SRR2934522.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934522.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819415
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2618	0.31949622596608557	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2289	0.27934563072435825	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1665	0.20319374187682676	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	1369	0.1670704099876131	TruSeq Adapter, Index 23 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	1366	0.16670429513738458	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT	1013	0.12362478109382913	TruSeq Adapter, Index 20 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	853	0.10409865574830825	TruSeq Adapter, Index 20 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	20	7.0321007E-4	45.0	1
TATAGCG	25	3.8898223E-5	45.0	1
CACACGA	25	3.8898223E-5	45.0	34
CGACTCA	20	7.0321007E-4	45.0	34
AATACGA	20	7.0321007E-4	45.0	15
TATACGG	30	2.1647375E-6	44.999996	2
GCGATAT	55	6.002665E-11	40.909092	9
GGCTACG	45	1.9272193E-8	40.0	1
ACGGGTA	85	0.0	39.705883	5
ATTAGTC	75	0.0	39.0	31
CGGTAGT	205	0.0	38.414635	12
CGTAAGG	165	0.0	38.181816	2
CGTTGAT	65	9.094947E-12	38.07692	25
CGTTAGG	90	0.0	37.5	2
ACACGAA	30	1.1396362E-4	37.499996	35
AATGCCG	30	1.1396362E-4	37.499996	1
CGTACTG	30	1.1396362E-4	37.499996	38
CGATACG	30	1.1396362E-4	37.499996	10
CGTTTTT	1090	0.0	37.362385	1
TACGGGA	205	0.0	37.317074	4