FastQCFastQC Report
Sat 14 Jan 2017
SRR2934541.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934541.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217593
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80970.665000537946588No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG26960.22142045823193793No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC18230.14972162290683338No Hit
CGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTG17020.13978398364642372RNA PCR Primer, Index 30 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC16900.13879843264539135RNA PCR Primer, Index 30 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTT13680.11235281411768956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAG502.1827873E-1145.01
CGTTTTT34700.041.109511
TATACGG1050.040.7142872
GCGTTAG501.0822987E-940.51
GTATACG501.0822987E-940.51
TTACGAG451.9284926E-840.0000041
TATCACG356.248594E-638.5714261
TACGTCG356.248594E-638.57142624
TACGTAG601.5643309E-1037.4999961
TACGGGA2950.037.3728834
GCGATGA2700.036.6666689
TTACACG9550.036.51832634
AGGGCGA9750.036.4615366
ACCGGTA751.8189894E-1236.041
TCGGAAT250.002107157436.019
CGTAGCA250.002107157436.014
GCGTAAC250.002107157436.032
CACAACG4600.035.7065212
ACACGCG9850.035.4060936
TACACGC9850.035.4060935
Produced by FastQC (version 0.11.5)