##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934561.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	447741
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95315595400019	33.0	31.0	34.0	30.0	34.0
2	32.0763008078331	33.0	31.0	34.0	30.0	34.0
3	32.13372463098086	33.0	31.0	34.0	30.0	34.0
4	35.735641810779	37.0	35.0	37.0	33.0	37.0
5	35.79629741301333	37.0	35.0	37.0	35.0	37.0
6	35.825412459435256	37.0	35.0	37.0	35.0	37.0
7	36.06265899258723	37.0	35.0	37.0	35.0	37.0
8	35.86356174663477	37.0	35.0	37.0	35.0	37.0
9	37.62912710696586	39.0	38.0	39.0	35.0	39.0
10	36.93163234995232	39.0	37.0	39.0	32.0	39.0
11	36.95407389539935	39.0	37.0	39.0	33.0	39.0
12	36.94103957421813	39.0	37.0	39.0	33.0	39.0
13	36.951366973317164	39.0	37.0	39.0	33.0	39.0
14	38.08193799540359	40.0	37.0	41.0	33.0	41.0
15	38.255763934953464	40.0	37.0	41.0	34.0	41.0
16	38.36794262754584	40.0	37.0	41.0	34.0	41.0
17	38.38297140534372	40.0	37.0	41.0	34.0	41.0
18	38.392586338977225	40.0	37.0	41.0	34.0	41.0
19	38.362015093547384	40.0	37.0	41.0	34.0	41.0
20	38.251428839440656	40.0	37.0	41.0	34.0	41.0
21	38.240288470343344	40.0	37.0	41.0	34.0	41.0
22	38.24957062230173	40.0	37.0	41.0	34.0	41.0
23	38.264769141088266	40.0	37.0	41.0	34.0	41.0
24	38.18032969953612	40.0	37.0	41.0	34.0	41.0
25	38.098612814104584	40.0	37.0	41.0	34.0	41.0
26	37.981761777456164	40.0	37.0	41.0	34.0	41.0
27	37.93286967242223	40.0	36.0	41.0	34.0	41.0
28	37.980035332926846	40.0	36.0	41.0	34.0	41.0
29	38.00105194744283	40.0	36.0	41.0	34.0	41.0
30	37.85084457309025	40.0	36.0	41.0	34.0	41.0
31	37.92345798128829	40.0	36.0	41.0	34.0	41.0
32	37.861051366749976	40.0	36.0	41.0	34.0	41.0
33	37.813244710669785	40.0	36.0	41.0	34.0	41.0
34	37.84475623183939	40.0	36.0	41.0	34.0	41.0
35	37.823697182076245	40.0	36.0	41.0	34.0	41.0
36	37.73128214749152	40.0	36.0	41.0	33.0	41.0
37	37.72005467446582	40.0	36.0	41.0	33.0	41.0
38	37.643378649710435	40.0	36.0	41.0	33.0	41.0
39	37.58056778360704	40.0	36.0	41.0	33.0	41.0
40	37.533158678789746	40.0	36.0	41.0	33.0	41.0
41	37.46879334257975	40.0	36.0	41.0	33.0	41.0
42	37.50101956264894	40.0	36.0	41.0	33.0	41.0
43	37.45566521716796	40.0	36.0	41.0	33.0	41.0
44	37.37643637728061	39.0	35.0	41.0	33.0	41.0
45	37.27901845039878	39.0	35.0	41.0	33.0	41.0
46	37.21489655850146	39.0	35.0	41.0	32.0	41.0
47	37.1951105661532	39.0	35.0	41.0	32.0	41.0
48	37.16203564114075	39.0	35.0	41.0	32.0	41.0
49	37.16750085428853	39.0	35.0	41.0	32.0	41.0
50	37.05210601664802	39.0	35.0	41.0	32.0	41.0
51	36.02386424294402	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	6.0
11	8.0
12	1.0
13	7.0
14	1.0
15	11.0
16	17.0
17	20.0
18	58.0
19	102.0
20	171.0
21	283.0
22	429.0
23	675.0
24	1041.0
25	1693.0
26	2296.0
27	2826.0
28	3388.0
29	4340.0
30	5681.0
31	7362.0
32	9819.0
33	14263.0
34	26054.0
35	37492.0
36	30834.0
37	46874.0
38	91719.0
39	160164.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13364199392059	22.013396137499132	25.5759468085344	9.277015060045875
2	33.04142350153325	23.070257135263468	26.05658181850668	17.831737544696598
3	31.518444815194496	23.499746505234054	27.17664900020324	17.805159679368206
4	27.734114141881133	25.507157039449147	26.45792991930603	20.300798899363695
5	23.66837077685537	30.538860635948012	26.342014691529254	19.45075389566736
6	24.974483015850684	35.10042636256229	25.22574434773675	14.699346273850283
7	82.40880330369566	4.617178234738387	8.37761116359681	4.596407297969138
8	82.42332062509352	3.3354104270102582	8.060016840092823	6.181252107803395
9	77.58413904467092	5.4388586258573595	10.489323068470387	6.487679261001338
10	38.62590202818147	22.47839710904295	22.47549364476338	16.420207218012198
11	28.332674470285273	24.95728557357937	25.260362575685498	21.44967738044986
12	25.206983501622588	21.565145921414388	32.003100006476956	21.22477057048606
13	22.397323452621045	26.83493358883819	32.940918968778824	17.826823989761937
14	19.953053216033375	29.616452368668494	29.887814607105444	20.54267980819268
15	17.95926662959166	27.512557483009147	36.12021235491054	18.40796353248865
16	20.44284530565662	25.58465720137312	32.33208484369312	21.64041264927715
17	20.32134649272682	26.457259889087663	28.154223088794637	25.067170529390875
18	21.25045506218997	25.484376012024807	32.67380025505817	20.59136867072705
19	21.275022836863275	28.9448587464628	28.35121197299332	21.42890644368061
20	21.55978567966749	28.58728595326316	29.663801170766135	20.189127196303218
21	20.294098597180067	28.06064220162996	31.343566928201795	20.301692272988177
22	19.084917396441245	25.940219903917665	27.96572125402856	27.009141445612528
23	18.016442541558625	27.67760826013253	29.383728539490466	24.92222065881838
24	20.35127450914703	25.81179744539812	30.95249262408401	22.88443542137084
25	17.750217201462455	28.49325837928624	28.94753886733625	24.808985551915057
26	17.95680985212433	29.070601084108894	29.97089835418244	23.001690709584334
27	19.77504852135498	29.0951688587822	28.604483395534473	22.52529922432835
28	16.73556810745498	26.71298808909615	30.7519302453874	25.79951355806147
29	19.107028393647223	24.69195360710768	29.53828217652616	26.662735822718936
30	20.412470602424168	28.158466613510935	28.085209976303265	23.343852807761632
31	20.500914591248065	28.420895115703054	25.03232895803601	26.045861335012876
32	21.366146946560622	28.665679488811612	25.436803866521046	24.531369698106715
33	20.576404662516946	28.75256007379266	26.870668533817543	23.800366729872852
34	19.652209647988457	25.003070971834163	27.755778452274864	27.588940927902517
35	19.185421929195673	28.352775376836163	26.268981397727703	26.19282129624046
36	20.368918638230586	29.605731885174684	26.596849517913256	23.42849995868147
37	20.274444377441423	28.746529801827396	27.96817803149589	23.010847789235296
38	19.905257727123495	29.275630330927925	26.850344283860533	23.968767658088048
39	22.240089694711898	25.843512209067292	29.508577503512072	22.40782059270873
40	22.43618520528609	24.63098085723666	29.55972314351377	23.373110793963477
41	18.25832345038761	29.21577429808751	26.06975907946782	26.45614317205706
42	19.685041128688237	28.26723485229184	26.679933264990254	25.367790754029674
43	21.008797496767105	26.49902510603228	27.88911446572907	24.60306293147154
44	20.402196805742605	24.9747063592568	27.06341389329992	27.559682941700668
45	21.910658170683497	23.515157200256397	26.886525915652133	27.687658713407977
46	22.76539338590837	26.135868727679618	27.787493215944036	23.31124467046797
47	19.138743157316394	26.919804083164152	29.438894360802337	24.502558398717113
48	19.294413511382697	25.862496398587574	28.548424200598117	26.294665889431613
49	21.47134169084359	22.918606962507344	30.743443195954807	24.866608150694265
50	20.70505046444261	22.492691087034693	29.625162761507212	27.177095687015484
51	19.635682235935505	23.417556131781545	26.71946504787366	30.22729658440929
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	148.0
1	235.5
2	323.0
3	618.5
4	914.0
5	681.5
6	449.0
7	451.5
8	454.0
9	514.0
10	574.0
11	559.0
12	544.0
13	561.5
14	579.0
15	581.0
16	583.0
17	609.5
18	636.0
19	662.0
20	688.0
21	917.0
22	1146.0
23	1184.0
24	1222.0
25	1610.5
26	2376.0
27	2753.0
28	3363.0
29	3973.0
30	4891.5
31	5810.0
32	6725.5
33	7641.0
34	8512.0
35	9383.0
36	11132.0
37	12881.0
38	14705.0
39	16529.0
40	18498.0
41	20467.0
42	24285.5
43	28104.0
44	31962.5
45	35821.0
46	42861.0
47	49901.0
48	56684.0
49	63467.0
50	62977.0
51	62487.0
52	52157.0
53	41827.0
54	34246.5
55	26666.0
56	21925.5
57	17185.0
58	14577.5
59	11970.0
60	10113.0
61	8256.0
62	6943.0
63	5630.0
64	4317.0
65	3004.0
66	2374.0
67	1744.0
68	1373.5
69	1003.0
70	765.0
71	527.0
72	359.0
73	191.0
74	124.0
75	90.5
76	124.0
77	96.0
78	68.0
79	37.0
80	6.0
81	3.0
82	0.0
83	0.0
84	0.0
85	2.5
86	5.0
87	3.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	447741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.04297946723658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.57781323426866	26.46947320246504
2	13.570419089724572	10.325183495843742
3	5.040063827775019	5.752171341408205
4	2.5464268127990257	3.87494651816536
5	1.5039220705577867	2.86068382252769
6	1.071168320067848	2.445026064377726
7	0.7442671214805838	1.9819897174437586
8	0.6339701910965927	1.9294491970182177
9	0.4980466590527425	1.7052460941662333
>10	4.663947166113055	35.01890080026116
>50	0.09601894128217196	2.450113042119456
>100	0.05097301821152337	3.4580281257080765
>500	5.927095140874811E-4	0.15919195727372862
>1k	0.0023708380563499244	1.569596621221565
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	2776	0.6200012953917555	No Hit
CCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	1797	0.401348100799346	No Hit
CTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGCT	1327	0.2963766999224998	No Hit
TCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	1061	0.2369673538943273	No Hit
ACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	706	0.15768044472139026	No Hit
GCCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTG	476	0.1063114613135719	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCC	461	0.10296131022175765	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31000064769587776	0.0
2	0.0	0.0	0.0	1.7918841473083769	0.0
3	0.0	0.0	0.0	2.4898322914363438	0.0
4	0.0	0.0	0.0	3.4973343964479464	0.0
5	0.0	0.0	0.0	6.061316698716445	0.0
6	0.0	0.0	0.0	7.392666742603425	0.0
7	0.0	0.0	0.0	8.635796141072628	0.0
8	0.0	0.0	0.0	10.695692375726145	0.0
9	0.0	0.0	0.0	11.375549703958315	0.0
10	0.0	0.0	0.0	13.292059471882181	0.0
11	0.0	0.0	0.0	16.094125845075613	0.0
12	0.0	0.0	0.0	17.93581557194896	0.0
13	0.0	0.0	0.0	18.720420957651857	0.0
14	0.0	0.0	0.0	18.97101225931956	0.0
15	0.0	0.0	0.0	19.548354964142217	0.0
16	0.0	0.0	0.0	20.91141977169837	0.0
17	0.0	0.0	0.0	22.657741864158073	0.0
18	0.0	0.0	0.0	24.40518067364838	0.0
19	0.0	0.0	0.0	25.47745236643506	0.0
20	0.0	0.0	0.0	26.43983910341023	0.0
21	0.0	0.0	0.0	27.746398029217783	0.0
22	0.0	0.0	0.0	29.144527751534927	0.0
23	2.2334340612094938E-4	0.0	0.0	30.61747751490259	0.0
24	2.2334340612094938E-4	0.0	0.0	31.614259136420387	0.0
25	2.2334340612094938E-4	0.0	0.0	32.564808672871145	0.0
26	2.2334340612094938E-4	0.0	0.0	33.41731045403481	0.0
27	4.4668681224189875E-4	0.0	0.0	34.2530614797394	0.0
28	4.4668681224189875E-4	0.0	0.0	35.17435302998832	0.0
29	4.4668681224189875E-4	0.0	0.0	36.041372132549846	0.0
30	4.4668681224189875E-4	0.0	0.0	36.98343461956801	0.0
31	6.700302183628482E-4	0.0	0.0	37.895122403353724	0.0
32	6.700302183628482E-4	0.0	0.0	38.7786689179682	0.0
33	6.700302183628482E-4	0.0	0.0	39.59856256183821	0.0
34	6.700302183628482E-4	0.0	0.0	40.39455846125327	0.0
35	6.700302183628482E-4	0.0	0.0	41.22204578093139	0.0
36	6.700302183628482E-4	0.0	0.0	42.00397104576083	0.0
37	6.700302183628482E-4	0.0	0.0	42.76289193975982	0.0
38	6.700302183628482E-4	0.0	0.0	43.569608322668685	0.0
39	6.700302183628482E-4	0.0	0.0	44.3479600930002	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACACG	25	3.8869766E-5	45.000004	2
GCGAATG	30	2.162522E-6	45.000004	1
GTCCTCG	25	3.8869766E-5	45.000004	9
CGCATGG	35	1.2097007E-7	45.000004	2
CACGCCG	35	1.2097007E-7	45.000004	26
TTGAGCG	25	3.8869766E-5	45.000004	1
AACGGGC	25	3.8869766E-5	45.000004	4
CATAGCG	35	1.2097007E-7	45.000004	22
CTACGCG	30	2.162522E-6	45.000004	1
AGCGATC	25	3.8869766E-5	45.000004	17
GGACGGC	25	3.8869766E-5	45.000004	8
GTATCAC	30	2.162522E-6	45.000004	9
TTCGGCG	25	3.8869766E-5	45.000004	1
GGTTGCG	50	2.1827873E-11	45.000004	1
CGGTAGT	35	1.2097007E-7	45.000004	12
CGCCGGT	35	1.2097007E-7	45.000004	28
TACACGG	25	3.8869766E-5	45.000004	3
GCGTAAG	25	3.8869766E-5	45.000004	1
TGCAACG	25	3.8869766E-5	45.000004	1
CGGGTTC	20	7.028669E-4	45.0	6
>>END_MODULE