##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934551.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	692477
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19988100687821	33.0	31.0	34.0	30.0	34.0
2	32.31792391660662	34.0	31.0	34.0	30.0	34.0
3	32.38650669986151	34.0	31.0	34.0	30.0	34.0
4	35.927886413555974	37.0	35.0	37.0	35.0	37.0
5	35.93732355009625	37.0	35.0	37.0	35.0	37.0
6	35.958229659613245	37.0	35.0	37.0	35.0	37.0
7	36.15982047057159	37.0	36.0	37.0	35.0	37.0
8	35.94688632257822	37.0	36.0	37.0	35.0	37.0
9	37.69014999776166	39.0	38.0	39.0	35.0	39.0
10	37.3441760520566	39.0	37.0	39.0	34.0	39.0
11	37.23467638636374	39.0	37.0	39.0	34.0	39.0
12	36.99779198442692	39.0	35.0	39.0	34.0	39.0
13	36.9193012908732	39.0	35.0	39.0	33.0	39.0
14	37.879463144624296	40.0	37.0	41.0	33.0	41.0
15	38.10645263308384	40.0	37.0	41.0	33.0	41.0
16	38.26898221890402	40.0	37.0	41.0	34.0	41.0
17	38.29706112982814	40.0	37.0	41.0	34.0	41.0
18	38.30207068249198	40.0	37.0	41.0	34.0	41.0
19	38.30055871891774	40.0	37.0	41.0	34.0	41.0
20	38.172411502475896	40.0	36.0	41.0	34.0	41.0
21	38.14317154215952	40.0	36.0	41.0	34.0	41.0
22	38.14857099947002	40.0	36.0	41.0	34.0	41.0
23	38.1941147503816	40.0	36.0	41.0	34.0	41.0
24	38.11976859881267	40.0	36.0	41.0	34.0	41.0
25	37.998760969678415	40.0	36.0	41.0	34.0	41.0
26	37.84533060303808	40.0	35.0	41.0	34.0	41.0
27	37.819446710865485	40.0	35.0	41.0	34.0	41.0
28	37.835900686954226	40.0	35.0	41.0	34.0	41.0
29	37.80403681277501	40.0	35.0	41.0	34.0	41.0
30	37.70889430262666	40.0	35.0	41.0	33.0	41.0
31	37.69333277495137	40.0	35.0	41.0	33.0	41.0
32	37.57873113475249	40.0	35.0	41.0	33.0	41.0
33	37.561150767462316	40.0	35.0	41.0	33.0	41.0
34	37.49677173393484	40.0	35.0	41.0	33.0	41.0
35	37.45413349468647	40.0	35.0	41.0	33.0	41.0
36	37.41208877695577	40.0	35.0	41.0	33.0	41.0
37	37.35061236690894	39.0	35.0	41.0	33.0	41.0
38	37.221816753480624	39.0	35.0	41.0	33.0	41.0
39	37.170977519830984	39.0	35.0	41.0	33.0	41.0
40	37.064126317552784	39.0	35.0	41.0	32.0	41.0
41	36.98826242604448	39.0	35.0	41.0	32.0	41.0
42	37.00772733246014	39.0	35.0	41.0	32.0	41.0
43	36.95338473335576	39.0	35.0	41.0	32.0	41.0
44	36.84712849668653	39.0	35.0	41.0	32.0	41.0
45	36.704257325514064	39.0	35.0	41.0	32.0	41.0
46	36.66331589352426	38.0	35.0	41.0	32.0	41.0
47	36.63661753386755	38.0	35.0	41.0	32.0	41.0
48	36.5619146917515	38.0	35.0	41.0	32.0	41.0
49	36.52220940190071	38.0	35.0	41.0	32.0	41.0
50	36.39250256687226	38.0	35.0	40.0	31.0	41.0
51	35.40346177562576	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	13.0
10	16.0
11	6.0
12	7.0
13	7.0
14	16.0
15	34.0
16	40.0
17	79.0
18	118.0
19	204.0
20	354.0
21	575.0
22	836.0
23	1263.0
24	2040.0
25	2800.0
26	3874.0
27	4776.0
28	5790.0
29	7002.0
30	8771.0
31	11707.0
32	15977.0
33	24953.0
34	51775.0
35	58724.0
36	49551.0
37	75166.0
38	135014.0
39	230713.0
40	269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.50042817306568	20.629710445256666	24.08585411501032	9.784007266667341
2	34.09961630494587	22.500241885290055	24.815697849892487	18.584443959871592
3	33.685884152109026	23.622589631135764	25.084732056082732	17.606794160672486
4	29.383214171734224	25.894578448092858	24.6068822502408	20.115325129932113
5	25.897755448917437	29.447043006482527	25.11520238217298	19.539999162427055
6	26.817930415017393	34.09037412072892	23.727141840089995	15.36455362416369
7	84.42533109402912	4.492279165950638	7.153450583918311	3.9289391561019356
8	84.97264169062655	3.668569497615083	7.13814321630899	4.22064559544938
9	79.20147528365563	6.006264467989551	9.281174681613974	5.511085566740845
10	44.48840900130979	26.50297410599919	15.711135532299267	13.297481360391753
11	33.62682081859759	24.350700456477256	23.35644360751332	18.66603511741184
12	30.357253742723582	21.581366601345604	28.29812398101309	19.763255674917723
13	25.926204047210234	23.823462728726007	30.266275991838	19.984057232225762
14	21.671044670075684	24.670855494117493	31.92929151437521	21.728808321431615
15	20.796502988546912	24.61872379876877	33.19633720686752	21.3884360058168
16	26.212278530550474	24.395900513663268	28.94623214922662	20.445588806559638
17	25.515360076941185	23.866929876371344	26.77937317773731	23.83833686895016
18	26.171988383729712	22.887258349374783	29.610658548948198	21.33009471794731
19	25.24632587075094	25.28907097275433	26.389468531084788	23.07513462540994
20	26.007217568236925	27.790959122108028	26.17271042937166	20.02911288028339
21	25.472326156681017	26.34080265481742	28.462317159992317	19.72455402850925
22	23.991699363300153	22.92003922151927	29.397943902829986	23.69031751235059
23	22.86400847970402	25.138019024458576	29.380614807423207	22.6173576884142
24	23.79718026735906	24.037043829614557	29.43635673098168	22.729419172044704
25	23.03542211510274	25.736017225120833	27.430225119390244	23.79833554038618
26	21.810688297228644	26.47770250853097	27.074834254422893	24.636774939817496
27	23.14084077882731	24.515326862841654	28.437767608166048	23.906064750164987
28	19.81379886985416	26.790348271494942	27.845112545254207	25.55074031339669
29	22.55150712586844	26.50109678733012	27.219243382812714	23.728152703988727
30	25.843168798386085	24.638219031101393	27.234117523036865	22.284494647475654
31	26.761899673202144	25.325462073108568	26.141229239382678	21.771409014306613
32	26.709045932211467	25.586409368108974	25.987577926775906	21.71696677290365
33	25.582510321642456	25.094551876813238	25.991476973242435	23.33146082830188
34	22.938956817338337	25.279251152023825	29.243281726324483	22.538510304313355
35	21.89444559169474	28.69799285752451	27.40293179412457	22.004629756656175
36	25.79450292211871	28.468671161641467	23.669089370477288	22.067736545762532
37	24.539732005539534	26.934612990756374	26.407086444748344	22.118568558955747
38	22.825306833295546	27.858687003322853	27.12191163027797	22.194094533103627
39	23.10863754319638	26.003896158283958	26.058193990558532	24.82927230796113
40	26.24794758526276	24.70811304924207	26.67554301442503	22.368396351070142
41	21.75610164669729	25.202569904848826	25.761000004332274	27.28032844412161
42	22.417928682107853	24.35402186643022	27.37015092198008	25.857898529481844
43	22.669778202019707	23.669089370477288	28.71344463426222	24.947687793240785
44	23.405542711165857	24.079788931617944	27.281339308020335	25.233329049195856
45	23.36077588136501	24.20715778285777	26.080144178073784	26.351922157703434
46	23.60006180710695	25.212100907322554	26.859953471378834	24.32788381419166
47	20.974703852979953	25.22293159195179	29.685029250068958	24.117335304999298
48	21.312332395155362	25.51073898483271	29.279961644935497	23.896966975076428
49	24.676776268381477	23.601217080134067	28.743626142095696	22.97838050938876
50	22.729419172044704	24.61655766184292	27.721787149609302	24.932236016503076
51	21.930114646407027	24.188240187038705	25.83291575027041	28.048729416283862
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	219.0
1	486.5
2	754.0
3	1742.0
4	2730.0
5	1783.0
6	836.0
7	836.0
8	836.0
9	826.0
10	816.0
11	799.5
12	783.0
13	841.0
14	899.0
15	913.5
16	928.0
17	1019.0
18	1110.0
19	1122.0
20	1134.0
21	1209.5
22	1285.0
23	1600.0
24	1915.0
25	2218.0
26	3586.0
27	4651.0
28	5487.0
29	6323.0
30	6901.0
31	7479.0
32	9378.0
33	11277.0
34	13880.5
35	16484.0
36	16853.5
37	17223.0
38	18967.0
39	20711.0
40	24003.0
41	27295.0
42	30961.0
43	34627.0
44	37779.0
45	40931.0
46	46717.5
47	52504.0
48	60778.0
49	69052.0
50	69891.0
51	70730.0
52	64640.0
53	58550.0
54	52979.5
55	47409.0
56	44900.5
57	42392.0
58	39756.5
59	37121.0
60	33368.5
61	29616.0
62	27009.5
63	24403.0
64	21334.5
65	18266.0
66	15658.5
67	13051.0
68	11468.5
69	9886.0
70	7931.5
71	5977.0
72	4909.0
73	3841.0
74	3281.5
75	2188.5
76	1655.0
77	1266.0
78	877.0
79	694.0
80	511.0
81	294.5
82	78.0
83	60.0
84	42.0
85	30.0
86	18.0
87	11.0
88	4.0
89	3.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	692477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.861469793534713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54913245034581	22.477990525267728
2	11.464844708188451	7.305736067150254
3	4.281197095059023	4.09215695773175
4	2.1380464702822897	2.7248521212029075
5	1.334914576912842	2.1266170234628845
6	1.00953963877791	1.9299250023778995
7	0.7694477124785624	1.716101453418807
8	0.6537319471872507	1.6663088550700176
9	0.5566009314283338	1.5960711387381432
>10	7.075894724149309	47.757887367365214
>50	0.11399941361419322	2.288424861154379
>100	0.04944552878446021	2.8357329992245743
>500	4.5782897022648343E-4	0.07541532719131198
>1k	0.002746973821358901	1.4067803006441255
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2386	0.3445601803381195	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	1648	0.2379862435864296	TruSeq Adapter, Index 15 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1643	0.23726419794448048	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1610	0.23249869670761628	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	1234	0.17820086443304253	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT	1123	0.1621714511817721	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3322738516947135E-4	0.0	0.0	0.1706915897567717	0.0
2	4.3322738516947135E-4	0.0	0.0	0.7504942402419141	0.0
3	4.3322738516947135E-4	0.0	0.0	1.0707936870105432	0.0
4	4.3322738516947135E-4	0.0	0.0	1.5148517568092514	0.0
5	4.3322738516947135E-4	0.0	0.0	2.79446104347148	0.0
6	4.3322738516947135E-4	0.0	0.0	3.6372327167544913	0.0
7	5.776365135592951E-4	0.0	0.0	4.3822394101175925	0.0
8	5.776365135592951E-4	0.0	0.0	5.551086895304826	0.0
9	5.776365135592951E-4	0.0	0.0	6.041644704445058	0.0
10	5.776365135592951E-4	0.0	0.0	7.115182164895008	0.0
11	5.776365135592951E-4	0.0	0.0	8.707148396264424	0.0
12	5.776365135592951E-4	0.0	0.0	9.87455179016776	0.0
13	5.776365135592951E-4	0.0	0.0	10.388648287235533	0.0
14	5.776365135592951E-4	0.0	0.0	10.663892085946538	0.0
15	5.776365135592951E-4	0.0	0.0	11.07069260062067	0.0
16	5.776365135592951E-4	0.0	0.0	11.80010310811767	0.0
17	5.776365135592951E-4	0.0	0.0	12.692407112438392	0.0
18	5.776365135592951E-4	0.0	0.0	13.552507881128182	0.0
19	5.776365135592951E-4	0.0	0.0	14.162925266831968	0.0
20	5.776365135592951E-4	0.0	0.0	14.76756628740016	0.0
21	5.776365135592951E-4	0.0	0.0	15.515461163331057	0.0
22	5.776365135592951E-4	0.0	0.0	16.304079413467885	0.0
23	5.776365135592951E-4	0.0	0.0	17.08619925282717	0.0
24	5.776365135592951E-4	0.0	0.0	17.710624323984767	0.0
25	5.776365135592951E-4	0.0	0.0	18.297647430889402	0.0
26	5.776365135592951E-4	0.0	0.0	18.828784205107173	0.0
27	5.776365135592951E-4	0.0	0.0	19.458696823143583	0.0
28	5.776365135592951E-4	0.0	0.0	19.99763169029441	0.0
29	5.776365135592951E-4	0.0	0.0	20.583643933300312	0.0
30	5.776365135592951E-4	0.0	0.0	21.467572208174424	0.0
31	5.776365135592951E-4	0.0	0.0	22.111781329921428	0.0
32	5.776365135592951E-4	0.0	0.0	22.735051128051907	0.0
33	5.776365135592951E-4	0.0	0.0	23.333482556099337	0.0
34	5.776365135592951E-4	0.0	0.0	23.932924848045495	0.0
35	5.776365135592951E-4	0.0	0.0	24.613236251889955	0.0
36	5.776365135592951E-4	0.0	0.0	25.193183311503486	0.0
37	7.220456419491189E-4	0.0	0.0	25.804611561106	0.0
38	7.220456419491189E-4	0.0	0.0	26.48463414669368	0.0
39	7.220456419491189E-4	0.0	0.0	27.602216391302527	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	25	3.8891932E-5	45.0	1
TAACGGC	20	7.0313417E-4	45.0	3
GCCGATT	20	7.0313417E-4	45.0	9
AGGCCGT	20	7.0313417E-4	45.0	24
ATATACG	20	7.0313417E-4	45.0	1
GCGATAT	25	3.8891932E-5	45.0	9
TAAGGCG	20	7.0313417E-4	45.0	1
GCCGTCC	20	7.0313417E-4	45.0	32
GCGTACG	45	3.8380676E-10	45.0	1
TACGTAG	20	7.0313417E-4	45.0	1
CGTAAGG	90	0.0	42.5	2
TTGCGAG	70	0.0	41.785717	1
GCGTAAG	60	3.6379788E-12	41.249996	1
TATACGG	55	6.002665E-11	40.909092	2
GTTACGG	50	1.0804797E-9	40.5	2
AGGTACG	45	1.9264917E-8	40.0	1
ATGGGCG	165	0.0	39.545456	5
CAATTGG	80	0.0	39.375	2
CGATAGA	40	3.4555705E-7	39.375	10
CGTTTTT	680	0.0	38.713234	1
>>END_MODULE