SRR2934523.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934523.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	703330
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	1766	0.2510912373992294	Illumina PCR Primer Index 8 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1496	0.21270242986933585	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1374	0.19535637609656917	No Hit
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	1174	0.16692022237072215	RNA PCR Primer, Index 40 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	1169	0.16620931852757598	RNA PCR Primer, Index 40 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	1109	0.15767847240982186	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG	1052	0.14957416859795544	No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	998	0.14189640709197673	RNA PCR Primer, Index 40 (96% over 26bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	967	0.13748880326447047	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	55	1.8189894E-12	45.000004	1
TTCACGG	40	6.8084773E-9	45.0	2
CGCTAAT	20	7.031417E-4	45.0	15
GCGATAT	20	7.031417E-4	45.0	9
GTTCACG	35	1.2110286E-7	45.0	1
TATAGCG	40	6.8084773E-9	45.0	1
TTACGCG	35	1.2110286E-7	45.0	1
TACGTAG	40	6.8084773E-9	45.0	1
GCTATCG	25	3.8892573E-5	44.999996	1
GTCCGAC	25	3.8892573E-5	44.999996	26
ACATACG	105	0.0	40.714287	17
GCGTAAG	45	1.9264917E-8	40.0	1
ACGGGTA	85	0.0	39.705883	5
TAGTAGG	120	0.0	39.375004	2
GTTTACG	40	3.4556615E-7	39.375	1
GTTACGG	40	3.4556615E-7	39.375	2
CGTAAGG	80	0.0	39.375	2
GCTACGA	40	3.4556615E-7	39.375	10
TACGGTT	75	0.0	39.000004	33
ACGGGTG	75	0.0	39.000004	5