##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934546.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	573036
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20924165322946	33.0	31.0	34.0	30.0	34.0
2	32.32454505476096	34.0	31.0	34.0	30.0	34.0
3	32.38564069273135	34.0	31.0	34.0	30.0	34.0
4	35.91891085376835	37.0	35.0	37.0	35.0	37.0
5	35.95069768740533	37.0	35.0	37.0	35.0	37.0
6	35.96075290208643	37.0	35.0	37.0	35.0	37.0
7	36.127060079994976	37.0	35.0	37.0	35.0	37.0
8	35.90042161399982	37.0	35.0	37.0	35.0	37.0
9	37.60527785339839	39.0	38.0	39.0	35.0	39.0
10	37.30775029841057	39.0	37.0	39.0	34.0	39.0
11	37.22601546848715	39.0	37.0	39.0	34.0	39.0
12	37.043588884467994	39.0	37.0	39.0	34.0	39.0
13	36.97164750556684	39.0	37.0	39.0	33.0	39.0
14	38.175622473980695	40.0	37.0	41.0	33.0	41.0
15	38.34113912563958	40.0	37.0	41.0	34.0	41.0
16	38.42088804193803	40.0	37.0	41.0	34.0	41.0
17	38.396678393678584	40.0	37.0	41.0	34.0	41.0
18	38.39626480709764	40.0	37.0	41.0	34.0	41.0
19	38.384211463154145	40.0	37.0	41.0	34.0	41.0
20	38.26316147676586	40.0	37.0	41.0	34.0	41.0
21	38.24470888390956	40.0	37.0	41.0	34.0	41.0
22	38.22216579761132	40.0	37.0	41.0	34.0	41.0
23	38.2511325640972	40.0	37.0	41.0	34.0	41.0
24	38.1538193062914	40.0	36.0	41.0	34.0	41.0
25	38.07787468850125	40.0	36.0	41.0	34.0	41.0
26	37.900456515821	40.0	36.0	41.0	34.0	41.0
27	37.87753648985404	40.0	36.0	41.0	34.0	41.0
28	37.90733566477499	40.0	36.0	41.0	34.0	41.0
29	37.91917087233612	40.0	36.0	41.0	34.0	41.0
30	37.80330380639262	40.0	36.0	41.0	34.0	41.0
31	37.8102300728052	40.0	36.0	41.0	34.0	41.0
32	37.76250357743667	40.0	35.0	41.0	34.0	41.0
33	37.75266998932004	40.0	35.0	41.0	33.0	41.0
34	37.70539023726258	40.0	35.0	41.0	33.0	41.0
35	37.69543623786289	40.0	35.0	41.0	33.0	41.0
36	37.60965279668293	40.0	35.0	41.0	33.0	41.0
37	37.574805073328726	40.0	35.0	41.0	33.0	41.0
38	37.51142860134442	40.0	35.0	41.0	33.0	41.0
39	37.44246783797178	40.0	35.0	41.0	33.0	41.0
40	37.358111881277964	39.0	35.0	41.0	33.0	41.0
41	37.27868057155222	39.0	35.0	41.0	33.0	41.0
42	37.26048450708158	39.0	35.0	41.0	33.0	41.0
43	37.20103274488863	39.0	35.0	41.0	33.0	41.0
44	37.10900362280904	39.0	35.0	41.0	33.0	41.0
45	36.99843639841127	39.0	35.0	41.0	33.0	41.0
46	36.921306863792154	39.0	35.0	41.0	32.0	41.0
47	36.88266531247601	39.0	35.0	41.0	32.0	41.0
48	36.846920612317554	39.0	35.0	41.0	32.0	41.0
49	36.83882338980448	39.0	35.0	41.0	32.0	41.0
50	36.716963681164884	39.0	35.0	41.0	32.0	41.0
51	35.80042789632763	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	8.0
9	10.0
10	15.0
11	8.0
12	8.0
13	11.0
14	12.0
15	15.0
16	37.0
17	51.0
18	94.0
19	194.0
20	267.0
21	486.0
22	681.0
23	1069.0
24	1452.0
25	2084.0
26	2842.0
27	3702.0
28	4354.0
29	5362.0
30	6882.0
31	8889.0
32	12229.0
33	17847.0
34	37559.0
35	52350.0
36	40073.0
37	60112.0
38	107471.0
39	206664.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.06412162586644	20.170635003734493	23.295220544608018	10.47002282579105
2	33.92422116586044	23.524874527952868	24.0754507570205	18.475453549166197
3	34.79048436747429	23.693624833343804	24.239838334764308	17.276052464417592
4	29.743332007064126	26.037980161804843	24.14316029010394	20.075527541027093
5	26.790986953699242	29.96146838942056	22.77064617231727	20.476898484562923
6	27.792145694162322	34.98715612980685	22.69996998443379	14.520728191597037
7	82.98745628546898	5.723549654820989	7.248410222045385	4.0405838376646495
8	83.44240152451154	4.39064910407025	7.383305760894604	4.783643610523597
9	76.3709435358337	7.453458421460432	9.733768908061622	6.441829134644246
10	44.02498272359851	24.32168310542444	16.761076092950532	14.89225807802651
11	32.93370748085635	24.390963220460844	22.473631674100755	20.201697624582053
12	29.942097878667305	22.198954341437535	27.72600674303185	20.132941036863304
13	25.402767016382917	24.54784690665159	29.98624868245625	20.063137394509244
14	21.938935773668668	26.405496338798955	29.131852100042583	22.52371578748979
15	20.778450219532456	25.191436489156004	33.312741258838884	20.717372032472657
16	24.121346651868294	23.76395200301552	30.37488046126247	21.73982088385372
17	23.85469673807579	24.04421362706706	27.290781032954296	24.810308601902847
18	24.549766506816326	23.8299164450401	29.69516749383983	21.925149554303744
19	24.853935878374134	25.54237430109103	26.84613183115895	22.757557989375883
20	25.661215002198816	25.744455845706028	27.719898924325868	20.874430227769285
21	25.367516176994116	26.537948750165786	27.943096070752972	20.15143900208713
22	24.000062823278117	24.18731109389288	27.29357317864846	24.51905290418054
23	23.291381344278545	25.22389518285064	28.198926419980598	23.28579705289022
24	24.062537083185003	23.903559287723635	28.95751750326332	23.076386125828048
25	21.93928479188044	25.916696333214666	28.10556404833204	24.03845482657285
26	21.920786826656617	27.163738403869914	27.592158258817946	23.323316510655527
27	22.393706503605358	27.134246364975322	27.45865879281581	23.01338833860351
28	19.599117681960646	26.685583453744616	29.274077021338975	24.441221842955766
29	21.642619311875695	25.734334317564688	28.618271801422594	24.004774569137016
30	24.528999923215995	26.28473603752644	27.317131907942954	21.86913213131461
31	24.205111022693167	27.661089355642577	24.842418277385715	23.291381344278545
32	24.384855401754862	26.705826510027293	26.27217138190271	22.637146706315136
33	25.013262692047274	25.408525816877127	26.403925756845993	23.174285734229613
34	21.47892977055543	25.658248347398764	27.37088071255558	25.49194116949022
35	22.440998471300233	25.341165302005457	27.405608024626726	24.812228202067583
36	24.44506104328524	26.44877459705847	26.830949538946943	22.275214820709344
37	22.461416036688796	27.027446792173613	28.29700053748805	22.214136633649545
38	22.48096105654793	27.018197809561702	26.31091240340921	24.189928730481157
39	24.012103951584194	24.418884677402467	28.213585184874944	23.35542618613839
40	24.112272178362268	24.065503737985047	27.408923697638542	24.413300386014143
41	20.97302787259439	24.632134804794113	26.981201879114053	27.41363544349744
42	21.940855373833408	24.735269686372234	27.683077502984105	25.640797436810253
43	22.412727996146838	23.948408127936116	29.281580912892036	24.35728296302501
44	21.994080651128375	25.076260479271806	27.955660726376703	24.973998143223113
45	23.432035683621972	24.045086172596484	26.498160674023968	26.024717469757576
46	24.242630480458473	24.746438269148886	26.535680131789274	24.47525111860337
47	20.849126407415937	24.82095365736184	29.483313439295262	24.846606495926956
48	21.394990890624673	23.332740002373324	29.866884454030814	25.40538465297119
49	22.636448669891596	22.880586909024913	30.712904599361995	23.7700598217215
50	22.25741489190906	22.619870304832506	29.657124508756866	25.465590294501567
51	20.760650290732173	23.51213536322325	27.119936618292744	28.607277727751836
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	186.0
1	534.5
2	883.0
3	1179.0
4	1475.0
5	1046.0
6	617.0
7	615.5
8	614.0
9	674.5
10	735.0
11	759.5
12	784.0
13	825.5
14	867.0
15	802.0
16	737.0
17	766.5
18	796.0
19	968.5
20	1141.0
21	1045.0
22	949.0
23	1371.5
24	1794.0
25	1911.0
26	2782.0
27	3536.0
28	4385.5
29	5235.0
30	6068.0
31	6901.0
32	8155.0
33	9409.0
34	11234.0
35	13059.0
36	14376.5
37	15694.0
38	17068.5
39	18443.0
40	20800.0
41	23157.0
42	26584.5
43	30012.0
44	34340.0
45	38668.0
46	42495.5
47	46323.0
48	51185.5
49	56048.0
50	56906.5
51	57765.0
52	51262.5
53	44760.0
54	40343.0
55	35926.0
56	33874.5
57	31823.0
58	30416.5
59	29010.0
60	26656.0
61	24302.0
62	22495.5
63	20689.0
64	18335.0
65	15981.0
66	13982.0
67	11983.0
68	9829.0
69	7675.0
70	6260.5
71	4846.0
72	4323.0
73	3800.0
74	2995.5
75	1689.0
76	1187.0
77	806.5
78	426.0
79	397.0
80	368.0
81	265.0
82	162.0
83	95.0
84	28.0
85	23.0
86	18.0
87	10.5
88	3.0
89	1.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	573036.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.41265157097681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.42322434177476	19.724978841566024
2	12.037650704922473	6.840431504241713
3	4.321054142254237	3.6831781728958712
4	2.1379106576417803	2.4297484242181517
5	1.2622405282743527	1.7931800164312446
6	0.8971868679643462	1.5294874724116168
7	0.6965885308155731	1.3854349050081067
8	0.5213386146198165	1.1850089926150882
9	0.4855539001804962	1.2416286406281565
>10	7.969921230256722	53.62715421085825
>50	0.20072297853880416	3.4649252612651886
>100	0.04350033590624239	2.1131382759285455
>500	0.001242866740178354	0.2357152070826043
>1k	0.0018643001102675307	0.7459900748494405
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	1845	0.3219693003580927	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	1286	0.22441871016829656	No Hit
CTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGCT	1094	0.19091296183834872	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	743	0.12966026567266278	No Hit
TCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	592	0.1033093906840059	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.745091058851451E-4	0.0	0.0	0.1977188169678694	0.0
2	1.745091058851451E-4	0.0	0.0	0.9472354267445675	0.0
3	1.745091058851451E-4	0.0	0.0	1.2791517461381134	0.0
4	1.745091058851451E-4	0.0	0.0	1.7496282956044646	0.0
5	3.490182117702902E-4	0.0	0.0	3.1413384150384966	0.0
6	3.490182117702902E-4	0.0	0.0	3.8458316754968274	0.0
7	3.490182117702902E-4	0.0	0.0	4.500240822566122	0.0
8	3.490182117702902E-4	0.0	0.0	5.4986074173350366	0.0
9	3.490182117702902E-4	0.0	0.0	5.844658974305279	0.0
10	3.490182117702902E-4	0.0	0.0	6.7571670889787026	0.0
11	5.235273176554352E-4	0.0	0.0	8.256549326743869	0.0
12	5.235273176554352E-4	0.0	0.0	9.410229025750564	0.0
13	5.235273176554352E-4	0.0	0.0	9.856448809498879	0.0
14	5.235273176554352E-4	0.0	0.0	10.038461806937086	0.0
15	5.235273176554352E-4	0.0	0.0	10.439658241367034	0.0
16	5.235273176554352E-4	0.0	0.0	11.292135223615968	0.0
17	5.235273176554352E-4	0.0	0.0	12.246525523701827	0.0
18	5.235273176554352E-4	0.0	0.0	13.161127747645873	0.0
19	5.235273176554352E-4	0.0	0.0	13.746605797890535	0.0
20	6.980364235405804E-4	0.0	0.0	14.356864141170886	0.0
21	6.980364235405804E-4	0.0	0.0	15.132731625936241	0.0
22	6.980364235405804E-4	0.0	0.0	15.903712855736812	0.0
23	6.980364235405804E-4	0.0	0.0	16.67870779497274	0.0
24	6.980364235405804E-4	0.0	0.0	17.274132864252856	0.0
25	6.980364235405804E-4	0.0	0.0	17.861879532874024	0.0
26	6.980364235405804E-4	0.0	0.0	18.36237164855262	0.0
27	6.980364235405804E-4	0.0	0.0	18.89794009451413	0.0
28	6.980364235405804E-4	0.0	0.0	19.430541885675595	0.0
29	6.980364235405804E-4	0.0	0.0	20.008516044367195	0.0
30	6.980364235405804E-4	0.0	0.0	20.61301558715334	0.0
31	6.980364235405804E-4	0.0	0.0	21.191862291374363	0.0
32	6.980364235405804E-4	0.0	0.0	21.739297356536063	0.0
33	6.980364235405804E-4	0.0	0.0	22.293189258615513	0.0
34	6.980364235405804E-4	0.0	0.0	22.832771414012385	0.0
35	8.725455294257254E-4	0.0	0.0	23.411792627339295	0.0
36	8.725455294257254E-4	0.0	0.0	23.970047257065875	0.0
37	0.0010470546353108705	0.0	0.0	24.511898030839248	0.0
38	0.0010470546353108705	0.0	0.0	25.052527240871427	0.0
39	0.0010470546353108705	0.0	0.0	25.59699565123308	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTC	40	6.8048394E-9	45.000004	20
CCGATTG	20	7.0303207E-4	45.000004	11
ACGTTAG	25	3.8883478E-5	45.000004	1
CGTGCAA	20	7.0303207E-4	45.000004	15
TCTAGCG	35	1.210501E-7	45.000004	1
TATTGCG	20	7.0303207E-4	45.000004	1
TCTACGG	25	3.8883478E-5	45.000004	2
TCGAGAG	45	3.8380676E-10	45.000004	1
CGTCAAT	20	7.0303207E-4	45.000004	17
GCGTAAG	25	3.8883478E-5	45.000004	1
ATTTCGC	20	7.0303207E-4	45.000004	21
CGTTAGG	85	0.0	42.352943	2
GTATGCG	70	0.0	41.785717	1
CGTCGCG	60	3.6379788E-12	41.250004	16
TTAGGCG	45	1.9254003E-8	40.000004	1
TCGATCA	45	1.9254003E-8	40.000004	17
ACGGGCC	40	3.45417E-7	39.375004	5
CGGGGTA	80	0.0	39.375004	6
CGGTAGT	75	0.0	39.0	12
ACAACGA	35	6.2428044E-6	38.57143	13
>>END_MODULE