SRR2934542.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934542.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	922240
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	4419	0.4791594378903539	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	3213	0.34839087439278277	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2067	0.22412820957668286	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1719	0.1863939972241499	RNA PCR Primer, Index 40 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1195	0.12957581540596808	Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	1073	0.1163471547536433	Illumina PCR Primer Index 8 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGT	20	7.032561E-4	45.0	38
GTTTACG	45	3.8380676E-10	45.0	1
GCGTTAG	50	2.1827873E-11	45.0	1
TATTGCG	45	3.8380676E-10	45.0	1
CGGTCTA	20	7.032561E-4	45.0	31
ACGGGTA	80	0.0	42.1875	5
GTTAGCG	55	6.002665E-11	40.909092	1
TAGGGAT	700	0.0	40.500004	5
TAGCACG	50	1.0804797E-9	40.5	1
TTTACGG	90	0.0	40.0	2
TGCGTAG	40	3.4572258E-7	39.375	1
CACGACC	630	0.0	39.285713	27
AAACACG	610	0.0	39.09836	40
GCGCGAC	75	0.0	39.000004	9
CGACCAA	630	0.0	38.92857	29
ACACGAC	640	0.0	38.671875	26
CGACAAT	35	6.246946E-6	38.571426	20
CGTTTTT	1010	0.0	38.539604	1
AACACGT	615	0.0	38.414635	41
CGCACGG	130	0.0	38.07692	2