##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934540.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	618640
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.079155890340104	33.0	31.0	34.0	30.0	34.0
2	32.20673574291995	33.0	31.0	34.0	30.0	34.0
3	32.254196301564726	34.0	31.0	34.0	30.0	34.0
4	35.8310261218156	37.0	35.0	37.0	35.0	37.0
5	35.86030647872753	37.0	35.0	37.0	35.0	37.0
6	35.89121298331825	37.0	35.0	37.0	35.0	37.0
7	36.096618388723655	37.0	35.0	37.0	35.0	37.0
8	35.80293547135653	37.0	35.0	37.0	35.0	37.0
9	37.54000387947757	39.0	37.0	39.0	35.0	39.0
10	37.145709944394156	39.0	37.0	39.0	33.0	39.0
11	37.13252780292254	39.0	37.0	39.0	34.0	39.0
12	37.077308289150395	39.0	37.0	39.0	34.0	39.0
13	37.04791963015647	39.0	37.0	39.0	33.0	39.0
14	38.234013319539635	40.0	37.0	41.0	34.0	41.0
15	38.37171860856071	40.0	38.0	41.0	34.0	41.0
16	38.48018395189448	40.0	38.0	41.0	34.0	41.0
17	38.45535044613992	40.0	38.0	41.0	34.0	41.0
18	38.45878540023277	40.0	37.0	41.0	34.0	41.0
19	38.442231346178716	40.0	37.0	41.0	34.0	41.0
20	38.305027156342945	40.0	37.0	41.0	34.0	41.0
21	38.31430395706712	40.0	37.0	41.0	34.0	41.0
22	38.29896870554765	40.0	37.0	41.0	34.0	41.0
23	38.32570315530842	40.0	37.0	41.0	34.0	41.0
24	38.23950439674124	40.0	37.0	41.0	34.0	41.0
25	38.1579480796586	40.0	37.0	41.0	34.0	41.0
26	38.04023988102936	40.0	37.0	41.0	34.0	41.0
27	37.98688736583473	40.0	36.0	41.0	34.0	41.0
28	38.012988167593434	40.0	37.0	41.0	34.0	41.0
29	38.03307739557739	40.0	37.0	41.0	34.0	41.0
30	37.932141471615154	40.0	36.0	41.0	34.0	41.0
31	37.94952314754946	40.0	36.0	41.0	34.0	41.0
32	37.87632225526962	40.0	36.0	41.0	34.0	41.0
33	37.85949987068408	40.0	36.0	41.0	34.0	41.0
34	37.81729115479116	40.0	36.0	41.0	33.0	41.0
35	37.80086964955386	40.0	36.0	41.0	33.0	41.0
36	37.70597924479503	40.0	36.0	41.0	33.0	41.0
37	37.67097989137463	40.0	36.0	41.0	33.0	41.0
38	37.59351803957067	40.0	36.0	41.0	33.0	41.0
39	37.531467412388466	40.0	36.0	41.0	33.0	41.0
40	37.43845855424803	40.0	35.0	41.0	33.0	41.0
41	37.35353840682788	40.0	35.0	41.0	33.0	41.0
42	37.346655567050306	40.0	35.0	41.0	33.0	41.0
43	37.289969934048884	39.0	35.0	41.0	33.0	41.0
44	37.245553148842625	39.0	35.0	41.0	33.0	41.0
45	37.15180880641407	39.0	35.0	41.0	33.0	41.0
46	37.08834055347213	39.0	35.0	41.0	32.0	41.0
47	37.01549043062201	39.0	35.0	41.0	32.0	41.0
48	36.97901364282943	39.0	35.0	41.0	32.0	41.0
49	36.952743113927326	39.0	35.0	41.0	32.0	41.0
50	36.82701247898616	39.0	35.0	41.0	32.0	41.0
51	35.87836706323549	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	1.0
9	9.0
10	9.0
11	4.0
12	10.0
13	10.0
14	14.0
15	18.0
16	28.0
17	37.0
18	97.0
19	162.0
20	272.0
21	548.0
22	849.0
23	1268.0
24	1816.0
25	2502.0
26	3297.0
27	3880.0
28	4642.0
29	5780.0
30	7340.0
31	9658.0
32	12889.0
33	18803.0
34	36576.0
35	50119.0
36	43660.0
37	65931.0
38	120928.0
39	227237.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46204577783525	21.353937669727145	24.334023018233545	9.84999353420406
2	33.36528514160093	25.306155437734386	23.02760894866158	18.3009504720031
3	31.593010474589423	25.1952670373723	25.815498512866935	17.396223975171342
4	28.616804603646713	24.498415879994827	26.98144316565369	19.903336350704773
5	25.62378766326135	29.71178714599767	23.88545842493211	20.77896676580887
6	26.58024052760895	35.1243049269365	23.267813267813267	15.027641277641276
7	82.02589551273762	4.420179749127118	9.03643476011897	4.517489978016294
8	80.92800336221389	5.104260959524117	8.39066339066339	5.577072287598604
9	74.50892279839648	6.713597568860727	10.84265485581275	7.93482477693004
10	39.42761541445752	26.333247122720806	18.966604163972587	15.272533298849089
11	29.437475753265225	24.1691452217768	25.39813138497349	20.995247639984484
12	27.554474330790118	20.726432173800593	30.075973102288895	21.643120393120395
13	23.84181430234062	25.21434113539377	31.292835898099057	19.651008664166557
14	20.27851416009311	27.562718220612958	29.26079787921893	22.897969740075002
15	18.634585542480277	26.837417561101773	33.62666494245442	20.901331953963535
16	21.307545583861373	25.36644898487004	31.110015517910256	22.21598991335833
17	20.76377214535109	25.59242855295487	27.841394025604554	25.802405276089484
18	21.44623690676322	24.86842105263158	30.97326393379025	22.71207810681495
19	21.86602870813397	27.864347601189703	27.329787921893185	22.939835768783137
20	23.651881546618387	26.551144445881285	28.88044743307901	20.91652657442131
21	22.53572352256563	28.25423509634036	29.079432303116516	20.1306090779775
22	21.093689383163067	24.675417043838095	27.398487003750162	26.832406569248672
23	20.557351609983186	27.115608431397902	28.271207810681492	24.055832147937412
24	22.085542480279322	24.180460364670893	29.8010151299625	23.932982025087288
25	19.55482994956679	27.824421311263414	27.537016681753524	25.08373205741627
26	19.87100737100737	27.03122979438769	29.31009957325747	23.787663261347472
27	21.50944006207164	26.30770722875986	28.012575973102287	24.17027673606621
28	17.617192551403075	26.23205741626794	31.023373852321225	25.12737618000776
29	19.437960687960686	24.513610500452607	30.539247381352645	25.50918143023406
30	21.61095305832148	25.868356394672183	28.35510151299625	24.165589034010086
31	22.142603129445234	26.36121169015906	27.177033492822964	24.31915168757274
32	22.279193068666753	28.209782749256433	26.636654597180915	22.874369584895902
33	23.626503297555928	26.294129057286952	25.24424544161386	24.835122203543257
34	22.236033880770723	24.887333505754558	28.784753653174704	24.09187896030001
35	23.15126729600414	24.626923574291997	27.36033880770723	24.861470321996638
36	23.34524117418854	26.85600672442778	27.045454545454543	22.753297555929137
37	21.355069184016553	26.906278287857234	29.294581663002717	22.444070865123496
38	22.15375662744084	27.633841975947238	26.466604163972583	23.745797232639337
39	24.739751713435922	26.218964179490495	26.52883744989008	22.5124466571835
40	26.497963274279062	23.00514030777189	28.579302987197725	21.917593430751324
41	21.965763610500453	24.731507823613086	27.56449631449631	25.738232251390148
42	21.866836932626406	24.646805896805894	29.18757274020432	24.298784430363376
43	21.40534074744601	24.85548946075262	29.276962369067633	24.46220742273374
44	21.052793223845857	24.8495085995086	27.915750678908573	26.181947497736974
45	22.844950213371266	23.151105651105652	26.627764127764124	27.376180007758954
46	24.973975171343593	24.578430104745895	26.72798396482607	23.719610759084446
47	21.165298073192808	24.076684339842235	31.20861890598733	23.549398680977628
48	21.604325617483514	23.441743178585284	29.658444329496962	25.295486874434243
49	22.631255657571447	22.692357429199532	30.74987068408121	23.926516229147808
50	21.397096857623175	22.921569895254105	29.64195654985129	26.039376697271432
51	21.45076296392086	22.631255657571447	26.348441743178586	29.56953963532911
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	198.0
1	452.5
2	707.0
3	1339.5
4	1972.0
5	1342.0
6	712.0
7	727.5
8	743.0
9	746.0
10	749.0
11	775.0
12	801.0
13	818.0
14	835.0
15	888.0
16	941.0
17	974.5
18	1008.0
19	1116.0
20	1224.0
21	1321.0
22	1418.0
23	1657.5
24	1897.0
25	2076.0
26	2855.5
27	3456.0
28	4272.5
29	5089.0
30	6205.0
31	7321.0
32	8528.0
33	9735.0
34	11869.5
35	14004.0
36	14837.0
37	15670.0
38	17873.5
39	20077.0
40	22887.5
41	25698.0
42	30069.0
43	34440.0
44	38968.0
45	43496.0
46	54857.0
47	66218.0
48	71014.5
49	75811.0
50	74405.0
51	72999.0
52	62534.0
53	52069.0
54	44207.0
55	36345.0
56	31811.0
57	27277.0
58	25215.0
59	23153.0
60	21655.0
61	20157.0
62	18158.0
63	16159.0
64	13823.0
65	11487.0
66	9522.5
67	7558.0
68	6312.0
69	5066.0
70	4500.5
71	3935.0
72	3135.0
73	2335.0
74	1977.5
75	1388.5
76	1157.0
77	855.0
78	553.0
79	384.0
80	215.0
81	141.5
82	68.0
83	37.5
84	7.0
85	4.5
86	2.0
87	1.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	618640.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.63243136458089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.97103985596924	25.645031379053396
2	12.037272896265376	8.578346005858116
3	4.431583971356914	4.73724335087256
4	2.1342109881459654	3.0418850621058184
5	1.279263221193901	2.2791629463212164
6	0.903022560212489	1.9306133638463772
7	0.6618327853811885	1.6507897909947211
8	0.5259662565577866	1.4993165229504695
9	0.4475955621776797	1.435402633354845
>10	5.4520848130916475	40.888261326640745
>50	0.10072715461422968	2.3849924497713646
>100	0.04990572660050005	3.3618947540510815
>500	0.002289253513784406	0.5217323930438859
>1k	0.003204954919298168	2.045328021135459
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	2378	0.3843915686020949	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	2301	0.37194491141859565	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTC	1900	0.3071253071253071	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	1781	0.28788956420535367	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG	1565	0.2529742661321609	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCT	1478	0.23891115996379156	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1134	0.18330531488426224	No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	746	0.1205870942713048	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	678	0.10959524117418855	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG	673	0.10878701668175352	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2457002457002457	0.0
2	0.0	0.0	0.0	1.246443812233286	0.0
3	0.0	0.0	0.0	1.762575973102289	0.0
4	0.0	0.0	0.0	2.5379865511444457	0.0
5	0.0	0.0	0.0	4.971065563170827	0.0
6	0.0	0.0	0.0	6.202314754946334	0.0
7	0.0	0.0	0.0	7.343689383163067	0.0
8	0.0	0.0	0.0	9.153304021725074	0.0
9	0.0	0.0	0.0	9.725203672572095	0.0
10	0.0	0.0	0.0	11.497963274279064	0.0
11	0.0	0.0	0.0	13.842460881934565	0.0
12	0.0	0.0	0.0	15.757629639208586	0.0
13	0.0	0.0	0.0	16.46304797620587	0.0
14	0.0	0.0	0.0	16.72426613216087	0.0
15	0.0	0.0	0.0	17.309259019785337	0.0
16	0.0	0.0	0.0	18.474557092978145	0.0
17	0.0	0.0	0.0	19.927744730376308	0.0
18	0.0	0.0	0.0	21.37414328203802	0.0
19	0.0	0.0	0.0	22.329141342299238	0.0
20	1.6164489848700375E-4	0.0	0.0	23.189253847148585	0.0
21	1.6164489848700375E-4	0.0	0.0	24.300562524246736	0.0
22	1.6164489848700375E-4	0.0	0.0	25.46602224233803	0.0
23	1.6164489848700375E-4	0.0	0.0	26.74431009957326	0.0
24	1.6164489848700375E-4	0.0	0.0	27.6555023923445	0.0
25	1.6164489848700375E-4	0.0	0.0	28.533395836027417	0.0
26	1.6164489848700375E-4	0.0	0.0	29.281326781326783	0.0
27	1.6164489848700375E-4	0.0	0.0	30.03572352256563	0.0
28	3.232897969740075E-4	0.0	0.0	30.793838096469674	0.0
29	3.232897969740075E-4	0.0	0.0	31.555670503038925	0.0
30	3.232897969740075E-4	0.0	0.0	32.40349799560326	0.0
31	3.232897969740075E-4	0.0	0.0	33.212369067632224	0.0
32	3.232897969740075E-4	0.0	0.0	33.93249709039183	0.0
33	3.232897969740075E-4	0.0	0.0	34.69416785206259	0.0
34	3.232897969740075E-4	0.0	0.0	35.399586189059875	0.0
35	3.232897969740075E-4	0.0	0.0	36.184857105909735	0.0
36	3.232897969740075E-4	0.0	0.0	36.898842622526836	0.0
37	3.232897969740075E-4	0.0	0.0	37.63966119229277	0.0
38	3.232897969740075E-4	0.0	0.0	38.33004655373077	0.0
39	3.232897969740075E-4	0.0	0.0	39.027059356006724	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCTT	20	7.0307567E-4	45.000004	23
CGCGACA	20	7.0307567E-4	45.000004	38
TACGAAT	95	0.0	45.000004	12
ATAACGG	20	7.0307567E-4	45.000004	2
TCGAGAG	20	7.0307567E-4	45.000004	1
TCGCGAC	20	7.0307567E-4	45.000004	37
TCGTTAG	25	3.8887094E-5	45.0	1
GAATGCG	25	3.8887094E-5	45.0	1
TCGATTG	35	1.2107193E-7	45.0	1
CCTCGAT	25	3.8887094E-5	45.0	15
ACGATTG	35	1.2107193E-7	45.0	1
TGTTACG	35	1.2107193E-7	45.0	1
AGTTACG	25	3.8887094E-5	45.0	1
ATCGGGC	25	3.8887094E-5	45.0	4
GCTAACG	25	3.8887094E-5	45.0	1
AGGGCGT	35	1.2107193E-7	45.0	6
CGATCGA	25	3.8887094E-5	45.0	41
GGACGAT	30	2.1638698E-6	44.999996	8
AACACGT	180	0.0	42.5	41
TAACGGG	140	0.0	41.785713	3
>>END_MODULE