##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934555.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1166559
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24482173640596	33.0	31.0	34.0	30.0	34.0
2	32.3566754874807	34.0	31.0	34.0	30.0	34.0
3	32.411734854387994	34.0	31.0	34.0	30.0	34.0
4	35.944190563872034	37.0	35.0	37.0	35.0	37.0
5	35.97091017256735	37.0	35.0	37.0	35.0	37.0
6	35.989835919143395	37.0	35.0	37.0	35.0	37.0
7	36.182283965063064	37.0	36.0	37.0	35.0	37.0
8	35.95553761104239	37.0	36.0	37.0	35.0	37.0
9	37.69450580725021	39.0	38.0	39.0	35.0	39.0
10	37.30788241314841	39.0	37.0	39.0	34.0	39.0
11	37.18295345541888	39.0	37.0	39.0	34.0	39.0
12	37.171778709863794	39.0	37.0	39.0	34.0	39.0
13	37.202456969600334	39.0	37.0	39.0	34.0	39.0
14	38.45745907408026	40.0	38.0	41.0	34.0	41.0
15	38.58549460421633	40.0	38.0	41.0	34.0	41.0
16	38.65649572803433	40.0	38.0	41.0	35.0	41.0
17	38.60876475171852	40.0	38.0	41.0	34.0	41.0
18	38.59859724197405	40.0	38.0	41.0	35.0	41.0
19	38.5819097019525	40.0	38.0	41.0	34.0	41.0
20	38.473251674368804	40.0	38.0	41.0	34.0	41.0
21	38.47256932568349	40.0	38.0	41.0	34.0	41.0
22	38.450585011131025	40.0	38.0	41.0	34.0	41.0
23	38.438631908030366	40.0	38.0	41.0	34.0	41.0
24	38.350698078708405	40.0	37.0	41.0	34.0	41.0
25	38.26390264015794	40.0	37.0	41.0	34.0	41.0
26	38.12674369663257	40.0	37.0	41.0	34.0	41.0
27	38.110500197589666	40.0	37.0	41.0	34.0	41.0
28	38.121564361511076	40.0	37.0	41.0	34.0	41.0
29	38.13792958607323	40.0	37.0	41.0	34.0	41.0
30	38.034561475244715	40.0	37.0	41.0	34.0	41.0
31	38.06921553046181	40.0	37.0	41.0	34.0	41.0
32	38.01158964098687	40.0	37.0	41.0	34.0	41.0
33	37.985080908895306	40.0	37.0	41.0	34.0	41.0
34	37.9188373669913	40.0	37.0	41.0	34.0	41.0
35	37.88875573374343	40.0	37.0	41.0	34.0	41.0
36	37.82678887223021	40.0	37.0	41.0	33.0	41.0
37	37.804683689380475	40.0	36.0	41.0	33.0	41.0
38	37.73723146450372	40.0	36.0	41.0	33.0	41.0
39	37.659209692780216	40.0	36.0	41.0	33.0	41.0
40	37.542564928134794	40.0	36.0	41.0	33.0	41.0
41	37.47361599370456	40.0	35.0	41.0	33.0	41.0
42	37.482496813277336	40.0	35.0	41.0	33.0	41.0
43	37.45923952410465	40.0	35.0	41.0	33.0	41.0
44	37.37774771786082	40.0	35.0	41.0	33.0	41.0
45	37.238165407836206	39.0	35.0	41.0	33.0	41.0
46	37.15792085955361	39.0	35.0	41.0	33.0	41.0
47	37.11618015033959	39.0	35.0	41.0	33.0	41.0
48	37.090338336937954	39.0	35.0	41.0	33.0	41.0
49	37.06874577282418	39.0	35.0	41.0	33.0	41.0
50	36.94956620282386	39.0	35.0	41.0	32.0	41.0
51	36.07462288662639	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	16.0
10	13.0
11	16.0
12	25.0
13	15.0
14	28.0
15	38.0
16	63.0
17	111.0
18	185.0
19	319.0
20	565.0
21	905.0
22	1455.0
23	2125.0
24	3028.0
25	4382.0
26	6055.0
27	7611.0
28	8758.0
29	10813.0
30	13619.0
31	17327.0
32	23160.0
33	32867.0
34	62877.0
35	83820.0
36	78642.0
37	118640.0
38	228641.0
39	459917.0
40	510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.18434129778262	20.795947740320038	23.747191526532305	10.272519435365036
2	33.02996247939453	23.37764313678091	24.424225435661633	19.16816894816293
3	33.09108240560486	24.967446995822755	24.695021854874035	17.24644874369835
4	28.998361848822046	26.071205999867985	25.108031398326187	19.822400752983775
5	25.978883194077625	30.58242232068845	23.110189883237798	20.328504601996126
6	27.472935359463175	34.942510408817725	22.601600090522638	14.982954141196458
7	85.16860270247797	4.6898613786358	6.633869354228976	3.5076665646572525
8	85.61555823580291	4.44469589622128	6.20405826023373	3.735687607742086
9	79.30503300733182	6.988587803960194	8.2797355298789	5.426643658829086
10	38.71437278354545	33.56178298740141	14.808252304426953	12.915591924626188
11	27.602461598598953	25.730117379403872	27.61677720543925	19.050643816557926
12	26.72286613878938	23.87397465537534	28.517031714641096	20.886127491194188
13	23.84397188654839	25.82098290785121	30.194015047674398	20.141030157926004
14	20.35456414977725	27.29849068928361	29.573214899546446	22.773730261392693
15	20.332447823041953	27.030694546953903	30.923853829939162	21.71300380006498
16	23.261661004715577	27.712614621292193	28.97273091202417	20.05299346196806
17	22.298572125370427	26.457298773572536	28.514545770938287	22.729583330118754
18	23.786623736990585	25.236614693298836	28.810458793768685	22.166302775941894
19	22.65680518516423	27.52299712230586	27.151648566424846	22.668549126105066
20	25.416888472850495	26.622999779693956	28.53871943039315	19.421392317062402
21	24.39713722152073	27.800136984070246	27.992754759939274	19.809971034469754
22	24.503347023168136	24.617186100317255	27.712700343488844	23.16676653302576
23	22.61951602962216	27.80793770396525	28.326471271491627	21.24607499492096
24	22.225451091629314	25.418517194586816	30.29482435093296	22.061207362850915
25	21.437321215643614	28.437652960544646	27.793450652731664	22.331575171080072
26	21.774980948241797	27.797993929154035	27.57271599636195	22.85430912624222
27	22.031633205007207	27.446704367288753	27.84951296933974	22.672149458364302
28	18.929432630497043	28.57146530951285	28.671674557394866	23.82742750259524
29	20.71639754183029	26.02448740269459	28.361445927724187	24.89766912775093
30	21.551503181579328	27.20171032926753	28.44656806899608	22.80021842015706
31	21.724061963432625	28.295439836304894	26.311142428286953	23.66935577197553
32	21.51421402603726	27.54648500418753	27.316663794973078	23.622637174802133
33	21.59967905609575	27.256143924139288	26.702292811593754	24.44188420817121
34	20.092082783639746	27.193738164979226	28.541633985079194	24.172545066301833
35	20.00541764282818	27.964980768225185	28.293125336995384	23.736476251951252
36	20.533894985165773	29.795835444242424	25.421088860486268	24.249180710105534
37	20.31564627249886	28.52380376817632	27.575459106654698	23.585090852670117
38	20.314274717352486	28.013928142511435	28.05198879782334	23.619808342312734
39	22.520764059083167	26.307627818224365	27.53405528567351	23.63755283701896
40	23.21948568396455	26.841591381147463	27.939178387034	21.99974454785399
41	19.292123244516564	27.70772845608323	27.405900601684095	25.594247697716106
42	20.25992684467738	26.898425197525373	27.26668775432704	25.574960203470205
43	21.474010315809146	25.93876520604616	27.584974270482675	25.00225020766202
44	21.05388583003517	26.077463720223328	27.231541653701186	25.63710879604032
45	21.93673873331739	25.251273188925722	27.022465216075652	25.789522861681235
46	21.68540125274418	27.180536946695366	26.229877785864236	24.904184014696213
47	19.511572067936555	27.257086868302416	29.479777705199652	23.751563358561377
48	20.398539636657897	26.161300028545494	28.997504626855562	24.442655707941046
49	21.973685000072866	25.88158850088165	28.426594797176996	23.718131701868487
50	20.021962026781328	25.913648602428168	29.31621975399444	24.748169616796066
51	20.05633662763735	25.212698200433927	27.11590241042245	27.615062761506277
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	275.0
1	829.5
2	1384.0
3	3210.5
4	5037.0
5	3328.0
6	1619.0
7	1521.0
8	1423.0
9	1468.5
10	1514.0
11	1533.0
12	1552.0
13	1575.5
14	1599.0
15	1633.0
16	1667.0
17	1682.0
18	1697.0
19	1902.5
20	2108.0
21	2496.5
22	2885.0
23	3402.0
24	3919.0
25	4959.0
26	8016.5
27	10034.0
28	12331.5
29	14629.0
30	17753.5
31	20878.0
32	23930.5
33	26983.0
34	30877.5
35	34772.0
36	38172.0
37	41572.0
38	44990.0
39	48408.0
40	52529.0
41	56650.0
42	61736.5
43	66823.0
44	73463.0
45	80103.0
46	90156.0
47	100209.0
48	105063.0
49	109917.0
50	110578.0
51	111239.0
52	101027.0
53	90815.0
54	82355.5
55	73896.0
56	66327.5
57	58759.0
58	52324.5
59	45890.0
60	43162.0
61	40434.0
62	36097.0
63	31760.0
64	27419.0
65	23078.0
66	18966.5
67	14855.0
68	12726.5
69	10598.0
70	8905.0
71	7212.0
72	6074.5
73	4937.0
74	4539.5
75	3648.0
76	3154.0
77	2195.5
78	1237.0
79	888.5
80	540.0
81	392.0
82	244.0
83	161.0
84	78.0
85	47.5
86	17.0
87	14.5
88	12.0
89	7.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1166559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.42134166924501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.32943683323991	19.285275168098565
2	11.162822376332425	6.1219913274900986
3	4.124879162342296	3.393291625648118
4	1.9976397978977687	2.1911185372094506
5	1.208680352480745	1.6571818457139003
6	0.8497984569952547	1.3981568303558456
7	0.6362842334305038	1.2213437154556048
8	0.5294941458695188	1.1615551908602504
9	0.4843664571389927	1.1953780302897035
>10	8.379274357782979	54.13516372570646
>50	0.23956382566555	4.0496804136551665
>100	0.05176306084802837	2.5252561444449073
>500	0.0034719126177056446	0.5743427965214807
>1k	0.0025250273583313776	1.0902646485504959
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3003	0.25742375653524596	No Hit
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	1935	0.16587245051471894	Illumina PCR Primer Index 5 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1552	0.13304084919836887	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC	1414	0.12121118606088506	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC	1270	0.1088671897435106	No Hit
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	1221	0.10466680210773738	Illumina PCR Primer Index 5 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.2861098324216776E-4	0.0	0.0	0.10183796961833906	0.0
2	4.2861098324216776E-4	0.0	0.0	0.47318652549935325	0.0
3	5.143331798906013E-4	0.0	0.0	0.7223809511563496	0.0
4	5.143331798906013E-4	0.0	0.0	1.0898720081881843	0.0
5	5.143331798906013E-4	0.0	0.0	2.2466073297621465	0.0
6	5.143331798906013E-4	0.0	0.0	2.944643177070341	0.0
7	5.143331798906013E-4	0.0	0.0	3.5444413870194307	0.0
8	5.143331798906013E-4	0.0	0.0	4.485499661825934	0.0
9	5.143331798906013E-4	0.0	0.0	4.805929232897779	0.0
10	5.143331798906013E-4	0.0	0.0	5.662122533022333	0.0
11	5.143331798906013E-4	0.0	0.0	6.878606225660254	0.0
12	5.143331798906013E-4	0.0	0.0	7.89998619872634	0.0
13	5.143331798906013E-4	0.0	0.0	8.283850195318026	0.0
14	5.143331798906013E-4	0.0	0.0	8.434806983615916	0.0
15	6.000553765390349E-4	0.0	0.0	8.759779831110128	0.0
16	6.000553765390349E-4	0.0	0.0	9.414525969110864	0.0
17	6.000553765390349E-4	0.0	0.0	10.231629947563732	0.0
18	6.000553765390349E-4	0.0	0.0	11.008787382378431	0.0
19	6.000553765390349E-4	0.0	0.0	11.555694996995436	0.0
20	6.000553765390349E-4	0.0	0.0	12.070799676655874	0.0
21	6.000553765390349E-4	0.0	0.0	12.720659649447649	0.0
22	6.000553765390349E-4	0.0	0.0	13.418524052362546	0.0
23	6.000553765390349E-4	0.0	0.0	14.103530125780178	0.0
24	6.000553765390349E-4	0.0	0.0	14.646665963744654	0.0
25	6.000553765390349E-4	0.0	0.0	15.156027256229647	0.0
26	6.000553765390349E-4	0.0	0.0	15.624584783109984	0.0
27	6.000553765390349E-4	0.0	0.0	16.09382808756351	0.0
28	6.857775731874685E-4	0.0	0.0	16.58184455308304	0.0
29	6.857775731874685E-4	0.0	0.0	17.073547073058457	0.0
30	6.857775731874685E-4	0.0	0.0	17.680374503132718	0.0
31	7.71499769835902E-4	0.0	0.0	18.192564628107107	0.0
32	7.71499769835902E-4	0.0	0.0	18.701154420822263	0.0
33	8.572219664843355E-4	0.0	0.0	19.212144435043577	0.0
34	0.0010286663597812026	0.0	0.0	19.711476230520702	0.0
35	0.0011143885564296363	0.0	0.0	20.265155898672933	0.0
36	0.0011143885564296363	0.0	0.0	20.754801085928786	0.0
37	0.0011143885564296363	0.0	0.0	21.262105045694216	0.0
38	0.0012001107530780698	0.0	0.0	21.77429517066861	0.0
39	0.0012001107530780698	0.0	0.0	22.300800902483285	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	20	7.033331E-4	45.0	1
TTGGCCG	30	2.1655305E-6	44.999996	1
ATAGCGG	165	0.0	42.272728	2
CGAATAT	230	0.0	41.086956	14
CGACAGG	235	0.0	40.212765	2
GCTACGA	245	0.0	39.489796	10
CCAACGG	80	0.0	39.375	2
TACGAAT	240	0.0	39.374996	12
AATTGCG	35	6.2483687E-6	38.57143	1
TATACGG	105	0.0	38.57143	2
TCGACGT	35	6.2483687E-6	38.57143	25
ACGGGAG	855	0.0	38.421055	5
CGCACGG	205	0.0	38.414635	2
ACACGAC	785	0.0	38.407642	26
GACACGA	790	0.0	38.16456	25
ACGCTCG	65	9.094947E-12	38.07692	1
CACGACC	810	0.0	37.777775	27
AGACACG	835	0.0	37.724552	24
CGAGACA	800	0.0	37.687496	22
TATTGCG	60	1.5643309E-10	37.499996	1
>>END_MODULE