##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934563.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410957
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.790963531464364	31.0	31.0	34.0	30.0	34.0
2	31.981908082840786	33.0	31.0	34.0	30.0	34.0
3	31.81216039634317	33.0	31.0	34.0	30.0	34.0
4	35.628987947644156	37.0	35.0	37.0	33.0	37.0
5	31.966633978737434	37.0	35.0	37.0	0.0	37.0
6	33.85654460198999	37.0	35.0	37.0	19.0	37.0
7	20.90300201724268	32.0	0.0	37.0	0.0	37.0
8	28.153911479789855	35.0	17.0	37.0	17.0	37.0
9	34.75804767895425	37.0	32.0	39.0	30.0	39.0
10	36.091734658370584	37.0	35.0	39.0	32.0	39.0
11	36.56379134556657	38.0	35.0	39.0	32.0	39.0
12	36.85633776769832	39.0	35.0	39.0	33.0	39.0
13	36.55036415002056	39.0	35.0	39.0	32.0	39.0
14	37.865195142070824	40.0	37.0	41.0	33.0	41.0
15	38.05975564353448	40.0	37.0	41.0	33.0	41.0
16	38.152760507790354	40.0	37.0	41.0	33.0	41.0
17	38.108780237348434	40.0	37.0	41.0	33.0	41.0
18	38.02286613927978	40.0	37.0	41.0	33.0	41.0
19	37.97247400579623	40.0	37.0	41.0	34.0	41.0
20	37.93109011405086	40.0	36.0	41.0	34.0	41.0
21	37.82823263747789	40.0	36.0	41.0	33.0	41.0
22	37.80918928257701	39.0	36.0	41.0	33.0	41.0
23	37.737147682117595	39.0	36.0	41.0	33.0	41.0
24	37.62409692498242	39.0	35.0	41.0	33.0	41.0
25	37.4656910577019	39.0	35.0	41.0	33.0	41.0
26	37.34243485328149	39.0	35.0	41.0	33.0	41.0
27	37.294587998257725	39.0	35.0	41.0	33.0	41.0
28	37.192713592906316	39.0	35.0	41.0	32.0	41.0
29	37.231004703655124	39.0	35.0	41.0	32.0	41.0
30	37.03423229194295	39.0	35.0	41.0	32.0	41.0
31	36.90431115664169	39.0	35.0	41.0	31.0	41.0
32	36.79155970089328	39.0	35.0	41.0	31.0	41.0
33	36.62586839985692	39.0	35.0	40.0	31.0	41.0
34	36.42850711874965	39.0	35.0	40.0	30.0	41.0
35	36.26141907790839	39.0	35.0	40.0	30.0	41.0
36	36.0970539496833	39.0	35.0	40.0	29.0	41.0
37	35.98207841696333	39.0	35.0	40.0	29.0	41.0
38	35.89845652951525	39.0	35.0	40.0	29.0	41.0
39	35.822322043425466	39.0	35.0	40.0	28.0	41.0
40	35.677248471251254	38.0	35.0	40.0	27.0	41.0
41	35.584506408213024	38.0	35.0	40.0	27.0	41.0
42	35.477524412529775	38.0	35.0	40.0	26.0	41.0
43	35.371637421920056	38.0	34.0	40.0	26.0	41.0
44	35.23307304657178	38.0	34.0	40.0	25.0	41.0
45	35.15900447005404	38.0	34.0	40.0	25.0	41.0
46	35.00955817761955	38.0	34.0	40.0	24.0	41.0
47	34.98389369204077	38.0	34.0	40.0	24.0	41.0
48	34.87451971860803	38.0	34.0	40.0	24.0	41.0
49	34.71648615305251	38.0	34.0	40.0	24.0	41.0
50	34.49555062938458	38.0	34.0	40.0	23.0	41.0
51	32.464688519723474	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	20.0
10	10.0
11	8.0
12	3.0
13	2.0
14	11.0
15	18.0
16	28.0
17	73.0
18	162.0
19	282.0
20	477.0
21	844.0
22	1420.0
23	2178.0
24	3152.0
25	4261.0
26	5479.0
27	6044.0
28	6792.0
29	7881.0
30	9402.0
31	12064.0
32	16002.0
33	23461.0
34	31929.0
35	39676.0
36	46255.0
37	73559.0
38	73543.0
39	45903.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.534549843414275	20.284847319792583	25.262740384030447	14.917862452762698
2	31.647106631594056	24.25314570624177	26.38061889686756	17.719128765296613
3	30.88936312071579	24.041201390899776	28.931007380334194	16.138428108050235
4	28.112916923181746	25.578345179666	28.33045793112175	17.97827996603051
5	22.21400292488022	36.55175602313624	24.261419077908393	16.972821974075146
6	25.759629352949336	33.58088559143657	26.72980384809116	13.929681207522929
7	47.87678516243792	42.6806210868777	6.599717245356571	2.842876505327808
8	81.15301600897418	5.134600456982117	9.64456135313427	4.067822180909438
9	75.4665329949362	7.977720296770709	11.44182968047752	5.113917027815562
10	39.3323389065036	28.713709706854974	18.640149699360272	13.31380168728115
11	33.54973878045635	24.10373834732101	25.187306701187715	17.159216171034924
12	29.85324498670177	22.23760636757617	29.26145557807751	18.647693067644546
13	22.81893239438676	25.885189934713367	32.12939553286597	19.166482138033906
14	19.365043058032835	27.815562212104915	32.60633107600065	20.213063653861596
15	18.999311363475986	25.158106565893757	37.14305876283893	18.699523307791328
16	21.835131169441084	23.408045123942408	34.27365880128578	20.483164905330728
17	20.94696038758313	23.408045123942408	31.284538285027384	24.360456203447075
18	22.215462931644918	24.575077197857684	32.185119124385274	21.024340746112124
19	23.66670965575474	25.459841297264678	29.46561319067445	21.407835856306136
20	24.627637441386813	25.32138398907915	29.72038437111425	20.330594198419785
21	22.553697832133288	26.94272150127629	30.483481240129745	20.020099426460675
22	22.455147375516173	23.94897763026302	29.860301686064478	23.735573308156326
23	21.299552021257696	25.8102429207922	30.50586801052178	22.38433704742832
24	21.631703560226494	23.947760957959105	32.46690042997199	21.953635051842408
25	19.37501977092494	26.195441372211693	31.017600381548434	23.411938475314937
26	19.195682273327865	27.628681346223573	30.533364804590263	22.642271575858302
27	20.324024167978642	26.965351606129108	31.280401599194075	21.43022262669817
28	17.559501359022963	26.964378268285977	33.90014040398387	21.575979968707188
29	19.8079117766579	24.46144000467202	32.53211406546184	23.198534153208243
30	20.289227340086676	25.643558815155842	32.00334828218037	22.063865562577107
31	22.815282377475015	25.866209846772293	29.704567631163357	21.613940144589336
32	23.206077521492517	25.6399087982441	29.724521056947562	21.42949262331582
33	23.180040734188733	24.984609095355477	29.314989159449773	22.520361011006017
34	20.263190552782895	25.218940181089504	31.470445813065602	23.047423453062
35	21.012417357533757	24.894575344865764	30.86867969154924	23.224327606051244
36	23.395635066442473	25.286587161187178	29.616723890820694	21.70105388154965
37	21.265241862287297	26.692817010052146	31.914287869533798	20.127653258126763
38	21.10902113846461	27.273656367941175	29.268512277440216	22.348810216154003
39	22.231766340517378	25.463734648637203	30.519251405864843	21.78524760498057
40	21.97991517360697	24.075998218791746	30.242093455033007	23.701993152568274
41	20.15101336636193	25.49050143932334	29.521823451115324	24.83666174319941
42	21.734634037137706	24.70209778638641	29.592390444742396	23.970877731733488
43	22.122509167625807	24.057748134233023	30.062512622975152	23.757230075166014
44	19.626140934452994	25.11138634942342	30.984506894881946	24.277965821241636
45	20.55543524018328	26.288881805152364	28.93928075200082	24.216402202663538
46	21.386179089296448	25.904656691576005	29.757857878074834	22.951306341052714
47	20.436688023321175	24.602817326386944	32.078781965023104	22.881712685268777
48	20.728446041799995	24.123935107566	31.624963195662808	23.5226556549712
49	21.198081551111187	23.34015480938395	32.8632922665875	22.598471372917363
50	20.242263789155558	24.188418739673494	30.825609491990647	24.743707979180304
51	19.47478689984597	24.155081918546223	29.954958791309068	26.415172390298743
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	509.0
1	876.0
2	1243.0
3	3562.0
4	5881.0
5	4149.5
6	2418.0
7	2067.0
8	1716.0
9	1705.5
10	1695.0
11	1668.0
12	1641.0
13	1559.5
14	1478.0
15	1515.5
16	1553.0
17	1529.5
18	1506.0
19	1582.5
20	1659.0
21	1787.0
22	1915.0
23	1981.0
24	2047.0
25	2636.0
26	3836.0
27	4447.0
28	5362.0
29	6277.0
30	7288.5
31	8300.0
32	9155.0
33	10010.0
34	10725.5
35	11441.0
36	12365.0
37	13289.0
38	15470.5
39	17652.0
40	19872.0
41	22092.0
42	24374.5
43	26657.0
44	29409.5
45	32162.0
46	34019.5
47	35877.0
48	35940.5
49	36004.0
50	33919.5
51	31835.0
52	27959.0
53	24083.0
54	22123.0
55	20163.0
56	19408.0
57	18653.0
58	17456.5
59	16260.0
60	15196.0
61	14132.0
62	12711.5
63	11291.0
64	9432.0
65	7573.0
66	6374.0
67	5175.0
68	4259.0
69	3343.0
70	2760.5
71	2178.0
72	1805.0
73	1432.0
74	1167.0
75	760.5
76	619.0
77	449.0
78	279.0
79	204.0
80	129.0
81	114.5
82	100.0
83	77.0
84	54.0
85	39.0
86	24.0
87	21.0
88	18.0
89	12.0
90	6.0
91	6.5
92	7.0
93	6.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	410957.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.580042311727055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.33948452741774	27.936809739723373
2	8.184602755968836	6.151554357479735
3	3.3606496682829183	3.788800701868907
4	2.076010073456905	3.120661856003283
5	1.5264285106477207	2.8681624007983926
6	1.2635860482913923	2.8491370295579106
7	1.0363570089949632	2.726243817365993
8	0.8958698746687946	2.6933462236683936
9	0.7762447475310297	2.625417941082478
>10	6.472201720540741	41.73386604847587
>50	0.0411390385193393	1.004904425343351
>100	0.026120024456723366	1.758030599062214
>500	0.0	0.0
>1k	0.0013060012228361683	0.7430648595701262
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1933	0.47036551269354215	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1095	0.2664512345573868	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4333446078300162E-4	0.0	0.0	0.10122713568572868	0.0
2	2.4333446078300162E-4	0.0	0.0	0.4752322019092022	0.0
3	2.4333446078300162E-4	0.0	0.0	0.604442800584976	0.0
4	2.4333446078300162E-4	0.0	0.0	0.7672335548488042	0.0
5	2.4333446078300162E-4	0.0	0.0	1.2765325812676265	0.0
6	2.4333446078300162E-4	0.0	0.0	1.6038174310207638	0.0
7	2.4333446078300162E-4	0.0	0.0	1.9938825716559154	0.0
8	2.4333446078300162E-4	0.0	0.0	2.9248802186116794	0.0
9	2.4333446078300162E-4	0.0	0.0	3.2541117440510807	0.0
10	2.4333446078300162E-4	0.0	0.0	4.0768255559584095	0.0
11	2.4333446078300162E-4	0.0	0.0	5.123893740707666	0.0
12	2.4333446078300162E-4	0.0	0.0	5.97872770143835	0.0
13	2.4333446078300162E-4	0.0	0.0	6.310392571485581	0.0
14	2.4333446078300162E-4	0.0	0.0	6.444956528298581	0.0
15	2.4333446078300162E-4	0.0	0.0	6.699241039816818	0.0
16	2.4333446078300162E-4	0.0	0.0	7.204403380402329	0.0
17	2.4333446078300162E-4	0.0	0.0	7.8380463162812655	0.0
18	2.4333446078300162E-4	0.0	0.0	8.574863063532195	0.0
19	2.4333446078300162E-4	0.0	0.0	8.979284937353544	0.0
20	2.4333446078300162E-4	0.0	0.0	9.39246685176308	0.0
21	2.4333446078300162E-4	0.0	0.0	9.956272797397295	0.0
22	2.4333446078300162E-4	0.0	0.0	10.500611986168868	0.0
23	2.4333446078300162E-4	0.0	0.0	11.02353774239154	0.0
24	2.4333446078300162E-4	0.0	0.0	11.436719656801076	0.0
25	2.4333446078300162E-4	0.0	0.0	11.802208016897145	0.0
26	2.4333446078300162E-4	0.0	0.0	12.149689626895272	0.0
27	2.4333446078300162E-4	0.0	0.0	12.486464520618945	0.0
28	2.4333446078300162E-4	0.0	0.0	12.866309613901212	0.0
29	2.4333446078300162E-4	0.0	0.0	13.23666466321294	0.0
30	2.4333446078300162E-4	0.0	0.0	13.660066624975363	0.0
31	2.4333446078300162E-4	0.0	0.0	14.071058529237852	0.0
32	2.4333446078300162E-4	0.0	0.0	14.446523602226023	0.0
33	2.4333446078300162E-4	0.0	0.0	14.839508756390572	0.0
34	2.4333446078300162E-4	0.0	0.0	15.201103765114112	0.0
35	2.4333446078300162E-4	0.0	0.0	15.603335628788413	0.0
36	2.4333446078300162E-4	0.0	0.0	16.002160810011752	0.0
37	2.4333446078300162E-4	0.0	0.0	16.384682582362633	0.0
38	2.4333446078300162E-4	0.0	0.0	16.777667736527178	0.0
39	2.4333446078300162E-4	0.0	0.0	17.153619478436916	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAA	20	7.0279907E-4	45.000004	28
TGCGGGT	35	1.2093733E-7	45.000004	4
AATATCG	20	7.0279907E-4	45.000004	12
TCGGACG	20	7.0279907E-4	45.000004	1
ACGGCGT	30	2.1620854E-6	45.000004	33
TACGAAT	30	2.1620854E-6	45.000004	12
CGGGATC	35	1.2093733E-7	45.000004	6
CATACGG	20	7.0279907E-4	45.000004	2
TTAACGG	40	6.7975634E-9	45.000004	2
ACTCGAA	20	7.0279907E-4	45.000004	16
ATAACGG	45	3.8380676E-10	45.000004	2
ATACGAG	20	7.0279907E-4	45.000004	2
TATCGCA	20	7.0279907E-4	45.000004	1
GTATCTC	25	3.8864164E-5	45.0	22
GTACGAA	25	3.8864164E-5	45.0	1
CGTTTTA	385	0.0	43.246754	1
AGGCGAT	195	0.0	41.53846	7
ACTAATC	65	0.0	41.53846	10
CGTTATT	210	0.0	40.714287	1
AATAGGC	45	1.9230356E-8	40.000004	4
>>END_MODULE