##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934532.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345973
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.216019747205706	33.0	31.0	34.0	30.0	34.0
2	32.33977506915279	34.0	31.0	34.0	30.0	34.0
3	32.3949123197475	34.0	31.0	34.0	30.0	34.0
4	35.91915843143829	37.0	35.0	37.0	35.0	37.0
5	35.9420503912155	37.0	35.0	37.0	35.0	37.0
6	35.9667575215407	37.0	35.0	37.0	35.0	37.0
7	36.14284351669061	37.0	36.0	37.0	35.0	37.0
8	35.90700430380405	37.0	35.0	37.0	35.0	37.0
9	37.6242683677628	39.0	38.0	39.0	35.0	39.0
10	37.312434785373426	39.0	37.0	39.0	34.0	39.0
11	37.187927959696275	39.0	37.0	39.0	34.0	39.0
12	36.97419451806933	39.0	35.0	39.0	34.0	39.0
13	36.89158691574198	39.0	35.0	39.0	33.0	39.0
14	38.071895783775034	40.0	37.0	41.0	33.0	41.0
15	38.25551704901827	40.0	37.0	41.0	34.0	41.0
16	38.335786318585555	40.0	37.0	41.0	34.0	41.0
17	38.32903723700982	40.0	37.0	41.0	34.0	41.0
18	38.32988123350666	40.0	37.0	41.0	34.0	41.0
19	38.330774366785846	40.0	37.0	41.0	34.0	41.0
20	38.205686570917386	40.0	36.0	41.0	34.0	41.0
21	38.171614547956054	40.0	36.0	41.0	34.0	41.0
22	38.12906787523882	40.0	36.0	41.0	34.0	41.0
23	38.15337902090626	40.0	36.0	41.0	34.0	41.0
24	38.06297890297798	40.0	36.0	41.0	34.0	41.0
25	37.969549646937764	40.0	36.0	41.0	34.0	41.0
26	37.81312992632373	40.0	35.0	41.0	34.0	41.0
27	37.75145170287855	40.0	35.0	41.0	33.0	41.0
28	37.78010422778656	40.0	36.0	41.0	33.0	41.0
29	37.79898431380484	40.0	36.0	41.0	34.0	41.0
30	37.69436343298465	40.0	36.0	41.0	33.0	41.0
31	37.70755521384617	40.0	35.0	41.0	33.0	41.0
32	37.65980293259879	40.0	35.0	41.0	33.0	41.0
33	37.63645428978562	40.0	35.0	41.0	33.0	41.0
34	37.57430782170863	40.0	35.0	41.0	33.0	41.0
35	37.54956600659589	40.0	35.0	41.0	33.0	41.0
36	37.442664600994874	40.0	35.0	41.0	33.0	41.0
37	37.40805496382666	40.0	35.0	41.0	33.0	41.0
38	37.32684053379888	39.0	35.0	41.0	33.0	41.0
39	37.27384217843589	39.0	35.0	41.0	33.0	41.0
40	37.16790327568914	39.0	35.0	41.0	33.0	41.0
41	37.09444089567683	39.0	35.0	41.0	33.0	41.0
42	37.07075118578617	39.0	35.0	41.0	33.0	41.0
43	37.010359189878976	39.0	35.0	41.0	33.0	41.0
44	36.92873432319863	39.0	35.0	41.0	32.0	41.0
45	36.796559269075914	39.0	35.0	41.0	32.0	41.0
46	36.72932569882621	39.0	35.0	41.0	32.0	41.0
47	36.68794674728953	39.0	35.0	41.0	32.0	41.0
48	36.622245088489564	38.0	35.0	41.0	32.0	41.0
49	36.56923806192969	38.0	35.0	41.0	32.0	41.0
50	36.441485318218476	38.0	35.0	40.0	31.0	41.0
51	35.4480002774783	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	13.0
11	5.0
12	5.0
13	9.0
14	8.0
15	13.0
16	23.0
17	30.0
18	79.0
19	132.0
20	190.0
21	342.0
22	494.0
23	676.0
24	1029.0
25	1351.0
26	1785.0
27	2382.0
28	2892.0
29	3634.0
30	4363.0
31	5670.0
32	7674.0
33	11168.0
34	23320.0
35	33568.0
36	24207.0
37	36324.0
38	64902.0
39	119537.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.03262682348045	20.625597951285215	24.266633523425238	11.0751417018091
2	32.46293785931272	23.109317779133054	24.91379385096525	19.513950510588977
3	33.40896543949962	23.838565437187295	24.750775349521493	18.001693773791597
4	28.99792758394441	26.871750107667363	24.01170033499724	20.118621973390987
5	25.807794249840306	30.600364768348975	23.144002566674278	20.44783841513644
6	27.779624421558907	35.012269743592704	22.157509401022622	15.050596433825762
7	84.07592499992774	5.130169117243253	6.473337514777165	4.320568368051842
8	84.3938688857223	4.483008789703242	6.682602399609218	4.440519924965243
9	78.23269445881616	7.244784997673229	8.695187196688758	5.827333346821862
10	44.760429282053806	26.27083616351565	15.38848407245652	13.580250481974026
11	33.85177456044258	24.459134094279033	22.197107866798856	19.491983478479536
12	31.564312822098834	21.97223482757325	26.33471398057074	20.128738369757176
13	25.718191882025476	25.851150234266836	27.613715521153388	20.816942362554304
14	21.448783575596938	26.788506617568427	28.341228939830565	23.42148086700407
15	20.79815476930281	26.0977012657057	31.7371586800126	21.366985284978885
16	22.966243030525504	23.80677104860784	30.735057359967392	22.49192856089926
17	22.96479783104462	24.70163856717143	26.9049896957277	25.42857390605625
18	23.190537989958752	24.818988765019235	28.860344593364225	23.130128651657788
19	25.045596043621902	25.593904726669418	26.119379257918972	23.241119971789708
20	26.61826211872025	25.227113098420972	27.229581499134326	20.925043283724452
21	26.103193023733066	26.511317357134807	26.57028149595489	20.815208123177243
22	24.370687886048913	25.573671933937042	26.315348307526886	23.74029187248716
23	23.48189020530504	26.249158171302383	25.981507227442602	24.287444395949972
24	23.682483893251785	24.25911848612464	28.938963445124333	23.119434175499247
25	22.519965430828417	26.3789370846858	26.76567246577045	24.335425018715334
26	22.693100328638362	26.429230026620576	27.142002410592735	23.73566723414833
27	22.05229887881424	26.89487329936151	27.274671722937917	23.77815609888633
28	20.340026533862467	27.394912319747494	28.628534596630374	23.63652654975966
29	22.710153682512797	24.13454229087241	27.74898619256416	25.406317834050636
30	23.10324794131334	26.780991580267827	26.5451350249875	23.570625453431337
31	25.20167758755741	27.470640772545835	24.37271116532215	22.954970474574605
32	26.394834278975527	25.778312180430262	24.80366965052186	23.023183890072346
33	25.021605732239223	26.555251421353688	24.203911865954858	24.21923098045223
34	22.919418567344852	26.276327921543007	26.814231168328163	23.990022342783973
35	23.838854477083473	25.193584470464458	26.743994473557187	24.223566578894882
36	24.61810603717631	28.101903905796117	24.866102268096064	22.413887788931504
37	23.098334263078332	28.419269711798318	26.484147606894183	21.998248418229167
38	23.14573680605134	27.545213065759466	26.313614068149825	22.995436060039367
39	24.49526408130114	27.872984308024034	26.02255089269972	21.609200717975103
40	24.842400996609562	26.17863243663523	26.804114771961974	22.174851794793234
41	21.075344029736424	24.890092579478747	27.477288690157902	26.557274700626927
42	21.746494668659114	25.206880305688596	26.89227194029592	26.154353085356373
43	23.178109274423147	24.914371930757603	27.090842348969428	24.81667644584982
44	22.287577354302215	25.8728282264801	26.62086347778584	25.218730941431843
45	23.57380489228928	25.442158781176566	25.563266497674675	25.42076982885948
46	23.361360568599284	26.9862099065534	25.723105560260485	23.929323964586832
47	21.50861483410555	25.89826373734366	28.899364979348096	23.69375644920268
48	21.886679018304896	24.31345798660589	29.167015923207877	24.63284707188133
49	23.058157717509747	24.57214869368419	28.43892442473835	23.930769164067716
50	22.260118564165413	24.442658820196954	28.68056177794221	24.616660837695427
51	21.345018252869444	25.110919060157872	26.57172669543577	26.972335991536912
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	114.0
1	313.5
2	513.0
3	721.5
4	930.0
5	667.0
6	404.0
7	415.5
8	427.0
9	404.0
10	381.0
11	425.5
12	470.0
13	453.5
14	437.0
15	416.0
16	395.0
17	406.0
18	417.0
19	438.5
20	460.0
21	504.5
22	549.0
23	672.5
24	796.0
25	1201.0
26	1761.0
27	1916.0
28	2366.5
29	2817.0
30	3579.0
31	4341.0
32	5231.0
33	6121.0
34	7266.5
35	8412.0
36	8889.5
37	9367.0
38	10082.0
39	10797.0
40	12337.0
41	13877.0
42	15876.5
43	17876.0
44	20778.0
45	23680.0
46	25443.0
47	27206.0
48	30121.0
49	33036.0
50	33570.0
51	34104.0
52	30181.0
53	26258.0
54	24377.0
55	22496.0
56	20895.0
57	19294.0
58	18172.5
59	17051.0
60	16443.0
61	15835.0
62	14137.5
63	12440.0
64	11079.5
65	9719.0
66	7890.5
67	6062.0
68	5516.0
69	4970.0
70	4409.0
71	3848.0
72	3360.0
73	2872.0
74	2218.5
75	1361.5
76	1158.0
77	828.0
78	498.0
79	371.0
80	244.0
81	170.5
82	97.0
83	72.0
84	47.0
85	40.0
86	33.0
87	32.0
88	31.0
89	18.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	345973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.892751183456756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.23043398639682	20.694881374780884
2	10.965221996609978	6.5556130563205794
3	3.6132542137588186	3.2403032752340715
4	1.8743313771305503	2.2411568596763756
5	1.394765776833796	2.0846693163046717
6	1.0283309272455154	1.844378432544214
7	0.9364776440806675	1.9595725242361213
8	0.7967046215408006	1.9052554414743388
9	0.6998620547144031	1.8828722037888408
>10	9.359781357205057	54.16607574071871
>50	0.07487825085415632	1.487734647380164
>100	0.022962663595274605	1.23017898024093
>500	0.002995130034166253	0.707308147300098
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	963	0.27834542001832513	RNA PCR Primer, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	875	0.25290990915476064	RNA PCR Primer, Index 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	532	0.15376922476609448	Illumina Paired End PCR Primer 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	460	0.13295835224135988	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.671196885306079E-4	0.0	0.0	0.15897194289727812	0.0
2	8.671196885306079E-4	0.0	0.0	0.8838840025088663	0.0
3	8.671196885306079E-4	0.0	0.0	1.1937347712104702	0.0
4	8.671196885306079E-4	0.0	0.0	1.6151549398363456	0.0
5	8.671196885306079E-4	0.0	0.0	2.75772964942351	0.0
6	8.671196885306079E-4	0.0	0.0	3.3496833567937383	0.0
7	8.671196885306079E-4	0.0	0.0	3.951175380737803	0.0
8	8.671196885306079E-4	0.0	0.0	4.941715104935934	0.0
9	8.671196885306079E-4	0.0	0.0	5.272087706266096	0.0
10	8.671196885306079E-4	0.0	0.0	6.093539091200759	0.0
11	8.671196885306079E-4	0.0	0.0	7.397109022958439	0.0
12	8.671196885306079E-4	0.0	0.0	8.363658435773889	0.0
13	8.671196885306079E-4	0.0	0.0	8.743456859350296	0.0
14	8.671196885306079E-4	0.0	0.0	8.907631520378757	0.0
15	0.0011561595847074772	0.0	0.0	9.257947874545124	0.0
16	0.0011561595847074772	0.0	0.0	9.953666904642848	0.0
17	0.0011561595847074772	0.0	0.0	10.781188127397225	0.0
18	0.0011561595847074772	0.0	0.0	11.575469762091261	0.0
19	0.0011561595847074772	0.0	0.0	12.071173184034592	0.0
20	0.0011561595847074772	0.0	0.0	12.593468276426195	0.0
21	0.0011561595847074772	0.0	0.0	13.237738205004437	0.0
22	0.0014451994808843465	0.0	0.0	13.878250614932378	0.0
23	0.0014451994808843465	0.0	0.0	14.553447812401545	0.0
24	0.0014451994808843465	0.0	0.0	15.091929138979053	0.0
25	0.0014451994808843465	0.0	0.0	15.613068071785948	0.0
26	0.0014451994808843465	0.0	0.0	16.086226381827483	0.0
27	0.0014451994808843465	0.0	0.0	16.544932697060176	0.0
28	0.0014451994808843465	0.0	0.0	16.954791269838974	0.0
29	0.0014451994808843465	0.0	0.0	17.413497585071667	0.0
30	0.0014451994808843465	0.0	0.0	17.927121480577963	0.0
31	0.0014451994808843465	0.0	0.0	18.39478803259214	0.0
32	0.0014451994808843465	0.0	0.0	18.876328499622804	0.0
33	0.0014451994808843465	0.0	0.0	19.344573131429332	0.0
34	0.0014451994808843465	0.0	0.0	19.837097114514716	0.0
35	0.0014451994808843465	0.0	0.0	20.375000361299872	0.0
36	0.0014451994808843465	0.0	0.0	20.83312859674021	0.0
37	0.0014451994808843465	0.0	0.0	21.304263627508504	0.0
38	0.0014451994808843465	0.0	0.0	21.75892338419472	0.0
39	0.0017342393770612159	0.0	0.0	22.25202544707246	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGGTT	25	3.885133E-5	45.000004	19
ACGCGAG	20	7.026444E-4	45.0	1
CCCTCGT	20	7.026444E-4	45.0	14
AGACGGT	20	7.026444E-4	45.0	10
GCCGATA	35	1.2086457E-7	45.0	9
CTAACGG	20	7.026444E-4	45.0	2
TCTACGG	20	7.026444E-4	45.0	2
TACGAGA	20	7.026444E-4	45.0	2
GCGGGTT	20	7.026444E-4	45.0	5
TGTTACG	20	7.026444E-4	45.0	1
ATCGCAT	20	7.026444E-4	45.0	10
TACGTTG	20	7.026444E-4	45.0	1
ACGAATT	20	7.026444E-4	45.0	27
TACAGCG	30	2.1610886E-6	44.999996	1
ATTGGGA	185	0.0	40.135136	4
AATGCGG	45	1.9215804E-8	40.0	2
TACGGGT	40	3.4488767E-7	39.375	4
AGTTGCG	40	3.4488767E-7	39.375	1
CGGTAGT	40	3.4488767E-7	39.375	12
CGTGCGT	35	6.2356357E-6	38.571426	12
>>END_MODULE