FastQCFastQC Report
Sat 14 Jan 2017
SRR2934533.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934533.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343659
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC24530.713788959404526TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC19020.553455605702164TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT15040.4376431287991876TruSeq Adapter, Index 22 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC9100.26479737181333823TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC6240.18157534067200334TruSeq Adapter, Index 22 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG4320.12570600508061772No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.1248330467120023No Hit
CGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG3890.11319360179713028TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG3760.1094107821997969No Hit
AGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG3670.1067919070939507TruSeq Adapter, Index 22 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC3620.10533697647959168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG302.161045E-645.0000042
GACCGAT850.045.0000049
GTCTCGC600.045.00000431
CGCGCAG600.045.00000435
ACGGGCC600.045.0000045
GCGATCT302.161045E-645.0000049
CTCGCGC600.045.00000433
GGACCGA850.045.0000048
CGGTAGT302.161045E-645.00000412
CGCCGGT302.161045E-645.00000428
GTTGGCG302.161045E-645.0000041
GGTTACG302.161045E-645.0000041
TCGCGCA600.045.00000434
AGTAACG302.161045E-645.0000041
AGTCGCC302.161045E-645.00000413
AGCCGAG207.0263783E-445.01
GATACCG406.7902874E-945.01
ACACGAC502.1827873E-1145.026
CTCCGGG207.0263783E-445.03
ACCATCG207.0263783E-445.024
Produced by FastQC (version 0.11.5)