##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934541.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1217593 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25452511635662 33.0 31.0 34.0 30.0 34.0 2 32.37728863421521 34.0 31.0 34.0 30.0 34.0 3 32.410974767430496 34.0 31.0 34.0 30.0 34.0 4 35.955385748768265 37.0 35.0 37.0 35.0 37.0 5 35.96236016468557 37.0 35.0 37.0 35.0 37.0 6 35.9759402361873 37.0 35.0 37.0 35.0 37.0 7 36.15673381827918 37.0 36.0 37.0 35.0 37.0 8 35.931930456236195 37.0 36.0 37.0 35.0 37.0 9 37.64793325848621 39.0 38.0 39.0 35.0 39.0 10 37.281809274527696 39.0 37.0 39.0 34.0 39.0 11 37.197513454824396 39.0 37.0 39.0 34.0 39.0 12 37.057799281040545 39.0 37.0 39.0 34.0 39.0 13 37.04555955890022 39.0 37.0 39.0 33.0 39.0 14 38.17697128679288 40.0 37.0 41.0 33.0 41.0 15 38.33839222137446 40.0 38.0 41.0 34.0 41.0 16 38.43912046143498 40.0 38.0 41.0 34.0 41.0 17 38.42831964375616 40.0 37.0 41.0 34.0 41.0 18 38.38660701892997 40.0 37.0 41.0 34.0 41.0 19 38.34549640150691 40.0 37.0 41.0 34.0 41.0 20 38.202373863844485 40.0 37.0 41.0 34.0 41.0 21 38.16920596619725 40.0 36.0 41.0 34.0 41.0 22 38.150496923027646 40.0 36.0 41.0 34.0 41.0 23 38.15475778852211 40.0 36.0 41.0 34.0 41.0 24 38.075738773136834 40.0 36.0 41.0 34.0 41.0 25 37.98289740496209 40.0 36.0 41.0 34.0 41.0 26 37.85188975297986 40.0 36.0 41.0 34.0 41.0 27 37.81930333042322 40.0 36.0 41.0 34.0 41.0 28 37.80118397526924 40.0 35.0 41.0 33.0 41.0 29 37.788646123951104 40.0 35.0 41.0 33.0 41.0 30 37.664713085571286 40.0 35.0 41.0 33.0 41.0 31 37.641109139096564 40.0 35.0 41.0 33.0 41.0 32 37.542154069545404 40.0 35.0 41.0 33.0 41.0 33 37.47724403803241 40.0 35.0 41.0 33.0 41.0 34 37.34419958064805 40.0 35.0 41.0 33.0 41.0 35 37.28933724159058 40.0 35.0 41.0 33.0 41.0 36 37.197417363601794 40.0 35.0 41.0 33.0 41.0 37 37.15106032968323 40.0 35.0 41.0 32.0 41.0 38 37.06266954557065 39.0 35.0 41.0 32.0 41.0 39 37.00312501796577 39.0 35.0 41.0 32.0 41.0 40 36.91483689541579 39.0 35.0 41.0 32.0 41.0 41 36.80765740276102 39.0 35.0 41.0 32.0 41.0 42 36.7824305823046 39.0 35.0 41.0 32.0 41.0 43 36.72633137674083 39.0 35.0 41.0 32.0 41.0 44 36.63469402337234 39.0 35.0 41.0 31.0 41.0 45 36.4930810213265 39.0 35.0 41.0 31.0 41.0 46 36.429818502570235 39.0 35.0 41.0 31.0 41.0 47 36.3702173057828 38.0 35.0 41.0 31.0 41.0 48 36.30306596703496 38.0 35.0 41.0 31.0 41.0 49 36.25224356578922 38.0 35.0 41.0 31.0 41.0 50 36.14067262213236 38.0 35.0 40.0 31.0 41.0 51 35.18715449251104 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 16.0 9 23.0 10 25.0 11 24.0 12 19.0 13 26.0 14 39.0 15 52.0 16 88.0 17 138.0 18 253.0 19 451.0 20 739.0 21 1156.0 22 1740.0 23 2677.0 24 4095.0 25 6247.0 26 9047.0 27 11083.0 28 12589.0 29 14055.0 30 16505.0 31 20890.0 32 27233.0 33 40806.0 34 82351.0 35 92706.0 36 90204.0 37 134162.0 38 237302.0 39 410474.0 40 377.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.05314994419318 19.88948688108424 22.425227477490424 12.632135697232162 2 36.30178557202612 22.633425126458512 22.52780691084788 18.53698239066749 3 33.694674657295174 23.2567040053614 25.654713849373312 17.393907487970118 4 29.166806970802227 25.661202060130112 25.108554336301207 20.06343663276645 5 25.785710003260533 29.59092241824649 24.8117392264903 19.811628352002682 6 27.827689548149504 33.63915528423701 24.453245049864776 14.079910117748707 7 82.7335571081634 4.80948888503794 8.46161237786354 3.9953416289351202 8 82.92894259411807 4.087654906031819 8.808444200976846 4.1749582988732685 9 76.59332798398151 6.426285302231534 11.127527835656085 5.85285887813087 10 42.942346087732105 24.311243576466026 18.019403856625328 14.727006479176538 11 31.718973417225627 24.632533202802577 24.161111307308765 19.48738207266303 12 27.91721043074328 22.305893677115424 29.227664745115977 20.54923114702532 13 25.102312513294674 23.09991926694717 31.416491389158775 20.381276830599386 14 22.22713172628292 23.90716766604276 32.54174424458748 21.323956363086843 15 21.953066418745838 24.2002869595998 32.50224007529609 21.344406546358265 16 26.018217910254084 24.211785054611845 29.493845644644807 20.276151390489268 17 25.17688587237279 24.569704326486765 28.065207339398306 22.188202461742144 18 26.145025472386912 23.185990721037324 29.434548326082687 21.234435480493072 19 24.69790808587106 25.547124531760613 27.388790835689758 22.36617654667857 20 26.158494669401023 26.621703639886235 27.04278030507731 20.17702138563543 21 25.91572060614672 25.815605050291847 28.586317431194168 19.68235691236727 22 25.546056851509498 22.70619164203474 29.207707337345074 22.540044169110697 23 24.149284695296373 24.57643892499382 29.391841116037952 21.88243526367185 24 24.185667953084486 24.061981302454928 29.622460050279525 22.12989069418106 25 23.355998268715407 25.416867541124166 28.32637835467188 22.900755835488543 26 22.890900325478217 25.607818047574188 27.83401350040613 23.66726812654146 27 23.429257559792145 24.724271575148673 28.79919644741716 23.04727441764202 28 21.355493995119883 25.797290227522662 28.374423965972206 24.47279181138525 29 23.230504774583956 24.850586361780987 27.149794717939407 24.769114145695646 30 25.500146600711403 23.47286819158783 27.832124527654152 23.194860680046617 31 25.39239302459853 24.62210278804165 27.574402940884184 22.411101246475628 32 25.807720642283588 24.038163819929977 27.109633514647342 23.044482023139096 33 25.636481155854213 23.963261943851517 27.35142202690061 23.048834873393652 34 23.519517605636693 24.6124115365315 29.40350346955017 22.464567388281633 35 24.091712090986068 25.88648259311609 28.101179950935983 21.920625364961854 36 25.08046613277179 26.950220640230356 26.55041545081156 21.418897776186295 37 23.88523915626979 26.548033702559064 27.586393811396746 21.9803333297744 38 23.67958751405437 26.551975906563197 28.059376162642195 21.709060416740243 39 24.467289151629483 25.694628664915125 26.62695991189174 23.21112227156365 40 25.320201413772914 24.406677765065997 27.856927561180132 22.41619325998096 41 21.84810523713589 25.232158857680687 28.346746408693217 24.572989496490205 42 22.28010509258841 25.10066992829295 28.951956852577176 23.66726812654146 43 23.16972912952029 24.16406796031186 28.86908843924037 23.79711447092748 44 23.994142541883864 23.871195054505076 27.205560478747827 24.929101924863232 45 23.960305290848417 23.289884222396154 27.076042651362155 25.673767835393274 46 23.973445970862183 23.93648780832347 27.45474062350884 24.635325597305506 47 21.710456613991703 24.95792929164343 29.733580925645924 23.598033168718942 48 22.472451796289896 24.55122524521741 29.910980105831754 23.065342852660947 49 24.27978807368308 23.99340337863309 28.878861820000605 22.847946727683226 50 22.755058545835922 23.862489353995958 29.590676030496233 23.791776069671887 51 22.33923815265035 23.534793646152696 28.093788318428242 26.03217988276871 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 482.0 1 1017.5 2 1553.0 3 6874.0 4 12195.0 5 7731.0 6 3267.0 7 3092.0 8 2917.0 9 2988.0 10 3059.0 11 3028.5 12 2998.0 13 2969.5 14 2941.0 15 2880.5 16 2820.0 17 2765.5 18 2711.0 19 2892.5 20 3074.0 21 3035.0 22 2996.0 23 3516.0 24 4036.0 25 4463.0 26 6415.5 27 7941.0 28 9636.5 29 11332.0 30 13742.5 31 16153.0 32 17961.0 33 19769.0 34 23720.0 35 27671.0 36 30046.5 37 32422.0 38 34576.5 39 36731.0 40 40082.5 41 43434.0 42 48895.0 43 54356.0 44 60845.0 45 67334.0 46 75628.0 47 83922.0 48 95958.5 49 107995.0 50 110890.5 51 113786.0 52 105457.5 53 97129.0 54 89808.5 55 82488.0 56 79201.5 57 75915.0 58 72638.0 59 69361.0 60 63003.0 61 56645.0 62 51509.0 63 46373.0 64 41193.0 65 36013.0 66 30928.0 67 25843.0 68 22701.5 69 19560.0 70 16442.5 71 13325.0 72 11226.5 73 9128.0 74 7680.0 75 4846.0 76 3460.0 77 2704.0 78 1948.0 79 1428.0 80 908.0 81 576.0 82 244.0 83 192.5 84 141.0 85 102.0 86 63.0 87 41.0 88 19.0 89 13.5 90 8.0 91 5.5 92 3.0 93 2.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1217593.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.773283913415547 #Duplication Level Percentage of deduplicated Percentage of total 1 70.73785879115762 20.35360494425076 2 10.231291005547066 5.887756818067611 3 3.8172192398220464 3.2950179884145605 4 1.9983406631914824 2.299952930309266 5 1.2919697949082216 1.858710685822576 6 0.9888700292866152 1.7071822863678796 7 0.7835221103512002 1.5781152893501516 8 0.6812621963206023 1.5681720475367782 9 0.5691896180324817 1.4739709032195432 >10 8.735172961264826 53.22057536664829 >50 0.12124298314834706 2.243851124617359 >100 0.03830105050745343 2.157111996247818 >500 0.003167756054110884 0.6324755448581629 >1k 0.0023038225848079157 1.0525791754661658 >5k 2.8797782310098946E-4 0.6709228988230768 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8097 0.665000537946588 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 2696 0.22142045823193793 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC 1823 0.14972162290683338 No Hit CGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTG 1702 0.13978398364642372 RNA PCR Primer, Index 30 (95% over 22bp) CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC 1690 0.13879843264539135 RNA PCR Primer, Index 30 (95% over 23bp) CGTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTT 1368 0.11235281411768956 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.2851700034412155E-4 0.0 0.0 0.10167601160650562 0.0 2 3.2851700034412155E-4 0.0 0.0 0.40768959742705485 0.0 3 3.2851700034412155E-4 0.0 0.0 0.7120605982458835 0.0 4 3.2851700034412155E-4 0.0 0.0 1.0770429856282024 0.0 5 3.2851700034412155E-4 0.0 0.0 2.118688264469326 0.0 6 3.2851700034412155E-4 0.0 0.0 3.087402769234054 0.0 7 3.2851700034412155E-4 0.0 0.0 3.785829911965657 0.0 8 3.2851700034412155E-4 0.0 0.0 4.725634920700102 0.0 9 3.2851700034412155E-4 0.0 0.0 5.2184104212162845 0.0 10 3.2851700034412155E-4 0.0 0.0 6.008740194794155 0.0 11 3.2851700034412155E-4 0.0 0.0 7.128654648967266 0.0 12 4.1064625043015196E-4 0.0 0.0 7.916109898792125 0.0 13 4.1064625043015196E-4 0.0 0.0 8.29981775519406 0.0 14 4.927755005161823E-4 0.0 0.0 8.525508934430471 0.0 15 4.927755005161823E-4 0.0 0.0 8.816903513735706 0.0 16 6.570340006882431E-4 0.0 0.0 9.36347367305824 0.0 17 6.570340006882431E-4 0.0 0.0 9.954147239676969 0.0 18 6.570340006882431E-4 0.0 0.0 10.541946282542689 0.0 19 7.391632507742735E-4 0.0 0.0 10.967129410238067 0.0 20 7.391632507742735E-4 0.0 0.0 11.391573374682674 0.0 21 7.391632507742735E-4 0.0 0.0 11.901678146967008 0.0 22 7.391632507742735E-4 0.0 0.0 12.455229292546854 0.0 23 7.391632507742735E-4 0.0 0.0 12.992847363610007 0.0 24 7.391632507742735E-4 0.0 0.0 13.461887510851327 0.0 25 7.391632507742735E-4 0.0 0.0 13.894298012554277 0.0 26 7.391632507742735E-4 0.0 0.0 14.296484950225569 0.0 27 7.391632507742735E-4 0.0 0.0 14.723803438423184 0.0 28 7.391632507742735E-4 0.0 0.0 15.131410906600152 0.0 29 7.391632507742735E-4 0.0 0.0 15.56718870755663 0.0 30 7.391632507742735E-4 0.0 0.0 16.220937538241433 0.0 31 9.034217509463343E-4 0.0 0.0 16.72069402501493 0.0 32 9.034217509463343E-4 0.0 0.0 17.245664191564835 0.0 33 9.034217509463343E-4 0.0 0.0 17.706409284547465 0.0 34 9.855510010323646E-4 0.0 0.0 18.16436198302717 0.0 35 9.855510010323646E-4 0.0 0.0 18.643011252528556 0.0 36 0.001067680251118395 0.0 0.0 19.089301597496043 0.0 37 0.0011498095012044255 0.0 0.0 19.54183376547007 0.0 38 0.0011498095012044255 0.0 0.0 20.0390442454909 0.0 39 0.0011498095012044255 0.0 0.0 20.716856946450907 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGTAG 50 2.1827873E-11 45.0 1 CGTTTTT 3470 0.0 41.10951 1 TATACGG 105 0.0 40.714287 2 GCGTTAG 50 1.0822987E-9 40.5 1 GTATACG 50 1.0822987E-9 40.5 1 TTACGAG 45 1.9284926E-8 40.000004 1 TATCACG 35 6.248594E-6 38.571426 1 TACGTCG 35 6.248594E-6 38.571426 24 TACGTAG 60 1.5643309E-10 37.499996 1 TACGGGA 295 0.0 37.372883 4 GCGATGA 270 0.0 36.666668 9 TTACACG 955 0.0 36.518326 34 AGGGCGA 975 0.0 36.461536 6 ACCGGTA 75 1.8189894E-12 36.0 41 TCGGAAT 25 0.0021071574 36.0 19 CGTAGCA 25 0.0021071574 36.0 14 GCGTAAC 25 0.0021071574 36.0 32 CACAACG 460 0.0 35.70652 12 ACACGCG 985 0.0 35.40609 36 TACACGC 985 0.0 35.40609 35 >>END_MODULE