6
CGATTAG	20	7.023907E-4	45.0	2
TGACGCT	20	7.023907E-4	45.0	41
AAACGTA	20	7.023907E-4	45.0	29
GAGCGAT	20	7.023907E-4	45.0	6
ACGTATT	20	7.023907E-4	45.0	31
AATCGTT	25	3.8830334E-5	45.0	22
CGTTATT	125	0.0	44.999996	1
CGTTTTA	245	0.0	44.081635	1
CGTTTTT	610	0.0	40.204918	1
AGGACCG	45	1.9192157E-8	40.000004	7
CACAACG	45	1.9192157E-8	40.000004	12
GCGTAAG	40	3.4454206E-7	39.375	2
CGGTTTT	105	0.0	38.57143	1
>>END_MODULE