##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934534.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	621897
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.240943435970905	33.0	31.0	34.0	30.0	34.0
2	32.36491734161766	34.0	31.0	34.0	30.0	34.0
3	32.413991384425394	34.0	31.0	34.0	30.0	34.0
4	35.950054430235234	37.0	35.0	37.0	35.0	37.0
5	35.95709257320746	37.0	35.0	37.0	35.0	37.0
6	35.97618094314653	37.0	35.0	37.0	35.0	37.0
7	36.15596634169324	37.0	36.0	37.0	35.0	37.0
8	35.96511962591876	37.0	36.0	37.0	35.0	37.0
9	37.70482411074503	39.0	38.0	39.0	35.0	39.0
10	37.331762333634025	39.0	37.0	39.0	34.0	39.0
11	37.2251466078788	39.0	37.0	39.0	34.0	39.0
12	37.01777143160363	39.0	37.0	39.0	34.0	39.0
13	36.97876658031796	39.0	37.0	39.0	33.0	39.0
14	38.02779881555949	40.0	37.0	41.0	33.0	41.0
15	38.20084997998061	40.0	37.0	41.0	33.0	41.0
16	38.34920895260791	40.0	37.0	41.0	34.0	41.0
17	38.35988917778989	40.0	37.0	41.0	34.0	41.0
18	38.32979416205578	40.0	37.0	41.0	34.0	41.0
19	38.30665689012811	40.0	37.0	41.0	34.0	41.0
20	38.18593271876211	40.0	37.0	41.0	34.0	41.0
21	38.13277118236621	40.0	36.0	41.0	34.0	41.0
22	38.14850690709233	40.0	36.0	41.0	34.0	41.0
23	38.16511898272544	40.0	36.0	41.0	34.0	41.0
24	38.087926135678416	40.0	36.0	41.0	34.0	41.0
25	37.996692378319885	40.0	36.0	41.0	34.0	41.0
26	37.840746940409744	40.0	35.0	41.0	34.0	41.0
27	37.80870465688048	40.0	35.0	41.0	34.0	41.0
28	37.81261527230394	40.0	35.0	41.0	34.0	41.0
29	37.789349361711025	40.0	35.0	41.0	34.0	41.0
30	37.682477966608616	40.0	35.0	41.0	33.0	41.0
31	37.663230406321304	40.0	35.0	41.0	33.0	41.0
32	37.563561168489315	40.0	35.0	41.0	33.0	41.0
33	37.52461098863638	40.0	35.0	41.0	33.0	41.0
34	37.41553665639165	40.0	35.0	41.0	33.0	41.0
35	37.37744031567929	40.0	35.0	41.0	33.0	41.0
36	37.29773419071004	40.0	35.0	41.0	33.0	41.0
37	37.25281356880641	40.0	35.0	41.0	33.0	41.0
38	37.165924582366536	39.0	35.0	41.0	33.0	41.0
39	37.08483880771253	39.0	35.0	41.0	33.0	41.0
40	36.990755703918815	39.0	35.0	41.0	32.0	41.0
41	36.89659702490927	39.0	35.0	41.0	32.0	41.0
42	36.884513030292794	39.0	35.0	41.0	32.0	41.0
43	36.826357097718756	39.0	35.0	41.0	32.0	41.0
44	36.73633415179684	39.0	35.0	41.0	32.0	41.0
45	36.5827122497777	39.0	35.0	41.0	32.0	41.0
46	36.5338794044673	38.0	35.0	41.0	32.0	41.0
47	36.486496960107544	38.0	35.0	41.0	31.0	41.0
48	36.42228375438376	38.0	35.0	41.0	31.0	41.0
49	36.37498170918978	38.0	35.0	41.0	31.0	41.0
50	36.26110272279815	38.0	35.0	40.0	31.0	41.0
51	35.34256798151463	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	9.0
10	10.0
11	14.0
12	9.0
13	9.0
14	21.0
15	28.0
16	47.0
17	74.0
18	120.0
19	194.0
20	321.0
21	566.0
22	876.0
23	1230.0
24	1991.0
25	2918.0
26	4020.0
27	5013.0
28	5681.0
29	6652.0
30	7951.0
31	10496.0
32	14006.0
33	21291.0
34	43652.0
35	51992.0
36	45432.0
37	68414.0
38	121522.0
39	207122.0
40	209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.104575194927776	19.954751349499997	23.592813600966075	11.347859854606149
2	34.46953434411164	22.393740442549166	24.300487058146285	18.836238155192902
3	34.66249234197946	23.226193405017227	25.065726318023724	17.045587934979586
4	29.129260954788332	25.67820716292248	24.951398704287044	20.241133178002148
5	25.539116606126093	30.13810968697389	24.82340323236806	19.499370474531958
6	26.970543353642164	34.00225439260842	24.163326081328577	14.863876172420834
7	83.86018906667825	4.706567164659099	7.575691794621939	3.8575519740407174
8	84.96825036943416	3.594325105282708	7.418109429696558	4.019315095586569
9	79.00102428537201	6.0159479785237755	9.368271594813931	5.614756141290278
10	43.81915333246502	26.11268425478817	16.1718098013015	13.896352611445304
11	33.22013130791755	24.017481994606822	23.463210145731527	19.2991765517441
12	29.652337927341666	22.761164630155797	27.256764383812754	20.329733058689783
13	26.796559558898014	23.70923159301299	29.643494019106058	19.850714828982934
14	22.108966597362585	24.97455366403118	30.7137677139462	22.20271202466003
15	20.788973093615184	24.447456733188936	32.59108823486848	22.172481938327408
16	26.00671815429243	24.716150745219867	28.51726250488425	20.75986859560345
17	25.276372132362756	24.82903117397254	26.655539422122953	23.23905727154175
18	26.745425689463048	22.865844343999086	29.14501919128087	21.243710775256996
19	25.02118518018257	25.884833018972596	26.25949313149927	22.834488669345568
20	26.63913799230419	26.569833911403336	26.753304807709316	20.037723288583155
21	25.30595902536915	26.613249460923594	28.531895153055892	19.548896360651362
22	25.53895580779454	23.11540335457479	28.68352797971368	22.662112857916984
23	23.70971398800766	25.935162896749787	28.653619490044168	21.701503625198384
24	23.90556635584349	23.832563913316836	29.964929883887525	22.29693984695215
25	24.014587624638807	25.77999250679775	27.279115351899108	22.926304516664338
26	22.861502789047062	26.282648091243406	27.23859417234687	23.617254947362667
27	23.248544373103584	25.23279578451094	28.362574509926887	23.15608533245859
28	20.25930338946803	26.57562265133937	28.456159138892772	24.708914820299825
29	22.456451791856207	26.433476926243415	27.19968097611019	23.910390305790187
30	24.65569057255462	24.514348839116444	28.29134084904735	22.538619739281586
31	25.2577195259022	25.236654944468295	27.0628415959556	22.442783933673905
32	25.104317917597285	25.360308861435254	26.947066797234914	22.588306423732547
33	25.204816874820107	24.524318335672948	26.146934299409708	24.123930490097234
34	21.760677411211184	25.05945518309302	30.29569205189927	22.88417535379653
35	22.828539131077978	26.37832309851953	27.910409601589976	22.882728168812523
36	24.815845710784906	26.746872874447053	25.120076154089826	23.317205260678215
37	22.601331088588626	26.547322144985426	27.34214829786926	23.50919846855669
38	22.26397618898306	26.803313088823387	27.192284252858595	23.740426469334956
39	22.940133173178197	25.32396843850348	26.4849323923415	25.250965995976827
40	25.387001384473635	22.638153906515065	28.34633387843968	23.62851083057162
41	20.955720963439283	23.38699173657374	26.91249515595026	28.744792144036712
42	21.97084083055554	23.96457934352473	27.451651961659245	26.612927864260485
43	22.08661562927623	23.708909996349878	28.286516899100654	25.917957475273234
44	22.476551583300772	24.383941392224116	27.697512610609152	25.44199441386596
45	22.76438059678693	23.828865551691038	27.426728220267986	25.98002563125405
46	23.031788222165407	23.912802280763536	27.480434863007865	25.574974634063196
47	20.820811163263368	24.55197564870067	29.605384814527163	25.021828373508797
48	21.499219324100295	24.678041540640976	29.366920888828858	24.455818246429875
49	24.341169036030085	22.987729479318922	28.58528019913265	24.085821285518342
50	21.61129576119518	23.903475977533255	29.31835979269879	25.166868468572773
51	21.454195791264468	23.14997499585944	27.25965875378077	28.136170459095318
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	526.5
2	795.0
3	2459.0
4	4123.0
5	2634.0
6	1145.0
7	1104.0
8	1063.0
9	1085.0
10	1107.0
11	1092.5
12	1078.0
13	1088.5
14	1099.0
15	1150.5
16	1202.0
17	1180.5
18	1159.0
19	1161.5
20	1164.0
21	1254.0
22	1344.0
23	1536.0
24	1728.0
25	1997.5
26	2966.0
27	3665.0
28	4770.5
29	5876.0
30	7176.0
31	8476.0
32	9739.5
33	11003.0
34	12710.0
35	14417.0
36	15716.0
37	17015.0
38	18268.0
39	19521.0
40	21390.0
41	23259.0
42	26282.5
43	29306.0
44	32228.0
45	35150.0
46	40033.0
47	44916.0
48	50527.5
49	56139.0
50	57755.0
51	59371.0
52	54211.0
53	49051.0
54	45052.5
55	41054.0
56	38766.5
57	36479.0
58	34930.5
59	33382.0
60	30161.0
61	26940.0
62	24465.5
63	21991.0
64	20287.0
65	18583.0
66	16738.0
67	14893.0
68	13556.0
69	12219.0
70	10226.5
71	8234.0
72	6378.5
73	4523.0
74	3854.0
75	2609.5
76	2034.0
77	1433.0
78	832.0
79	636.5
80	441.0
81	357.5
82	274.0
83	179.0
84	84.0
85	56.5
86	29.0
87	18.0
88	7.0
89	6.5
90	6.0
91	4.0
92	2.0
93	3.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	621897.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.94964841582607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.71055448620703	23.628375580244604
2	9.917961397383579	6.535866820910479
3	3.587380563459515	3.5460878489927703
4	1.988708929280472	2.6210904008482188
5	1.3472244825514597	2.21952865186319
6	1.0436509077381433	2.0632758287297728
7	0.8786659591739212	2.0266214100814697
8	0.7819709552042056	2.0612534436292997
9	0.7199948326817036	2.135121893826628
>10	7.922308133414457	48.27436509752387
>50	0.0626241641700587	1.3917687359376623
>100	0.03550346315131443	2.1284345599692513
>500	0.001972414619517468	0.5191105663436456
>1k	0.0014793109646381011	0.8490991610991838
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2607	0.4192012503678262	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1576	0.25341817053306254	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1043	0.16771265981344177	No Hit
GCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	944	0.1517936249893471	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	806	0.12960345523454847	TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCT	760	0.12220673198294893	TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG	685	0.11014685711621057	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.431933262260471E-4	0.0	0.0	0.12735227859275733	0.0
2	6.431933262260471E-4	0.0	0.0	0.5007260044669777	0.0
3	8.039916577825589E-4	0.0	0.0	0.7882334212900207	0.0
4	8.039916577825589E-4	0.0	0.0	1.1362010107783123	0.0
5	8.039916577825589E-4	0.0	0.0	2.1788173925907346	0.0
6	8.039916577825589E-4	0.0	0.0	3.0255814065673254	0.0
7	8.039916577825589E-4	0.0	0.0	3.6954672558317534	0.0
8	8.039916577825589E-4	0.0	0.0	4.723933384467203	0.0
9	8.039916577825589E-4	0.0	0.0	5.176580687798784	0.0
10	8.039916577825589E-4	0.0	0.0	6.104869455874526	0.0
11	8.039916577825589E-4	0.0	0.0	7.435636447836218	0.0
12	8.039916577825589E-4	0.0	0.0	8.411039127058018	0.0
13	8.039916577825589E-4	0.0	0.0	8.837154705682774	0.0
14	8.039916577825589E-4	0.0	0.0	9.060825184877881	0.0
15	8.039916577825589E-4	0.0	0.0	9.380974663006896	0.0
16	9.647899893390706E-4	0.0	0.0	10.003907399456823	0.0
17	9.647899893390706E-4	0.0	0.0	10.741810942969655	0.0
18	9.647899893390706E-4	0.0	0.0	11.483252049776732	0.0
19	9.647899893390706E-4	0.0	0.0	11.979958095954796	0.0
20	9.647899893390706E-4	0.0	0.0	12.471518595523053	0.0
21	9.647899893390706E-4	0.0	0.0	13.098953685256562	0.0
22	9.647899893390706E-4	0.0	0.0	13.76047802128005	0.0
23	9.647899893390706E-4	0.0	0.0	14.413158449067932	0.0
24	9.647899893390706E-4	0.0	0.0	14.927230715054101	0.0
25	9.647899893390706E-4	0.0	0.0	15.413484869680993	0.0
26	9.647899893390706E-4	0.0	0.0	15.885267174467797	0.0
27	9.647899893390706E-4	0.0	0.0	16.377792464025394	0.0
28	9.647899893390706E-4	0.0	0.0	16.83606770896145	0.0
29	9.647899893390706E-4	0.0	0.0	17.341617663375125	0.0
30	9.647899893390706E-4	0.0	0.0	18.079038811893287	0.0
31	9.647899893390706E-4	0.0	0.0	18.610155701024446	0.0
32	0.0011255883208955823	0.0	0.0	19.227942890864565	0.0
33	0.0011255883208955823	0.0	0.0	19.742497551845403	0.0
34	0.0011255883208955823	0.0	0.0	20.250781077895535	0.0
35	0.0011255883208955823	0.0	0.0	20.831102256482986	0.0
36	0.0012863866524520941	0.0	0.0	21.341958555838026	0.0
37	0.0012863866524520941	0.0	0.0	21.852814855193063	0.0
38	0.0012863866524520941	0.0	0.0	22.43088485713872	0.0
39	0.0012863866524520941	0.0	0.0	23.280221644420216	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCGC	20	7.0307864E-4	45.0	21
TCGTGAC	20	7.0307864E-4	45.0	18
CGTCGCA	20	7.0307864E-4	45.0	27
GCGATGT	105	0.0	42.85714	9
CGTTTTT	1185	0.0	41.58228	1
CGTAAGG	115	0.0	41.086956	2
ACTACGG	40	3.4548066E-7	39.375	2
ACAACGA	310	0.0	39.193546	13
CGTTAGG	75	0.0	39.000004	2
TTACACG	1040	0.0	38.942307	34
TACACGC	1050	0.0	38.785717	35
CAAGGGA	645	0.0	38.372093	4
ACACGCG	1050	0.0	38.142857	36
ATATGCG	30	1.1393193E-4	37.500004	1
GATATAC	30	1.1393193E-4	37.500004	9
CACAACG	340	0.0	37.058826	12
CACGGGA	305	0.0	36.885246	4
AACGGGA	240	0.0	36.562504	4
TATGCGG	80	0.0	36.5625	2
GTCTTAC	1145	0.0	36.55022	31
>>END_MODULE