FastQCFastQC Report
Sat 14 Jan 2017
SRR2934547.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934547.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198855
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC19570.9841341681124438RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC14980.7533127152950643RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT10180.511930803852053TruSeq Adapter, Index 10 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC6140.3087676950541852RNA PCR Primer, Index 35 (95% over 23bp)
GGGTTAGGGTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGT4750.23886751653214655No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.2192552362274019No Hit
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC3610.18153931256443137TruSeq Adapter, Index 10 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG3480.17500188579618314TruSeq Adapter, Index 10 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG3410.17148173292097257RNA PCR Primer, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG3190.1604183953131679RNA PCR Primer, Index 35 (95% over 22bp)
GGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT2970.1493550577053632TruSeq Adapter, Index 10 (95% over 22bp)
GGCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT2700.1357773251866938TruSeq Adapter, Index 10 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG2590.13024565638279148TruSeq Adapter, Index 10 (95% over 23bp)
GGGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC2290.11515928691760327RNA PCR Primer, Index 10 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG2090.10510170727414449TruSeq Adapter, Index 10 (95% over 23bp)
GGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC2080.10459882829197154TruSeq Adapter, Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCA207.0192065E-445.024
TATCAGA207.0192065E-445.022
CGGGTAT253.8791386E-545.06
CCTTATC207.0192065E-445.043
CAAACTT253.8791386E-545.030
TCGTTAG253.8791386E-545.01
GCCTTCT253.8791386E-545.040
GTACCCC207.0192065E-445.09
CGAACGG453.8198777E-1045.02
TTGTGCG351.2051532E-745.01
CAAGTAT207.0192065E-445.042
GGAGATA351.2051532E-745.08
CGAAAGG207.0192065E-445.02
CACGGAG207.0192065E-445.011
CATGCGG453.8198777E-1045.01
CGTGCGC207.0192065E-445.019
TTAGCCC207.0192065E-445.024
GCGCGAC207.0192065E-445.09
TTTCGTC253.8791386E-545.016
GACGTTA207.0192065E-445.031
Produced by FastQC (version 0.11.5)