##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934531.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	430183
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06184344802096	33.0	31.0	34.0	30.0	34.0
2	32.192945792837	33.0	31.0	34.0	30.0	34.0
3	32.2406766422662	34.0	31.0	34.0	30.0	34.0
4	35.80670319375707	37.0	35.0	37.0	35.0	37.0
5	35.85861830895224	37.0	35.0	37.0	35.0	37.0
6	35.8845956255826	37.0	35.0	37.0	35.0	37.0
7	36.1008268574072	37.0	35.0	37.0	35.0	37.0
8	35.89530269675929	37.0	35.0	37.0	35.0	37.0
9	37.64831478696276	39.0	38.0	39.0	35.0	39.0
10	37.07335947724573	39.0	37.0	39.0	33.0	39.0
11	37.10577823856359	39.0	37.0	39.0	34.0	39.0
12	37.003626363663834	39.0	37.0	39.0	34.0	39.0
13	36.982921221898586	39.0	37.0	39.0	33.0	39.0
14	38.11828919320382	40.0	37.0	41.0	33.0	41.0
15	38.27917653649726	40.0	37.0	41.0	34.0	41.0
16	38.372153246409084	40.0	37.0	41.0	34.0	41.0
17	38.390894107856425	40.0	37.0	41.0	34.0	41.0
18	38.37958961651204	40.0	37.0	41.0	34.0	41.0
19	38.35063449741157	40.0	37.0	41.0	34.0	41.0
20	38.2552843789736	40.0	37.0	41.0	34.0	41.0
21	38.239670093890275	40.0	37.0	41.0	34.0	41.0
22	38.25086997859051	40.0	37.0	41.0	34.0	41.0
23	38.25149064467912	40.0	37.0	41.0	34.0	41.0
24	38.17538117498832	40.0	37.0	41.0	34.0	41.0
25	38.10295153457947	40.0	36.0	41.0	34.0	41.0
26	37.95943586799106	40.0	36.0	41.0	34.0	41.0
27	37.91381807277368	40.0	36.0	41.0	34.0	41.0
28	37.947257330020015	40.0	36.0	41.0	34.0	41.0
29	37.94885200019527	40.0	36.0	41.0	34.0	41.0
30	37.8450357173575	40.0	36.0	41.0	34.0	41.0
31	37.88467233712164	40.0	36.0	41.0	34.0	41.0
32	37.832936215517584	40.0	36.0	41.0	34.0	41.0
33	37.79339722862131	40.0	36.0	41.0	34.0	41.0
34	37.7617688286148	40.0	36.0	41.0	33.0	41.0
35	37.75622002729071	40.0	36.0	41.0	33.0	41.0
36	37.648040485095876	40.0	36.0	41.0	33.0	41.0
37	37.61950611716409	40.0	35.0	41.0	33.0	41.0
38	37.571975182654825	40.0	35.0	41.0	33.0	41.0
39	37.50836969382797	40.0	35.0	41.0	33.0	41.0
40	37.46347949593545	40.0	35.0	41.0	33.0	41.0
41	37.38752112473064	40.0	35.0	41.0	33.0	41.0
42	37.36576759193181	40.0	35.0	41.0	33.0	41.0
43	37.31219969175909	39.0	35.0	41.0	33.0	41.0
44	37.225139068721916	39.0	35.0	41.0	33.0	41.0
45	37.11463493443488	39.0	35.0	41.0	32.0	41.0
46	37.04840265654384	39.0	35.0	41.0	32.0	41.0
47	37.00819651171711	39.0	35.0	41.0	32.0	41.0
48	36.978223221280246	39.0	35.0	41.0	32.0	41.0
49	36.9734671058596	39.0	35.0	41.0	32.0	41.0
50	36.85854392200529	39.0	35.0	41.0	32.0	41.0
51	35.90593538098902	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	4.0
10	5.0
11	4.0
12	5.0
13	3.0
14	1.0
15	8.0
16	15.0
17	30.0
18	64.0
19	99.0
20	183.0
21	267.0
22	460.0
23	710.0
24	1100.0
25	1588.0
26	2269.0
27	2698.0
28	3370.0
29	4060.0
30	5185.0
31	6876.0
32	9367.0
33	13538.0
34	26812.0
35	39699.0
36	29038.0
37	43861.0
38	83314.0
39	155437.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.867416890021225	21.2851274922533	23.518130656023136	10.329324961702344
2	33.8683769465553	22.907460313401508	24.758765455631675	18.465397284411516
3	32.16886766794597	24.31151393709189	25.771357770995134	17.74826062396701
4	29.319847599742438	26.050541281268668	25.47288014635632	19.156730972632577
5	24.310351641045788	30.19459160403828	24.761554966142317	20.733501788773616
6	25.44684471492365	34.562500145287004	25.580973678643744	14.409681461145604
7	81.63758214527306	4.928135235469556	8.527301171826874	4.906981447430512
8	81.82540918632303	3.655188605779401	8.559148083490049	5.960254124407519
9	75.93954200886598	6.025575162198414	11.117129221749813	6.917753607185779
10	40.00948433573618	21.49806012789906	21.383225278544245	17.10923025782051
11	30.934276807777156	24.940083638823477	23.400738755366902	20.724900798032465
12	27.694957727292802	22.099432102151876	29.41213390580288	20.793476264752442
13	23.11341917277066	27.068480158444196	31.162784210440677	18.655316458344473
14	20.80951595018864	29.08413396159309	29.320777436579316	20.785572651638955
15	19.216705448611403	26.27393458132934	34.95326407598627	19.556095894072985
16	21.034536464713856	25.074909980171228	32.484779733276305	21.405773821838615
17	21.230267118877315	25.169753337533095	27.164253352642948	26.435726190946646
18	21.62753990743474	25.062124723664112	32.121678448474256	21.188656920426887
19	22.06735273127948	28.052479991073564	28.045971133215396	21.834196144431555
20	22.82377499808221	27.84233686593845	28.928851209833955	20.405036926145385
21	21.1647136218772	28.51739840951409	30.260610019456834	20.05727794915187
22	20.626338093323074	25.86759588361232	27.226784879923194	26.279281143141407
23	19.127208653061604	27.5171264322393	28.671983783645565	24.68368113105353
24	21.648693695473785	25.079559164355636	30.31918974018034	22.952557399990237
25	18.933802590990346	27.79677486093128	28.2593686872796	25.010053860798777
26	18.64741284523098	29.77848961953401	28.83191571958911	22.7421818156459
27	20.499880283507252	28.635487687797983	28.732423178042833	22.13220885065193
28	17.18896376658306	27.598487155466394	31.51379761636327	23.69875146158728
29	19.812963320261375	27.15751203557556	29.504420211863327	23.52510443229974
30	21.54687656183531	29.231280641029517	27.26490819023532	21.956934606899857
31	21.417396782299626	28.655014261372486	26.697010342110218	23.23057861421767
32	24.718317553227347	28.42952882842883	25.095831309001053	21.756322309342767
33	24.13693707096747	27.64637375256577	25.27831178823896	22.938377388227803
34	19.863871887080613	26.140502995236908	28.672216242854788	25.32340887482769
35	22.194972837141403	27.518056269076187	26.42247601602109	23.864494877761324
36	22.701036535613913	30.587680126829746	26.2367411078541	20.47454222970224
37	20.77487952801482	28.20915749808802	29.280561993384215	21.735400980512946
38	23.822187301683236	27.741449569136854	26.133064300541864	22.303298828638045
39	23.95840839828631	26.09633574548506	27.14031005409326	22.80494580213537
40	24.370558576233837	24.46354225992194	26.969917453734805	24.195981710109418
41	20.381093627595696	27.20470125504727	25.89432869267265	26.51987642468438
42	21.21771432157942	27.34696629109007	27.720295781097814	23.715023606232695
43	21.993895621165876	26.558232194205722	29.00811980017806	22.43975238445034
44	21.74307213441721	25.34944430626036	27.512942166473337	25.394541392849092
45	22.771936594426094	24.778524488415396	25.87294244542439	26.57659647173412
46	24.345452981638047	25.466836206916593	26.558929571833385	23.62878123961198
47	19.609793971402866	26.506393790549605	29.76268239330703	24.121129844740494
48	20.96224165064635	24.865464232663772	29.45606869634551	24.716225420344365
49	22.745901162993423	22.98975087346548	30.725295978688138	23.539051984852957
50	21.557104767041004	22.567604949521485	29.887280529449097	25.98800975398842
51	20.57821903701448	22.853064858443965	26.615882078092344	29.95283402644921
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	132.0
1	249.0
2	366.0
3	756.5
4	1147.0
5	829.5
6	512.0
7	515.0
8	518.0
9	553.5
10	589.0
11	610.5
12	632.0
13	660.0
14	688.0
15	701.0
16	714.0
17	705.5
18	697.0
19	788.0
20	879.0
21	971.0
22	1063.0
23	1179.0
24	1295.0
25	1467.5
26	2041.0
27	2442.0
28	3084.5
29	3727.0
30	4479.5
31	5232.0
32	6048.0
33	6864.0
34	7862.0
35	8860.0
36	10449.5
37	12039.0
38	13997.0
39	15955.0
40	17648.0
41	19341.0
42	22712.5
43	26084.0
44	29346.5
45	32609.0
46	38648.5
47	44688.0
48	50691.0
49	56694.0
50	55700.5
51	54707.0
52	46549.0
53	38391.0
54	32137.0
55	25883.0
56	22195.0
57	18507.0
58	15877.5
59	13248.0
60	11736.5
61	10225.0
62	9203.0
63	8181.0
64	7034.0
65	5887.0
66	4586.5
67	3286.0
68	2942.5
69	2599.0
70	1947.0
71	1295.0
72	1221.0
73	1147.0
74	938.5
75	515.5
76	301.0
77	267.0
78	233.0
79	134.5
80	36.0
81	43.5
82	51.0
83	48.0
84	45.0
85	24.0
86	3.0
87	9.0
88	15.0
89	10.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	430183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.145106314441655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.24525814050871	25.390223235740855
2	12.9259297716161	9.344182116161411
3	4.902839200122152	5.316409323930818
4	2.3437611833644194	3.3886198859347414
5	1.3579098292301621	2.454089757147476
6	0.933536201380085	2.024565914835791
7	0.7263387877196011	1.8377514891699358
8	0.5413108135963289	1.5652589525276972
9	0.4939877466899672	1.6069715659926307
>10	5.384947466644086	40.15011252633016
>50	0.09287325610520701	2.144668790668088
>100	0.04676135971730702	2.8991527544604736
>500	0.002597853317628168	0.603070722769956
>1k	0.001948389988221126	1.274922964329946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2142	0.49792762614980135	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1830	0.42540035287307965	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	1459	0.3391579862523624	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	834	0.19387098048969856	No Hit
ACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	608	0.14133519920591933	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.14133519920591933	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	519	0.12064632958531601	No Hit
GCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	477	0.11088304279806502	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35659242694388205	0.0
2	0.0	0.0	0.0	1.6797502458256137	0.0
3	0.0	0.0	0.0	2.35062752363529	0.0
4	0.0	0.0	0.0	3.2386217028566913	0.0
5	0.0	0.0	0.0	5.583670205470695	0.0
6	0.0	0.0	0.0	6.819423361685608	0.0
7	0.0	0.0	0.0	7.967539396024483	0.0
8	0.0	0.0	0.0	9.86463900247104	0.0
9	0.0	0.0	0.0	10.444624729475596	0.0
10	0.0	0.0	0.0	12.092528063638033	0.0
11	0.0	0.0	0.0	14.650974120316237	0.0
12	0.0	0.0	0.0	16.35559750152842	0.0
13	0.0	0.0	0.0	17.080172856667975	0.0
14	0.0	0.0	0.0	17.344943895969855	0.0
15	0.0	0.0	0.0	17.877972862711914	0.0
16	0.0	0.0	0.0	19.15742835026024	0.0
17	0.0	0.0	0.0	20.68422043641892	0.0
18	0.0	0.0	0.0	22.254017476283348	0.0
19	0.0	0.0	0.0	23.174788404004808	0.0
20	0.0	0.0	0.0	24.024426813704864	0.0
21	0.0	0.0	0.0	25.202530086033153	0.0
22	0.0	0.0	0.0	26.378541225478457	0.0
23	0.0	0.0	0.0	27.654742284097697	0.0
24	0.0	0.0	0.0	28.618983083943345	0.0
25	0.0	0.0	0.0	29.504420211863323	0.0
26	0.0	0.0	0.0	30.311983504694513	0.0
27	0.0	0.0	0.0	31.067940853078806	0.0
28	0.0	0.0	0.0	31.885499891906466	0.0
29	0.0	0.0	0.0	32.639597566617	0.0
30	0.0	0.0	0.0	33.491560568409255	0.0
31	0.0	0.0	0.0	34.31795305718729	0.0
32	0.0	0.0	0.0	35.0890202541709	0.0
33	0.0	0.0	0.0	35.81592020140266	0.0
34	0.0	0.0	0.0	36.54444736309896	0.0
35	0.0	0.0	0.0	37.35642738090534	0.0
36	0.0	0.0	0.0	38.09309061492434	0.0
37	0.0	0.0	0.0	38.78233217026242	0.0
38	0.0	0.0	0.0	39.492030136011884	0.0
39	0.0	0.0	0.0	40.20335531622589	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.028358E-4	45.000004	1
TATCACG	20	7.028358E-4	45.000004	1
AACCGAT	20	7.028358E-4	45.000004	35
ACCGGTT	20	7.028358E-4	45.000004	36
ACGCCCC	20	7.028358E-4	45.000004	29
GGCGTAA	20	7.028358E-4	45.000004	25
TAACGTG	20	7.028358E-4	45.000004	29
CCGTCAG	20	7.028358E-4	45.000004	44
TACGAAT	40	6.7975634E-9	45.000004	12
ATGACGG	20	7.028358E-4	45.000004	2
TCCGTCA	20	7.028358E-4	45.000004	43
CGTAACG	20	7.028358E-4	45.000004	27
CCGGTTA	20	7.028358E-4	45.000004	37
CGACTGG	40	6.7975634E-9	45.000004	2
GCTAACG	20	7.028358E-4	45.000004	1
GTAACGT	20	7.028358E-4	45.000004	28
GCGTAAC	20	7.028358E-4	45.000004	26
GCCCGAT	20	7.028358E-4	45.000004	37
AATTTCG	35	1.2095552E-7	45.0	1
GCGACAC	25	3.886722E-5	45.0	15
>>END_MODULE