##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934520.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	810162
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.119724203307484	33.0	31.0	34.0	30.0	34.0
2	32.23934225500579	33.0	31.0	34.0	30.0	34.0
3	32.29280810504566	34.0	31.0	34.0	30.0	34.0
4	35.85599546757315	37.0	35.0	37.0	35.0	37.0
5	35.889112300996594	37.0	35.0	37.0	35.0	37.0
6	35.908823173636875	37.0	35.0	37.0	35.0	37.0
7	36.10618493585233	37.0	35.0	37.0	35.0	37.0
8	35.89968179203665	37.0	35.0	37.0	35.0	37.0
9	37.63199705737865	39.0	38.0	39.0	35.0	39.0
10	37.18192410900536	39.0	37.0	39.0	34.0	39.0
11	37.11104569209615	39.0	37.0	39.0	34.0	39.0
12	36.9891626612949	39.0	37.0	39.0	33.0	39.0
13	36.9519034464712	39.0	37.0	39.0	33.0	39.0
14	38.04932346863961	40.0	37.0	41.0	33.0	41.0
15	38.23276826116258	40.0	37.0	41.0	33.0	41.0
16	38.35400944502457	40.0	37.0	41.0	34.0	41.0
17	38.35981939414586	40.0	37.0	41.0	34.0	41.0
18	38.315491222743105	40.0	37.0	41.0	34.0	41.0
19	38.25603274406847	40.0	37.0	41.0	34.0	41.0
20	38.1275683134978	40.0	36.0	41.0	34.0	41.0
21	38.097291652780555	40.0	36.0	41.0	34.0	41.0
22	38.108405479397945	40.0	36.0	41.0	34.0	41.0
23	38.13442866982159	40.0	36.0	41.0	34.0	41.0
24	38.02576645164794	40.0	36.0	41.0	34.0	41.0
25	37.9291684872902	40.0	36.0	41.0	34.0	41.0
26	37.78309029552114	40.0	36.0	41.0	33.0	41.0
27	37.719821467805204	40.0	35.0	41.0	33.0	41.0
28	37.71850691590077	40.0	35.0	41.0	33.0	41.0
29	37.700236495910694	40.0	35.0	41.0	33.0	41.0
30	37.56784569012124	40.0	35.0	41.0	33.0	41.0
31	37.55611840594844	40.0	35.0	41.0	33.0	41.0
32	37.46849272120885	40.0	35.0	41.0	33.0	41.0
33	37.389256716557924	40.0	35.0	41.0	33.0	41.0
34	37.29133679436952	40.0	35.0	41.0	33.0	41.0
35	37.25361841212005	40.0	35.0	41.0	33.0	41.0
36	37.163142680105956	40.0	35.0	41.0	32.0	41.0
37	37.122120020440356	40.0	35.0	41.0	32.0	41.0
38	37.0228448137533	39.0	35.0	41.0	32.0	41.0
39	36.95233175587105	39.0	35.0	41.0	32.0	41.0
40	36.8496399485535	39.0	35.0	41.0	32.0	41.0
41	36.758653207630076	39.0	35.0	41.0	31.0	41.0
42	36.706417975664124	39.0	35.0	41.0	31.0	41.0
43	36.624766404743745	39.0	35.0	41.0	31.0	41.0
44	36.51364295042226	39.0	35.0	41.0	31.0	41.0
45	36.3801486616257	39.0	35.0	41.0	31.0	41.0
46	36.29212058822803	38.0	35.0	41.0	31.0	41.0
47	36.25135960462229	38.0	35.0	41.0	31.0	41.0
48	36.174140480545866	38.0	35.0	40.0	31.0	41.0
49	36.10626886968285	38.0	35.0	40.0	31.0	41.0
50	35.96295185407363	38.0	35.0	40.0	30.0	41.0
51	34.91103631125627	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	11.0
10	15.0
11	18.0
12	19.0
13	17.0
14	31.0
15	33.0
16	63.0
17	105.0
18	163.0
19	297.0
20	553.0
21	841.0
22	1372.0
23	2023.0
24	3066.0
25	4500.0
26	6178.0
27	7369.0
28	8176.0
29	9440.0
30	11273.0
31	14644.0
32	19160.0
33	27955.0
34	54855.0
35	67819.0
36	60084.0
37	90594.0
38	156884.0
39	262343.0
40	255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15692417072141	20.84015295706291	24.818369659401455	11.184553212814228
2	33.30815318417798	22.988982450423496	25.551679787499292	18.151184577899233
3	31.69724079875383	23.520851385278498	27.46253218492104	17.31937563104663
4	27.550539274861073	26.186614528946063	26.60727113836492	19.65557505782794
5	24.891194600586054	29.50200083440102	26.058615437406345	19.548189127606577
6	26.39385703106292	34.06084215255714	24.769984274749987	14.775316541629946
7	81.90596448611512	5.176618997188217	8.382397594555163	4.535018922141497
8	83.11658655923137	3.892307958161454	8.160343239993976	4.830762242613205
9	77.67804463798599	5.8998076927824314	10.560233632285888	5.861914036945697
10	42.77576583448742	23.421118245486706	18.19142344370632	15.61169247631955
11	31.32694448764568	24.22169887010252	24.24120114248755	20.210155499764245
12	27.468827222209878	21.17885064962316	29.94055509885677	21.411767029310187
13	24.60927567572905	23.332493995028155	31.549986299036487	20.508244030206306
14	21.41818549870273	25.476139340033228	31.749822874931187	21.35585228633286
15	19.904784475203723	24.33229403502016	34.55099103636063	21.21193045341549
16	23.607130425766698	23.300278215961747	31.163767246550687	21.928824111720864
17	23.59034365966313	23.608364746803726	28.183499102648607	24.61779249088454
18	24.389072802723405	22.930969361683218	31.166976481246962	21.512981354346415
19	24.112091162014508	25.87704681285965	27.735317134104044	22.275544891021795
20	24.60507898420316	26.176246232235034	27.830483286058836	21.388191497502966
21	23.010706500675173	26.00887229961415	30.21988194953602	20.760539250174656
22	22.613872287270944	23.624534352388782	28.62217679920806	25.139416561132215
23	21.36547999042167	25.10806480679173	29.242299688210505	24.284155514576096
24	21.805885736432963	24.229598524739497	30.74706046445032	23.217455274377226
25	22.62436401608567	25.507367662270013	28.024641985183212	23.843626336461103
26	21.812797934240315	25.895931924726167	27.919971561243308	24.371298579790217
27	21.977826656890844	25.34394355696762	28.434930297891036	24.243299488250496
28	19.89367558587048	25.367025360360028	28.984079727264422	25.75521932650507
29	21.849827565351127	23.391741404805458	28.72684722314796	26.03158380669545
30	23.171661963903514	25.136083894332245	27.337742328077596	24.354511813686646
31	23.00613951283817	26.633562176453594	25.439726869440925	24.920571441267303
32	23.908181326697623	25.52119205788472	24.865891019326998	25.70473559609066
33	22.525371468916095	26.182170973212767	25.45577304292228	25.83668451494886
34	21.39053670747332	25.71744910277204	26.689476919430927	26.20253727032371
35	21.47879066162076	24.840217141756835	27.610650709364297	26.0703414872581
36	23.04600808233415	27.93108045057655	24.91649818184511	24.106413285244184
37	20.766587423256087	27.758769233807563	26.496552541343583	24.978090801592767
38	20.100671223779937	27.903431659347145	26.820685245666915	25.175211871206006
39	21.17564141492689	26.446192243032872	26.09663252534678	26.281533816693454
40	22.71607406913679	23.99705244136358	28.491091905075777	24.795781584423857
41	21.25834092440771	24.693456370454303	27.427724331676874	26.62047837346111
42	21.48459197049479	25.524648156788395	28.08833295069381	24.902426922023004
43	21.468792661220842	25.063258953147642	28.226330042633446	25.241618342998066
44	21.233160775252358	24.634826121195513	26.986948289354473	27.145064814197656
45	21.631723038108426	23.960146242356466	26.357074264159515	28.05105645537559
46	22.785689775625123	24.735299853609526	26.941772139399276	25.53723823136607
47	20.177569424386725	25.553284404847425	28.99938530812356	25.269760862642286
48	20.401351828399754	25.170768315472706	28.214727424885393	26.213152431242147
49	21.484715402598493	24.140233681658728	29.34314371693563	25.03190719880715
50	20.716720853360194	23.64156798269976	28.834109721265623	26.80760144267443
51	19.817147681574795	24.000385108163552	26.731567266793554	29.450899943468094
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	379.0
1	666.5
2	954.0
3	2836.0
4	4718.0
5	3237.5
6	1757.0
7	1774.5
8	1792.0
9	1858.0
10	1924.0
11	1976.5
12	2029.0
13	2041.0
14	2053.0
15	2046.0
16	2039.0
17	1921.5
18	1804.0
19	1989.0
20	2174.0
21	2308.0
22	2442.0
23	2588.0
24	2734.0
25	3498.0
26	4577.0
27	4892.0
28	5692.5
29	6493.0
30	7761.0
31	9029.0
32	10779.5
33	12530.0
34	15133.0
35	17736.0
36	19463.0
37	21190.0
38	23319.5
39	25449.0
40	28868.0
41	32287.0
42	36459.0
43	40631.0
44	44738.5
45	48846.0
46	57076.0
47	65306.0
48	76314.0
49	87322.0
50	85497.5
51	83673.0
52	73701.5
53	63730.0
54	56587.5
55	49445.0
56	45219.0
57	40993.0
58	38340.0
59	35687.0
60	33725.5
61	31764.0
62	28706.5
63	25649.0
64	23542.5
65	21436.0
66	19209.0
67	16982.0
68	15099.5
69	13217.0
70	11057.0
71	8897.0
72	7532.0
73	6167.0
74	5068.0
75	3288.5
76	2608.0
77	2071.0
78	1534.0
79	1234.5
80	935.0
81	614.0
82	293.0
83	291.0
84	289.0
85	176.0
86	63.0
87	53.0
88	43.0
89	28.0
90	13.0
91	7.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	810162.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.49803798211645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.42528737585637	23.536797400062795
2	11.447226088126413	7.44024776403613
3	4.15441287789459	4.050308024976363
4	2.025885762224062	2.63349249792746
5	1.2198315098908286	1.9821065370107305
6	0.8575414045666231	1.6721047882106166
7	0.6373629251611691	1.4499131185196363
8	0.5121289444311005	1.3314548710290486
9	0.4119184451852296	1.204788714944755
>10	6.075541226168442	46.993815672789516
>50	0.18406202757081203	3.6873966817654313
>100	0.044958781985402735	2.5844743885659627
>500	0.0019213154694616532	0.4128465562716982
>1k	0.0019213154694616532	1.0202529838898289
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2773	0.3422772235676321	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1818	0.22439956453153814	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1250	0.1542901296283953	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	1227	0.15145119124323284	Illumina PCR Primer Index 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	1102	0.1360221782803933	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2343210370271623E-4	0.0	0.0	0.16058516691723385	0.0
2	1.2343210370271623E-4	0.0	0.0	0.7065253615943478	0.0
3	1.2343210370271623E-4	0.0	0.0	1.1074328344207702	0.0
4	1.2343210370271623E-4	0.0	0.0	1.665963103675561	0.0
5	1.2343210370271623E-4	0.0	0.0	3.128880396760154	0.0
6	1.2343210370271623E-4	0.0	0.0	4.174844043536972	0.0
7	1.2343210370271623E-4	0.0	0.0	5.055285239248446	0.0
8	1.2343210370271623E-4	0.0	0.0	6.522892952273743	0.0
9	1.2343210370271623E-4	0.0	0.0	7.0457513435584485	0.0
10	1.2343210370271623E-4	0.0	0.0	8.307227443400208	0.0
11	1.2343210370271623E-4	0.0	0.0	10.12772754091157	0.0
12	1.2343210370271623E-4	0.0	0.0	11.464250359804582	0.0
13	1.2343210370271623E-4	0.0	0.0	12.040061123577754	0.0
14	1.2343210370271623E-4	0.0	0.0	12.27890224424251	0.0
15	1.2343210370271623E-4	0.0	0.0	12.68203149493558	0.0
16	1.2343210370271623E-4	0.0	0.0	13.523221281669592	0.0
17	1.2343210370271623E-4	0.0	0.0	14.546349989261406	0.0
18	1.2343210370271623E-4	0.0	0.0	15.596386895460414	0.0
19	1.2343210370271623E-4	0.0	0.0	16.269832453262435	0.0
20	1.2343210370271623E-4	0.0	0.0	16.93031764017567	0.0
21	1.2343210370271623E-4	0.0	0.0	17.80285918125017	0.0
22	1.2343210370271623E-4	0.0	0.0	18.70440726669481	0.0
23	1.2343210370271623E-4	0.0	0.0	19.638911723828073	0.0
24	1.2343210370271623E-4	0.0	0.0	20.355064789511232	0.0
25	1.2343210370271623E-4	0.0	0.0	20.99024639516541	0.0
26	1.2343210370271623E-4	0.0	0.0	21.591977900716152	0.0
27	1.2343210370271623E-4	0.0	0.0	22.16976357814857	0.0
28	1.2343210370271623E-4	0.0	0.0	22.76236110802531	0.0
29	2.4686420740543247E-4	0.0	0.0	23.397789577886893	0.0
30	2.4686420740543247E-4	0.0	0.0	24.16874649761406	0.0
31	3.7029631110814873E-4	0.0	0.0	24.830836301875426	0.0
32	3.7029631110814873E-4	0.0	0.0	25.440467462063143	0.0
33	3.7029631110814873E-4	0.0	0.0	26.03466960928802	0.0
34	3.7029631110814873E-4	0.0	0.0	26.591101532779863	0.0
35	3.7029631110814873E-4	0.0	0.0	27.24887121341164	0.0
36	3.7029631110814873E-4	0.0	0.0	27.84912153371795	0.0
37	3.7029631110814873E-4	0.0	0.0	28.4513467676835	0.0
38	3.7029631110814873E-4	0.0	0.0	29.074555459278514	0.0
39	3.7029631110814873E-4	0.0	0.0	29.84415462586495	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	25	3.8897822E-5	45.000004	1
CACGAAC	25	3.8897822E-5	45.000004	25
AATACCG	20	7.032052E-4	45.0	1
ACCGGTA	30	2.1647065E-6	44.999996	41
GCGTTAG	60	0.0	44.999996	1
TATCGCG	30	2.1647065E-6	44.999996	1
TACCGGT	30	2.1647065E-6	44.999996	40
CCATACG	60	3.6379788E-12	41.249996	2
CGTTTTT	1825	0.0	41.178085	1
GTGATAG	110	0.0	40.909092	1
CGAACCC	50	1.0804797E-9	40.500004	19
TTGTACG	40	3.4565346E-7	39.375	1
CACACGG	40	3.4565346E-7	39.375	2
CGGTATT	40	3.4565346E-7	39.375	43
CGTTAGG	120	0.0	39.374996	2
TGTACGG	105	0.0	38.57143	2
AATAGGG	395	0.0	38.164555	3
TCGTTTG	30	1.1396246E-4	37.499996	1
CGACATT	30	1.1396246E-4	37.499996	20
GTAATCG	30	1.1396246E-4	37.499996	42
>>END_MODULE