##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934549.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	499502
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11744697718928	33.0	31.0	34.0	30.0	34.0
2	32.227596686299556	33.0	31.0	34.0	30.0	34.0
3	32.28782667536867	34.0	31.0	34.0	30.0	34.0
4	35.84731792865694	37.0	35.0	37.0	35.0	37.0
5	35.8932176447742	37.0	35.0	37.0	35.0	37.0
6	35.92026658551918	37.0	35.0	37.0	35.0	37.0
7	36.132333804469255	37.0	35.0	37.0	35.0	37.0
8	35.90637074526228	37.0	35.0	37.0	35.0	37.0
9	37.645875291790624	39.0	38.0	39.0	35.0	39.0
10	37.215678816100834	39.0	37.0	39.0	34.0	39.0
11	37.099144748169174	39.0	37.0	39.0	34.0	39.0
12	37.097148760165126	39.0	37.0	39.0	34.0	39.0
13	37.11789342184816	39.0	37.0	39.0	33.0	39.0
14	38.307518288215064	40.0	38.0	41.0	34.0	41.0
15	38.449217420550866	40.0	38.0	41.0	34.0	41.0
16	38.53074662363714	40.0	38.0	41.0	34.0	41.0
17	38.52134325788485	40.0	38.0	41.0	34.0	41.0
18	38.501091086722376	40.0	38.0	41.0	34.0	41.0
19	38.51711704858039	40.0	38.0	41.0	34.0	41.0
20	38.417463793938765	40.0	37.0	41.0	34.0	41.0
21	38.40089729370453	40.0	37.0	41.0	34.0	41.0
22	38.4017461391546	40.0	37.0	41.0	34.0	41.0
23	38.416739072115824	40.0	37.0	41.0	34.0	41.0
24	38.335580237916965	40.0	37.0	41.0	34.0	41.0
25	38.268042970798916	40.0	37.0	41.0	34.0	41.0
26	38.143174601903496	40.0	37.0	41.0	34.0	41.0
27	38.096359974534636	40.0	37.0	41.0	34.0	41.0
28	38.13279025909806	40.0	37.0	41.0	34.0	41.0
29	38.160037397247656	40.0	37.0	41.0	34.0	41.0
30	38.033639504946926	40.0	37.0	41.0	34.0	41.0
31	38.06914086430084	40.0	37.0	41.0	34.0	41.0
32	38.03379966446581	40.0	37.0	41.0	34.0	41.0
33	38.018162089441084	40.0	37.0	41.0	34.0	41.0
34	37.98283490356395	40.0	37.0	41.0	34.0	41.0
35	37.98202809998759	40.0	37.0	41.0	34.0	41.0
36	37.912124475978075	40.0	37.0	41.0	34.0	41.0
37	37.876032528398284	40.0	37.0	41.0	33.0	41.0
38	37.82528398284691	40.0	36.0	41.0	33.0	41.0
39	37.78036924777078	40.0	36.0	41.0	33.0	41.0
40	37.68236763816761	40.0	36.0	41.0	33.0	41.0
41	37.63575521219134	40.0	36.0	41.0	33.0	41.0
42	37.64883023491397	40.0	36.0	41.0	33.0	41.0
43	37.58007375345844	40.0	36.0	41.0	33.0	41.0
44	37.47995003023011	40.0	36.0	41.0	33.0	41.0
45	37.34632694163387	40.0	35.0	41.0	33.0	41.0
46	37.25777874763264	39.0	35.0	41.0	32.0	41.0
47	37.22073985689747	39.0	35.0	41.0	32.0	41.0
48	37.19708629795276	39.0	35.0	41.0	32.0	41.0
49	37.184942202433625	39.0	35.0	41.0	32.0	41.0
50	37.053731516590524	39.0	35.0	41.0	32.0	41.0
51	36.04657839207851	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	11.0
10	9.0
11	5.0
12	13.0
13	10.0
14	5.0
15	10.0
16	17.0
17	18.0
18	59.0
19	115.0
20	211.0
21	330.0
22	537.0
23	719.0
24	1110.0
25	1621.0
26	2233.0
27	2898.0
28	3581.0
29	4662.0
30	5912.0
31	7508.0
32	10388.0
33	14806.0
34	27722.0
35	38484.0
36	32688.0
37	50636.0
38	100707.0
39	192296.0
40	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.231450524722625	20.945862078630313	23.545451269464387	10.277236127182674
2	32.496766779712594	23.806311085841497	24.732833902566956	18.96408823187895
3	32.51518512438389	24.641342777406297	25.563861606159733	17.279610492050082
4	29.00789186029285	26.691785017877805	24.58668834158822	19.713634780241122
5	25.511809762523473	30.21249164167511	23.965069208932096	20.310629386869323
6	26.984476538632478	34.82148219626748	23.481787860709264	14.712253404390774
7	83.76022518428354	4.6278092980608685	7.197969177300591	4.413996340354993
8	83.77283774639541	3.7076928620906426	7.351121717230362	5.168347674283586
9	77.50179178461748	6.571144860280839	9.640602039631473	6.286461315470208
10	37.7682171442757	31.570444162385737	16.772305215995132	13.889033477343435
11	27.071563277023913	24.003907892260692	28.59027591481115	20.33425291590424
12	25.106606179755037	23.218725850947543	30.947623833338007	20.727044135959417
13	22.92983811876629	25.952048240047088	32.2795504322305	18.83856320895612
14	20.31042918747072	28.23552258049017	29.714996136151605	21.739052095887505
15	19.159482844913533	26.28057545315134	34.21247562572322	20.347466076211905
16	21.817129861341897	26.73542848677283	30.823300006806782	20.6241416450785
17	21.54906286661515	26.131226701794986	28.937221472586693	23.38248895900317
18	22.69900821217933	25.037337187839086	31.25873369876397	21.004920901217613
19	21.759472434544808	27.339630271750664	28.537223074181885	22.363674219522643
20	23.362869417940267	27.97786595449067	29.065549287089944	19.59371534047912
21	22.25876973465572	27.732421491805837	30.65853590175815	19.350272871780295
22	21.67478808893658	25.002902891279717	28.86034490352391	24.461964116259793
23	19.847768377303794	27.422112423974276	29.501183178445732	23.228936020276194
24	21.206321496210226	25.02772761670624	31.123398905309692	22.64255198177385
25	19.167490820857573	28.238925970266386	28.857542111943495	23.736041096932546
26	20.62654403786171	28.026114009553517	28.710595753370356	22.636746199214418
27	21.24495998014022	27.920408727092184	29.09838198846051	21.73624930430709
28	19.153476862955504	27.50379377860349	29.6138954398581	23.728833918582907
29	21.226341436070324	25.24414316659393	29.482164235578633	24.04735116175711
30	21.704817998726732	28.527012904853233	28.471757870839355	21.296411225580677
31	23.33944608830395	28.57806375149649	25.816713446592804	22.26577671360675
32	22.275986882935403	28.0437315566304	26.865558095863477	22.81472346457071
33	21.124039543385212	28.131819292014846	26.241936969221346	24.502204195378596
34	20.14926867159691	27.18507633603069	28.880565042782614	23.785089949589793
35	19.93225252351342	26.633927391682118	28.917401732125196	24.516418352679267
36	22.24956056232007	28.966050185985242	26.27837325976673	22.50601599192796
37	20.79751432426697	28.112800349147747	28.333820485203265	22.755864841382017
38	19.40933169436759	28.523609515077013	27.060752509499466	25.006306281055934
39	20.8825990686724	27.423113420967283	27.240531569443167	24.453755940917155
40	21.921834146810223	26.858951515709645	28.909393756181156	22.309820581298972
41	19.490212251402397	26.571665378717203	28.316403137524976	25.621719232355428
42	22.003715700838033	24.575477175266567	28.623909413776122	24.79689771011928
43	22.595905521899812	24.213116263798742	28.141629062546297	25.049349151755145
44	21.25156656029405	25.693590816453188	26.710603761346302	26.34423886190646
45	21.63655000380379	25.24854755336315	26.901193588814458	26.213708854018602
46	22.49180183462729	26.355450028228116	27.876565058798562	23.276183078346033
47	18.99972372482993	26.0337295946763	30.542019851772366	24.424526828721408
48	19.784305167947274	24.93203230417496	29.839520162081435	25.444142365796335
49	21.98109316879612	23.300807604373958	30.58065833570236	24.13744089112756
50	20.670988304351134	24.33343610235795	29.25233532598468	25.743240267306238
51	19.692213444590813	23.87858306873646	27.332823492198226	29.096379994474496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	110.0
1	307.0
2	504.0
3	781.0
4	1058.0
5	722.5
6	387.0
7	408.0
8	429.0
9	469.5
10	510.0
11	515.0
12	520.0
13	578.5
14	637.0
15	617.5
16	598.0
17	625.0
18	652.0
19	790.0
20	928.0
21	1048.0
22	1168.0
23	1595.5
24	2023.0
25	2306.5
26	3462.0
27	4334.0
28	5114.0
29	5894.0
30	6591.5
31	7289.0
32	8668.0
33	10047.0
34	12179.5
35	14312.0
36	14900.0
37	15488.0
38	17555.0
39	19622.0
40	21539.0
41	23456.0
42	26855.0
43	30254.0
44	33125.5
45	35997.0
46	42154.0
47	48311.0
48	53875.5
49	59440.0
50	59647.5
51	59855.0
52	52007.0
53	44159.0
54	37563.0
55	30967.0
56	27064.5
57	23162.0
58	20104.5
59	17047.0
60	14763.5
61	12480.0
62	10791.0
63	9102.0
64	7671.0
65	6240.0
66	5316.0
67	4392.0
68	3344.0
69	2296.0
70	1715.0
71	1134.0
72	928.5
73	723.0
74	668.5
75	534.0
76	454.0
77	298.5
78	143.0
79	120.0
80	97.0
81	61.5
82	26.0
83	31.0
84	36.0
85	21.5
86	7.0
87	7.5
88	8.0
89	4.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	499502.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.594816287822034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.62981990977664	24.088308279328558
2	11.935142260468425	8.257881077398523
3	4.401825900028739	4.568410750274134
4	2.168519278883166	3.000781042782538
5	1.454803865088933	2.516433622378253
6	1.0479920752001082	2.1753055987584666
7	0.8813419069822639	2.1342902951167106
8	0.7501688733039062	2.0761563485432863
9	0.7232034505071996	2.2517181458114006
>10	6.896532045473876	43.25342667179336
>50	0.06557062772545942	1.5649960997718513
>100	0.04156709436167513	2.6382346571280095
>500	0.0017563560997890903	0.37064098131001516
>1k	0.0017563560997890903	1.1034164296048974
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	2158	0.43203030218097227	RNA PCR Primer, Index 48 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	1878	0.3759744705726904	RNA PCR Primer, Index 4 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT	1412	0.2826815508246213	TruSeq Adapter, Index 4 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	741	0.1483477543633459	RNA PCR Primer, Index 48 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	559	0.11191146381796269	No Hit
ACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	530	0.10610568125853351	TruSeq Adapter, Index 4 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.003987972020132E-4	0.0	0.0	0.29649530932809076	0.0
2	4.003987972020132E-4	0.0	0.0	1.4122065577315006	0.0
3	4.003987972020132E-4	0.0	0.0	1.9443365592129762	0.0
4	4.003987972020132E-4	0.0	0.0	2.731320395113533	0.0
5	4.003987972020132E-4	0.0	0.0	4.730911988340387	0.0
6	4.003987972020132E-4	0.0	0.0	5.6912685034294155	0.0
7	4.003987972020132E-4	0.0	0.0	6.627601090686324	0.0
8	4.003987972020132E-4	0.0	0.0	8.207174345648266	0.0
9	4.003987972020132E-4	0.0	0.0	8.716882014486428	0.0
10	4.003987972020132E-4	0.0	0.0	10.035995851868462	0.0
11	4.003987972020132E-4	0.0	0.0	12.063415161500854	0.0
12	4.003987972020132E-4	0.0	0.0	13.613158706071246	0.0
13	4.003987972020132E-4	0.0	0.0	14.216159294657478	0.0
14	4.003987972020132E-4	0.0	0.0	14.438580826503197	0.0
15	4.003987972020132E-4	0.0	0.0	14.929469751872865	0.0
16	4.003987972020132E-4	0.0	0.0	15.920256575549248	0.0
17	4.003987972020132E-4	0.0	0.0	17.21714827968657	0.0
18	4.003987972020132E-4	0.0	0.0	18.40913549895696	0.0
19	4.003987972020132E-4	0.0	0.0	19.24256559533295	0.0
20	4.003987972020132E-4	0.0	0.0	20.007127098590196	0.0
21	4.003987972020132E-4	0.0	0.0	20.963880024504405	0.0
22	4.003987972020132E-4	0.0	0.0	21.974086189845085	0.0
23	4.003987972020132E-4	0.0	0.0	23.04034818679405	0.0
24	4.003987972020132E-4	0.0	0.0	23.816120856372947	0.0
25	4.003987972020132E-4	0.0	0.0	24.506007983952017	0.0
26	4.003987972020132E-4	0.0	0.0	25.129629110594152	0.0
27	4.003987972020132E-4	0.0	0.0	25.75985681739012	0.0
28	4.003987972020132E-4	0.0	0.0	26.418713038186034	0.0
29	4.003987972020132E-4	0.0	0.0	27.07837005657635	0.0
30	4.003987972020132E-4	0.0	0.0	27.86555409187551	0.0
31	4.003987972020132E-4	0.0	0.0	28.52280871748261	0.0
32	4.003987972020132E-4	0.0	0.0	29.169853173761066	0.0
33	4.003987972020132E-4	0.0	0.0	29.819700421619935	0.0
34	4.003987972020132E-4	0.0	0.0	30.46514328270958	0.0
35	4.003987972020132E-4	0.0	0.0	31.151026422316626	0.0
36	4.003987972020132E-4	0.0	0.0	31.723396502916906	0.0
37	4.003987972020132E-4	0.0	0.0	32.3382088560206	0.0
38	6.005981958030198E-4	0.0	0.0	32.97043855680258	0.0
39	6.005981958030198E-4	0.0	0.0	33.603469055178955	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAG	35	1.2100827E-7	45.000004	1
GTTAGCG	35	1.2100827E-7	45.000004	1
ATTAGCG	20	7.029452E-4	45.0	1
TTAGCCG	25	3.887628E-5	45.0	1
CGACAGC	20	7.029452E-4	45.0	13
CGTTAGG	40	6.8012014E-9	45.0	2
CGCTAAG	20	7.029452E-4	45.0	19
TTGCGCG	20	7.029452E-4	45.0	1
ATAACGG	25	3.887628E-5	45.0	2
ATCGCTA	20	7.029452E-4	45.0	17
TAGCACG	30	2.1630276E-6	44.999996	1
TCGCGCG	30	2.1630276E-6	44.999996	1
CGAATGG	65	0.0	41.538464	2
CGCAAGG	65	0.0	41.538464	2
GCTACGA	60	3.6379788E-12	41.249996	10
ACGGGCG	100	0.0	40.5	5
TAACGGG	95	0.0	40.263157	3
TATGCGG	90	0.0	40.000004	2
GCGCAAG	45	1.9244908E-8	40.000004	1
CTAACGG	45	1.9244908E-8	40.000004	2
>>END_MODULE