##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934536.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	261615
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00078359421287	33.0	31.0	34.0	30.0	34.0
2	32.110934006077635	33.0	31.0	34.0	30.0	34.0
3	32.17777650364085	34.0	31.0	34.0	30.0	34.0
4	35.77951570055234	37.0	35.0	37.0	33.0	37.0
5	35.825724824646905	37.0	35.0	37.0	35.0	37.0
6	35.83310207748027	37.0	35.0	37.0	35.0	37.0
7	36.04902241843931	37.0	35.0	37.0	35.0	37.0
8	35.767306920474745	37.0	35.0	37.0	35.0	37.0
9	37.495789614509874	39.0	37.0	39.0	35.0	39.0
10	37.195225808917684	39.0	37.0	39.0	34.0	39.0
11	37.07825239378476	39.0	37.0	39.0	34.0	39.0
12	36.75569825889188	39.0	35.0	39.0	33.0	39.0
13	36.56862947460964	39.0	35.0	39.0	33.0	39.0
14	37.597679796647746	40.0	35.0	41.0	33.0	41.0
15	37.84922500621142	40.0	36.0	41.0	33.0	41.0
16	37.99428167345145	40.0	36.0	41.0	34.0	41.0
17	37.99073065382337	40.0	36.0	41.0	34.0	41.0
18	38.0120673508782	40.0	36.0	41.0	34.0	41.0
19	38.001586300479715	40.0	36.0	41.0	34.0	41.0
20	37.88011773025247	40.0	35.0	41.0	34.0	41.0
21	37.82616822429907	40.0	35.0	41.0	34.0	41.0
22	37.83039198822698	40.0	35.0	41.0	34.0	41.0
23	37.87307302715823	40.0	35.0	41.0	34.0	41.0
24	37.75257534927279	40.0	35.0	41.0	34.0	41.0
25	37.67381075244157	39.0	35.0	41.0	34.0	41.0
26	37.546799686562316	39.0	35.0	41.0	33.0	41.0
27	37.471100663188274	39.0	35.0	41.0	33.0	41.0
28	37.50945855551096	39.0	35.0	41.0	33.0	41.0
29	37.531529155438335	39.0	35.0	41.0	33.0	41.0
30	37.395879441163544	39.0	35.0	41.0	33.0	41.0
31	37.40387592454561	39.0	35.0	41.0	33.0	41.0
32	37.31176346922004	39.0	35.0	41.0	33.0	41.0
33	37.252875408520154	39.0	35.0	41.0	33.0	41.0
34	37.20855837776886	39.0	35.0	41.0	33.0	41.0
35	37.214838598704205	39.0	35.0	41.0	33.0	41.0
36	37.14000726258051	39.0	35.0	41.0	33.0	41.0
37	37.085163312501194	39.0	35.0	41.0	33.0	41.0
38	36.97201613057355	39.0	35.0	41.0	32.0	41.0
39	36.94016398142308	39.0	35.0	41.0	32.0	41.0
40	36.89268581694475	39.0	35.0	41.0	32.0	41.0
41	36.82848842765132	39.0	35.0	41.0	32.0	41.0
42	36.83070924832292	39.0	35.0	41.0	32.0	41.0
43	36.765196949716184	39.0	35.0	41.0	32.0	41.0
44	36.665982455134454	39.0	35.0	41.0	32.0	41.0
45	36.57079678153011	38.0	35.0	41.0	32.0	41.0
46	36.50371729449764	38.0	35.0	40.0	31.0	41.0
47	36.44982512470615	38.0	35.0	40.0	31.0	41.0
48	36.42338550924068	38.0	35.0	40.0	31.0	41.0
49	36.410087342086655	38.0	35.0	40.0	31.0	41.0
50	36.24545993157885	38.0	35.0	40.0	31.0	41.0
51	35.30362937904937	36.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	7.0
10	5.0
11	3.0
12	3.0
13	1.0
14	9.0
15	12.0
16	12.0
17	20.0
18	33.0
19	73.0
20	163.0
21	216.0
22	368.0
23	548.0
24	812.0
25	1150.0
26	1565.0
27	1972.0
28	2355.0
29	2973.0
30	3736.0
31	4895.0
32	6684.0
33	9769.0
34	21048.0
35	30289.0
36	18082.0
37	26815.0
38	47822.0
39	80105.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.567398658333815	20.394090552911724	23.70544502417675	11.33306576457772
2	33.81725053991552	22.793035567532442	24.367104332702635	19.022609559849396
3	31.963381304588808	22.609942090476466	26.373105517649982	19.05357108728475
4	30.422949754410105	25.61932610897693	23.874777822372568	20.08294631424039
5	25.954933776732986	29.43753225159108	24.319706438850982	20.287827532824952
6	27.104332702635553	32.77831928597366	24.606005007358142	15.511343004032643
7	80.38644573132274	4.9079754601226995	9.573992316954303	5.131586491600252
8	78.46988895896642	4.409533092521453	10.239474036274679	6.881103912237448
9	72.35212048238824	6.08833591346062	13.64906446495805	7.910479139193089
10	45.746230147353934	20.13454885996598	19.565009651587257	14.554211341092827
11	34.319897559390704	21.695621428434915	23.555224279953364	20.429256732221013
12	30.994782409265525	20.01911205397244	29.538061655486114	19.448043881275918
13	23.10303308296543	27.295453242359958	30.273111251266172	19.328402423408445
14	18.81008351967586	29.128681459396443	30.047971255470824	22.013263765456873
15	16.955832043269687	24.180952927011067	38.93545859373507	19.927756435984175
16	19.91667144468016	23.174894405901803	34.15820958278386	22.750224566634174
17	19.47365403359899	22.70320891386197	27.819505762284276	30.003631290254763
18	20.377271945415973	23.505150698545574	33.64371308984577	22.473864266192688
19	21.877568182252546	24.482923379775627	29.09733769088164	24.54217074709019
20	24.214207900923114	25.6449362613	28.85652581082889	21.284330026947995
21	21.637520784358696	27.31150736769681	30.448559906733173	20.602411941211322
22	20.24960342488007	25.44961106970166	27.557288381782392	26.74349712363588
23	19.625785983219618	25.399537488293866	28.985723295682586	25.98895323280393
24	22.596181411616307	22.605355197523078	31.38428606922386	23.414177321636757
25	19.14569118743191	24.687422357280735	29.864877778414844	26.3020086768725
26	19.001204059400266	26.461403207002654	28.184928234237333	26.352464499359744
27	22.349635915371824	25.60288974256063	28.18301702884009	23.86445731322745
28	17.977562448636355	25.49165758844103	30.860998031458443	25.669781931464176
29	20.126904038377006	23.46845555491849	29.557938191617456	26.846702215087053
30	23.159604762723852	26.526766431588406	27.241175009078226	23.072453796609523
31	24.60638724843759	26.48586663608738	23.59574183437494	25.31200428110009
32	24.287215947097835	29.139766450700456	22.591212277583473	23.981805324618236
33	24.51426714829043	28.70821627200275	23.19171301339755	23.58580356630927
34	20.075683733730866	25.497391204632763	26.24925940790857	28.177665653727807
35	19.26495040421994	28.05534850830419	25.48210156145481	27.19759952602106
36	24.86592894138333	27.64023469602278	24.636966534793494	22.856869827800395
37	22.525466811918278	27.048907746115475	27.71171377787971	22.713911664086538
38	20.392561588593928	31.518070447030944	23.90344590333123	24.1859220610439
39	21.0102631729832	28.953232803929442	26.63723410354911	23.399269919538252
40	21.658926284807826	24.800183475718136	26.794717428282016	26.74617281119202
41	18.596028515184525	25.49089310628213	25.13464442023584	30.778433958297498
42	21.204823882422644	25.199625403742136	26.850142384802094	26.745408329033122
43	22.914588230797165	24.78336486822239	26.872312367410128	25.429734533570326
44	21.076390879727843	24.941994916193643	26.374634481967778	27.606979722110736
45	20.829463142403913	25.283718441220877	25.060489650822777	28.826328765552432
46	23.777306347113125	25.117825812740097	26.811536035777767	24.293331804369014
47	19.28215125279514	24.969134032834507	29.37943160751486	26.369283106855494
48	19.565774133746153	24.037230281138314	29.6875179175506	26.709477667564936
49	21.468187986162874	21.97045276455861	31.35867591690079	25.202683332377728
50	20.777860596678323	22.920704088068344	29.20360071096841	27.09783460428492
51	18.472564646522564	24.3621351986698	25.597920608527797	31.567379546279838
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	134.0
1	268.0
2	402.0
3	618.5
4	835.0
5	589.5
6	344.0
7	346.0
8	348.0
9	385.0
10	422.0
11	394.0
12	366.0
13	442.0
14	518.0
15	541.5
16	565.0
17	533.0
18	501.0
19	530.5
20	560.0
21	547.5
22	535.0
23	587.5
24	640.0
25	834.0
26	1056.5
27	1085.0
28	1719.0
29	2353.0
30	2520.5
31	2688.0
32	2843.0
33	2998.0
34	3800.5
35	4603.0
36	4699.5
37	4796.0
38	5653.0
39	6510.0
40	8016.5
41	9523.0
42	11753.5
43	13984.0
44	16107.5
45	18231.0
46	21623.0
47	25015.0
48	28825.0
49	32635.0
50	33670.5
51	34706.0
52	29076.5
53	23447.0
54	20372.0
55	17297.0
56	14935.5
57	12574.0
58	11557.5
59	10541.0
60	9541.0
61	8541.0
62	7901.0
63	7261.0
64	6279.5
65	5298.0
66	4450.5
67	3603.0
68	2969.0
69	2335.0
70	2128.5
71	1922.0
72	1467.0
73	1012.0
74	877.0
75	532.0
76	322.0
77	258.0
78	194.0
79	167.5
80	141.0
81	92.5
82	44.0
83	25.5
84	7.0
85	4.0
86	1.0
87	2.5
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	261615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.92930451235595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.33124390583647	22.501003382833552
2	13.230719227376142	8.713567647115035
3	5.353577564191856	5.288687575253713
4	2.769652226401077	3.648108862259427
5	1.5717137948646513	2.5877721078684326
6	0.9936388540650973	1.96319018404908
7	0.6221850768445001	1.434168530091929
8	0.5386079769698658	1.4188788869139766
9	0.4260110507498723	1.262542285419414
>10	5.990852950735943	43.71117864036848
>50	0.11956168454287969	2.64969516273914
>100	0.046431722152574635	2.7670431741299235
>500	0.0034823791614430975	0.7874166236645451
>1k	0.002321586107628732	1.2667469372933509
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	1825	0.6975899699940753	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	1489	0.5691569672992757	No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	957	0.3658047130325096	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	589	0.2251399957953481	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	514	0.1964719148366875	No Hit
ACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	441	0.1685683160369245	No Hit
AATGACTGGGGAAATTATAAAAGAGGATTCTGACCGTAGGTATTTTGTACA	386	0.14754505666724002	No Hit
GCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	374	0.1429581637138543	No Hit
GGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	304	0.11620128815243774	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	283	0.10817422548401276	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC	283	0.10817422548401276	No Hit
GGCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	266	0.10167612713338303	No Hit
ACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	263	0.1005294038950366	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3780364275748715	0.0
2	0.0	0.0	0.0	2.106530588842383	0.0
3	0.0	0.0	0.0	2.9191751237505494	0.0
4	0.0	0.0	0.0	3.925233644859813	0.0
5	0.0	0.0	0.0	6.628060317642337	0.0
6	0.0	0.0	0.0	7.83632436978002	0.0
7	0.0	0.0	0.0	9.191368996426046	0.0
8	0.0	0.0	0.0	11.245532557383942	0.0
9	0.0	0.0	0.0	11.93891787550408	0.0
10	0.0	0.0	0.0	13.526747319534431	0.0
11	0.0	0.0	0.0	15.981881772834127	0.0
12	0.0	0.0	0.0	17.765036408462816	0.0
13	0.0	0.0	0.0	18.5245494333276	0.0
14	0.0	0.0	0.0	18.81963954666208	0.0
15	0.0	0.0	0.0	19.405615121457103	0.0
16	0.0	0.0	0.0	20.642165013474	0.0
17	0.0	0.0	0.0	22.06868872197695	0.0
18	0.0	0.0	0.0	23.516617930929037	0.0
19	0.0	0.0	0.0	24.414502226554287	0.0
20	0.0	0.0	0.0	25.332263058310875	0.0
21	0.0	0.0	0.0	26.422414616898877	0.0
22	3.822410794488084E-4	0.0	0.0	27.51868203275806	0.0
23	3.822410794488084E-4	0.0	0.0	28.60309997515433	0.0
24	3.822410794488084E-4	0.0	0.0	29.52009632475202	0.0
25	3.822410794488084E-4	0.0	0.0	30.359497735221606	0.0
26	3.822410794488084E-4	0.0	0.0	31.05746994629513	0.0
27	3.822410794488084E-4	0.0	0.0	31.74359268390574	0.0
28	3.822410794488084E-4	0.0	0.0	32.43277335015194	0.0
29	3.822410794488084E-4	0.0	0.0	33.13074556122547	0.0
30	3.822410794488084E-4	0.0	0.0	33.99193471322363	0.0
31	3.822410794488084E-4	0.0	0.0	34.748772050532274	0.0
32	3.822410794488084E-4	0.0	0.0	35.417693939567684	0.0
33	3.822410794488084E-4	0.0	0.0	36.07973548917302	0.0
34	7.644821588976168E-4	0.0	0.0	36.761653574909694	0.0
35	7.644821588976168E-4	0.0	0.0	37.57124018118227	0.0
36	7.644821588976168E-4	0.0	0.0	38.24589568640942	0.0
37	7.644821588976168E-4	0.0	0.0	38.92246239703381	0.0
38	7.644821588976168E-4	0.0	0.0	39.60438048277048	0.0
39	7.644821588976168E-4	0.0	0.0	40.27674254152094	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	45	3.8198777E-10	45.000004	20
ATCCTAA	35	1.2071177E-7	45.0	25
TCTGATC	20	7.0232875E-4	45.0	28
TTGGCCG	20	7.0232875E-4	45.0	1
GTACCAT	20	7.0232875E-4	45.0	33
GCGACTC	20	7.0232875E-4	45.0	22
CATATGT	20	7.0232875E-4	45.0	45
ATTAGCG	20	7.0232875E-4	45.0	1
TTAGCGG	35	1.2071177E-7	45.0	2
GACCGTA	35	1.2071177E-7	45.0	32
ATGTGAC	35	1.2071177E-7	45.0	29
ATAGCGA	20	7.0232875E-4	45.0	19
ATAGAAT	20	7.0232875E-4	45.0	10
CGACAAT	20	7.0232875E-4	45.0	20
CCTAACA	35	1.2071177E-7	45.0	27
CTGACCG	35	1.2071177E-7	45.0	30
TTAAGCA	20	7.0232875E-4	45.0	23
TAACACT	35	1.2071177E-7	45.0	29
TATTCGC	20	7.0232875E-4	45.0	12
ACGTGGT	20	7.0232875E-4	45.0	10
>>END_MODULE