SRR2934532.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934532.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345973
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	963	0.27834542001832513	RNA PCR Primer, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	875	0.25290990915476064	RNA PCR Primer, Index 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	532	0.15376922476609448	Illumina Paired End PCR Primer 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	460	0.13295835224135988	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGGTT	25	3.885133E-5	45.000004	19
ACGCGAG	20	7.026444E-4	45.0	1
CCCTCGT	20	7.026444E-4	45.0	14
AGACGGT	20	7.026444E-4	45.0	10
GCCGATA	35	1.2086457E-7	45.0	9
CTAACGG	20	7.026444E-4	45.0	2
TCTACGG	20	7.026444E-4	45.0	2
TACGAGA	20	7.026444E-4	45.0	2
GCGGGTT	20	7.026444E-4	45.0	5
TGTTACG	20	7.026444E-4	45.0	1
ATCGCAT	20	7.026444E-4	45.0	10
TACGTTG	20	7.026444E-4	45.0	1
ACGAATT	20	7.026444E-4	45.0	27
TACAGCG	30	2.1610886E-6	44.999996	1
ATTGGGA	185	0.0	40.135136	4
AATGCGG	45	1.9215804E-8	40.0	2
TACGGGT	40	3.4488767E-7	39.375	4
AGTTGCG	40	3.4488767E-7	39.375	1
CGGTAGT	40	3.4488767E-7	39.375	12
CGTGCGT	35	6.2356357E-6	38.571426	12