SRR2934520.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934520.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	810162
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2773	0.3422772235676321	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1818	0.22439956453153814	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1250	0.1542901296283953	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	1227	0.15145119124323284	Illumina PCR Primer Index 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	1102	0.1360221782803933	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	25	3.8897822E-5	45.000004	1
CACGAAC	25	3.8897822E-5	45.000004	25
AATACCG	20	7.032052E-4	45.0	1
ACCGGTA	30	2.1647065E-6	44.999996	41
GCGTTAG	60	0.0	44.999996	1
TATCGCG	30	2.1647065E-6	44.999996	1
TACCGGT	30	2.1647065E-6	44.999996	40
CCATACG	60	3.6379788E-12	41.249996	2
CGTTTTT	1825	0.0	41.178085	1
GTGATAG	110	0.0	40.909092	1
CGAACCC	50	1.0804797E-9	40.500004	19
TTGTACG	40	3.4565346E-7	39.375	1
CACACGG	40	3.4565346E-7	39.375	2
CGGTATT	40	3.4565346E-7	39.375	43
CGTTAGG	120	0.0	39.374996	2
TGTACGG	105	0.0	38.57143	2
AATAGGG	395	0.0	38.164555	3
TCGTTTG	30	1.1396246E-4	37.499996	1
CGACATT	30	1.1396246E-4	37.499996	20
GTAATCG	30	1.1396246E-4	37.499996	42