##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934560.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	597771
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.031580655468396	33.0	31.0	34.0	30.0	34.0
2	32.142698458105194	33.0	31.0	34.0	30.0	34.0
3	32.20622278431038	34.0	31.0	34.0	30.0	34.0
4	35.79490641064889	37.0	35.0	37.0	35.0	37.0
5	35.84088221074626	37.0	35.0	37.0	35.0	37.0
6	35.87114965429905	37.0	35.0	37.0	35.0	37.0
7	36.10176974125543	37.0	35.0	37.0	35.0	37.0
8	35.89803955026256	37.0	35.0	37.0	35.0	37.0
9	37.6407370046389	39.0	38.0	39.0	35.0	39.0
10	37.11701136388349	39.0	37.0	39.0	33.0	39.0
11	37.07925944885249	39.0	37.0	39.0	34.0	39.0
12	37.1350333154335	39.0	37.0	39.0	34.0	39.0
13	37.168507672670636	39.0	37.0	39.0	34.0	39.0
14	38.355791766412224	40.0	38.0	41.0	34.0	41.0
15	38.506933257049944	40.0	38.0	41.0	34.0	41.0
16	38.58949330094635	40.0	38.0	41.0	35.0	41.0
17	38.58324341595695	40.0	38.0	41.0	34.0	41.0
18	38.56561961018517	40.0	38.0	41.0	35.0	41.0
19	38.53745330569733	40.0	38.0	41.0	34.0	41.0
20	38.427807638711144	40.0	38.0	41.0	34.0	41.0
21	38.4271869997039	40.0	38.0	41.0	34.0	41.0
22	38.42101406725987	40.0	38.0	41.0	34.0	41.0
23	38.44830210900161	40.0	38.0	41.0	34.0	41.0
24	38.35846670380464	40.0	37.0	41.0	34.0	41.0
25	38.27936283292431	40.0	37.0	41.0	34.0	41.0
26	38.15870626042414	40.0	37.0	41.0	34.0	41.0
27	38.10701087874788	40.0	37.0	41.0	34.0	41.0
28	38.118194091048245	40.0	37.0	41.0	34.0	41.0
29	38.136814934147026	40.0	37.0	41.0	34.0	41.0
30	38.024306967049256	40.0	37.0	41.0	34.0	41.0
31	38.01028989362147	40.0	37.0	41.0	34.0	41.0
32	37.95955809164379	40.0	37.0	41.0	34.0	41.0
33	37.9258261106678	40.0	37.0	41.0	33.0	41.0
34	37.8671347388883	40.0	37.0	41.0	33.0	41.0
35	37.883349978503475	40.0	37.0	41.0	33.0	41.0
36	37.78424179158909	40.0	37.0	41.0	33.0	41.0
37	37.735010564246174	40.0	37.0	41.0	33.0	41.0
38	37.67918985698537	40.0	37.0	41.0	33.0	41.0
39	37.67046410749267	40.0	37.0	41.0	33.0	41.0
40	37.6304287093218	40.0	36.0	41.0	33.0	41.0
41	37.574132903737386	40.0	36.0	41.0	33.0	41.0
42	37.556724899668936	40.0	36.0	41.0	33.0	41.0
43	37.488934725839826	40.0	36.0	41.0	33.0	41.0
44	37.389105527032925	40.0	36.0	41.0	33.0	41.0
45	37.292961685996815	40.0	36.0	41.0	32.0	41.0
46	37.23792221435968	39.0	35.0	41.0	32.0	41.0
47	37.18796495647999	39.0	35.0	41.0	32.0	41.0
48	37.14199584790831	39.0	35.0	41.0	32.0	41.0
49	37.117434602883044	39.0	35.0	41.0	32.0	41.0
50	37.00040483730392	39.0	35.0	41.0	32.0	41.0
51	36.04857545782582	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	8.0
9	5.0
10	6.0
11	6.0
12	6.0
13	9.0
14	7.0
15	7.0
16	17.0
17	41.0
18	77.0
19	138.0
20	230.0
21	398.0
22	603.0
23	967.0
24	1511.0
25	2230.0
26	3264.0
27	4027.0
28	4826.0
29	5829.0
30	7125.0
31	9426.0
32	12557.0
33	17549.0
34	30717.0
35	43504.0
36	40477.0
37	61696.0
38	122271.0
39	228038.0
40	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80301319401577	21.306654220428893	25.232572339574855	10.657760245980484
2	33.07972450988756	23.460656338296772	25.361384209003113	18.09823494281255
3	31.054701549590057	23.759767536397717	27.478248359321544	17.707282554690675
4	27.88325295138105	25.88934558551686	26.443236624058375	19.784164839043715
5	24.614108078177093	30.149672700749953	26.060146778615888	19.176072442457063
6	25.591405404410715	34.443290156263856	24.70143248836093	15.2638719509645
7	82.86802136604152	4.374250340013148	8.010592685158697	4.7471356087866425
8	83.36520172440616	3.3213387735437148	8.110129129716897	5.203330372333218
9	77.15780792310099	6.193174309225439	10.429913796420369	6.2191039712532055
10	35.85085258401629	29.434014028783594	19.25486515739305	15.460268229807067
11	24.65408994414249	24.622807061567052	29.169364187958262	21.55373880633219
12	24.26079552203101	22.156812558655407	32.16934244049979	21.413049478813793
13	21.731566101400034	25.699473544216765	33.53407910387088	19.03488125051232
14	19.532061608876976	28.379429580893017	31.030277480841324	21.05823132938868
15	17.933790699113874	27.3758680163474	34.76732728753988	19.92301399699885
16	20.517054189647876	26.940918846849378	31.831754969712485	20.710271993790265
17	20.372684523002956	26.213884581219226	29.063638082141825	24.34979281363599
18	20.589824531467734	25.346662852497026	32.99992806609889	21.063584549936344
19	20.420194355363506	28.328406697548058	29.497248946502925	21.754150000585508
20	21.22802879363502	28.532163654643668	29.60782640844069	20.63198114328062
21	20.393595540767283	28.02812448245231	31.46221546378128	20.116064512999124
22	19.654349240762766	25.951074909957157	29.09860130384378	25.295974545436295
23	18.08953595942259	27.57259887147419	29.66570810561235	24.672157063490868
24	19.97688077875976	26.359257976716837	30.891261034744073	22.77260020977933
25	18.621512251347088	28.47996975430391	28.787278071368466	24.11123992298054
26	19.99394416925545	28.68606874538912	28.751645697098056	22.568341388257377
27	21.21715506439757	27.435757171224434	29.516152506561877	21.83093525781612
28	18.575173435981338	26.324294755014883	31.332065289216104	23.76846651978768
29	20.532946563148766	24.59453536554968	30.865164084574193	24.00735398672736
30	22.77293478606356	26.75706917866541	28.814713326675268	21.655282708595767
31	21.205779470733777	27.393098694985202	28.163795165707267	23.23732666857375
32	21.417900834935118	27.752266336105297	28.258480254144146	22.57135257481544
33	20.909010306622434	27.329529200981646	27.4068163226386	24.354644169757314
34	19.09242837139975	25.547910487460918	29.787661161213908	25.571999979925426
35	20.822856913433405	26.35975984114318	27.837917864867983	24.97946538055543
36	22.463786299435736	26.822980706658573	27.496984631238387	23.216248362667308
37	19.02785514854351	26.143790849673206	31.83392971555997	22.99442428622332
38	17.624809500628167	28.761348409340705	29.732456074316083	23.881386015715048
39	19.088580744131114	26.362603739559127	29.67691641113403	24.871899105175725
40	20.478410628819397	26.281970855059882	28.860382989472555	24.379235526648166
41	19.468659403015536	26.47518865920227	27.030919867307045	27.02523207047515
42	21.337100662293754	24.73873774405249	28.780753833825994	25.143407759827763
43	21.979487128013904	24.014881953122515	30.040098967664875	23.965531951198706
44	20.033758747078732	25.01275572083624	29.3654258905166	25.588059641568428
45	21.070777940047275	24.25678060662026	28.654116710245226	26.018324743087234
46	22.717060546597274	25.721722867118007	27.813995660545594	23.74722092573912
47	18.933002771964517	25.672038288909967	30.791724590185876	24.603234348939644
48	19.478362115258186	23.995643816779335	30.83354662571453	25.69244744224795
49	20.77083030123576	22.75520224299941	31.636864284148945	24.837103171615887
50	19.57053788156334	22.58189172776866	31.40533749546231	26.44223289520569
51	19.07904532003058	22.93771360604646	28.101396688698514	29.881844385224444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	169.0
1	332.0
2	495.0
3	1601.0
4	2707.0
5	1808.5
6	910.0
7	917.0
8	924.0
9	975.5
10	1027.0
11	1067.0
12	1107.0
13	1087.0
14	1067.0
15	1093.0
16	1119.0
17	1104.0
18	1089.0
19	1183.5
20	1278.0
21	1420.5
22	1563.0
23	1878.0
24	2193.0
25	2451.5
26	3481.5
27	4253.0
28	5637.5
29	7022.0
30	7716.0
31	8410.0
32	10077.5
33	11745.0
34	13394.5
35	15044.0
36	17182.0
37	19320.0
38	20753.5
39	22187.0
40	25312.0
41	28437.0
42	33169.0
43	37901.0
44	41927.5
45	45954.0
46	54280.0
47	62606.0
48	71112.0
49	79618.0
50	77909.5
51	76201.0
52	64134.0
53	52067.0
54	43341.5
55	34616.0
56	29072.0
57	23528.0
58	20230.5
59	16933.0
60	14566.0
61	12199.0
62	10138.0
63	8077.0
64	6893.0
65	5709.0
66	4444.0
67	3179.0
68	2523.0
69	1867.0
70	1453.0
71	1039.0
72	811.0
73	583.0
74	478.5
75	298.5
76	223.0
77	193.5
78	164.0
79	120.0
80	76.0
81	50.0
82	24.0
83	20.5
84	17.0
85	23.5
86	30.0
87	18.0
88	6.0
89	4.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	597771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.64446938191698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.4001747264904	26.878216913548904
2	12.359358091741724	9.305229545294383
3	4.57551394441221	5.167283837608788
4	2.3180243146139117	3.4904318135209
5	1.4427754520891103	2.7156258165574965
6	0.9775282637547317	2.2079119676924077
7	0.7132545135811106	1.8795061388612742
8	0.5856002218520028	1.7635687697241207
9	0.48676251908206575	1.6491525071264643
>10	5.007420153619681	37.960576476670305
>50	0.0878631159696855	2.1949713187369477
>100	0.04169015195868453	2.884665158592991
>500	0.0017931248154272916	0.43487668852779565
>1k	0.0022414060192841147	1.4679830475371727
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	2524	0.42223527069730715	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	1977	0.33072865696060866	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGCT	1599	0.2674937392412814	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1583	0.2648171289674474	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	1016	0.16996475238845646	TruSeq Adapter, Index 13 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG	780	0.13048475084940556	No Hit
ACTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	634	0.10606068210067066	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCC	623	0.10422051253740981	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.691525684584899E-4	0.0	0.0	0.2782001803366172	0.0
2	6.691525684584899E-4	0.0	0.0	1.3535283578494106	0.0
3	6.691525684584899E-4	0.0	0.0	1.9825317722003912	0.0
4	6.691525684584899E-4	0.0	0.0	2.805422143262219	0.0
5	6.691525684584899E-4	0.0	0.0	4.93633849751828	0.0
6	6.691525684584899E-4	0.0	0.0	6.253732616670932	0.0
7	6.691525684584899E-4	0.0	0.0	7.477445376239396	0.0
8	6.691525684584899E-4	0.0	0.0	9.409623417663285	0.0
9	6.691525684584899E-4	0.0	0.0	10.092159037490946	0.0
10	6.691525684584899E-4	0.0	0.0	11.753664864973375	0.0
11	6.691525684584899E-4	0.0	0.0	14.199082926404927	0.0
12	6.691525684584899E-4	0.0	0.0	15.946742147076389	0.0
13	6.691525684584899E-4	0.0	0.0	16.712419973535017	0.0
14	6.691525684584899E-4	0.0	0.0	17.02023015502592	0.0
15	6.691525684584899E-4	0.0	0.0	17.520923564374986	0.0
16	6.691525684584899E-4	0.0	0.0	18.65848293075442	0.0
17	6.691525684584899E-4	0.0	0.0	20.144001632732266	0.0
18	6.691525684584899E-4	0.0	0.0	21.588702028034145	0.0
19	6.691525684584899E-4	0.0	0.0	22.528192234149866	0.0
20	6.691525684584899E-4	0.0	0.0	23.393741081450923	0.0
21	6.691525684584899E-4	0.0	0.0	24.517248243892727	0.0
22	6.691525684584899E-4	0.0	0.0	25.697633374653506	0.0
23	6.691525684584899E-4	0.0	0.0	26.95982240690833	0.0
24	6.691525684584899E-4	0.0	0.0	27.95970363232743	0.0
25	6.691525684584899E-4	0.0	0.0	28.827761801760207	0.0
26	6.691525684584899E-4	0.0	0.0	29.601804035324562	0.0
27	6.691525684584899E-4	0.0	0.0	30.34205406418177	0.0
28	6.691525684584899E-4	0.0	0.0	31.138680196931602	0.0
29	6.691525684584899E-4	0.0	0.0	31.924432600443982	0.0
30	6.691525684584899E-4	0.0	0.0	32.78830856632389	0.0
31	8.364407105731124E-4	0.0	0.0	33.5857711397843	0.0
32	8.364407105731124E-4	0.0	0.0	34.395278459476955	0.0
33	8.364407105731124E-4	0.0	0.0	35.15459933653523	0.0
34	8.364407105731124E-4	0.0	0.0	35.92261919698346	0.0
35	8.364407105731124E-4	0.0	0.0	36.704022108800864	0.0
36	8.364407105731124E-4	0.0	0.0	37.412989255082636	0.0
37	8.364407105731124E-4	0.0	0.0	38.13082267289648	0.0
38	8.364407105731124E-4	0.0	0.0	38.82339558125101	0.0
39	8.364407105731124E-4	0.0	0.0	39.528347812122036	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTC	25	3.8885504E-5	45.000004	6
GATCGAT	25	3.8885504E-5	45.000004	9
GAATGCG	30	2.163746E-6	45.000004	1
CAACCGG	25	3.8885504E-5	45.000004	2
CACGACC	50	2.1827873E-11	45.000004	27
TCGCTAC	25	3.8885504E-5	45.000004	11
GCGATCA	50	2.1827873E-11	45.000004	9
TCACGAC	20	7.0305663E-4	45.0	25
CGACGGT	20	7.0305663E-4	45.0	28
ATGTACG	20	7.0305663E-4	45.0	1
AAAGTCG	20	7.0305663E-4	45.0	1
GTCGTAG	20	7.0305663E-4	45.0	1
GTATCGC	20	7.0305663E-4	45.0	9
CGGTCTA	20	7.0305663E-4	45.0	31
CCGCGTA	20	7.0305663E-4	45.0	2
TATACGG	40	6.8048394E-9	45.0	2
TCGACGG	20	7.0305663E-4	45.0	2
GTAACGT	20	7.0305663E-4	45.0	35
TCAACGG	55	1.8189894E-12	45.0	2
AGCCGCG	85	0.0	42.35294	1
>>END_MODULE