SRR2934525.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934525.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	247379
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	973	0.3933236046713747	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	972	0.3929193666398522	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	565	0.22839448781020216	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	356	0.14390873922200348	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	317	0.1281434559926267	TruSeq Adapter, Index 14 (95% over 21bp)
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG	252	0.10186798394366539	No Hit
ACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	251	0.10146374591214291	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGGC	55	1.8189894E-12	45.000004	4
CCCCTGA	20	7.0225424E-4	45.0	16
TCCGCAA	20	7.0225424E-4	45.0	32
CGCGGTC	20	7.0225424E-4	45.0	21
AGGAGAT	20	7.0225424E-4	45.0	19
CAATTTG	20	7.0225424E-4	45.0	10
CGATTTG	20	7.0225424E-4	45.0	10
ATAGAGG	40	6.7775545E-9	45.0	2
CTAAGCG	20	7.0225424E-4	45.0	1
CGATTAG	25	3.8819016E-5	45.0	10
GCGTTAG	25	3.8819016E-5	45.0	1
CGCTCAC	20	7.0225424E-4	45.0	28
TGCGCAC	20	7.0225424E-4	45.0	26
GCTTCGT	20	7.0225424E-4	45.0	16
TGCGATG	20	7.0225424E-4	45.0	1
AGTTCCG	20	7.0225424E-4	45.0	24
CTCGAGG	20	7.0225424E-4	45.0	2
GGTTGAC	25	3.8819016E-5	45.0	10
TAGTCCG	20	7.0225424E-4	45.0	1
TGCACGG	20	7.0225424E-4	45.0	2