FastQCFastQC Report
Sat 14 Jan 2017
SRR2934555.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934555.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166559
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30030.25742375653524596No Hit
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC19350.16587245051471894Illumina PCR Primer Index 5 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15520.13304084919836887No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC14140.12121118606088506No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC12700.1088671897435106No Hit
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC12210.10466680210773738Illumina PCR Primer Index 5 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG207.033331E-445.01
TTGGCCG302.1655305E-644.9999961
ATAGCGG1650.042.2727282
CGAATAT2300.041.08695614
CGACAGG2350.040.2127652
GCTACGA2450.039.48979610
CCAACGG800.039.3752
TACGAAT2400.039.37499612
AATTGCG356.2483687E-638.571431
TATACGG1050.038.571432
TCGACGT356.2483687E-638.5714325
ACGGGAG8550.038.4210555
CGCACGG2050.038.4146352
ACACGAC7850.038.40764226
GACACGA7900.038.1645625
ACGCTCG659.094947E-1238.076921
CACGACC8100.037.77777527
AGACACG8350.037.72455224
CGAGACA8000.037.68749622
TATTGCG601.5643309E-1037.4999961
Produced by FastQC (version 0.11.5)