SRR2934529.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934529.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507229
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC	3891	0.7671091361101199	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG	3235	0.6377789913431605	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	3052	0.6017006125438411	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	2650	0.5224464689518935	TruSeq Adapter, Index 21 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1578	0.3111020860400332	No Hit
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	1395	0.27502370724071373	TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT	988	0.19478381559413993	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	930	0.18334913816047585	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTACGGACT	829	0.16343702745702632	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	742	0.14628501130653018	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT	728	0.1435249167535768	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTACGGACTCGTA	549	0.10823513639795831	No Hit
TCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	543	0.10705223873240687	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTC	512	0.10094060079372433	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGC	25	3.8877122E-5	45.000004	12
AATGCGA	25	3.8877122E-5	45.000004	25
TTGACGA	25	3.8877122E-5	45.000004	20
GCGATAA	25	3.8877122E-5	45.000004	9
TAATGCG	25	3.8877122E-5	45.000004	24
AGTACGG	55	1.8189894E-12	45.000004	2
TTTACGT	20	7.0295547E-4	45.0	28
TAGACCC	20	7.0295547E-4	45.0	31
AAGACCG	20	7.0295547E-4	45.0	24
TACGGGT	35	1.2101373E-7	45.0	4
ACGGGTC	20	7.0295547E-4	45.0	5
CGTTAGG	35	1.2101373E-7	45.0	2
GTTACGT	20	7.0295547E-4	45.0	44
TTACGTC	20	7.0295547E-4	45.0	45
ATGCGAG	20	7.0295547E-4	45.0	1
ATCGGAC	20	7.0295547E-4	45.0	1
TATCGGG	45	3.8380676E-10	45.0	3
TATCGAG	20	7.0295547E-4	45.0	1
CGCCCTA	20	7.0295547E-4	45.0	45
CGACCCC	75	0.0	42.000004	34