##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934433.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2075658
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.023105925928064	33.0	31.0	34.0	30.0	34.0
2	32.219468717871635	34.0	31.0	34.0	30.0	34.0
3	32.2753358212191	34.0	31.0	34.0	30.0	34.0
4	35.834707837225594	37.0	35.0	37.0	35.0	37.0
5	35.80098021928468	37.0	35.0	37.0	35.0	37.0
6	35.46708224572642	37.0	35.0	37.0	33.0	37.0
7	35.845063589473796	37.0	35.0	37.0	35.0	37.0
8	35.93631320766716	37.0	35.0	37.0	35.0	37.0
9	37.726615367271485	39.0	38.0	39.0	35.0	39.0
10	37.13110300444485	39.0	37.0	39.0	33.0	39.0
11	37.020693678823775	39.0	37.0	39.0	33.0	39.0
12	36.91163910432258	39.0	37.0	39.0	33.0	39.0
13	36.853254726934786	39.0	37.0	39.0	33.0	39.0
14	37.88740967924388	40.0	37.0	41.0	33.0	41.0
15	37.93668706501745	40.0	37.0	41.0	33.0	41.0
16	37.980365744260375	40.0	37.0	41.0	33.0	41.0
17	37.9390838953238	40.0	37.0	41.0	33.0	41.0
18	37.901508822744404	39.0	37.0	41.0	33.0	41.0
19	37.93253271974478	40.0	37.0	41.0	33.0	41.0
20	37.828611457186106	40.0	36.0	41.0	33.0	41.0
21	37.68988147373026	39.0	36.0	41.0	33.0	41.0
22	37.79381670776207	39.0	36.0	41.0	33.0	41.0
23	37.7328957853365	39.0	36.0	41.0	33.0	41.0
24	37.67988608913414	39.0	36.0	41.0	33.0	41.0
25	37.600167272257764	39.0	36.0	41.0	33.0	41.0
26	37.5321271616037	39.0	36.0	41.0	33.0	41.0
27	37.46429951369638	39.0	36.0	41.0	33.0	41.0
28	37.37801554976783	39.0	36.0	41.0	32.0	41.0
29	37.32843079158513	39.0	36.0	41.0	32.0	41.0
30	37.29581992794574	39.0	36.0	41.0	32.0	41.0
31	37.203050309829464	39.0	35.0	41.0	32.0	41.0
32	37.0699450487508	39.0	35.0	41.0	31.0	41.0
33	36.96349687665309	39.0	35.0	41.0	31.0	41.0
34	36.78192168459351	39.0	35.0	41.0	31.0	41.0
35	36.75333364166929	39.0	35.0	41.0	31.0	41.0
36	36.57268538458648	39.0	35.0	41.0	30.0	41.0
37	36.50907519446845	39.0	35.0	41.0	30.0	41.0
38	36.44224385712868	39.0	35.0	40.0	30.0	41.0
39	36.35389356049985	39.0	35.0	40.0	30.0	41.0
40	36.20081921010109	39.0	35.0	40.0	30.0	41.0
41	36.12833713453758	39.0	35.0	40.0	30.0	41.0
42	36.07966823050811	39.0	35.0	40.0	29.0	41.0
43	35.979030745912866	38.0	35.0	40.0	29.0	41.0
44	35.85058232136508	38.0	35.0	40.0	29.0	41.0
45	35.938352560971026	38.0	35.0	40.0	29.0	41.0
46	35.846220331095004	38.0	35.0	40.0	29.0	41.0
47	35.62161059288187	38.0	35.0	40.0	28.0	41.0
48	35.5428943496472	38.0	34.0	40.0	27.0	41.0
49	35.55125748076032	38.0	34.0	40.0	27.0	41.0
50	35.44318428180365	38.0	34.0	40.0	27.0	41.0
51	34.52804556434634	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	25.0
10	29.0
11	16.0
12	15.0
13	17.0
14	32.0
15	34.0
16	93.0
17	179.0
18	396.0
19	785.0
20	1396.0
21	2415.0
22	3818.0
23	6068.0
24	9234.0
25	14149.0
26	19333.0
27	22998.0
28	26165.0
29	30861.0
30	38427.0
31	48136.0
32	61784.0
33	83928.0
34	128750.0
35	156151.0
36	186851.0
37	271522.0
38	451182.0
39	510699.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.810886957292578	4.541692321182006	57.637048107154456	15.010372614370961
2	21.003845527538736	4.712818778430743	53.544418203769595	20.73891749026092
3	19.21101645839536	4.623594060293169	54.09739947525074	22.06799000606073
4	18.396238686720068	4.587942715033016	53.30748129027037	23.708337307976553
5	17.472435247039733	5.490066282595688	53.073820446335574	23.963678024029004
6	20.381199600319512	6.169706184737563	55.22080227089434	18.228291944048586
7	81.53115783043256	1.7851206701682067	11.759740766542464	4.923980732856761
8	83.3716344407412	2.370477217345054	9.268386217768052	4.989502124145693
9	78.47704197897727	4.67716743317059	11.422739198846823	5.423051389005318
10	35.68111895119523	34.38707147323885	17.037440657372265	12.894368918193653
11	23.69903905171276	25.293906799675092	30.845013966655394	20.16204018195676
12	23.343874568931877	22.15066258506941	33.80576183552396	20.69970101047475
13	23.259563955140973	23.20329264262224	33.862129503029884	19.675013899206903
14	19.169631991397427	25.976726416394225	33.130409730312024	21.723231861896323
15	16.94397631979835	26.769487073496695	35.11411802907801	21.17241857762695
16	19.88135810427344	27.0762331752148	31.934788871769822	21.10761984874194
17	20.15987219474499	26.92172795325627	30.13690116579899	22.78149868619975
18	19.735813896123542	26.103385047054957	32.71984112989712	21.44095992692438
19	20.593710524566188	27.564174830342957	31.271432962462985	20.57068168262787
20	21.64272727009941	29.45051641455384	30.644402883326638	18.262353432020113
21	22.240465433130122	27.828717447672012	31.52537653120119	18.405440587996676
22	20.140553019813474	25.5885603505009	31.118710307767465	23.15217632191816
23	19.45898601792781	26.960366303119297	30.382654560626076	23.197993118326814
24	20.110104843861563	26.77184777068284	30.42153379795708	22.69651358749852
25	19.58752357083874	28.263615682352295	27.962024572448836	24.18683617436013
26	18.42986657724924	28.612035316029903	28.83904766584861	24.119050440872243
27	18.301521734312686	27.481984026270222	31.959889345932712	22.25660489348438
28	15.968912026933147	26.266995815302906	32.617945730944115	25.146146426819833
29	17.09848154175688	24.315710969726226	32.45939359952362	26.126413888993273
30	19.939122919093606	25.72586620724609	32.477122917166504	21.857887956493798
31	18.261004462199455	29.040092346619723	29.681575673834516	23.017327517346306
32	18.695661809411764	28.022631859391094	27.889902864537415	25.39180346665973
33	20.104323544630184	26.176807547293436	29.79782796587877	23.921040942197607
34	17.578714797909868	25.48218444464358	30.61202760763093	26.32707314981563
35	17.562141740113255	24.797148663219083	28.797133246421136	28.843576350246526
36	18.850311563851076	26.049811674177537	30.74123964545219	24.358637116519198
37	17.642694509403764	27.602138695295658	32.5615780634382	22.19358873186238
38	17.820373105781396	26.19058631046155	30.78879083163026	25.200249752126798
39	18.981980653845675	25.728226904432233	30.94079082392186	24.349001617800234
40	19.76101072527362	26.1410116695525	30.699855178454253	23.398122426719624
41	18.34459241358644	26.439230354904325	27.768447403184922	27.44772982832432
42	20.55353049490812	24.33700542189513	28.922635617235592	26.186828465961153
43	20.377827175767877	23.968640305869272	30.653604784603246	24.99992773375961
44	19.30289093868065	24.00120829153936	29.26657474400889	27.429326025771104
45	19.534191085429295	23.655149355047893	28.495349426543292	28.315310132979516
46	19.38344370797116	24.87341363557966	30.83060889607055	24.912533760378636
47	16.393885697932898	25.46055275001951	32.764116246510746	25.381445305536847
48	17.1158736169446	24.304774678680207	32.90522812524992	25.67412357912527
49	19.946686785587993	22.897317380801656	32.48752925578299	24.66846657782737
50	18.7861391423828	22.537335148661292	30.8606716520737	27.815854056882205
51	17.38812463324883	22.47118744995563	28.94147301723116	31.19921489956438
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	468.0
1	1175.0
2	1882.0
3	11445.0
4	21008.0
5	13221.5
6	5435.0
7	5671.0
8	5907.0
9	6755.5
10	7604.0
11	8372.5
12	9141.0
13	9386.5
14	9632.0
15	9592.5
16	9553.0
17	9471.5
18	9390.0
19	9134.0
20	8878.0
21	8760.0
22	8642.0
23	9961.5
24	11281.0
25	13112.0
26	18246.5
27	21550.0
28	24670.0
29	27790.0
30	31156.5
31	34523.0
32	40606.0
33	46689.0
34	53986.5
35	61284.0
36	68347.0
37	75410.0
38	81934.5
39	88459.0
40	98648.5
41	108838.0
42	123855.5
43	138873.0
44	149188.5
45	159504.0
46	187197.0
47	214890.0
48	238441.5
49	261993.0
50	238623.0
51	215253.0
52	177427.5
53	139602.0
54	117225.5
55	94849.0
56	82772.5
57	70696.0
58	63832.0
59	56968.0
60	51736.5
61	46505.0
62	39315.0
63	32125.0
64	25931.0
65	19737.0
66	16283.0
67	12829.0
68	10477.5
69	8126.0
70	7308.5
71	6491.0
72	5375.0
73	4259.0
74	3358.5
75	1676.5
76	895.0
77	805.0
78	715.0
79	506.5
80	298.0
81	225.0
82	152.0
83	106.0
84	60.0
85	53.5
86	47.0
87	30.0
88	13.0
89	8.5
90	4.0
91	4.0
92	4.0
93	4.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2075658.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.09506728456578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.99932355698971	21.06634352321695
2	12.61967907453708	7.595801817156405
3	5.4034903401148355	4.878552160717716
4	2.7863610750719836	3.3542289613354557
5	1.6343841046274965	2.459344979879465
6	1.0568961677914093	1.9084416769489312
7	0.6935294489075067	1.4610270800089467
8	0.5291343555974073	1.2739467227423462
9	0.41583063443814217	1.1263005830159605
>10	3.941214093298461	27.706015339835645
>50	0.805886499783371	15.853750657292531
>100	0.10311856699200014	5.66236673063965
>500	0.006303350611312824	1.274295982702302
>1k	0.00404060936622617	2.055080741628073
>5k	6.464974985961871E-4	1.5526195804205465
>10k+	1.6162437464904678E-4	0.7718834624590742
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15869	0.7645286458559166	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	9898	0.4768608316013524	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT	9689	0.4667917354400388	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	7160	0.34495085413878396	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	5173	0.24922217436591193	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCC	4397	0.21183643933634538	No Hit
TCCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTG	3266	0.15734769408062407	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT	2734	0.1317172674881893	No Hit
TGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTG	2284	0.11003739537052828	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8906496156881335E-4	0.0	0.0	0.5095733497522231	0.0
2	2.8906496156881335E-4	0.0	0.0	1.716853161744372	0.0
3	3.372424551636156E-4	0.0	0.0	2.5060486843208274	0.0
4	3.372424551636156E-4	0.0	0.0	3.526833418607497	0.0
5	3.372424551636156E-4	0.0	0.0	5.126229850967741	0.0
6	3.372424551636156E-4	0.0	0.0	6.179341683456523	0.0
7	3.372424551636156E-4	0.0	0.0	7.176760333349714	0.0
8	3.372424551636156E-4	0.0	0.0	8.672093379545185	0.0
9	3.854199487584178E-4	0.0	0.0	9.39104611646042	0.0
10	3.854199487584178E-4	0.0	0.0	10.777160784676473	0.0
11	3.854199487584178E-4	0.0	0.0	13.61857300191072	0.0
12	3.854199487584178E-4	0.0	0.0	15.947521219777054	0.0
13	3.854199487584178E-4	0.0	0.0	16.653273323447312	0.0
14	4.3359744235322006E-4	0.0	0.0	16.877443201143926	0.0
15	4.3359744235322006E-4	0.0	0.0	17.202063152985705	0.0
16	4.817749359480223E-4	0.0	0.0	17.9528612131671	0.0
17	4.817749359480223E-4	0.0	0.0	19.113216146397914	0.0
18	4.817749359480223E-4	0.0	0.0	20.394159346096515	0.0
19	4.817749359480223E-4	0.0	0.0	21.328369124393326	0.0
20	4.817749359480223E-4	0.0	0.0	22.263879695017195	0.0
21	4.817749359480223E-4	0.0	0.0	23.58047423997595	0.0
22	5.299524295428245E-4	0.0	0.0	25.026473532730343	0.0
23	5.781299231376267E-4	0.0	0.0	26.368843036762318	0.0
24	5.781299231376267E-4	0.0	0.0	27.371079436014988	0.0
25	6.263074167324289E-4	0.0	0.0	28.242369407676986	0.0
26	6.263074167324289E-4	0.0	0.0	28.9772688949721	0.0
27	6.263074167324289E-4	0.0	0.0	29.739244133667494	0.0
28	6.263074167324289E-4	0.0	0.0	30.503724602029813	0.0
29	6.744849103272312E-4	0.0	0.0	31.322163863218314	0.0
30	6.744849103272312E-4	0.0	0.0	32.272320391895	0.0
31	6.744849103272312E-4	0.0	0.0	33.08232859170441	0.0
32	6.744849103272312E-4	0.0	0.0	33.84757989996425	0.0
33	6.744849103272312E-4	0.0	0.0	34.58252756475296	0.0
34	6.744849103272312E-4	0.0	0.0	35.34320201112129	0.0
35	6.744849103272312E-4	0.0	0.0	36.19223398074249	0.0
36	6.744849103272312E-4	0.0	0.0	36.96808433759319	0.0
37	6.744849103272312E-4	0.0	0.0	37.717196185498764	0.0
38	7.226624039220334E-4	0.0	0.0	38.487795195547626	0.0
39	7.226624039220334E-4	0.0	0.0	39.449177080231905	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCGT	25	3.8918955E-5	45.000004	23
ACGGGTA	25	3.8918955E-5	45.000004	5
CGTAGCA	35	1.2125565E-7	45.000004	17
TCGCTAT	25	3.8918955E-5	45.000004	25
ATAGGCG	20	7.0346025E-4	45.0	13
CGTTTTT	6790	0.0	43.608246	1
TCGTTCA	200	0.0	41.625004	16
CGGGTAT	425	0.0	40.764706	6
TTCCGCG	230	0.0	40.1087	1
GCGATTC	450	0.0	39.5	9
TACGGGC	80	0.0	39.375	4
TCGAACG	40	3.460027E-7	39.375	22
TGGGCGA	2505	0.0	39.251495	6
TAGTGCG	75	0.0	39.0	1
TTTGTCG	595	0.0	38.94958	1
TTTTGCG	1930	0.0	38.704662	1
TTTCGCG	665	0.0	38.571426	1
TTCTTCG	735	0.0	38.265305	1
TTTGGGA	14945	0.0	38.179993	4
TTGGGAC	6590	0.0	38.069042	5
>>END_MODULE