##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934521.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 746432 Sequences flagged as poor quality 0 Sequence length 51 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19725440495584 33.0 31.0 34.0 30.0 34.0 2 32.31781997556375 34.0 31.0 34.0 30.0 34.0 3 32.37507234416531 34.0 31.0 34.0 30.0 34.0 4 35.908657453056676 37.0 35.0 37.0 35.0 37.0 5 35.936823448083686 37.0 35.0 37.0 35.0 37.0 6 35.95217380819686 37.0 35.0 37.0 35.0 37.0 7 36.121447097659264 37.0 35.0 37.0 35.0 37.0 8 35.90409039269485 37.0 35.0 37.0 35.0 37.0 9 37.65443469733345 39.0 38.0 39.0 35.0 39.0 10 37.27229004115579 39.0 37.0 39.0 34.0 39.0 11 37.20085419703335 39.0 37.0 39.0 34.0 39.0 12 37.016655234502274 39.0 37.0 39.0 34.0 39.0 13 36.97929081282689 39.0 37.0 39.0 33.0 39.0 14 38.03236597573523 40.0 37.0 41.0 33.0 41.0 15 38.20724057918203 40.0 37.0 41.0 33.0 41.0 16 38.353137325302235 40.0 37.0 41.0 34.0 41.0 17 38.35169981994341 40.0 37.0 41.0 34.0 41.0 18 38.33367004630026 40.0 37.0 41.0 34.0 41.0 19 38.31794188887936 40.0 37.0 41.0 34.0 41.0 20 38.19531049043985 40.0 37.0 41.0 34.0 41.0 21 38.15533096115922 40.0 36.0 41.0 34.0 41.0 22 38.162016097916485 40.0 36.0 41.0 34.0 41.0 23 38.1827520792249 40.0 36.0 41.0 34.0 41.0 24 38.097158214010115 40.0 36.0 41.0 34.0 41.0 25 38.02052430763954 40.0 36.0 41.0 34.0 41.0 26 37.85973806053331 40.0 36.0 41.0 34.0 41.0 27 37.81902035282518 40.0 35.0 41.0 33.0 41.0 28 37.823452102803735 40.0 36.0 41.0 34.0 41.0 29 37.8122628719026 40.0 36.0 41.0 34.0 41.0 30 37.69226533696305 40.0 36.0 41.0 33.0 41.0 31 37.69509479765069 40.0 35.0 41.0 33.0 41.0 32 37.6259257373746 40.0 35.0 41.0 33.0 41.0 33 37.57915389479551 40.0 35.0 41.0 33.0 41.0 34 37.49994239260911 40.0 35.0 41.0 33.0 41.0 35 37.45356844293921 40.0 35.0 41.0 33.0 41.0 36 37.36844347509217 40.0 35.0 41.0 33.0 41.0 37 37.293476163937235 39.0 35.0 41.0 33.0 41.0 38 37.21620723656006 39.0 35.0 41.0 33.0 41.0 39 37.16617722712853 39.0 35.0 41.0 33.0 41.0 40 37.0617578024522 39.0 35.0 41.0 32.0 41.0 41 36.96020802966647 39.0 35.0 41.0 32.0 41.0 42 36.91947156606362 39.0 35.0 41.0 32.0 41.0 43 36.83616056117637 39.0 35.0 41.0 32.0 41.0 44 36.73977133884935 39.0 35.0 41.0 32.0 41.0 45 36.627241329417814 39.0 35.0 41.0 32.0 41.0 46 36.56279607519506 38.0 35.0 41.0 32.0 41.0 47 36.528802355740375 38.0 35.0 41.0 32.0 41.0 48 36.458682103661154 38.0 35.0 41.0 31.0 41.0 49 36.4007826566921 38.0 35.0 40.0 31.0 41.0 50 36.26574691331562 38.0 35.0 40.0 31.0 41.0 51 35.302314209465834 37.0 34.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 13.0 10 19.0 11 14.0 12 15.0 13 19.0 14 30.0 15 28.0 16 51.0 17 104.0 18 164.0 19 295.0 20 478.0 21 810.0 22 1171.0 23 1675.0 24 2349.0 25 3277.0 26 4378.0 27 5271.0 28 6455.0 29 7820.0 30 9516.0 31 12465.0 32 16935.0 33 25184.0 34 51867.0 35 64024.0 36 55021.0 37 83261.0 38 143632.0 39 249849.0 40 235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24711695104176 21.31245712938352 25.363864357369458 11.076561562205264 2 32.43403283889222 24.227123167281146 24.617781659950268 18.72106233387636 3 32.7883852782303 24.29893144988425 25.39092643402212 17.52175683786333 4 28.727868044242477 25.43192146103061 25.68994898396639 20.150261510760526 5 25.83678084540856 29.960130326674093 24.370739732487355 19.832349095429993 6 27.28286032753151 33.594084926691245 23.732503429649316 15.390551316127926 7 82.51843436508616 5.377047071936895 7.521247749292635 4.583270813684301 8 83.75525165051873 4.411788347766441 7.31814820372117 4.514811797993655 9 78.39441503043814 6.293674440538455 9.200570179199177 6.11134034982423 10 45.31370573608848 22.601389007973935 16.564670324959273 15.520234930978308 11 32.81785882705993 23.81087627540084 22.668508316899597 20.70275658063963 12 29.18497599245477 21.019731201234674 28.690490225499442 21.104802580811114 13 25.988301680528163 23.33420860841979 30.219497556374858 20.457992154677186 14 21.712493569407528 25.345778316042182 31.205923647432048 21.735804467118236 15 20.756076909885966 23.262266355140188 34.19306782131527 21.78858891365858 16 24.794355011575067 22.612374603446796 30.485831261253537 22.1074391237246 17 25.14964524564863 22.79377089942553 27.125980665351968 24.930603189573866 18 25.59469583297608 22.318041027179973 29.168363628568976 22.918899511274972 19 25.08882255851839 25.135176412586812 26.378290319814802 23.39771070908 20 27.00045014147304 25.88527823030095 26.54588763611421 20.56838399211181 21 25.397624967847037 25.791364786075622 28.14991854582869 20.661091700248647 22 24.470681857155103 22.71379040555603 28.087890122609966 24.727637614678898 23 23.781804638600704 24.64082461630798 28.107851753408212 23.4695189916831 24 23.989861099202606 24.150491940324102 28.1568850210066 23.70276193946669 25 23.636714395953014 25.181530266655233 26.35323780330961 24.82851753408214 26 22.823914301637657 25.292457986795853 26.658021092343308 25.225606619223186 27 22.987358526965618 24.39994533996399 28.318721598216584 24.29397453485381 28 21.11793170710795 24.98486131355569 28.348998971105203 25.54820800823116 29 23.801096416016463 24.35734266483752 27.39485981308411 24.446701106061905 30 24.839235188202004 23.30795035582612 28.003756537769014 23.849057918202867 31 25.080650347251993 24.711963045528595 26.974057918202867 23.233328689016545 32 27.289424890679925 24.593265026151077 25.322601389007975 22.794708694161024 33 25.896263825773815 23.264677827317158 26.24592729143445 24.593131055474576 34 23.395165266226527 23.287452842321873 29.476764125868133 23.84061776558347 35 24.38453871216668 25.35877347166252 27.149961416445166 23.106726399725627 36 25.898139415244792 25.0558657720998 26.630959015690646 22.415035796964762 37 23.851469390379833 25.21971190945726 28.429649318357196 22.49916938180571 38 24.33724706336277 25.55838977964503 26.077392180399556 24.026970976592644 39 24.602910914858956 23.73585269656178 26.362749721340993 25.298486667238272 40 25.02866972477064 22.253735102460773 28.656461673668865 24.061133499099718 41 22.673197290577036 24.05765026151076 27.57545228500386 25.693700162908346 42 24.31246248821058 24.129458544113863 28.22681771413873 23.331261253536827 43 24.36631870016291 22.74513954385664 28.31255894709766 24.57598280888279 44 24.05135363971534 22.41570565034725 27.874742776301126 25.658197933636284 45 23.76050330103747 22.24596480322387 27.099052559375803 26.89447933636286 46 24.43986860156049 22.78198147989368 27.82999657035068 24.94815334819515 47 21.58240804252765 23.82132598816771 30.27147817885621 24.324787790448426 48 23.345060233216152 23.728082397324872 28.967541584497987 23.95931578496099 49 24.88398139415245 21.960875203635428 29.46135749807082 23.6937859041413 50 22.86356962188116 22.466078624710622 28.944364657463773 25.72598709594444 51 21.786713324187602 23.40199777072794 27.002191760267515 27.809097144816942 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 335.0 1 646.5 2 958.0 3 1659.5 4 2361.0 5 1709.0 6 1057.0 7 1046.0 8 1035.0 9 1083.5 10 1132.0 11 1136.0 12 1140.0 13 1144.0 14 1148.0 15 1152.0 16 1156.0 17 1202.5 18 1249.0 19 1294.5 20 1340.0 21 1526.5 22 1713.0 23 1888.5 24 2064.0 25 2232.5 26 3612.0 27 4823.0 28 5554.0 29 6285.0 30 7529.5 31 8774.0 32 9875.0 33 10976.0 34 12849.0 35 14722.0 36 16927.5 37 19133.0 38 20357.5 39 21582.0 40 23930.5 41 26279.0 42 30542.0 43 34805.0 44 38021.0 45 41237.0 46 49259.0 47 57281.0 48 63955.5 49 70630.0 50 71392.0 51 72154.0 52 65463.0 53 58772.0 54 53374.5 55 47977.0 56 45594.0 57 43211.0 58 41824.5 59 40438.0 60 38140.0 61 35842.0 62 32811.0 63 29780.0 64 27702.5 65 25625.0 66 21963.0 67 18301.0 68 15460.5 69 12620.0 70 10949.5 71 9279.0 72 7781.5 73 6284.0 74 5625.0 75 3884.0 76 2802.0 77 2206.0 78 1610.0 79 1124.0 80 638.0 81 477.5 82 317.0 83 222.5 84 128.0 85 87.5 86 47.0 87 30.5 88 14.0 89 9.5 90 5.0 91 3.5 92 2.0 93 1.0 94 0.0 95 1.5 96 3.0 97 2.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 746432.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.56839348827908 #Duplication Level Percentage of deduplicated Percentage of total 1 70.19690560607926 20.75609726620135 2 11.422352181552922 6.754812077317196 3 4.205958777452515 3.730903323815916 4 2.1087253168843305 2.4940647971332752 5 1.2906796758753014 1.908166226180271 6 0.9223755646983232 1.6363898184584182 7 0.7180914309595294 1.486296699382093 8 0.5695178314225382 1.3471781870474402 9 0.4802659346574577 1.2780622921471056 >10 7.895256813473001 52.50521652925728 >50 0.1432038105023247 2.6615255078967115 >100 0.04117681450809238 2.0320613318745187 >500 0.003202641128407185 0.5457250125012854 >1k 0.0022876008060051323 0.8635009307872347 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC 1590 0.2130133756323416 No Hit GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC 1493 0.20001822001200378 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1163 0.15580789676755552 No Hit CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT 1078 0.14442038926519762 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 1059 0.1418749464117294 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.679413529966561E-4 0.0 0.0 0.14910936294263913 0.0 2 2.679413529966561E-4 0.0 0.0 0.7096426734116437 0.0 3 2.679413529966561E-4 0.0 0.0 1.029564648889651 0.0 4 2.679413529966561E-4 0.0 0.0 1.4770267083940667 0.0 5 2.679413529966561E-4 0.0 0.0 2.802264640315528 0.0 6 4.0191202949498413E-4 0.0 0.0 3.6055528165995026 0.0 7 4.0191202949498413E-4 0.0 0.0 4.371731115493441 0.0 8 4.0191202949498413E-4 0.0 0.0 5.531649232615965 0.0 9 4.0191202949498413E-4 0.0 0.0 5.938652147817885 0.0 10 4.0191202949498413E-4 0.0 0.0 6.932178684729487 0.0 11 4.0191202949498413E-4 0.0 0.0 8.439616736688674 0.0 12 4.0191202949498413E-4 0.0 0.0 9.678845494298209 0.0 13 4.0191202949498413E-4 0.0 0.0 10.15712080939724 0.0 14 4.0191202949498413E-4 0.0 0.0 10.349502700848838 0.0 15 4.0191202949498413E-4 0.0 0.0 10.698228371773986 0.0 16 4.0191202949498413E-4 0.0 0.0 11.383488382062934 0.0 17 4.0191202949498413E-4 0.0 0.0 12.20271906885021 0.0 18 4.0191202949498413E-4 0.0 0.0 13.045528594701192 0.0 19 4.0191202949498413E-4 0.0 0.0 13.618387207408043 0.0 20 4.0191202949498413E-4 0.0 0.0 14.158021092343308 0.0 21 4.0191202949498413E-4 0.0 0.0 14.865252293577981 0.0 22 4.0191202949498413E-4 0.0 0.0 15.587086298550973 0.0 23 4.0191202949498413E-4 0.0 0.0 16.27569557575238 0.0 24 4.0191202949498413E-4 0.0 0.0 16.882314798936807 0.0 25 4.0191202949498413E-4 0.0 0.0 17.434943839492412 0.0 26 4.0191202949498413E-4 0.0 0.0 17.937869759067134 0.0 27 4.0191202949498413E-4 0.0 0.0 18.451379362085227 0.0 28 4.0191202949498413E-4 0.0 0.0 18.93514747492069 0.0 29 4.0191202949498413E-4 0.0 0.0 19.472503858355484 0.0 30 4.0191202949498413E-4 0.0 0.0 20.120520020577896 0.0 31 5.358827059933122E-4 0.0 0.0 20.667656263397067 0.0 32 5.358827059933122E-4 0.0 0.0 21.17218983108977 0.0 33 6.698533824916402E-4 0.0 0.0 21.667077510074595 0.0 34 6.698533824916402E-4 0.0 0.0 22.186481822858614 0.0 35 6.698533824916402E-4 0.0 0.0 22.764967203978394 0.0 36 6.698533824916402E-4 0.0 0.0 23.27968254308497 0.0 37 6.698533824916402E-4 0.0 0.0 23.81248392351882 0.0 38 6.698533824916402E-4 0.0 0.0 24.381591357283718 0.0 39 6.698533824916402E-4 0.0 0.0 25.192917774157593 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCC 30 2.1644737E-6 45.000004 12 ATAACGC 30 2.1644737E-6 45.000004 11 ACGATTC 20 7.031696E-4 45.0 30 GTATACG 25 3.889487E-5 45.0 1 CCAACGG 40 6.8102963E-9 45.0 2 CGGTATG 20 7.031696E-4 45.0 37 CCGTAGG 55 6.002665E-11 40.909092 2 TAGACGG 95 0.0 40.26316 2 CGTTAGG 95 0.0 40.26316 2 CGTAAGG 95 0.0 40.26316 2 TGTTACG 40 3.4560435E-7 39.375 1 CCGGTAT 40 3.4560435E-7 39.375 42 TCTTGCG 35 6.2453455E-6 38.571426 1 ACCGGTA 35 6.2453455E-6 38.571426 41 TCGTAAG 35 6.2453455E-6 38.571426 1 GTTTGCG 60 1.546141E-10 37.500004 1 CGTATGT 30 1.13953814E-4 37.500004 36 CGGCCTA 30 1.13953814E-4 37.500004 44 TGCGCAC 30 1.13953814E-4 37.500004 13 CGAATAT 30 1.13953814E-4 37.500004 14 >>END_MODULE