##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934554.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	882615
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.188549934002936	33.0	31.0	34.0	30.0	34.0
2	32.3118018615138	34.0	31.0	34.0	30.0	34.0
3	32.371042866935184	34.0	31.0	34.0	30.0	34.0
4	35.90981911705557	37.0	35.0	37.0	35.0	37.0
5	35.93029463582649	37.0	35.0	37.0	35.0	37.0
6	35.94540088260453	37.0	35.0	37.0	35.0	37.0
7	36.14703806302862	37.0	35.0	37.0	35.0	37.0
8	35.9340607173003	37.0	35.0	37.0	35.0	37.0
9	37.687644103034735	39.0	38.0	39.0	35.0	39.0
10	37.297032114795236	39.0	37.0	39.0	34.0	39.0
11	37.176088101833756	39.0	37.0	39.0	34.0	39.0
12	36.9987672994454	39.0	35.0	39.0	34.0	39.0
13	36.92481319714712	39.0	35.0	39.0	33.0	39.0
14	37.93939826538185	40.0	37.0	41.0	33.0	41.0
15	38.15916679412881	40.0	37.0	41.0	33.0	41.0
16	38.30516023407714	40.0	37.0	41.0	34.0	41.0
17	38.3174373877625	40.0	37.0	41.0	34.0	41.0
18	38.280860850993925	40.0	37.0	41.0	34.0	41.0
19	38.25864391609026	40.0	37.0	41.0	34.0	41.0
20	38.144117197192436	40.0	36.0	41.0	34.0	41.0
21	38.131914821298075	40.0	36.0	41.0	34.0	41.0
22	38.11728216719634	40.0	36.0	41.0	34.0	41.0
23	38.155518544325666	40.0	36.0	41.0	34.0	41.0
24	38.0717447584734	40.0	36.0	41.0	34.0	41.0
25	37.98082629459051	40.0	36.0	41.0	34.0	41.0
26	37.82184077995502	40.0	35.0	41.0	34.0	41.0
27	37.79717883788515	40.0	35.0	41.0	34.0	41.0
28	37.81508132084771	40.0	35.0	41.0	34.0	41.0
29	37.7986993196354	40.0	35.0	41.0	34.0	41.0
30	37.69941367413878	40.0	35.0	41.0	33.0	41.0
31	37.704588070676344	40.0	35.0	41.0	33.0	41.0
32	37.65717895118483	40.0	35.0	41.0	33.0	41.0
33	37.61741982631158	40.0	35.0	41.0	33.0	41.0
34	37.54073293565145	40.0	35.0	41.0	33.0	41.0
35	37.51292466137557	40.0	35.0	41.0	33.0	41.0
36	37.44724823394119	40.0	35.0	41.0	33.0	41.0
37	37.41301700061749	40.0	35.0	41.0	33.0	41.0
38	37.34765554630275	40.0	35.0	41.0	33.0	41.0
39	37.28021051081162	39.0	35.0	41.0	33.0	41.0
40	37.16516827835466	39.0	35.0	41.0	33.0	41.0
41	37.07113067418977	39.0	35.0	41.0	32.0	41.0
42	37.086354752638464	39.0	35.0	41.0	33.0	41.0
43	37.04961846331639	39.0	35.0	41.0	33.0	41.0
44	36.974129150309025	39.0	35.0	41.0	32.0	41.0
45	36.85108456121865	39.0	35.0	41.0	32.0	41.0
46	36.82304288959512	39.0	35.0	41.0	32.0	41.0
47	36.7922944885369	39.0	35.0	41.0	32.0	41.0
48	36.72522674099126	39.0	35.0	41.0	32.0	41.0
49	36.698058609926186	39.0	35.0	41.0	32.0	41.0
50	36.5752689451233	38.0	35.0	41.0	32.0	41.0
51	35.66763877794961	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	14.0
9	8.0
10	17.0
11	16.0
12	18.0
13	14.0
14	22.0
15	37.0
16	38.0
17	82.0
18	164.0
19	291.0
20	474.0
21	710.0
22	1169.0
23	1641.0
24	2550.0
25	3597.0
26	5083.0
27	6479.0
28	7698.0
29	8997.0
30	11246.0
31	14908.0
32	20339.0
33	30407.0
34	64034.0
35	72659.0
36	60820.0
37	91946.0
38	167778.0
39	309009.0
40	346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.586501475728376	21.50382669680438	24.29938308322428	10.61028874424296
2	34.072047268627884	23.052859967256392	24.739212453901192	18.13588031021453
3	33.38420489114733	23.437852291202844	25.876288075774827	17.301654741874998
4	29.545045121598886	25.255292511457426	25.4343060111147	19.765356355828985
5	26.024597361250372	29.464035848019805	24.821014825263564	19.690351965466256
6	26.620553695552424	33.994890184281935	24.711680630852637	14.67287548931301
7	83.91461735864448	4.472618299031854	7.314174356882673	4.298589985440991
8	84.72131110393546	3.875982166629844	7.136293854058678	4.266412875376013
9	78.95027843397177	5.643004027803742	9.7701715923704	5.636545945854081
10	45.32667131195368	23.8927505197623	16.713289486355887	14.067288681928133
11	32.006254142519666	24.92525053392476	24.107340119984364	18.961155203571206
12	30.116981923035524	21.227602068852217	29.09943746707228	19.555978541039977
13	26.080680704497432	22.53111492553378	31.56653807152609	19.821666298442697
14	21.33931555661302	23.832475088232126	32.18674053806019	22.641468817094655
15	20.42679990709426	24.849566345462065	33.3886235787971	21.33501016864658
16	25.624422879738052	23.848676942947943	29.688369221008028	20.838530956305977
17	25.268548574406736	24.39614101278587	26.90266990703761	23.432640505769786
18	24.99832882967092	23.684845600856548	29.975357318876295	21.34146825059624
19	24.435002804167162	26.31067906165202	26.383304158664878	22.87101397551594
20	24.498224027463845	28.182389830220423	26.817128646125433	20.5022574961903
21	25.60663482945565	26.36608260679911	28.2145669402854	19.81271562345983
22	22.9213190349133	23.410773666887604	29.345411079576035	24.32249621862307
23	21.861627096752265	24.723010599185375	29.779122267353266	23.636240036709097
24	23.916203554211066	24.4070177823853	29.529862964033015	22.14691569937062
25	23.469689502217843	25.42682823201509	27.038063028613834	24.065419237153233
26	21.061278133727615	26.69895707641497	27.452286670858754	24.787478118998656
27	22.405465576723714	25.22345530044244	29.217495737099412	23.153583385734436
28	19.831749970258834	25.730811282382465	28.194739495703107	26.242699251655594
29	21.316089121530904	25.42331594183194	28.198591684936243	25.062003251700908
30	23.16559315216714	24.976122091738752	28.403097613342172	23.455187142751935
31	23.055012661239612	25.332562895486703	27.587793092118307	24.024631351155374
32	23.784662621867973	25.464670326246434	25.66815655750242	25.082510494383172
33	22.636370331344924	24.169315046764446	27.559808070336445	25.634506551554185
34	19.84840502370796	25.692515989417807	28.417260073758094	26.04181891311614
35	20.10400910929454	26.74450354911258	27.395070330778427	25.756417010814452
36	21.96019782124709	26.011567897667724	26.844887068540647	25.183347212544543
37	19.915818335287753	26.518017482141136	28.21513343870204	25.351030743869067
38	20.51222786832311	24.859650017278202	27.773264673725233	26.854857440673452
39	21.00134260124743	25.369385292568108	27.033191142230756	26.596080963953707
40	22.781280626320648	24.93646720257417	27.86379112070382	24.418461050401362
41	19.18594177529274	24.070517722902967	28.379078080476766	28.36446242132753
42	19.382290126499097	23.855928122680897	30.09375548795341	26.668026262866597
43	19.72955365589753	23.627515961092886	30.334517315024108	26.308413067985477
44	20.89427440050305	23.907932677328166	27.978450400231132	27.21934252193765
45	20.60105482005178	24.391269126402793	26.790616520226827	28.217059533318604
46	21.506319289837585	24.49051964899758	27.706304560878753	26.296856500286083
47	18.542172974626535	24.41857435008469	31.07130515570209	25.96794751958668
48	19.194439251542292	23.851282835664474	30.538570044696723	26.41570786809651
49	21.450462545957183	23.271981554811553	29.931170442378612	25.346385456852648
50	20.521518442355955	23.44374387473587	29.153821315069422	26.880916367838754
51	20.083388566928956	23.731185171337447	27.00237362836571	29.18305263336789
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	255.0
1	650.5
2	1046.0
3	2643.0
4	4240.0
5	2781.0
6	1322.0
7	1242.0
8	1162.0
9	1180.0
10	1198.0
11	1226.0
12	1254.0
13	1264.0
14	1274.0
15	1285.0
16	1296.0
17	1346.0
18	1396.0
19	1406.5
20	1417.0
21	1803.5
22	2190.0
23	2283.5
24	2377.0
25	2950.0
26	4705.5
27	5888.0
28	6929.0
29	7970.0
30	9873.0
31	11776.0
32	12940.5
33	14105.0
34	16765.0
35	19425.0
36	21424.5
37	23424.0
38	26446.0
39	29468.0
40	34361.0
41	39254.0
42	42474.5
43	45695.0
44	49542.5
45	53390.0
46	60769.0
47	68148.0
48	77507.0
49	86866.0
50	88525.5
51	90185.0
52	81119.0
53	72053.0
54	64050.5
55	56048.0
56	53096.5
57	50145.0
58	49300.5
59	48456.0
60	42456.0
61	36456.0
62	32702.5
63	28949.0
64	26062.0
65	23175.0
66	20073.5
67	16972.0
68	14287.5
69	11603.0
70	9654.5
71	7706.0
72	6343.0
73	4980.0
74	4062.5
75	2466.5
76	1788.0
77	1330.0
78	872.0
79	679.0
80	486.0
81	343.5
82	201.0
83	112.0
84	23.0
85	13.5
86	4.0
87	5.5
88	7.0
89	3.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	882615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.015365352073974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.89553611707287	21.98850954502171
2	11.263140405715731	6.986608293899602
3	4.137482128906978	3.849765595971802
4	2.100112969015528	2.6054308405858166
5	1.365391231404149	2.117405394525893
6	0.9411302248193358	1.7513698659990733
7	0.7136313166078634	1.5493475207892122
8	0.5942175228245656	1.474389885520661
9	0.5163851670708877	1.4414287157185806
>10	7.291343430171435	49.17831294079452
>50	0.13585295402268416	2.6850941935311528
>100	0.04208487653192287	2.5529631214495603
>500	0.0011074967508400498	0.2362094864578364
>1k	0.0025841590852934498	1.5831645997346118
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	3254	0.3686771695473111	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2541	0.28789449533488554	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2218	0.2512986976201401	No Hit
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	1932	0.218894988188508	No Hit
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	1488	0.16858992879114904	No Hit
GAGCGCCATTATTCCTGTCAGTTTAAGTTATGTTGGTGGTTGTTAAACAGG	1251	0.14173790384255877	No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT	1143	0.12950153804320116	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.531987333095404E-4	0.0	0.0	0.13539312157622518	0.0
2	4.531987333095404E-4	0.0	0.0	0.6643893430317862	0.0
3	4.531987333095404E-4	0.0	0.0	0.9987367085308997	0.0
4	4.531987333095404E-4	0.0	0.0	1.4557876310735711	0.0
5	4.531987333095404E-4	0.0	0.0	2.799408575653031	0.0
6	4.531987333095404E-4	0.0	0.0	3.8131008423831454	0.0
7	4.531987333095404E-4	0.0	0.0	4.642001325606295	0.0
8	4.531987333095404E-4	0.0	0.0	5.923647343405675	0.0
9	4.531987333095404E-4	0.0	0.0	6.4969437410422435	0.0
10	4.531987333095404E-4	0.0	0.0	7.589265988001563	0.0
11	4.531987333095404E-4	0.0	0.0	9.182826034001234	0.0
12	4.531987333095404E-4	0.0	0.0	10.32216764954142	0.0
13	4.531987333095404E-4	0.0	0.0	10.831563025781342	0.0
14	4.531987333095404E-4	0.0	0.0	11.103255666400413	0.0
15	4.531987333095404E-4	0.0	0.0	11.519632002628553	0.0
16	4.531987333095404E-4	0.0	0.0	12.221976739575013	0.0
17	4.531987333095404E-4	0.0	0.0	13.064019986064139	0.0
18	4.531987333095404E-4	0.0	0.0	13.930762563518636	0.0
19	4.531987333095404E-4	0.0	0.0	14.519241118721073	0.0
20	4.531987333095404E-4	0.0	0.0	15.095596607807481	0.0
21	4.531987333095404E-4	0.0	0.0	15.834083943735378	0.0
22	4.531987333095404E-4	0.0	0.0	16.630580717526893	0.0
23	4.531987333095404E-4	0.0	0.0	17.39603337808671	0.0
24	4.531987333095404E-4	0.0	0.0	18.01249695507101	0.0
25	4.531987333095404E-4	0.0	0.0	18.580694866957845	0.0
26	4.531987333095404E-4	0.0	0.0	19.100740413430543	0.0
27	4.531987333095404E-4	0.0	0.0	19.661800445267755	0.0
28	4.531987333095404E-4	0.0	0.0	20.193062660389863	0.0
29	4.531987333095404E-4	0.0	0.0	20.784147108308833	0.0
30	4.531987333095404E-4	0.0	0.0	21.637860222180677	0.0
31	4.531987333095404E-4	0.0	0.0	22.27075225324745	0.0
32	4.531987333095404E-4	0.0	0.0	22.860703704333147	0.0
33	4.531987333095404E-4	0.0	0.0	23.421877035853687	0.0
34	4.531987333095404E-4	0.0	0.0	24.0272372438719	0.0
35	4.531987333095404E-4	0.0	0.0	24.674518334721256	0.0
36	5.664984166369255E-4	0.0	0.0	25.229913382392095	0.0
37	5.664984166369255E-4	0.0	0.0	25.812840253111492	0.0
38	5.664984166369255E-4	0.0	0.0	26.487879766376054	0.0
39	5.664984166369255E-4	0.0	0.0	27.472227415124376	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCGAT	35	1.2115015E-7	45.000004	14
ACCGGTA	25	3.8900667E-5	45.0	41
TACCCGT	20	7.032396E-4	45.0	36
CGCATAA	20	7.032396E-4	45.0	42
TGAACCG	20	7.032396E-4	45.0	1
AATTGCG	20	7.032396E-4	45.0	1
AACGCAT	20	7.032396E-4	45.0	40
CAATGCG	25	3.8900667E-5	45.0	1
ATAACGG	40	6.8121153E-9	45.0	2
ACCGTAA	20	7.032396E-4	45.0	18
CGGTATT	25	3.8900667E-5	45.0	43
CGGTACA	25	3.8900667E-5	45.0	21
CGATCAT	20	7.032396E-4	45.0	10
GGTTACG	25	3.8900667E-5	45.0	1
CGGACTA	20	7.032396E-4	45.0	11
CGTTTTT	1105	0.0	40.316746	1
CTTACGG	45	1.9274012E-8	40.0	2
CGTATGG	85	0.0	39.705883	2
CTATGCG	40	3.4570076E-7	39.375	1
ATGATCG	40	3.4570076E-7	39.375	27
>>END_MODULE