##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934522.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819415
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21460188060995	33.0	31.0	34.0	30.0	34.0
2	32.33623133577003	34.0	31.0	34.0	30.0	34.0
3	32.37904358597292	34.0	31.0	34.0	30.0	34.0
4	35.91969514836804	37.0	35.0	37.0	35.0	37.0
5	35.93737971601691	37.0	35.0	37.0	35.0	37.0
6	35.94969826034427	37.0	35.0	37.0	35.0	37.0
7	36.13988760274098	37.0	35.0	37.0	35.0	37.0
8	35.93901991054594	37.0	36.0	37.0	35.0	37.0
9	37.67670594265421	39.0	38.0	39.0	35.0	39.0
10	37.28611997583642	39.0	37.0	39.0	34.0	39.0
11	37.18197372515758	39.0	37.0	39.0	34.0	39.0
12	36.998465978777546	39.0	37.0	39.0	34.0	39.0
13	36.94052342219754	39.0	37.0	39.0	33.0	39.0
14	37.93997180915653	40.0	37.0	41.0	33.0	41.0
15	38.15390614035623	40.0	37.0	41.0	33.0	41.0
16	38.31849917319063	40.0	37.0	41.0	34.0	41.0
17	38.32327453122044	40.0	37.0	41.0	34.0	41.0
18	38.31650750840539	40.0	37.0	41.0	34.0	41.0
19	38.282183020813626	40.0	37.0	41.0	34.0	41.0
20	38.147787140826075	40.0	36.0	41.0	34.0	41.0
21	38.10352141466778	40.0	36.0	41.0	34.0	41.0
22	38.121779562248676	40.0	36.0	41.0	34.0	41.0
23	38.130931213121556	40.0	36.0	41.0	34.0	41.0
24	38.03769396459669	40.0	36.0	41.0	34.0	41.0
25	37.95314950299909	40.0	36.0	41.0	34.0	41.0
26	37.798863823581456	40.0	35.0	41.0	34.0	41.0
27	37.75875716212176	40.0	35.0	41.0	33.0	41.0
28	37.75600763959654	40.0	35.0	41.0	33.0	41.0
29	37.73844633061391	40.0	35.0	41.0	33.0	41.0
30	37.60707577967208	40.0	35.0	41.0	33.0	41.0
31	37.61018409475052	40.0	35.0	41.0	33.0	41.0
32	37.55130062300544	40.0	35.0	41.0	33.0	41.0
33	37.51734347064674	40.0	35.0	41.0	33.0	41.0
34	37.438352971327106	40.0	35.0	41.0	33.0	41.0
35	37.38444378001379	40.0	35.0	41.0	33.0	41.0
36	37.2732730057419	40.0	35.0	41.0	33.0	41.0
37	37.2165618154415	39.0	35.0	41.0	33.0	41.0
38	37.12499283025085	39.0	35.0	41.0	32.0	41.0
39	37.05941921980926	39.0	35.0	41.0	32.0	41.0
40	36.97760719537719	39.0	35.0	41.0	32.0	41.0
41	36.843422441619936	39.0	35.0	41.0	32.0	41.0
42	36.82539372601185	39.0	35.0	41.0	32.0	41.0
43	36.75939664272682	39.0	35.0	41.0	32.0	41.0
44	36.66387971906787	39.0	35.0	41.0	32.0	41.0
45	36.53771776206196	39.0	35.0	41.0	31.0	41.0
46	36.49171665151358	38.0	35.0	41.0	31.0	41.0
47	36.44379953991567	38.0	35.0	41.0	31.0	41.0
48	36.364195188030486	38.0	35.0	41.0	31.0	41.0
49	36.315431130745715	38.0	35.0	41.0	31.0	41.0
50	36.190283311874936	38.0	35.0	40.0	31.0	41.0
51	35.21551716773552	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	12.0
10	19.0
11	15.0
12	15.0
13	15.0
14	21.0
15	29.0
16	61.0
17	128.0
18	207.0
19	331.0
20	540.0
21	906.0
22	1243.0
23	1871.0
24	2746.0
25	3920.0
26	5207.0
27	6480.0
28	7677.0
29	9054.0
30	11083.0
31	14272.0
32	19250.0
33	29327.0
34	58582.0
35	65094.0
36	60721.0
37	90746.0
38	156187.0
39	273385.0
40	258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.378367493882834	20.537456600135464	23.777573024657837	11.30660288132387
2	34.677422307377824	22.77417425846488	24.254254559655365	18.294148874501932
3	33.54832410927308	23.829073180256646	25.45364680900398	17.16895590146629
4	29.678978295491294	25.946559435694976	24.697863719848918	19.67659854896481
5	25.916904132826467	29.25526137549349	24.77133076646144	20.0565037252186
6	27.51633787519145	33.9414094201351	24.117083529103077	14.425169175570376
7	83.75231109999207	4.937424870181776	7.545749101493138	3.764514928333018
8	84.2984324182496	4.00102512158064	7.418219095330206	4.28232336483955
9	78.78791576917679	6.087025499899319	9.406954961771508	5.718103769152383
10	44.513829988467386	24.396429159827438	16.71948890366908	14.3702519480361
11	33.54185608025237	25.65037252186011	22.392560546243356	18.41521085164416
12	28.951752164654053	22.651525783638327	28.251740571017127	20.144981480690493
13	26.07689632237633	23.779037484058748	30.27354881226241	19.870517381302513
14	21.98214579913719	24.352129262949788	32.09106496708017	21.574659970832847
15	21.56990047777988	24.184204584978307	33.03625147208679	21.209643465155022
16	26.303399376384373	24.3189348498624	28.76478951447069	20.612876259282537
17	26.126565903723996	23.641256262089417	26.70087806544913	23.531299768737455
18	27.153640096898396	22.758187243338234	28.953216624054967	21.134956035708402
19	25.783516289059875	25.337100248347905	26.564683341164123	22.314700121428093
20	26.588358768145564	27.880133998035184	26.14511572280224	19.386391511017006
21	25.40263480653881	26.228589908654342	28.829713881244544	19.539061403562297
22	25.191996729374004	22.210235350829556	28.794078702488974	23.80368921730747
23	23.86275574647767	25.206153170249507	28.56196188744409	22.369129195828734
24	23.54643251588023	24.83784163091962	29.341176327013784	22.274549526186366
25	24.49247328887072	25.50368250520188	27.309605023095745	22.694239182831655
26	23.179585435951257	26.12046398955352	26.716621004008957	23.983329570486262
27	23.6333237736678	24.673211986600197	27.985941189751223	23.70752304998078
28	20.87123130526046	26.265323431960606	27.97093048089185	24.89251478188708
29	23.487976178127077	25.005766308891097	27.42883642598683	24.077421086994992
30	25.383840910893746	22.872293038326124	28.400505238493317	23.343360812286814
31	24.362380478756187	24.628545974872317	27.082613815954065	23.92645973041743
32	25.553108009982733	23.488708407827534	27.159253857935234	23.798929724254496
33	24.697985758132326	23.456856415857654	26.94593093853542	24.8992268874746
34	22.224879944838698	23.723265988540604	30.025567020374293	24.02628704624641
35	23.2982066474253	24.747167186346356	29.604656980894905	22.34996918533344
36	25.285966207599326	25.119750065595575	26.983518729825544	22.61076499697955
37	23.128329356919267	25.0494560143517	28.358157954150215	23.464056674578813
38	23.24267922847397	24.31673816076103	28.68753928107247	23.753043329692524
39	23.57913877583398	23.174825942898288	27.359030527876595	25.88700475339114
40	25.471342360098365	22.02229639437892	29.747685849050846	22.758675396471872
41	22.68264554590775	23.098185900917116	29.017774875978596	25.20139367719654
42	23.563639913840973	23.300159259959848	29.073668409780147	24.06253241641903
43	23.389247206848786	22.56487860241758	29.441369757692986	24.604504433040645
44	23.725340639358567	22.947834735756604	27.920894784693957	25.405929840190865
45	22.986642909880832	21.98666121562334	27.815697784394967	27.210998090100862
46	23.371063502620775	22.281871823190937	28.651782064033487	25.695282610154806
47	21.306785938748984	23.85970478939243	31.14587846207355	23.68763080978503
48	22.28809577564482	24.16187157911437	29.6289425992934	23.921090045947413
49	24.068878407156326	22.64078641469829	29.38425584105734	23.906079337088045
50	22.043897170542397	22.98383602936241	29.557672241782246	25.414594558312942
51	21.71939737495652	22.899141460676216	27.44933885759963	27.93212230676763
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	655.0
2	1052.0
3	2479.0
4	3906.0
5	2641.5
6	1377.0
7	1352.5
8	1328.0
9	1333.5
10	1339.0
11	1360.5
12	1382.0
13	1506.0
14	1630.0
15	1532.0
16	1434.0
17	1394.0
18	1354.0
19	1548.0
20	1742.0
21	1855.5
22	1969.0
23	2168.5
24	2368.0
25	2806.0
26	4018.0
27	4792.0
28	5660.0
29	6528.0
30	7972.0
31	9416.0
32	11208.5
33	13001.0
34	15551.0
35	18101.0
36	19899.5
37	21698.0
38	24433.5
39	27169.0
40	29259.0
41	31349.0
42	34626.5
43	37904.0
44	42513.0
45	47122.0
46	54504.5
47	61887.0
48	68541.5
49	75196.0
50	77767.0
51	80338.0
52	73386.5
53	66435.0
54	59978.0
55	53521.0
56	50313.5
57	47106.0
58	45911.5
59	44717.0
60	39902.5
61	35088.0
62	31435.5
63	27783.0
64	25701.0
65	23619.0
66	21281.5
67	18944.0
68	16649.5
69	14355.0
70	12052.0
71	9749.0
72	8649.0
73	7549.0
74	6206.0
75	4259.5
76	3656.0
77	2666.5
78	1677.0
79	1257.0
80	837.0
81	563.0
82	289.0
83	204.0
84	119.0
85	123.0
86	127.0
87	97.0
88	67.0
89	46.0
90	25.0
91	14.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	819415.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.895577372095044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.72810890551126	21.14457652166677
2	10.583384907715034	6.327928047345156
3	4.014176813543596	3.6001840054368754
4	2.1271314294932204	2.543672889241188
5	1.2998102762723507	1.9429289341672147
6	1.0308052528255476	1.8489910914844891
7	0.7448212869691574	1.5586803689078899
8	0.6327928479078347	1.5134166036109638
9	0.5510602878664763	1.4826838925341093
>10	8.12368668053811	51.98198110702674
>50	0.11470734851249575	2.169797623271038
>100	0.04621303198858771	2.4258805952641334
>500	8.252327140819233E-4	0.18626460382634197
>1k	0.00247569814224577	1.2730137162171187
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2618	0.31949622596608557	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2289	0.27934563072435825	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1665	0.20319374187682676	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	1369	0.1670704099876131	TruSeq Adapter, Index 23 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	1366	0.16670429513738458	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT	1013	0.12362478109382913	TruSeq Adapter, Index 20 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	853	0.10409865574830825	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6611485022851667E-4	0.0	0.0	0.1291165038472569	0.0
2	3.6611485022851667E-4	0.0	0.0	0.5684543241214769	0.0
3	3.6611485022851667E-4	0.0	0.0	0.8816045593502682	0.0
4	4.8815313363802227E-4	0.0	0.0	1.2565061659842693	0.0
5	4.8815313363802227E-4	0.0	0.0	2.388045129757205	0.0
6	4.8815313363802227E-4	0.0	0.0	3.2323059743841642	0.0
7	6.101914170475279E-4	0.0	0.0	3.947694391730686	0.0
8	6.101914170475279E-4	0.0	0.0	5.02285166856843	0.0
9	6.101914170475279E-4	0.0	0.0	5.513445567874642	0.0
10	6.101914170475279E-4	0.0	0.0	6.474497049724499	0.0
11	6.101914170475279E-4	0.0	0.0	7.790435859729197	0.0
12	6.101914170475279E-4	0.0	0.0	8.746361733675855	0.0
13	6.101914170475279E-4	0.0	0.0	9.190092932152817	0.0
14	6.101914170475279E-4	0.0	0.0	9.438928992024799	0.0
15	6.101914170475279E-4	0.0	0.0	9.783809180940061	0.0
16	6.101914170475279E-4	0.0	0.0	10.392414100303265	0.0
17	6.101914170475279E-4	0.0	0.0	11.087544162603809	0.0
18	6.101914170475279E-4	0.0	0.0	11.808668379270577	0.0
19	7.322297004570333E-4	0.0	0.0	12.287546603369478	0.0
20	8.542679838665389E-4	0.0	0.0	12.772038588505215	0.0
21	8.542679838665389E-4	0.0	0.0	13.358310502004478	0.0
22	8.542679838665389E-4	0.0	0.0	13.988272120964346	0.0
23	8.542679838665389E-4	0.0	0.0	14.61200978747033	0.0
24	8.542679838665389E-4	0.0	0.0	15.130672491960729	0.0
25	8.542679838665389E-4	0.0	0.0	15.617117089631018	0.0
26	8.542679838665389E-4	0.0	0.0	16.06804854682914	0.0
27	8.542679838665389E-4	0.0	0.0	16.565232513439465	0.0
28	8.542679838665389E-4	0.0	0.0	17.020313272273512	0.0
29	8.542679838665389E-4	0.0	0.0	17.52189061708658	0.0
30	8.542679838665389E-4	0.0	0.0	18.270961600654125	0.0
31	9.763062672760445E-4	0.0	0.0	18.799021252967055	0.0
32	9.763062672760445E-4	0.0	0.0	19.306944588517418	0.0
33	9.763062672760445E-4	0.0	0.0	19.80534893796184	0.0
34	0.0010983445506855501	0.0	0.0	20.31058743127719	0.0
35	0.0010983445506855501	0.0	0.0	20.862810663705204	0.0
36	0.0010983445506855501	0.0	0.0	21.334732705649763	0.0
37	0.0010983445506855501	0.0	0.0	21.819712843919138	0.0
38	0.0012203828340950557	0.0	0.0	22.38487213438856	0.0
39	0.001342421117504561	0.0	0.0	23.30296614047827	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	20	7.0321007E-4	45.0	1
TATAGCG	25	3.8898223E-5	45.0	1
CACACGA	25	3.8898223E-5	45.0	34
CGACTCA	20	7.0321007E-4	45.0	34
AATACGA	20	7.0321007E-4	45.0	15
TATACGG	30	2.1647375E-6	44.999996	2
GCGATAT	55	6.002665E-11	40.909092	9
GGCTACG	45	1.9272193E-8	40.0	1
ACGGGTA	85	0.0	39.705883	5
ATTAGTC	75	0.0	39.0	31
CGGTAGT	205	0.0	38.414635	12
CGTAAGG	165	0.0	38.181816	2
CGTTGAT	65	9.094947E-12	38.07692	25
CGTTAGG	90	0.0	37.5	2
ACACGAA	30	1.1396362E-4	37.499996	35
AATGCCG	30	1.1396362E-4	37.499996	1
CGTACTG	30	1.1396362E-4	37.499996	38
CGATACG	30	1.1396362E-4	37.499996	10
CGTTTTT	1090	0.0	37.362385	1
TACGGGA	205	0.0	37.317074	4
>>END_MODULE