##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934523.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	703330
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19703979639714	33.0	31.0	34.0	30.0	34.0
2	32.317350319195825	34.0	31.0	34.0	30.0	34.0
3	32.37805866378514	34.0	31.0	34.0	30.0	34.0
4	35.911806691026975	37.0	35.0	37.0	35.0	37.0
5	35.930224787795204	37.0	35.0	37.0	35.0	37.0
6	35.9479206062588	37.0	35.0	37.0	35.0	37.0
7	36.13102100009953	37.0	35.0	37.0	35.0	37.0
8	35.899357342925796	37.0	35.0	37.0	35.0	37.0
9	37.64885473390869	39.0	38.0	39.0	35.0	39.0
10	37.23067692263945	39.0	37.0	39.0	34.0	39.0
11	37.176817425675004	39.0	37.0	39.0	34.0	39.0
12	36.96394153526794	39.0	35.0	39.0	33.0	39.0
13	36.89698576770506	39.0	35.0	39.0	33.0	39.0
14	37.954010208579184	40.0	37.0	41.0	33.0	41.0
15	38.144131488774825	40.0	37.0	41.0	33.0	41.0
16	38.29161844368931	40.0	37.0	41.0	34.0	41.0
17	38.29248574637794	40.0	37.0	41.0	34.0	41.0
18	38.28793880539718	40.0	37.0	41.0	34.0	41.0
19	38.25603201910909	40.0	37.0	41.0	34.0	41.0
20	38.121767875677136	40.0	36.0	41.0	34.0	41.0
21	38.11881620292039	40.0	36.0	41.0	34.0	41.0
22	38.098050701662096	40.0	36.0	41.0	34.0	41.0
23	38.10289622225698	40.0	36.0	41.0	34.0	41.0
24	38.02337594017033	40.0	36.0	41.0	34.0	41.0
25	37.93921061237257	40.0	36.0	41.0	34.0	41.0
26	37.776754866136805	40.0	35.0	41.0	34.0	41.0
27	37.7581789487154	40.0	35.0	41.0	33.0	41.0
28	37.76812733709638	40.0	35.0	41.0	33.0	41.0
29	37.749985071019296	40.0	35.0	41.0	33.0	41.0
30	37.63315513343665	40.0	35.0	41.0	33.0	41.0
31	37.634220067393684	40.0	35.0	41.0	33.0	41.0
32	37.54630116730411	40.0	35.0	41.0	33.0	41.0
33	37.517764065232534	40.0	35.0	41.0	33.0	41.0
34	37.46300598581036	40.0	35.0	41.0	33.0	41.0
35	37.41555457608804	40.0	35.0	41.0	33.0	41.0
36	37.33107929421467	40.0	35.0	41.0	33.0	41.0
37	37.3084953009256	40.0	35.0	41.0	33.0	41.0
38	37.20150711614747	39.0	35.0	41.0	33.0	41.0
39	37.11582329774075	39.0	35.0	41.0	32.0	41.0
40	37.0018256010692	39.0	35.0	41.0	32.0	41.0
41	36.921253181294695	39.0	35.0	41.0	32.0	41.0
42	36.895619410518535	39.0	35.0	41.0	32.0	41.0
43	36.84560021611477	39.0	35.0	41.0	32.0	41.0
44	36.73877696102825	39.0	35.0	41.0	32.0	41.0
45	36.593963004564	39.0	35.0	41.0	32.0	41.0
46	36.52680534030967	38.0	35.0	41.0	31.0	41.0
47	36.494582912715224	38.0	35.0	41.0	31.0	41.0
48	36.43361153370395	38.0	35.0	41.0	31.0	41.0
49	36.39056914961682	38.0	35.0	41.0	31.0	41.0
50	36.254796468229706	38.0	35.0	40.0	31.0	41.0
51	35.24144853767079	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	14.0
10	22.0
11	8.0
12	14.0
13	17.0
14	28.0
15	30.0
16	38.0
17	94.0
18	153.0
19	275.0
20	409.0
21	797.0
22	1154.0
23	1713.0
24	2475.0
25	3307.0
26	4251.0
27	5230.0
28	6185.0
29	7341.0
30	9266.0
31	12284.0
32	16202.0
33	24441.0
34	50067.0
35	60703.0
36	50322.0
37	76716.0
38	135419.0
39	234152.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.649936729557965	19.770093697126526	23.276555813060725	10.303413760254788
2	33.50162796980081	23.170631140431947	24.83158687955867	18.496154010208578
3	33.2866506476334	24.875236375527845	24.474002246456145	17.36411073038261
4	28.749662320674506	26.12401006639842	25.340594031251335	19.785733581675743
5	26.171782804657838	29.84744003526083	24.354286039270328	19.626491120811
6	26.929748482220294	35.273769069995595	23.34892582429301	14.447556623491106
7	82.97797619893933	5.169834928127622	7.803733666984204	4.0484552059488434
8	83.67651031521477	4.558599803790539	7.3137787382878585	4.4511111427068375
9	77.74316465954814	6.386333584519358	9.326489699003313	6.54401205692918
10	44.46248560419718	24.007507144583624	16.98989094735046	14.54011630386874
11	34.82575746804487	23.97836008701463	22.168398902364466	19.027483542576032
12	30.03881534983578	21.921146545718226	28.041033369826394	19.999004734619596
13	26.4147697382452	24.519784453954756	29.846586950649055	19.21885885715098
14	22.363470916923777	26.12201953563761	30.03767790368675	21.47683164375187
15	20.69910283934995	24.344617747003543	34.08712837501599	20.869151038630516
16	25.808368760041517	23.631723373096555	29.049805923250823	21.510101943611108
17	25.365760027298705	23.74561016876857	26.58296958753359	24.30566021639913
18	26.27685439267485	23.39797818947009	28.52729159853838	21.79787581931668
19	25.718083971961953	25.321257446717755	26.205053104517084	22.755605476803208
20	26.683917933260346	26.89733126697283	26.422589680519813	19.99616111924701
21	25.43073663856227	26.82851577495628	27.49918246058038	20.24156512590107
22	24.32002047403068	23.76139223408642	28.29838056104531	23.620206730837587
23	22.914563576130693	26.007564016891077	28.682837359418766	22.395035047559468
24	23.948217764065234	24.50627728093498	29.10355025379267	22.441954701207116
25	22.953521106735103	26.625623818122364	26.730553225370734	23.6903018497718
26	21.85162014985853	26.38206816146048	27.0834458931085	24.68286579557249
27	23.34778837814397	24.949739098289566	28.24648458049564	23.45598794307082
28	20.275404148834827	26.907994824620022	28.105014715709554	24.711586310835596
29	22.561386546855672	26.92747358992223	26.73723572149631	23.77390414172579
30	24.093953051910198	25.93732671718823	26.496808041744274	23.471912189157294
31	24.55689363456699	27.05543628168854	25.005900501898115	23.38176958184636
32	25.682112237498757	26.589367722121903	24.85547324868838	22.873046791690957
33	24.280067678045867	25.894388125062207	24.301110431802993	25.524433765088933
34	21.61574225470263	26.335148507812832	27.68003639827677	24.36907283920777
35	22.111100052606886	26.70979483315087	27.280792799965877	23.89831231427637
36	22.36460836307281	29.039853269446773	24.530021469296063	24.065516898184352
37	21.824747984587606	28.02766837757525	25.619837060839153	24.527746576997995
38	22.82811766880412	27.598140275546328	25.65538225299646	23.918359802653093
39	23.322906743633855	25.97486244010635	24.994952582713662	25.707278233546134
40	24.26826667424964	24.472580438769853	27.11628965066185	24.142863236318657
41	20.185830264598412	24.80428817198186	26.162683235465572	28.84719832795416
42	21.569675685666756	24.498172977123115	28.12733709638434	25.804814240825785
43	21.902663045796427	24.82462002189584	27.39524831871241	25.877468613595322
44	23.225228555585574	24.51580339243314	26.737377902264942	25.52159014971635
45	22.191290006113775	24.496751169436823	26.01381997071076	27.298138853738646
46	22.965322110531332	25.34357982739255	26.296190977208422	25.3949070848677
47	19.932890677207002	25.644718695349265	29.34739027199181	25.07500035545192
48	21.17483969118337	25.387798046436238	28.087953023474043	25.349409238906347
49	23.185275759600756	23.839165114526608	28.476533064137744	24.49902606173489
50	21.532566505054525	24.38968905065901	28.096910411897685	25.980834032388778
51	20.679908435585006	24.474428788762033	25.959080374788506	28.88658240086446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	303.0
1	573.0
2	843.0
3	1652.0
4	2461.0
5	1667.0
6	873.0
7	903.0
8	933.0
9	983.5
10	1034.0
11	1045.0
12	1056.0
13	1094.5
14	1133.0
15	1089.0
16	1045.0
17	1155.0
18	1265.0
19	1161.5
20	1058.0
21	1443.5
22	1829.0
23	1971.0
24	2113.0
25	2363.5
26	3314.5
27	4015.0
28	5237.5
29	6460.0
30	7419.0
31	8378.0
32	9196.0
33	10014.0
34	12819.5
35	15625.0
36	16899.0
37	18173.0
38	20439.0
39	22705.0
40	24450.0
41	26195.0
42	30418.0
43	34641.0
44	38876.0
45	43111.0
46	49582.0
47	56053.0
48	61633.5
49	67214.0
50	69040.5
51	70867.0
52	64837.5
53	58808.0
54	52834.5
55	46861.0
56	43675.5
57	40490.0
58	38902.0
59	37314.0
60	34188.5
61	31063.0
62	27594.5
63	24126.0
64	20818.0
65	17510.0
66	15536.5
67	13563.0
68	12259.5
69	10956.0
70	9625.5
71	8295.0
72	6606.0
73	4917.0
74	4052.0
75	2581.0
76	1975.0
77	1522.0
78	1069.0
79	861.0
80	653.0
81	471.5
82	290.0
83	240.0
84	190.0
85	113.5
86	37.0
87	22.5
88	8.0
89	5.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	703330.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.014834072002593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.85769742413585	21.267820309096034
2	11.057843907589875	6.637987001608299
3	4.077577234928829	3.6716341236649184
4	2.133441919365541	2.5613962084804585
5	1.3145633883601011	1.972820098937897
6	0.9464744906486083	1.7044964874120692
7	0.700062851828098	1.470858923631531
8	0.5716353236172017	1.3726031510452654
9	0.5199918365784675	1.404672182432873
>10	7.6289532365694965	51.306728767085495
>50	0.14489474083075948	2.7388599929299517
>100	0.04255984136423305	2.296210154348086
>500	9.56400929533327E-4	0.28203856440142444
>1k	0.003347403253366644	1.3118740349257096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	1766	0.2510912373992294	Illumina PCR Primer Index 8 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1496	0.21270242986933585	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1374	0.19535637609656917	No Hit
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	1174	0.16692022237072215	RNA PCR Primer, Index 40 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	1169	0.16620931852757598	RNA PCR Primer, Index 40 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	1109	0.15767847240982186	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG	1052	0.14957416859795544	No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	998	0.14189640709197673	RNA PCR Primer, Index 40 (96% over 26bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	967	0.13748880326447047	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.843615372584704E-4	0.0	0.0	0.1470149147626292	0.0
2	2.843615372584704E-4	0.0	0.0	0.7009511893421296	0.0
3	2.843615372584704E-4	0.0	0.0	1.0246967995108982	0.0
4	2.843615372584704E-4	0.0	0.0	1.4826610552656647	0.0
5	2.843615372584704E-4	0.0	0.0	2.8275489457296006	0.0
6	2.843615372584704E-4	0.0	0.0	3.5823866463822105	0.0
7	2.843615372584704E-4	0.0	0.0	4.3268451509248855	0.0
8	2.843615372584704E-4	0.0	0.0	5.474386134531443	0.0
9	4.2654230588770565E-4	0.0	0.0	5.928084967227333	0.0
10	5.687230745169408E-4	0.0	0.0	7.056715908606202	0.0
11	5.687230745169408E-4	0.0	0.0	8.461461902663046	0.0
12	5.687230745169408E-4	0.0	0.0	9.656206901454508	0.0
13	5.687230745169408E-4	0.0	0.0	10.114171157209276	0.0
14	5.687230745169408E-4	0.0	0.0	10.335404433196366	0.0
15	5.687230745169408E-4	0.0	0.0	10.705500973938266	0.0
16	5.687230745169408E-4	0.0	0.0	11.40574125943725	0.0
17	5.687230745169408E-4	0.0	0.0	12.195413248404021	0.0
18	5.687230745169408E-4	0.0	0.0	12.999872037308233	0.0
19	5.687230745169408E-4	0.0	0.0	13.581107019464547	0.0
20	7.109038431461761E-4	0.0	0.0	14.131773136365576	0.0
21	8.530846117754113E-4	0.0	0.0	14.797036952781767	0.0
22	9.952653804046465E-4	0.0	0.0	15.500831757496481	0.0
23	9.952653804046465E-4	0.0	0.0	16.197944066085622	0.0
24	9.952653804046465E-4	0.0	0.0	16.749605448367053	0.0
25	9.952653804046465E-4	0.0	0.0	17.29543741913469	0.0
26	9.952653804046465E-4	0.0	0.0	17.76904155943867	0.0
27	9.952653804046465E-4	0.0	0.0	18.299518007194347	0.0
28	9.952653804046465E-4	0.0	0.0	18.799709951231996	0.0
29	0.0011374461490338817	0.0	0.0	19.31980720287774	0.0
30	0.0011374461490338817	0.0	0.0	20.01478679993744	0.0
31	0.0011374461490338817	0.0	0.0	20.560618770705073	0.0
32	0.0011374461490338817	0.0	0.0	21.095929364594145	0.0
33	0.0011374461490338817	0.0	0.0	21.615742254702628	0.0
34	0.0011374461490338817	0.0	0.0	22.148351413987744	0.0
35	0.0012796269176631168	0.0	0.0	22.733709638434306	0.0
36	0.0012796269176631168	0.0	0.0	23.23646083630728	0.0
37	0.0012796269176631168	0.0	0.0	23.750870857207854	0.0
38	0.0012796269176631168	0.0	0.0	24.330826212446503	0.0
39	0.0014218076862923521	0.0	0.0	25.147086005146942	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	55	1.8189894E-12	45.000004	1
TTCACGG	40	6.8084773E-9	45.0	2
CGCTAAT	20	7.031417E-4	45.0	15
GCGATAT	20	7.031417E-4	45.0	9
GTTCACG	35	1.2110286E-7	45.0	1
TATAGCG	40	6.8084773E-9	45.0	1
TTACGCG	35	1.2110286E-7	45.0	1
TACGTAG	40	6.8084773E-9	45.0	1
GCTATCG	25	3.8892573E-5	44.999996	1
GTCCGAC	25	3.8892573E-5	44.999996	26
ACATACG	105	0.0	40.714287	17
GCGTAAG	45	1.9264917E-8	40.0	1
ACGGGTA	85	0.0	39.705883	5
TAGTAGG	120	0.0	39.375004	2
GTTTACG	40	3.4556615E-7	39.375	1
GTTACGG	40	3.4556615E-7	39.375	2
CGTAAGG	80	0.0	39.375	2
GCTACGA	40	3.4556615E-7	39.375	10
TACGGTT	75	0.0	39.000004	33
ACGGGTG	75	0.0	39.000004	5
>>END_MODULE