##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934557.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	300436
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01438908785898	33.0	31.0	34.0	30.0	34.0
2	32.14579810675152	33.0	31.0	34.0	30.0	34.0
3	32.19367186355829	34.0	31.0	34.0	30.0	34.0
4	35.78642040234859	37.0	35.0	37.0	33.0	37.0
5	35.833854797694016	37.0	35.0	37.0	35.0	37.0
6	35.84536806507875	37.0	35.0	37.0	35.0	37.0
7	36.03875367798799	37.0	35.0	37.0	35.0	37.0
8	35.80458067608409	37.0	35.0	37.0	35.0	37.0
9	37.52594562569066	39.0	37.0	39.0	35.0	39.0
10	37.226953494255014	39.0	37.0	39.0	34.0	39.0
11	37.12645621696468	39.0	37.0	39.0	34.0	39.0
12	36.80630483697027	39.0	35.0	39.0	33.0	39.0
13	36.650737594695705	39.0	35.0	39.0	33.0	39.0
14	37.681579437883606	40.0	36.0	41.0	33.0	41.0
15	37.903839752892466	40.0	36.0	41.0	33.0	41.0
16	38.06286530242714	40.0	36.0	41.0	34.0	41.0
17	38.08128187034843	40.0	36.0	41.0	34.0	41.0
18	38.060428843414236	40.0	36.0	41.0	34.0	41.0
19	38.04180923724188	40.0	36.0	41.0	34.0	41.0
20	37.91999960058049	40.0	36.0	41.0	34.0	41.0
21	37.88811260967394	40.0	35.0	41.0	34.0	41.0
22	37.867106471927464	40.0	35.0	41.0	34.0	41.0
23	37.889983224380565	40.0	35.0	41.0	34.0	41.0
24	37.80307619592858	40.0	35.0	41.0	34.0	41.0
25	37.71187873623667	39.0	35.0	41.0	34.0	41.0
26	37.57075716625172	39.0	35.0	41.0	33.0	41.0
27	37.50485294705029	39.0	35.0	41.0	33.0	41.0
28	37.544069285971055	39.0	35.0	41.0	33.0	41.0
29	37.59002915762425	39.0	35.0	41.0	33.0	41.0
30	37.478727582579985	39.0	35.0	41.0	33.0	41.0
31	37.467826758444396	39.0	35.0	41.0	33.0	41.0
32	37.403137440253495	39.0	35.0	41.0	33.0	41.0
33	37.373344073280165	39.0	35.0	41.0	33.0	41.0
34	37.298988802939725	39.0	35.0	41.0	33.0	41.0
35	37.268473152351916	39.0	35.0	41.0	33.0	41.0
36	37.17687960164561	39.0	35.0	41.0	33.0	41.0
37	37.11601805376186	39.0	35.0	41.0	33.0	41.0
38	37.04239838101959	39.0	35.0	41.0	32.0	41.0
39	37.01028172389461	39.0	35.0	41.0	32.0	41.0
40	36.88280365868271	39.0	35.0	41.0	32.0	41.0
41	36.81014924975702	39.0	35.0	41.0	32.0	41.0
42	36.811810169220735	39.0	35.0	41.0	32.0	41.0
43	36.73172988589916	39.0	35.0	41.0	32.0	41.0
44	36.62823363378556	38.0	35.0	41.0	32.0	41.0
45	36.53102491046346	38.0	35.0	41.0	32.0	41.0
46	36.477735690796045	38.0	35.0	40.0	31.0	41.0
47	36.44593856927931	38.0	35.0	40.0	31.0	41.0
48	36.413479077074655	38.0	35.0	40.0	31.0	41.0
49	36.40313411175758	38.0	35.0	40.0	31.0	41.0
50	36.24868857260781	38.0	35.0	40.0	31.0	41.0
51	35.26237534782782	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	5.0
11	9.0
12	4.0
13	3.0
14	7.0
15	5.0
16	10.0
17	37.0
18	63.0
19	106.0
20	173.0
21	271.0
22	419.0
23	637.0
24	943.0
25	1326.0
26	1832.0
27	2290.0
28	2818.0
29	3463.0
30	4258.0
31	5492.0
32	7412.0
33	11057.0
34	23099.0
35	32463.0
36	21757.0
37	31408.0
38	56790.0
39	92177.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.31369076941512	20.463260062043165	22.33886751254843	10.88418165599329
2	33.933350197712656	23.162004553382417	22.846795989828117	20.057849259076807
3	33.32989388755009	23.862985793979416	24.978697626116713	17.82842269235378
4	30.466721697799198	25.486626103396397	22.86010997350517	21.18654222529923
5	24.97769907734093	29.72346855902755	24.03540188259729	21.263430481034227
6	27.206792794472033	33.485001797387795	24.42982864903008	14.878376759110093
7	80.12488516689078	5.922725638738367	8.826838328296208	5.125550866074638
8	79.06908626130024	3.8813590914537537	10.39788840218882	6.651666245057183
9	72.75093530735332	6.747859777123913	12.541439774194837	7.959765141327936
10	44.03167396716772	22.353180045001263	19.36618780705375	14.248958180777272
11	34.676270486892385	21.541027040700847	24.007442516875475	19.775259955531293
12	30.863811260967395	20.571436179419244	29.77572594495999	18.78902661465337
13	24.389886697998907	26.813031727223102	29.99241102930408	18.80467054547391
14	20.119093583991265	28.7608675391764	30.111238333621802	21.008800543210533
15	17.342795137733162	23.984475895032553	37.674579610965395	20.99814935626889
16	21.12762784752826	23.537126043483468	33.35053056224953	21.98471554673874
17	21.36428390738793	23.97815175278595	26.50980574897815	28.147758590847964
18	22.544568560358947	23.38068673527806	31.88033391471062	22.194410789652373
19	23.802074318656885	24.98901596346643	27.675445019904405	23.53346469797228
20	25.477306314822457	26.404292428337484	27.60787655274335	20.510524704096714
21	23.861987245203636	26.538763663475752	29.460850230997615	20.138398860322997
22	21.530708703351127	25.15344366187807	26.732814975568843	26.58303265920196
23	20.23359384361395	26.531773822045295	28.680317937930205	24.55431439641055
24	22.73462567734892	23.384015231197328	30.99561969937025	22.885739392083504
25	20.21295716891451	25.5585216152525	28.868377957368622	25.360143258464362
26	19.817864703297875	26.200588478078522	27.42381072840805	26.557736090215556
27	21.48444261007336	25.85642200002663	29.413252739352142	23.24588265054787
28	16.713709408992266	26.239531880333917	30.64413053029597	26.402628180377853
29	18.54671211173095	23.69356535168888	31.054201227549292	26.70552130903088
30	21.665845637673247	26.046479117016602	29.685190855956012	22.60248438935414
31	23.414304544062627	27.713389873384013	25.410070697253325	23.46223488530003
32	22.90737461555872	25.618101692207322	26.873943202545636	24.60058048968832
33	24.366254376972137	26.214235311347505	26.080096925801172	23.33941338587919
34	21.317684964518232	24.301015856954557	28.679652238746357	25.701646939780854
35	20.079151632960098	23.347401776085423	29.158289951936517	27.41515663901796
36	24.994008707345326	25.273269514971574	27.072654408925693	22.660067368757407
37	21.828941937717182	26.31675298566084	29.56037225898361	22.293932817638364
38	20.451277476733814	28.030595534489876	26.893914178061216	24.624212810715093
39	24.09298486200056	26.1107190882584	27.56593750416062	22.230358545580422
40	25.38643837622655	22.51993769055639	27.727036706652996	24.36658722656406
41	20.440293440200243	22.562209588731044	26.552077647152807	30.445419323915907
42	22.80652118920502	21.898174652837877	28.109148038184507	27.186156119772598
43	23.197619459718545	22.70500206366747	29.09538137906243	25.00199709755156
44	20.515517447975608	24.26773089776192	28.39473298805736	26.822018666205118
45	21.3689438016749	23.093104687853653	26.85397222703005	28.683979283441396
46	23.15368331358426	24.15090069099575	27.97301255508661	24.72240344033338
47	20.02889134457921	22.949646513733374	31.256573779440544	25.764888362246868
48	20.79111691009067	21.190869269994277	30.829527752998974	27.188486066916084
49	22.992251261499955	20.371060725079552	32.274760681143405	24.36192733227709
50	21.456483244351542	22.600820141394507	30.173148357720116	25.769548256533838
51	19.514638725052926	22.508620804430894	26.66890785391897	31.307832616597214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	139.0
1	313.0
2	487.0
3	736.5
4	986.0
5	668.0
6	350.0
7	359.5
8	369.0
9	410.5
10	452.0
11	490.5
12	529.0
13	560.0
14	591.0
15	605.5
16	620.0
17	615.0
18	610.0
19	575.0
20	540.0
21	549.0
22	558.0
23	661.0
24	764.0
25	946.5
26	1397.5
27	1666.0
28	1867.5
29	2069.0
30	2761.5
31	3454.0
32	3682.5
33	3911.0
34	4765.5
35	5620.0
36	6156.5
37	6693.0
38	7555.5
39	8418.0
40	10122.0
41	11826.0
42	13854.5
43	15883.0
44	18585.0
45	21287.0
46	23890.5
47	26494.0
48	30864.0
49	35234.0
50	36424.5
51	37615.0
52	31689.0
53	25763.0
54	22623.0
55	19483.0
56	16674.0
57	13865.0
58	12657.5
59	11450.0
60	11137.5
61	10825.0
62	9821.0
63	8817.0
64	7879.5
65	6942.0
66	5870.5
67	4799.0
68	4225.0
69	3651.0
70	2975.5
71	2300.0
72	1990.5
73	1681.0
74	1366.0
75	951.5
76	852.0
77	578.5
78	305.0
79	271.0
80	237.0
81	171.5
82	106.0
83	57.0
84	8.0
85	5.5
86	3.0
87	2.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	300436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.343993396264096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.02637563932367	22.3258863784633
2	12.884237391044836	8.334553781837064
3	4.991098350364814	4.8429615625291245
4	2.475996418758297	3.2033444726996763
5	1.3831002438949092	2.23674925774541
6	0.9827832834223498	1.9072281617382738
7	0.704928323711319	1.5960137932870895
8	0.5289535158943328	1.368677522001358
9	0.47029524662200406	1.3690103715932844
>10	6.377285871589845	45.284519831178685
>50	0.11525835365790908	2.5016975329188247
>100	0.05454189949883198	3.118134977166518
>500	0.0020581848867483768	0.3994195103116803
>1k	0.003087277330122565	1.5118028465297102
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	1881	0.6260900824135589	No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	1580	0.5259023552437124	No Hit
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT	1081	0.3598104088724387	No Hit
TCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	642	0.21368943801674897	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	558	0.18573007229493135	No Hit
ACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	472	0.15710500738926092	No Hit
GCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	375	0.12481859697240011	No Hit
GGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	341	0.11350171084690251	No Hit
CGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	337	0.1121703124791969	No Hit
GGCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT	303	0.1008534263536993	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCC	302	0.10052057676177288	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.656991838528006E-4	0.0	0.0	0.3707944454060099	0.0
2	9.985487757792008E-4	0.0	0.0	1.9578212997110866	0.0
3	9.985487757792008E-4	0.0	0.0	2.725705308285292	0.0
4	9.985487757792008E-4	0.0	0.0	3.646700129145642	0.0
5	9.985487757792008E-4	0.0	0.0	6.245256893315049	0.0
6	9.985487757792008E-4	0.0	0.0	7.4321985381245925	0.0
7	9.985487757792008E-4	0.0	0.0	8.63445126416275	0.0
8	9.985487757792008E-4	0.0	0.0	10.415196580968992	0.0
9	9.985487757792008E-4	0.0	0.0	11.027306980521642	0.0
10	9.985487757792008E-4	0.0	0.0	12.422945319469038	0.0
11	9.985487757792008E-4	0.0	0.0	14.856075836451025	0.0
12	0.0013313983677056011	0.0	0.0	16.556604401603003	0.0
13	0.0013313983677056011	0.0	0.0	17.2336204715813	0.0
14	0.0013313983677056011	0.0	0.0	17.519871120638005	0.0
15	0.0013313983677056011	0.0	0.0	18.120997483657085	0.0
16	0.0013313983677056011	0.0	0.0	19.305609181323145	0.0
17	0.0013313983677056011	0.0	0.0	20.6200322198405	0.0
18	0.0013313983677056011	0.0	0.0	21.94310934774794	0.0
19	0.0013313983677056011	0.0	0.0	22.752599555312944	0.0
20	0.0013313983677056011	0.0	0.0	23.532798998788426	0.0
21	0.0013313983677056011	0.0	0.0	24.528019278648365	0.0
22	0.0013313983677056011	0.0	0.0	25.485960404212545	0.0
23	0.0013313983677056011	0.0	0.0	26.53210667163722	0.0
24	0.0013313983677056011	0.0	0.0	27.32062735491086	0.0
25	0.0013313983677056011	0.0	0.0	28.026601339386758	0.0
26	0.0013313983677056011	0.0	0.0	28.721591287329083	0.0
27	0.0013313983677056011	0.0	0.0	29.357666857500433	0.0
28	0.0013313983677056011	0.0	0.0	29.97576854970776	0.0
29	0.0013313983677056011	0.0	0.0	30.660440160300364	0.0
30	0.0013313983677056011	0.0	0.0	31.477585908479675	0.0
31	0.0013313983677056011	0.0	0.0	32.18788693765062	0.0
32	0.0013313983677056011	0.0	0.0	32.814975568839955	0.0
33	0.0013313983677056011	0.0	0.0	33.48533464697972	0.0
34	0.0013313983677056011	0.0	0.0	34.16168501777417	0.0
35	0.0013313983677056011	0.0	0.0	34.94654435553662	0.0
36	0.0013313983677056011	0.0	0.0	35.56331464937624	0.0
37	0.0013313983677056011	0.0	0.0	36.19473032526062	0.0
38	0.0013313983677056011	0.0	0.0	36.881066183812855	0.0
39	0.0013313983677056011	0.0	0.0	37.627647818503775	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAAAT	20	7.0249586E-4	45.000004	19
CTCACGT	20	7.0249586E-4	45.000004	14
ATTAGGT	20	7.0249586E-4	45.000004	24
AGTCAAG	20	7.0249586E-4	45.000004	30
TGCTTAA	20	7.0249586E-4	45.000004	9
TAGACGG	30	2.16013E-6	45.000004	2
GGGTCGA	20	7.0249586E-4	45.000004	7
CGCATTG	30	2.16013E-6	45.000004	27
CGTGACC	20	7.0249586E-4	45.000004	18
TTAGACG	20	7.0249586E-4	45.000004	1
CGGCAGG	60	0.0	45.000004	2
TTCGTAG	20	7.0249586E-4	45.000004	1
GTCTAGT	20	7.0249586E-4	45.000004	10
GCGTTAG	20	7.0249586E-4	45.000004	1
ATCCGAG	20	7.0249586E-4	45.000004	31
ACGATAA	20	7.0249586E-4	45.000004	28
GCTTAAA	20	7.0249586E-4	45.000004	10
ACGTGAC	20	7.0249586E-4	45.000004	17
TCATGCA	40	6.7866495E-9	45.000004	24
CGGTGTC	20	7.0249586E-4	45.000004	11
>>END_MODULE