##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934547.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	198855
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.944804002916698	33.0	31.0	34.0	30.0	34.0
2	32.083080636644794	33.0	31.0	34.0	30.0	34.0
3	32.13109049307284	34.0	31.0	34.0	30.0	34.0
4	35.71199617811973	37.0	35.0	37.0	33.0	37.0
5	35.780593900077946	37.0	35.0	37.0	35.0	37.0
6	35.80906690804858	37.0	35.0	37.0	35.0	37.0
7	36.035287018179076	37.0	35.0	37.0	35.0	37.0
8	35.70603706218099	37.0	35.0	37.0	35.0	37.0
9	37.385441653466096	39.0	37.0	39.0	34.0	39.0
10	37.04147745844963	39.0	37.0	39.0	33.0	39.0
11	37.036277689773954	39.0	37.0	39.0	33.0	39.0
12	36.9103215911091	39.0	35.0	39.0	33.0	39.0
13	36.87338010107867	39.0	35.0	39.0	33.0	39.0
14	37.925900781976814	40.0	37.0	41.0	33.0	41.0
15	38.11683890271806	40.0	37.0	41.0	33.0	41.0
16	38.193915164315705	40.0	37.0	41.0	34.0	41.0
17	38.194714741897364	40.0	37.0	41.0	34.0	41.0
18	38.212496542707	40.0	37.0	41.0	34.0	41.0
19	38.1862311734681	40.0	37.0	41.0	34.0	41.0
20	38.070609237886906	40.0	36.0	41.0	34.0	41.0
21	38.05347615096427	40.0	36.0	41.0	34.0	41.0
22	38.016157501697215	40.0	36.0	41.0	34.0	41.0
23	38.002479193382115	40.0	36.0	41.0	34.0	41.0
24	37.898820748786804	40.0	36.0	41.0	34.0	41.0
25	37.76501470921023	40.0	35.0	41.0	33.0	41.0
26	37.55274446204521	39.0	35.0	41.0	33.0	41.0
27	37.54300872495034	39.0	35.0	41.0	33.0	41.0
28	37.50107364662694	39.0	35.0	41.0	33.0	41.0
29	37.52812350707802	39.0	35.0	41.0	33.0	41.0
30	37.453048703829424	39.0	35.0	41.0	33.0	41.0
31	37.408206984989064	39.0	35.0	41.0	33.0	41.0
32	37.385562344421814	39.0	35.0	41.0	33.0	41.0
33	37.36534660933846	39.0	35.0	41.0	33.0	41.0
34	37.28851675844208	39.0	35.0	41.0	33.0	41.0
35	37.309300746775286	40.0	35.0	41.0	33.0	41.0
36	37.24644590279349	39.0	35.0	41.0	33.0	41.0
37	37.16425536194715	39.0	35.0	41.0	33.0	41.0
38	37.07987226873853	39.0	35.0	41.0	32.0	41.0
39	37.02140252948128	39.0	35.0	41.0	32.0	41.0
40	36.94698649769933	39.0	35.0	41.0	32.0	41.0
41	36.846103945085616	39.0	35.0	41.0	32.0	41.0
42	36.86046114002665	39.0	35.0	41.0	32.0	41.0
43	36.78772975283498	39.0	35.0	41.0	31.0	41.0
44	36.70541349224309	39.0	35.0	41.0	31.0	41.0
45	36.62649166478087	39.0	35.0	41.0	31.0	41.0
46	36.56534158054864	39.0	35.0	41.0	31.0	41.0
47	36.52212918961052	39.0	35.0	41.0	31.0	41.0
48	36.54144477131578	39.0	35.0	41.0	31.0	41.0
49	36.52417590706796	39.0	35.0	41.0	31.0	41.0
50	36.38167508988962	39.0	35.0	40.0	31.0	41.0
51	35.32518166503231	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	4.0
11	2.0
12	6.0
13	2.0
14	4.0
15	3.0
16	8.0
17	36.0
18	37.0
19	78.0
20	111.0
21	209.0
22	319.0
23	450.0
24	693.0
25	1009.0
26	1300.0
27	1633.0
28	1965.0
29	2229.0
30	2932.0
31	3853.0
32	5006.0
33	7345.0
34	14160.0
35	17498.0
36	14307.0
37	20886.0
38	39235.0
39	63493.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.36488898946468	18.306303588041537	22.18450629855925	11.144301123934525
2	36.4969450101833	20.85690578562269	21.927032259686708	20.719116944507306
3	33.56164039123985	22.816122300168466	24.86786854743406	18.75436876115763
4	30.6504739634407	25.69711598903724	23.99034472354228	19.662065323979785
5	25.36370722385658	27.62515400668829	24.822106560056323	22.189032209398807
6	28.975886952804807	32.86967891176988	23.410022378114707	14.744411757310601
7	78.95300595911594	4.455004903070076	10.78926856252043	5.802720575293556
8	74.18621608709864	4.952352216439114	12.263206859269317	8.598224837192928
9	67.8871539564004	6.858263558874557	16.074023786175857	9.180558698549195
10	35.98199693243821	25.764501772648412	22.47165019738	15.781851097533378
11	29.368132558899703	24.35644062256418	26.67923864122099	19.59618817731513
12	27.970128988458924	21.107842397726987	30.683663976264114	20.238364637549974
13	24.159312061552388	26.969399813934775	30.76865052425134	18.1026376002615
14	19.549420431973047	27.206758693520406	29.967564305649848	23.276256568856706
15	18.332956174096704	25.478866510774186	35.84772824419804	20.34044907093108
16	20.01005757964346	24.970958738779515	31.76233939302507	23.256644288551957
17	19.02340901662015	26.02398732744965	27.81172210907445	27.14088154685575
18	20.322848306555027	23.71024113047195	33.168891906162784	22.79801865681024
19	19.371904151265998	27.506474566895477	28.073219179804383	25.048402102034146
20	23.66749641698725	27.268109929345503	29.3812074124362	19.68318624123105
21	21.28083276759448	27.253526438862487	31.00299212994393	20.462648663599108
22	19.57909029192125	25.92944607880114	28.420205677503706	26.07125795177391
23	19.475497221593624	27.039299992456815	28.84513841743984	24.64006436850972
24	22.71604938271605	22.799024414774586	31.882527469764398	22.602398732744966
25	19.426717960322847	25.631238842372582	28.378969600965526	26.56307359633904
26	18.323904352417593	26.739081240099573	28.79837067209776	26.13864373538508
27	20.592894319981898	24.92569963038395	29.64169872520178	24.839707324432375
28	16.56030776193709	25.99683186241231	29.338462698951496	28.1043976766991
29	18.284176912825927	22.277036031279074	31.161901888310577	28.27688516758442
30	20.570767644766285	23.09823740916748	31.545095672726358	24.78589927333987
31	18.121244122601894	25.012697694299867	27.88564531945387	28.98041286364436
32	18.439063639335192	23.76807221342184	30.935606346332754	26.85725780091021
33	18.661336149455636	22.46310125468306	29.962032636845944	28.91352995901536
34	16.27618113700938	20.952452792235547	31.67735284503784	31.094013225717234
35	16.510019863719798	20.993185989791556	33.09346005883684	29.403334087651807
36	18.74431118151417	21.25719745543235	31.66528374946569	28.333207613587792
37	18.68597721958211	22.48019914007694	32.19632395464031	26.63749968570064
38	17.7234668477031	24.4675768776244	31.41384425837922	26.395112016293282
39	19.91601920997712	21.260214729325387	32.182746222121644	26.641019838575847
40	21.955696361670565	19.44783887757411	31.930803852053003	26.66566090870232
41	16.417490130999976	21.82142767343039	30.078197681727893	31.682884513841742
42	18.852933041663526	20.117170802846296	30.668577606798923	30.36131854869126
43	20.71157375977471	19.226572125418016	31.296673455532925	28.765180659274346
44	18.124261396494934	21.04347388800885	30.865203288828543	29.967061426667673
45	19.740514445198762	18.733750722888537	30.387971134746422	31.137763697166278
46	20.20517462472656	20.492318523547308	31.817153202081922	27.48535364964421
47	16.1308491111614	20.16896733801011	35.14420054813809	28.555983002690404
48	18.081516683010236	17.67217319152146	34.32149053330316	29.924819592165147
49	19.96982726106962	17.91657237685751	35.14118327424505	26.972417087827814
50	19.813934776596014	18.140353523924468	34.11279575570139	27.93291594377813
51	17.91958965075055	18.198687485856528	30.501118905735336	33.38060395765759
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	190.0
1	306.5
2	423.0
3	644.5
4	866.0
5	617.0
6	368.0
7	340.5
8	313.0
9	328.0
10	343.0
11	372.5
12	402.0
13	455.5
14	509.0
15	502.5
16	496.0
17	543.0
18	590.0
19	618.0
20	646.0
21	579.0
22	512.0
23	510.5
24	509.0
25	648.5
26	1035.5
27	1283.0
28	1261.5
29	1240.0
30	1501.5
31	1763.0
32	2087.5
33	2412.0
34	2957.0
35	3502.0
36	4044.0
37	4586.0
38	4887.5
39	5189.0
40	6116.0
41	7043.0
42	8009.0
43	8975.0
44	11310.0
45	13645.0
46	16187.0
47	18729.0
48	22340.0
49	25951.0
50	27209.5
51	28468.0
52	23125.5
53	17783.0
54	15459.5
55	13136.0
56	11082.5
57	9029.0
58	8185.0
59	7341.0
60	6930.0
61	6519.0
62	5687.0
63	4855.0
64	3959.5
65	3064.0
66	2811.0
67	2558.0
68	1981.5
69	1405.0
70	1237.5
71	1070.0
72	1003.0
73	936.0
74	773.0
75	506.0
76	402.0
77	289.0
78	176.0
79	129.5
80	83.0
81	86.5
82	90.0
83	61.5
84	33.0
85	26.5
86	20.0
87	12.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	198855.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.83498026199995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.41976291849174	22.465615649593925
2	13.065519036971237	8.580121193834705
3	5.299108647042607	5.219883834955118
4	2.914509755873434	3.8279148123004196
5	1.7045976659417403	2.7985215357924114
6	1.082794743774313	2.133212642377612
7	0.7979293656384966	1.8339996479847125
8	0.5804514963090024	1.5247290739483543
9	0.4548656844426746	1.3441955193482689
>10	5.418568321744724	39.66608835583717
>50	0.1899102520905443	4.0783485454225445
>100	0.0658559745152694	3.969223806291016
>500	0.0015315342910527767	0.3087676950541852
>1k	0.00459460287315833	2.249377687259561
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1957	0.9841341681124438	RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1498	0.7533127152950643	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	1018	0.511930803852053	TruSeq Adapter, Index 10 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	614	0.3087676950541852	RNA PCR Primer, Index 35 (95% over 23bp)
GGGTTAGGGTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGT	475	0.23886751653214655	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	436	0.2192552362274019	No Hit
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	361	0.18153931256443137	TruSeq Adapter, Index 10 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	348	0.17500188579618314	TruSeq Adapter, Index 10 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	341	0.17148173292097257	RNA PCR Primer, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	319	0.1604183953131679	RNA PCR Primer, Index 35 (95% over 22bp)
GGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	297	0.1493550577053632	TruSeq Adapter, Index 10 (95% over 22bp)
GGCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	270	0.1357773251866938	TruSeq Adapter, Index 10 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	259	0.13024565638279148	TruSeq Adapter, Index 10 (95% over 23bp)
GGGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	229	0.11515928691760327	RNA PCR Primer, Index 10 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	209	0.10510170727414449	TruSeq Adapter, Index 10 (95% over 23bp)
GGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	208	0.10459882829197154	TruSeq Adapter, Index 10 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.028789821729401E-4	0.0	0.0	0.5290286892459329	0.0
2	5.028789821729401E-4	0.0	0.0	2.870433230243142	0.0
3	5.028789821729401E-4	0.0	0.0	3.9501144049684442	0.0
4	5.028789821729401E-4	0.0	0.0	5.241507631188554	0.0
5	5.028789821729401E-4	0.0	0.0	8.412662492771114	0.0
6	5.028789821729401E-4	0.0	0.0	9.924316713182973	0.0
7	5.028789821729401E-4	0.0	0.0	11.330869226320686	0.0
8	5.028789821729401E-4	0.0	0.0	13.58779009831284	0.0
9	5.028789821729401E-4	0.0	0.0	14.367252520680898	0.0
10	5.028789821729401E-4	0.0	0.0	16.19270322596867	0.0
11	5.028789821729401E-4	0.0	0.0	18.986698850921524	0.0
12	5.028789821729401E-4	0.0	0.0	20.822207135852757	0.0
13	5.028789821729401E-4	0.0	0.0	21.615247290739482	0.0
14	5.028789821729401E-4	0.0	0.0	21.99089789042267	0.0
15	5.028789821729401E-4	0.0	0.0	22.728621357270374	0.0
16	5.028789821729401E-4	0.0	0.0	24.249830278343516	0.0
17	5.028789821729401E-4	0.0	0.0	25.86859772195821	0.0
18	0.0010057579643458802	0.0	0.0	27.472278796107716	0.0
19	0.0010057579643458802	0.0	0.0	28.417691282592845	0.0
20	0.0010057579643458802	0.0	0.0	29.303764049181563	0.0
21	0.0010057579643458802	0.0	0.0	30.466420255965403	0.0
22	0.0010057579643458802	0.0	0.0	31.664277991501343	0.0
23	0.0010057579643458802	0.0	0.0	32.75150235095924	0.0
24	0.0010057579643458802	0.0	0.0	33.64109527042317	0.0
25	0.0010057579643458802	0.0	0.0	34.46883407507983	0.0
26	0.0010057579643458802	0.0	0.0	35.217118000553164	0.0
27	0.0010057579643458802	0.0	0.0	35.963893289079984	0.0
28	0.0010057579643458802	0.0	0.0	36.625682029619576	0.0
29	0.0010057579643458802	0.0	0.0	37.33373563651907	0.0
30	0.0015086369465188202	0.0	0.0	38.15745140931835	0.0
31	0.0015086369465188202	0.0	0.0	38.85846471046742	0.0
32	0.0015086369465188202	0.0	0.0	39.60171984611903	0.0
33	0.0015086369465188202	0.0	0.0	40.29418420457117	0.0
34	0.0015086369465188202	0.0	0.0	41.00575796434588	0.0
35	0.0015086369465188202	0.0	0.0	41.678610042493276	0.0
36	0.0015086369465188202	0.0	0.0	42.34643333081894	0.0
37	0.0015086369465188202	0.0	0.0	43.04141208418194	0.0
38	0.0015086369465188202	0.0	0.0	43.64838701566468	0.0
39	0.0015086369465188202	0.0	0.0	44.30665560332906	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCA	20	7.0192065E-4	45.0	24
TATCAGA	20	7.0192065E-4	45.0	22
CGGGTAT	25	3.8791386E-5	45.0	6
CCTTATC	20	7.0192065E-4	45.0	43
CAAACTT	25	3.8791386E-5	45.0	30
TCGTTAG	25	3.8791386E-5	45.0	1
GCCTTCT	25	3.8791386E-5	45.0	40
GTACCCC	20	7.0192065E-4	45.0	9
CGAACGG	45	3.8198777E-10	45.0	2
TTGTGCG	35	1.2051532E-7	45.0	1
CAAGTAT	20	7.0192065E-4	45.0	42
GGAGATA	35	1.2051532E-7	45.0	8
CGAAAGG	20	7.0192065E-4	45.0	2
CACGGAG	20	7.0192065E-4	45.0	11
CATGCGG	45	3.8198777E-10	45.0	1
CGTGCGC	20	7.0192065E-4	45.0	19
TTAGCCC	20	7.0192065E-4	45.0	24
GCGCGAC	20	7.0192065E-4	45.0	9
TTTCGTC	25	3.8791386E-5	45.0	16
GACGTTA	20	7.0192065E-4	45.0	31
>>END_MODULE