SRR2934549.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934549.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	499502
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	2158	0.43203030218097227	RNA PCR Primer, Index 48 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	1878	0.3759744705726904	RNA PCR Primer, Index 4 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT	1412	0.2826815508246213	TruSeq Adapter, Index 4 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	741	0.1483477543633459	RNA PCR Primer, Index 48 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	559	0.11191146381796269	No Hit
ACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	530	0.10610568125853351	TruSeq Adapter, Index 4 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAG	35	1.2100827E-7	45.000004	1
GTTAGCG	35	1.2100827E-7	45.000004	1
ATTAGCG	20	7.029452E-4	45.0	1
TTAGCCG	25	3.887628E-5	45.0	1
CGACAGC	20	7.029452E-4	45.0	13
CGTTAGG	40	6.8012014E-9	45.0	2
CGCTAAG	20	7.029452E-4	45.0	19
TTGCGCG	20	7.029452E-4	45.0	1
ATAACGG	25	3.887628E-5	45.0	2
ATCGCTA	20	7.029452E-4	45.0	17
TAGCACG	30	2.1630276E-6	44.999996	1
TCGCGCG	30	2.1630276E-6	44.999996	1
CGAATGG	65	0.0	41.538464	2
CGCAAGG	65	0.0	41.538464	2
GCTACGA	60	3.6379788E-12	41.249996	10
ACGGGCG	100	0.0	40.5	5
TAACGGG	95	0.0	40.263157	3
TATGCGG	90	0.0	40.000004	2
GCGCAAG	45	1.9244908E-8	40.000004	1
CTAACGG	45	1.9244908E-8	40.000004	2