##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934529.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507229
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13327905147379	33.0	31.0	34.0	30.0	34.0
2	32.25687214256283	33.0	31.0	34.0	30.0	34.0
3	32.31415199052105	34.0	31.0	34.0	30.0	34.0
4	35.88069491294859	37.0	35.0	37.0	35.0	37.0
5	35.8993846960643	37.0	35.0	37.0	35.0	37.0
6	35.90916923125452	37.0	35.0	37.0	35.0	37.0
7	36.10029395006989	37.0	35.0	37.0	35.0	37.0
8	35.76458167809806	37.0	35.0	37.0	35.0	37.0
9	37.58157558026059	39.0	37.0	39.0	35.0	39.0
10	37.27122660573429	39.0	37.0	39.0	34.0	39.0
11	37.149356602244744	39.0	37.0	39.0	34.0	39.0
12	36.88077771578518	39.0	35.0	39.0	33.0	39.0
13	36.69712496722388	39.0	35.0	39.0	33.0	39.0
14	37.6219123906559	40.0	36.0	41.0	33.0	41.0
15	37.82261463757001	40.0	36.0	41.0	33.0	41.0
16	38.08853200428209	40.0	36.0	41.0	33.0	41.0
17	38.072558942804925	40.0	36.0	41.0	34.0	41.0
18	38.09347651652409	40.0	36.0	41.0	34.0	41.0
19	38.07988107935469	40.0	36.0	41.0	34.0	41.0
20	37.897030729709854	40.0	36.0	41.0	33.0	41.0
21	37.908502865569595	40.0	35.0	41.0	33.0	41.0
22	37.92773481011535	40.0	35.0	41.0	34.0	41.0
23	37.91565939644618	40.0	35.0	41.0	34.0	41.0
24	37.84388313759663	40.0	35.0	41.0	34.0	41.0
25	37.79296727907907	40.0	35.0	41.0	34.0	41.0
26	37.62698307864889	40.0	35.0	41.0	33.0	41.0
27	37.55626551321001	39.0	35.0	41.0	33.0	41.0
28	37.57542648389583	39.0	35.0	41.0	33.0	41.0
29	37.5888208284621	39.0	35.0	41.0	33.0	41.0
30	37.501008420259886	39.0	35.0	41.0	33.0	41.0
31	37.46666101504449	39.0	35.0	41.0	33.0	41.0
32	37.36293469024839	39.0	35.0	41.0	33.0	41.0
33	37.34104122595514	39.0	35.0	41.0	33.0	41.0
34	37.20968635468398	39.0	35.0	41.0	33.0	41.0
35	37.17220032766265	39.0	35.0	41.0	33.0	41.0
36	37.1221578419215	39.0	35.0	41.0	33.0	41.0
37	37.043611465432775	39.0	35.0	41.0	32.0	41.0
38	36.93574302731113	39.0	35.0	41.0	32.0	41.0
39	36.87740054295003	39.0	35.0	41.0	32.0	41.0
40	36.712559810263215	39.0	35.0	41.0	31.0	41.0
41	36.62910637995856	39.0	35.0	41.0	31.0	41.0
42	36.634888778047	39.0	35.0	41.0	31.0	41.0
43	36.5930201151748	39.0	35.0	41.0	32.0	41.0
44	36.55538031145696	38.0	35.0	41.0	31.0	41.0
45	36.41836133186391	38.0	35.0	41.0	31.0	41.0
46	36.33086436303918	38.0	35.0	40.0	31.0	41.0
47	36.23184399945587	38.0	35.0	40.0	31.0	41.0
48	36.18615852011616	38.0	35.0	40.0	31.0	41.0
49	36.1770896380136	38.0	35.0	40.0	31.0	41.0
50	36.03826476798448	37.0	35.0	40.0	31.0	41.0
51	35.059135025797026	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	7.0
10	6.0
11	5.0
12	9.0
13	12.0
14	13.0
15	24.0
16	26.0
17	60.0
18	111.0
19	215.0
20	406.0
21	790.0
22	1184.0
23	1536.0
24	2069.0
25	2496.0
26	3113.0
27	3687.0
28	4326.0
29	5448.0
30	7117.0
31	9383.0
32	12803.0
33	19637.0
34	40111.0
35	47937.0
36	37443.0
37	54741.0
38	96095.0
39	156297.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.69713679620054	19.551918364289108	21.925402530218108	9.825542309292253
2	32.82817031360589	25.304349711865843	22.605962987132045	19.261516987396227
3	32.660790293930354	26.81254423544395	23.95091763286405	16.575747837761643
4	28.398021406504753	24.864114630669775	27.320007333965528	19.41785662885994
5	28.046109351003196	28.106437131946322	23.5826421596557	20.264811357394784
6	26.829301952372596	35.184896762606236	23.391209887447285	14.594591397573877
7	80.289573348527	4.70044102367964	10.651402029458094	4.358583598335269
8	79.5891007809096	6.770117639172839	8.249922618777711	5.39085896113984
9	74.30923705072068	5.877029901681489	10.097411622758163	9.716321424839668
10	46.96576891305505	22.49181336240633	16.507731221992433	14.034686502546187
11	36.30983244254568	23.413487793481842	21.766302794201437	18.510376969771052
12	30.874614818947656	21.176431158313108	27.032168902014668	20.916785120724565
13	26.714955178035954	25.32721906673317	30.697771617947712	17.26005413728316
14	22.276328837665037	27.623814884401327	29.285194655668345	20.814661622265287
15	19.692880336100657	23.190708733136315	36.21125763708306	20.905153293679977
16	24.833556440976366	23.576136222495165	29.09238233618346	22.497925000345013
17	23.95722642041366	23.870086292384702	26.846453968523093	25.326233318678547
18	25.854199976736346	21.983955964662904	29.44666018701612	22.715183871584628
19	24.442608762511608	25.11350888849021	25.904670277133206	24.539212071864977
20	26.837385086420536	26.7707485179278	27.388418248956587	19.00344814669508
21	24.62162060923173	27.18988859075487	29.05669825660599	19.131792543407418
22	23.60708871141043	22.02673743023368	29.418270643042888	24.947903215313005
23	22.80764703910857	26.242190411037225	28.349522602217142	22.600639947637063
24	24.189271512472672	24.186117118697865	28.373377705139095	23.251233663690364
25	22.12905807830388	27.1792425117649	26.71988391830909	23.971815491622127
26	21.129706700523826	24.77086286470214	28.693154374059844	25.406276060714195
27	23.462183747380376	25.016314130304064	28.370223311364295	23.15127881095127
28	18.096362786828042	26.755370848275632	29.74376465068046	25.404501714215865
29	21.235378891979757	24.328656287396814	29.676930932576806	24.759033888046623
30	24.21490096189295	24.257879577074654	29.711432114488723	21.815787346543672
31	25.566164395174567	26.017045555360596	25.528311669876917	22.888478379587916
32	25.762722557267033	25.29449223131958	24.99620486998969	23.946580341423697
33	25.805701172448735	23.08779663623334	24.974124113566063	26.13237807775186
34	22.779257495135333	24.43669427418385	29.413933351602527	23.370114879078287
35	21.799226779225954	25.77908597497383	28.709714941377563	23.71197230442266
36	24.840850976580597	26.693465870445106	25.71757529636515	22.748107856609145
37	21.489899039684246	26.46654666827015	28.04315210683932	24.000402185206287
38	22.675951099010504	27.83239917276023	23.781960416301118	25.70968931192814
39	24.785254786299678	23.364397540361452	25.064418635369822	26.78592903796904
40	26.923342316783938	22.595711207363934	27.56644434762208	22.914502128230048
41	21.81953318915125	23.433202754574364	26.25224504119441	28.495019015079976
42	21.8784809228179	23.479532913141796	29.061429847268194	25.580556316772107
43	23.101597108998106	22.27100579817006	27.817810101551764	26.80958699128007
44	23.181639851033754	22.470126905204552	27.993864704108006	26.354368539653688
45	22.885323985813113	22.202397733568073	25.557489812293856	29.354788468324962
46	25.06086994237317	23.18301989831023	27.00515940531791	24.750950753998687
47	19.79756677950196	24.340288114441407	31.87810633855714	23.984038767499495
48	22.00367092575543	24.882449544485823	27.8868124653756	25.227067064383146
49	23.765596998594322	21.450666267110122	30.564301331351324	24.21943540294423
50	22.9953334687094	22.65505324025243	28.109985824942974	26.239627466095193
51	21.660236303523657	22.187414363137755	25.334119303115553	30.818230030223038
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	123.0
1	414.0
2	705.0
3	1017.5
4	1330.0
5	941.5
6	553.0
7	569.5
8	586.0
9	583.5
10	581.0
11	620.0
12	659.0
13	671.0
14	683.0
15	683.0
16	683.0
17	678.5
18	674.0
19	732.5
20	791.0
21	943.0
22	1095.0
23	1164.0
24	1233.0
25	1467.5
26	2268.0
27	2834.0
28	3037.5
29	3241.0
30	3839.5
31	4438.0
32	5621.0
33	6804.0
34	7934.5
35	9065.0
36	9793.5
37	10522.0
38	12223.0
39	13924.0
40	15987.0
41	18050.0
42	20900.0
43	23750.0
44	26694.5
45	29639.0
46	41154.5
47	52670.0
48	52538.5
49	52407.0
50	53965.5
51	55524.0
52	49416.0
53	43308.0
54	39034.0
55	34760.0
56	31884.0
57	29008.0
58	27765.0
59	26522.0
60	22726.0
61	18930.0
62	17458.5
63	15987.0
64	14225.0
65	12463.0
66	10947.5
67	9432.0
68	8470.5
69	7509.0
70	6699.0
71	5889.0
72	4816.0
73	3743.0
74	2916.5
75	1823.0
76	1556.0
77	1210.0
78	864.0
79	746.0
80	628.0
81	410.0
82	192.0
83	124.0
84	56.0
85	36.5
86	17.0
87	10.5
88	4.0
89	3.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	507229.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.95514559879035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.76004680433898	20.896723590024035
2	12.087944522701564	7.241922763352513
3	4.523139631175757	4.06473918646586
4	2.3103541436381927	2.7682797902980267
5	1.3812503970037255	2.068777837531679
6	0.9082045258764015	1.6323239283664783
7	0.7387918574153658	1.5491432359254629
8	0.5946811652455547	1.4251008711831115
9	0.4881892718887729	1.3161402647276077
>10	6.986109074809129	46.581021459552026
>50	0.1546354113564036	3.006689197331953
>100	0.05732174731314961	3.1320760633871005
>500	0.0053322555640139165	1.1622244383869644
>1k	0.003999191673010437	3.154837373467175
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC	3891	0.7671091361101199	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG	3235	0.6377789913431605	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	3052	0.6017006125438411	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	2650	0.5224464689518935	TruSeq Adapter, Index 21 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1578	0.3111020860400332	No Hit
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	1395	0.27502370724071373	TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT	988	0.19478381559413993	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	930	0.18334913816047585	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTACGGACT	829	0.16343702745702632	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	742	0.14628501130653018	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT	728	0.1435249167535768	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTACGGACTCGTA	549	0.10823513639795831	No Hit
TCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	543	0.10705223873240687	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTC	512	0.10094060079372433	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9429922185048566E-4	0.0	0.0	0.20424699691855158	0.0
2	3.9429922185048566E-4	0.0	0.0	1.255054423150096	0.0
3	3.9429922185048566E-4	0.0	0.0	1.7037669376159486	0.0
4	3.9429922185048566E-4	0.0	0.0	2.3780186069802793	0.0
5	3.9429922185048566E-4	0.0	0.0	4.652927967446656	0.0
6	3.9429922185048566E-4	0.0	0.0	5.507965830029434	0.0
7	3.9429922185048566E-4	0.0	0.0	6.460592750020208	0.0
8	3.9429922185048566E-4	0.0	0.0	7.828219601008618	0.0
9	3.9429922185048566E-4	0.0	0.0	8.28935254096276	0.0
10	3.9429922185048566E-4	0.0	0.0	9.982079100366896	0.0
11	3.9429922185048566E-4	0.0	0.0	11.726064558611593	0.0
12	3.9429922185048566E-4	0.0	0.0	13.770703173517287	0.0
13	3.9429922185048566E-4	0.0	0.0	14.324496430606294	0.0
14	3.9429922185048566E-4	0.0	0.0	14.583945318583913	0.0
15	3.9429922185048566E-4	0.0	0.0	15.11624926808207	0.0
16	3.9429922185048566E-4	0.0	0.0	16.039895195266833	0.0
17	3.9429922185048566E-4	0.0	0.0	16.995282209810558	0.0
18	3.9429922185048566E-4	0.0	0.0	17.98871909926286	0.0
19	3.9429922185048566E-4	0.0	0.0	18.821084756589233	0.0
20	3.9429922185048566E-4	0.0	0.0	19.491787732956908	0.0
21	3.9429922185048566E-4	0.0	0.0	20.28748356265119	0.0
22	3.9429922185048566E-4	0.0	0.0	21.11965207036664	0.0
23	3.9429922185048566E-4	0.0	0.0	21.944131743256005	0.0
24	3.9429922185048566E-4	0.0	0.0	22.608525932074073	0.0
25	3.9429922185048566E-4	0.0	0.0	23.201157662515353	0.0
26	3.9429922185048566E-4	0.0	0.0	23.768948541980052	0.0
27	3.9429922185048566E-4	0.0	0.0	24.38287243040126	0.0
28	3.9429922185048566E-4	0.0	0.0	24.91911937211792	0.0
29	3.9429922185048566E-4	0.0	0.0	25.50051357473646	0.0
30	3.9429922185048566E-4	0.0	0.0	26.33761082272504	0.0
31	3.9429922185048566E-4	0.0	0.0	26.923933765616713	0.0
32	3.9429922185048566E-4	0.0	0.0	27.448548880288786	0.0
33	3.9429922185048566E-4	0.0	0.0	28.018705555084587	0.0
34	3.9429922185048566E-4	0.0	0.0	28.61370308085697	0.0
35	3.9429922185048566E-4	0.0	0.0	29.290320545552404	0.0
36	5.914488327757285E-4	0.0	0.0	29.83011618026572	0.0
37	5.914488327757285E-4	0.0	0.0	30.378192098637893	0.0
38	5.914488327757285E-4	0.0	0.0	31.014985341926426	0.0
39	7.885984437009713E-4	0.0	0.0	31.98752437262065	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGC	25	3.8877122E-5	45.000004	12
AATGCGA	25	3.8877122E-5	45.000004	25
TTGACGA	25	3.8877122E-5	45.000004	20
GCGATAA	25	3.8877122E-5	45.000004	9
TAATGCG	25	3.8877122E-5	45.000004	24
AGTACGG	55	1.8189894E-12	45.000004	2
TTTACGT	20	7.0295547E-4	45.0	28
TAGACCC	20	7.0295547E-4	45.0	31
AAGACCG	20	7.0295547E-4	45.0	24
TACGGGT	35	1.2101373E-7	45.0	4
ACGGGTC	20	7.0295547E-4	45.0	5
CGTTAGG	35	1.2101373E-7	45.0	2
GTTACGT	20	7.0295547E-4	45.0	44
TTACGTC	20	7.0295547E-4	45.0	45
ATGCGAG	20	7.0295547E-4	45.0	1
ATCGGAC	20	7.0295547E-4	45.0	1
TATCGGG	45	3.8380676E-10	45.0	3
TATCGAG	20	7.0295547E-4	45.0	1
CGCCCTA	20	7.0295547E-4	45.0	45
CGACCCC	75	0.0	42.000004	34
>>END_MODULE